Browse > Article
http://dx.doi.org/10.5352/JLS.2011.21.10.1452

Phylogenic Study of Genus Citrus and Two Relative Genera in Korea by trnL-trnF Sequence  

Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
Yoon, Hye-Jeong (Department of Science Education, Busan National University)
Choi, Joo-Soo (Department of Molecular Biology, Dongeui University)
Publication Information
Journal of Life Science / v.21, no.10, 2011 , pp. 1452-1459 More about this Journal
Abstract
Citrus is a common term and genus (Citrus) of flowering plants in the rue family, Rutaceae. Citrus is believed to have originated in the part of Southeast Asia bordered by Northeastern India, Myanmar (Burma) and the Yunnan province of China. The taxonomy and systematics of the genus are complex and the precise number of natural species is unclear, as many of the named species are clonally propagated hybrids, and there is genetic evidence that even some wild, true-breeding species are of a hybrid origin. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the chloroplast trnL-trnF region. We evaluated the seven taxa with the trnL-trnF region to estimate phenotypic relationships within the genera Citrus, Poncirus, and Fortunella in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within Citrus was mostly due to insertion/deletion. Within the genus Citrus, C. lomonia and C. sinensis were relatives and sistered with C. aurantium in the four phylogenetic analyses (MP, ML, ME, and NJ). However, some external nodes were poorly supported.
Keywords
Citrus; trnL-trnF; phylogenetic analyses;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Woo, H. S. 1997. Studies on RAPD analysis hybrid plants of interspecific and intergeneric among Citrus and Poncirus triforiata (L.) Raf. MS. Thesis, Chonnam National University, Gwangju, Korea.
2 Yun, S. H. 2001. Classification of genus Citrus and its related genera using RAPD. MS. Thesis, Jeju Natl. Univ., Jeju, Korea.
3 Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70I, 3321-3323.
4 Nei, M. and S. Kumar. 2000. Molecular Evolution and Phylogenetics. Oxford University Press, New York.
5 Nicolosi, E., Z. N. Deng, A. Gentile, S. La Malfa, G. Continella, and E. Tribulato. 2000. Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor. Appl. Genet. 100, 1155-1166.   DOI
6 Oh, J. B. and D. K. Moon. 2002. RAPD and phylogenic relationship of Citrus growing in Jeju Island. J. Subtropical Agri. Biotech. 18, 1-10.
7 Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
8 Scora, R. W. 1975. On the history and origin of citrus. Bulletin of the Torrey Botanical Club 102, 369-375.   DOI
9 Swofford, D. L. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Version 4. Sunderland, Sinauer Associates, Inc. MA.
10 Taberlet, P., L. Gielly, G. Pautou, and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol. Biol. 17, 1105-1109.   DOI
11 Tajima, F. 1989. Statistical methods to test for nucleotide mutation hypothesis by DNA polymorphism. Genetics 123, 585-595.
12 Tamura, K., M. Nei, and S. Kumar. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. (USA) 101, 11030-11035.   DOI
13 Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei, and S. Kumar. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol. Biol. Evol. (In Press).
14 Bonnard, G., F. Michel, J. H. Well, and A. Steinmetz. 1984. Nucleotide sequence of the $tRNA^{Leu}$ gene from Vicia faba chloroplasts: evidence for structural homologies of the $tRNA^{Leu}$ intron with intron from the autosplicable Tetrahymena ribosomal RNA precursor. Mol. Gen. Genet. 194, 330-336.   DOI
15 Cech, T. R. 1988. Conserved sequences and structures of group I introns: building an active site for RNA catalysis - a review. Gene 73, 259-271.   DOI
16 Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) version 3.5s, Distributed by the author. Department of Genetics, Univ. Washington, seattle.
17 Freitas, de A. E., de Q. L. Paganucci, and M. A. Machado. 2003. What is Citrus? Taxonomic implications from a study of cp-DNA evolution in the tribe Citreae (Rutaceae subfamily Aurantioideae). Organisms Diversity & Evolution 3, 55-62.   DOI
18 Kumar, S. and S. R. Gadagkar. 2001. Disparity Index: A simple statistic to measure and test the homogeneity of substitution patterns between molecular sequences. Genetics 158, 1321-1327.
19 Gmitter, F. and X. Hu. 1990. The possible role of Yunnan, China, in the origin of contemporary Citrus species (Rutaceae). Economic Botany 44, 267-277.   DOI
20 Hirai, M. and I. Kajiura. 1987. Genetic analysis of leaf isozyme in Citrus. Japan J. Breed. 37, 377-388.   DOI
21 Lee, T. B. 2003. Coloured Flora of Korea. Hyangmoon-sa, Seoul. Korea.
22 Lee, Y. N. 2007. New Flora of Korea. Kyo-Hak Publishing Co., Seoul. Korea.
23 Michel, F. and B. Dujon. 1983. Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members. EMBO J. 2, 33-38.
24 Andrews, A. C. 1961. Acclimatization of citrus fruits in the Mediterranean region. Agricultural History 35, 35-46.