• 농업과학연구
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• 제45권4호
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• pp.583-590
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• 2018

Genus Typha L. (Typhaceae; Cattail in common) is one of the hydrophytic plants found in semi-aquatic regions. About nine to 18 species of the genus exist all over the world. In Korea, the most commonly found cattail species are T. laxmanni and T. angustifolia. The aim of this study was to prepare a cDNA library and sequences and analyze expressed sequence tags (ESTs) from these species, T. laxmanni and T. angustifolia. In the case of T. laxmanni, we observed that 715 out of 742 ESTs had high quality sequences, whereas the remaining 27 ESTs were low quality sequences. In this study, we identified 77 contigs, 393 unassembled clones and 65.7% singletons. Furthermore, in the case of T. angustifolia, we recorded 992 high quality EST sequences, and by excluding 28 low quality sequences from among them, we retrieved 120 contigs, 348 unassembled clones and 48.9% singletons. The basic local alignment search tool (BLAST) and Kyoto encyclopedia of genes and genomes (KEGG) database results enabled us to identify the functional categories, i.e., molecular function (16.5%), biological process (22.2%) and cellular components (61.3%). In addition, between these two species, the no hits and anonymous genes were 4.2% and 11.7% and 6.2% and 11.2% in T. laxmanni and T. angustifolia, respectively, based on the BLAST results. The study concluded that they have certain species-specific genes. Hence, the results of this study on these two species could be a valuable resource for further studies.

• 한국도서관정보학회지
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• 제52권2호
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• pp.107-126
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• 2021

4차산업혁명에 따라 새로운 교육 환경이 도래하는 상황 속에서 충청남도 교육청 소속 19개 공공도서관의 활성화를 위한 새로운 방향을 모색하기 위해 현황분석과 사서, 사서교사, 전문가 대상 포커스그룹인터뷰를 실시하였다. 연구방법을 통해 충청남도 교육청 소속 공공도서관의 비전으로 도서관 커뮤니티 스쿨을 통한 지식체험 에듀라이브러리를 설정하였다. 에듀라이브러리로서 공공도서관 서비스의 방향을 교육기능 특화 서비스, 생애주기 맞춤 서비스, 지식체험중심서비스, 4차산업혁명 지능정보사회 서비스로 나누어 살펴보았다. 도서관의 전통적 기능을 바탕으로 미래 공공도서관이 갖추어야 할 공간을 체험학습 공간으로 제시하였다. 본 연구에서 충남교육청 소속 도서관의 교육기능을 강화한 특화된 방향을 제시하였는데 이는 학교 및 학교도서관과 협력 방안 모색에 기여할 수 있을 것이며, 타 지역 교육청 소속 도서관의 방향성 마련에 기반을 제공할 수 있을 것이다.

• Genomics & Informatics
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• 제19권4호
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• pp.39.1-39.8
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• 2021

Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)-positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on its mechanism of action. To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. For these 13 TAM-sensitive genes, we conducted a comparative analysis of their Gene Ontology (GO) 'biological process' terms identified from other genome-wide screenings of the budding yeast deletion library and the MCF7 breast cancer cell line. Several TAM-sensitive genes overlapped between the yeast strains and MCF7 in GO terms including 'cell cycle' (cdc2, rik1, pas1, and leo1), 'signaling' (sck2, oga1, and cki3), and 'vesicle-mediated transport' (SPCC126.08c, vps54, sec72, and tvp15), suggesting their roles in the ER-independent cytotoxic effects of TAM. We recently reported that the cki3 gene with the 'signaling' GO term was related to the ER-independent antifungal action mechanisms of TAM in yeast. In this study, we report that haploinsufficiency of the essential vps54 gene, which encodes the GARP complex subunit, significantly aggravated TAM sensitivity and led to an enlarged vesicle structure in comparison with the SP286 control strain. These results strongly suggest that the vesicle-mediated transport process might be another action mechanism of the ER-independent antifungal or cytotoxic effects of TAM.

• 한국문헌정보학회지
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• 제11권
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• pp.43-75
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• 1984

Information science is the study how in formation is transferred and all the intermediate steps of collecting, organizing, interpreting, storing, retrieving, disseminating and trans foming information. Professional education means the transfer of knowledge, the development of cognitive abilities and the infusion of professional attitudes. Training may be defined as practice-based instruction in the development and use of professional skills. Each is affected by the confluence of social, economic and technological realities of the environment where the learning takes place. We have witnessed controversy about methods of curriculum revision and change. Should information science courses be added to the traditional library science curriculum or should the new approaches be integrated within the subject matter of each individual course? The article is based upon the assumption that education for librarianship is at a turning point. To provide this information, 25 curricula of colleges and universities were analysed to assist in the study. Also 32 information professionals were asked to assist in the study. In the experimental part of this study, curricula based on the education and training of library and information profession als were examined. The most frequently offered compulsory course 'Introduction to Information Science' exposes students to a new way of looking at library and information problems. Information retrieval, library automation, computer programming, data processing, indexing and abstraction, communication, system analysis has offered. These indicate a curriculum slowly shift from traditional librarianship to an emphasis on computerization and automation. Also from a questionnaire listing 58 events might influence library and information science education.

• 한국문헌정보학회지
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• 제36권4호
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• pp.311-329
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• 2002

본 연구에서는 최신의 정보기술을 적용한 XML 기반 충남대학교 전자도서관 웹사이트를 대상으로 사용성 평가를 실시하였다. 사용성 평가는 로그파일 분석, 온라인 설문조사 그리고 발견평가의 3가지 방법을 적용하였다. 본 연구에서는 사용자 입장에서의 대학도서관 웹사이트 서비스의 문제점을 분석$\cdot$평가하여 개선 방안을 제시함으로써 이용자 서비스의 품질 향상을 제고할 수 있는 근거를 마련하고자 한다.

• 농업과학연구
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• 제43권1호
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• pp.33-39
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• 2016

Brassicaceae consists of important species that have significant amounts of metabolites, and many studies have been carried out in order to understand the mechanism that improves the content of these metabolites. In Brassicacea, Cardamine manshurica Nakai is one of the important edible plants and is rich in oil, fiber, and various nutrients. In this study, we constructed cDNA library using leaves from 4 week-old plants and analyzed the ESTs of C. manshurica Nakai. One thousand thirty-nine ESTs were discovered which assembled to form 468 unigenes. The latter contained 116 contigs and 352 singletons. Similarity search of these ESTs with BLASTX revealed similarities with Arabidopsis thaliana 285 (31.9%), Arabidopsis lyrata 172 (19.3%), Capsella rubella 162 (18.1%), and Eutrema salsugineum 137 (15.3%). ESTs were functionally categorized into molecular function, biological process, and cellular component, and each category took 10.6%, 58.5%, and 30.9%, respectively. The functional analysis also found that 94.9% of ESTs showed at least one GO ID. Microsatellite analysis of 468 unigene sequences revealed 225 structures of which Di-, Tri-, Tetra-, Penta-repeats were 35.6% (80/225), 63.1% (142/225), 0.9% (2/225), and 0.4% (1/225), respectively. The results from our study can be a valuable resource for Cardamine research.

• 농업과학연구
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• 제42권2호
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• pp.81-86
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• 2015

Three genes selected from cDNA library of tobacco whitefly, Bemisia tabaci, were checked whether these genes expressed in plant or not, and confirmed the change of gene expression using qRT-PCR in the tobacco whitefly. First of all, three genes were inserted in Tobacco rattle virus (TRV) RNA2 vector using Sac I and Xho I restriction enzymes, and conducted agro-infiltration in tobacco plants (Nicotiana benthamianana). And then, it was confirmed that TRV RNA2 vector and genes inserted in TRV RNA2 vector were expressed in plant. So, after feeding the tobacco whitefly the plants inoculated the genes and induced RNAi of the genes, we plan to confirm the RNAi in the whitefly and investigate the changes of gene expression through the qRT-PCR.

• 농업과학연구
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• 제44권3호
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• pp.318-324
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• 2017

With the development of next-generation sequencing (NGS), a cutting-edge technology, genotype-by-sequencing (GBS) became available at a low cost per sample. GBS makes it possible to customize the process of library preparation to obtain high-quality single nucleotide polymorphisms (SNPs) in the most efficient way. However, a GBS library is hard to construct due to fine-tuning of concentration of each reagent and set-up. The major reason for this is the presence of undigested genomic DNA (gDNA) owing to the efficiency of different restriction enzymes for different species with unknown reasons. Therefore, this proof-concept study is to demonstrate the unpredictable patterns of enzyme digestion from various plants in order to make the reader aware of the caution needed when choosing restriction enzymes for their GBS library preparations. Indeed, no pattern was found for the digestibility of gDNA samples and restriction enzymes in the current study. We suggest that more data should be accumulated on this matter to help researchers who want to apply GBS technologies in a variety of genetic approaches.

• 한국정보관리학회:학술대회논문집
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• 한국정보관리학회 2004년도 제11회 학술대회 논문집
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• pp.227-235
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• 2004

국내 대학도서관 및 연구기관에서는 컨소시엄에 가입해 전자정보를 구독하고 있다. 저렴한 비용으로 많은 자료를 구독해 예산 절감효과는 있으나 이용 및 활용면에서는 극히 미비한 실정이다. 지역간 전자정보를 공동 활용하는 방안으로는 전자정보를 공동 수서해 근거리에 위치해 있는 대학도서관에서는 상호대차 및 정보공유 협정을 맺어 이용시키는 것이 효율적이며, 이용성활화 방안에 대해서는 천안의 C대학교 홈페이지를 사례로 하여 살펴보았다. 전자정보의 이용활성화는 이용자 편의형 통합 인터페이스를 추축하여 전자정보 원문에 대한 다양한 접근점을 이용할 수 있는 홈페이지를 구현하는 것이 효과적인 방안인 것으로 밝혀졌다.

• 한국정보관리학회:학술대회논문집
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• 한국정보관리학회 2004년도 제11회 학술대회 논문집
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• pp.95-104
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• 2004

품질이 낮은 정보는 이용자의 호응을 얻지 못할 뿐 아니라 여러 측정하기 힘든 직·간접적인 손실들을 초래할 수도 있다. 따라서 질 높은 지식정보자원의 구축이야말로 국가경쟁력을 넘어 국민의 삶의 질을 향상시키는 기본 요소이다. 지식기반시대에 지식정보의 창출-가공-전달-활용보전을 위한 제반요소의 구축뿐 아니라 질적인 향상을 위한 노력은 지식정보시대를 앞서나갈 수 있는 일로 매우 중요한 일이라 할 수 있겠다. 이러한 이유로 인해 근래에 정보 유동·서비스 분야의 품질향상을 위한 품질경영시스템의 도입에 대한 연구가 진행되어지고 있다. 본 연구에서는 지식정보의 유통과 서비스에 있어서 질적인 향상을 위한 노력의 일환으로 지식정보 유통·서비스 야의 품질체계를 확립하는 ISO 9001:2000 인증제도의 도입과 운영에 대한 방향 및 사례를 제시함으로써 지식정보 유통·서비스 품질향상에 도움이 되고자 한다.