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http://dx.doi.org/10.7744/cnujas.2015.42.2.081

Gene expression in plant according to RNAi treatment of the tobacco whitefly  

Kim, Jeong-Hee (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Jung-Kyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Yu, Yong-Man (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Youn, Young-Nam (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.42, no.2, 2015 , pp. 81-86 More about this Journal
Abstract
Three genes selected from cDNA library of tobacco whitefly, Bemisia tabaci, were checked whether these genes expressed in plant or not, and confirmed the change of gene expression using qRT-PCR in the tobacco whitefly. First of all, three genes were inserted in Tobacco rattle virus (TRV) RNA2 vector using Sac I and Xho I restriction enzymes, and conducted agro-infiltration in tobacco plants (Nicotiana benthamianana). And then, it was confirmed that TRV RNA2 vector and genes inserted in TRV RNA2 vector were expressed in plant. So, after feeding the tobacco whitefly the plants inoculated the genes and induced RNAi of the genes, we plan to confirm the RNAi in the whitefly and investigate the changes of gene expression through the qRT-PCR.
Keywords
Bemisia tabaci; cDNA library; RNAi; TRV VIGS vector; qRT-PCR;
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