• Archives of Pharmacal Research
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• 제27권5호
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• pp.507-511
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• 2004

From the flowers of Chromolaena odorata (Eupatorium odoratum) four flavanones, isosakuranetin (5,7-dihydroxy-4'-methoxyflavanone) (1), persicogenin (5,3'-dihydroxy-7,4'-dimethoxyflavanone) (2), 5,6,7,4'-tetramethoxyflavanone (3) and 4'-hydroxy-5,6,7-trimethoxyfla-vanone (4), two chalcones, 2'-hydroxy-4,4',5',6'-tetramethoxychalcone (5) and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone (6), and two flavones, acacetin (5,7-dihydroxy-4'-methoxyflavone) (7) and luteolin (5,7,3',4'-tetrahydroxyflavone) (8) were isolated and identified. Compound 1 exhibited moderate antimycobacterial activity against Mycobacterium tuberculosis with the MIC value of 174.8 $\mu$M, whereas compounds 4,7, and 8 exhibited weak activity with the MIC values of 606.0, 704.2 and 699.3 $\mu$M respectively. Compound 7 showed moderate cytotoxicity against human small cell lung cancer (NCI-H187) cells with the MIC value of 24.6 $\mu$M, whereas compound 8 exhibited moderate toxicity against NCI-H187 cells and week toxicity against human breast cancer (BC) cells with the MIC values of 19.2 and 38.4 $\mu$M respectively.

• Fisheries and Aquatic Sciences
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• 제27권7호
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• pp.434-446
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• 2024

The efficacy of Chromolaena odorata leaf flour components (CO) to increase the growth performance, non-specific immune activity, and resistance against Vibrio parahaemolyticus of whiteleg shrimp (Litopenaeus vannamei) culture was evaluated. To this purpose, whiteleg shrimp post larvae were fed on diets supplemented with 0 and a 1.5 g CO/kg diet for 45 days in a pond. After feeding trials, the shrimps were identified for their growth parameters, collected, and injected with V. parahaemolyticus, then their non-specific immune activity and resistance to V. parahaemolyticus was observed for 14 days. Findings showed that CO increased the average body weight (7.71 g, 37%), weight gain (7.69 g, 40%), and specific growth rate (13.23%/day, 5.7%) as compared to the control. In addition, CO supplementation also increases shrimp's hematologic and immune activity (total hemocyte counts [6.8 × 10⁷ CFU/mL, 242.9%], differential hemocyte counts [27%, 142.1%], and prophenoloxidase activity [0.085%, 566.7%]). Finally, shrimp resistance to V. parahaemolyticus infection also increased after CO supplementation, with survival rates of 73.33% as compared to 23.33% for the control. It suggests that C. odorata leaf flour component supplementation at an optimal dose in the diet may be an effective strategy to increase growth and resistance to bacterial disease with reduced mortality in shrimp farms.

• Journal of Ecology and Environment
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• 제45권1호
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• pp.24-39
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• 2021

Background: To address the lack of evidence supporting invasion by three invasive plant species (Imperata cylindrica, Lantana camara, and Chromolaena odorata) in tropical ecosystems, we compared the ecophysiological and leaf anatomical traits of these three invasive alien species with those of species native to Sempu Island, Indonesia. Data on four plant traits were obtained from the TRY Plant Trait Database, and leaf anatomical traits were measured using transverse leaf sections. Results: Two ecophysiological traits including specific leaf area (SLA) and seed dry weight showed significant association with plant invasion in the Sempu Island Nature Reserve. Invasive species showed higher SLA and lower seed dry weight than non-invasive species. Moreover, invasive species showed superior leaf anatomical traits including sclerenchymatous tissue thickness, vascular bundle area, chlorophyll content, and bundle sheath area. Principal component analysis (PCA) showed that leaf anatomical traits strongly influenced with cumulative variances (100% in grass and 88.92% in shrubs), where I. cylindrica and C. odorata outperformed non-invasive species in these traits. Conclusions: These data suggest that the traits studied are important for plant invasiveness since ecophysiological traits influence of light capture, plant growth, and reproduction while leaf anatomical traits affect herbivory, photosynthetic assimilate transport, and photosynthetic activity.

• Journal of Ecology and Environment
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• 제44권2호
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• pp.90-97
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• 2020

Background: Natural forests are generally considered to be less prone to biological invasions than other modified ecosystems, particularly when canopy cover is high. Few decades of management of degraded forests by local communities in Nepal has increased canopy cover and altered disturbance regimes. These changes might have reduced the abundance of invasive alien plant species (IAPS) in forests. To understand the status of IAPS in such forests, we studied two community managed Shorea robusta forests (Sundari and Dhusheri) of Nawalpur district in central Nepal. In these two forests, vegetation sampling was done using circular plots 10 m radius at forest edge, gaps, and within canopy. Variation of IAPS richness and cover across these microhabitats were compared, and their variation with tree canopy cover and basal area analyzed. Result: Altogether 14 IAPS were recorded in the study forests; among them Chromolaena odorata, Ageratum houstonianum, and Lantana camara had the highest frequency. Mikania micrantha was at the early stage of colonization in Sundari Community Forest (CF) but absent in Dhuseri CF. Both IAPS cover and richness was higher at forest edge and gap than in canopy plots and both these attributes declined with increasing canopy cover and tree basal area. Conclusion: The results indicate that increase in canopy cover and closure of forest gaps through participatory management of degraded forests can prevent plant invasions and suppress the growth of previously established IAPS in Shorea robusta forests of Nepal. This is the unacknowledged benefit of participatory forest management in Nepal.

• 한국자원식물학회지
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• 제32권5호
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• pp.543-558
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• 2019

2016년 환경부가 한반도 미유입 환경위해우려종으로 고시한 41분류군을 대상으로, 분류학적 특징을 이용하여 종을 동정하는 방법을 제공하고 또한 자생지의 분포 및 환경 특성에 대한 정보를 제공하기 위하여 본 연구를 수행하였다. 이를 위하여 국외의 여러 표본관에 소장된 건조표본을 확인하였으며, 미국 서부지역인 캘리포니아주, 오레곤주 및 워싱턴주의 현장조사를 수행하였다. 우선, 조사 결과의 일부로 미국 서부 농업지역을 중심으로 분포하는 Carduus acanthoides L. (국화과), Carduus tenuiflorus W. Curtis (국화과), Onopordum acanthium L. (국화과), Chromolaena odorata (L.) R.M. King & H. Rob. (국화과), Hydrocotyle ranunculoides L.f. (산형과), Oenanthe pimpinelloides L. (산형과), Ehrharta erecta Lam. (벼과), Paspalum conjugatum P.J. Bergius (벼과)의 8종에 대하여 위해성 검토, 잡초위험평가, 분류학적 특징 및 국내외 유사종과의 비교 검색표를 제시하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.65-65
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• 2018

외래종이라 함은 도입종, 귀화종, 침입종을 통칭하는 용어로 특정한 인위적 목적 여부에 관계없이, 본래의 자생지가 아닌 외부에서 들어와 다른 생물의 서식지를 점유하여 번식하는 생물종을 지칭하는 것으로, 침입외래종은 생태계에 도입되고 확산되면서 생물다양성과 연계된 생태계서비스를 위협하거나 또는 악영향을 미치는 외래종을 의미하며, 위해우려종이란 국내에 유입될 경우 생태계 등에 위해를 미칠 우려가 있어 환경부장관이 지정 고시하는 생물종 (살아있는 것으로서 개체의 일부 알 종자 등을 포함)을 지칭한다. 본 연구는 환경부고시에 따른 한반도 미유입 환경위해우려종43종에 대하여 농업환경위해성 평가를 실시하고, 분류동정 기법을 개발하는 등 기초 자료를 제공하고자 수행되었다. 이를 위해, 해외식물표본 확인과 미국 현장조사를 실시하였으며, 우선적으로 8종의 미유입 식물에 대하여 보고하고자 한다. - Carduus acanthoides L. (Asteraceae), Carduus tenuiflorus W. Curtis (Asteraceae), Onopordum acanthium L. (Asteraceae), Chromolaena odorata (L.) R.M. King & H. Rob. (Asteraceae), Hydrocotyle ranunculoides L.f. (Apiaceae), Oenanthe pimpinelloides L. (Apiaceae), Ehrharta erecta Lam. (Poaceae), Paspalum conjugatum P.J. Bergius (Poaceae).

• Journal of Ecology and Environment
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• 제47권3호
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• pp.75-84
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• 2023

Background: Knowledge of the spatial trends of plant invasions in different habitats is essential for a better understanding of the process of these invasions. We examined the variation in invasive alien plant species (IAS) richness and composition at two spatial scales defined by elevation and habitat types (roadside, forest, and cultivated lands) in the Makawanpur district of Nepal. Following an elevation gradient ranging from 500 to 2,400 m asl along a mountain road, plant species cover was recorded within sample plots of size 10 m × 5 m. Systematic random sampling was adopted in every 100 m elevation intervals on three habitat types. Results: Altogether 18 invasive alien plants belonging to eight families were recorded within 60 plots, of which 14 species (representing 80%) were from tropical North and South America. The most common plants by their frequency were Ageratina adenophora, Chromolaena odorata, Bidens pilosa, Lantana camara, and Parthenium hysterophorus. We found a significant relationship between species composition and elevation in the study area. Low-elevation regions had a higher number of alien species as compared to high-elevation regions within different habitat types. Conclusions: The species richness and density of IAS were higher in the road site followed by the cultivated land and forest sites. This pattern occurred throughout the elevation range and habitats. IAS were found mostly in the open land with high sunlight availability. Information from such scientific assessment of invasive alien plants will assist in developing appropriate management plans in the Makawanpur district.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권2호
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• pp.183-188
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• 2002

Anti-fungal potential of 5 plant extracts viz., Eucalyptus citriodora, Allium sativum, Cassia sophera, Chromolaena odorata and Datura metel on the growth of mulberry brown leaf spot pathogen Myrothecium roridum were examined. Except fur the aqueous extract of Allium bulb, ethanolic leaf extract of all other plants more efficiently reduced the colony growth of the fungus on potato-dextrose-agar, Of which, Allium and Eucalyptus extracts were more effective. Initiation of radial growth of M. roridum on solid media was deferred maximum 6 days by ethanolic Eucalyptus extract and 4 days by aqueous Allium extract at $0.4 mg.ml^{-1}$. In the liquid media amended with Eucalyptus extract ($0.4 mg.ml^{-1}$) complete inhibition of sporulation was noticed upto 8 days, and initial inhibition of mycelial bio-mass generation was considerably diminished with time and reduction was 1.3 fold 14 days after application. While, complete inhibition of mycelial growth for 6-14 days was recorded with $\geq$0.1 mg.ml$^{-1}$ commercial eucalyptus oil. However, rejuvenation of growth appeared when fungus was re-inoculated in fresh media. Post-inoculate application of different doses Of Eucalyptus and Allium extracts significantly (p < 0.05) reduced the disease severity in pot-ted mulberry. However, persistence of the effect up to 28 days was apparent at $\geq$ 1.0 mg.ml$^{-1}$ and effectively was on par with carbendazim (1 mg.ml$^{-1}$ ). Almost equal control ability of 1.0 mg.ml$^{-1}$ Eucalyptus extracts can be achieved by ca. 10 times lowered dose of commercial eucalyptus oil. It seems, the toxic principle of E. citrodora to M. roridum is fungistatic in nature and may have essential oil based origin.

• Journal of Ecology and Environment
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• 제48권2호
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• pp.182-195
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• 2024

Background: Invasive alien plant species (IAP) significantly threaten Nepal's protected areas and local communities. Understanding their distribution, impact, management, and utilization is essential for developing effective management strategies and sustainable utilization practices. The systematic literature review of publications from 2010 to 2023. The search was conducted through the database Nepal Journal online database (NepJOL) and Google Scholar, yielding an initial pool of 4,304 publication. After applying inclusion and exclusion criteria; we meticulously reviewed 43 articles for data extraction. Results: Seventeen IAP are found in protected area, Nepal with the highest prevalence observed in Koshi Tappu Wildlife Reserve, followed by Chitwan and Sukhlaphanta National Park. The most problematic species in terrestrial ecosystems are Mikania micrantha, Lantana camara, and Chromolaena odorata. The grassland ecosystems of wildlife habitats, primarily in the Terai and Siwalik regions, are the most invaded. Various management approaches are employed to mitigate the spread and impact of IAP, including mechanical methods such as uprooting, burning, and cutting. However, these methods are costly, and context-specific interventions are needed. The study also explores the potential use of IAP for economic, ecological, or cultural purposes, such as medicinal properties, energy production potential, and economic viability. Local communities utilize these plants for animal bedding, mulching, green manure, briquette, and charcoal production. Conclusions: Applying silvicultural practices alongside mechanical management is recommended to maintain a healthy terrestrial ecosystem and utilize the removed biomass for valuable products, thereby reducing removal costs and increasing income sources, potentially benefitting both local communities and wildlife in protected areas.