• Journal of Nutrition and Health
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• v.12 no.1
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• pp.31-41
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• 1979

Free amino acid in ethanol extracts and total amino acids in hydrolysates of eleven species of edible mushrooms were analyzed and determinated the contents five kind of new amino acid by means of amino acid autoanalyzer and gas liquid chromatography. The result obtained from this study are as follows. 1) Five kind of new amino acid turned out to be ${\alpha}$-aminobutyric acid, allo-isoleucine, ethanolamine, $\gamma$-aminobutyric acid and ornithine. 2) By means of amino acid autoanalyzer, the monoethanolamine was identified on the chromatogram ahead of alanine, ${\alpha}$-aminobutyric acid between peak of threonine and glycine, allo-isoeleucine between peak of valine and leucine, isoleucine, ${\gamma}$-aminobutyric acid followed by proline between peak of leucine, isoleucine and methionine and ornithine between peak of phenylalanine and tyrosine 3) By means of Gas liquid chromatography, the ${\alpha}$-aminobutyric acid was identified on the chromatogram between peaks of alanine and valine, allo-isoleucine between peaks of methionine and isoleucine, monoethanolamine followed by ${\gamma}$-aminobutyric acid between peaks of phenylalanine and ammonia, ornithine between the peaks of ammonia and lysine. 4) Of five amino acids which were identified, ornithine was the highest of its content in the mushroom extracts, and allo-isoleucine, ethanolamine, and ${\gamma}$-aminobutyric acid came next in decreasing order. 5) Also which were identified, ornithine was the highest of its content in the hydrolysates, and ${\alpha}$-aminobutyric acid, ${\gamma}$-aminobutyric acid, allo-isoleucine came next in decreasing order, ethanol extracts and hydrolysates of Auriculariaauricula-Judae(Fr.) $Qu\acute{e}l$ species didn't contain any of five kind of new amino acid. Ornithine also was the highest in the hydrolysates of ll mushrooms.

• Applied Biological Chemistry
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• v.35 no.4
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• pp.276-280
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• 1992

Quantutative analysis of catechins by HPLC was studied. When the mobile phase was switched from the conventional(AOAC) Methanol, Acetonitril and Acetic acid solution in $H_2O$ to 0.06% Phosphate solution with Acetonitrile, N,N-Dimethyl formamide, and Ethyl acetate, retention time could be reduced from 45 min to 28 min, especially, we obtained sharper chromatogram of the compounds, either (-)EGCG or (-)ECG, which resulted in minimization of analytical erros. CVs of retention time $(0.32{\sim}3.97%)$ and peak area $(1.61{\sim}7.01%)$ indicated that the data were more reliable. Content of catechins in commerical teas analyzed by the method was $120.3{\sim}153.7\;mg/g$ in green teas which was about 4 times that in black tea.

• Analytical Science and Technology
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• v.5 no.2
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• pp.159-167
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• 1992

An improved and accurate method for the determination of linear alkybenzene sulfonate(LAS) in river water is described by using gas chromatography and GC/MS. The gas chromatograph equipped with BP-5 capillary column was satisfactory for isolation of the homologues and isomers of LAS in river water. Four LAS homologues, $C_{10}$, $C_{11}$, $C_{12}$, $C_{13}$ alkyl chains were determined in Jungrang stream, Jinwi stream and down stream of Han river tributary. Especially $5-C_{11}$, $6-C_{11}$, $2-C_{12}$ and $6-C_{13}$, $7-C_{13}$ of LAS were identified by mass chromatogram. Different composition of LAS in standard and river water expressed the distribution and fate of LAS in river water. The remarkable reduced content of $C_{12}$ and $C_{13}$ in river water implied that the biodegradation of LAS more easily occured in longer alkylchain one. This method would be applicable for determination of LAS in water with high resolution and sensitivity.

• Korean Journal of Food Science and Technology
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• v.30 no.3
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• pp.480-486
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• 1998

It was investigated that the reduction of the intense brown color and increase of solubility, and depolymerization of polymerized melanoidins by ozonolysis affect their antioxidative and antimutagenic activities. Melanoidins was depolymerized and decolorized by ozonolysis. Ozone-treated melanoidins revealed a lower antioxidative activity and a higher antimutagenic activity than those of the untreated control. Fractions of ozone-treated melanoidins showed three peaks resemble to that of the melanoidins on Sephadex column chromatogram. Melanoidins of above MW 1,200 showed intense brown color. However, the strongest electron-donating ability was detected in the melanoidins of between MW 750 to 900. Ozone-treated and ozone-untreated melanoidins of between MW 900 to 1,000 revealed the higher antioxidative and antimutagenic activities.

• Journal of Ginseng Research
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• v.37 no.2
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• pp.248-253
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• 2013

Leaves from Panax ginseng Meyer (Korean origin and Chinese origin of Korean ginseng) and P. quinquefolius (American ginseng) were harvested in Haenam province, Korea, and were analyzed to investigate patterns in major metabolites using HPLC-based metabolic profiling. Partial least squares discriminant analysis (PLS-DA) was used to analyze the the HPLC chromatogram data. There was a clear separation between Panax species and/or origins from different countries in the PLS-DA score plots. The ginsenoside compounds of Rg1, Re, Rg2, Rb2, Rb3, and Rd in Korean leaves were higher than in Chinese and American ginseng leaves, and the Rb1 level in P. quinquefolius leaves was higher than in P. ginseng (Korean origin or Chinese origin). HPLC chromatogram data coupled with multivariate statistical analysis can be used to profile the metabolite content and undertake quality control of Panax products.

• Journal of the Korean Chemical Society
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• v.21 no.6
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• pp.445-448
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• 1977

This study was carried out to understand the activity of ${\alpha}$-chymotrypsin, a proteolytic enzyme, to a oligopeptide in the presence of various coloring food additives. 1. The melting point of synthetic oligopeptide, Asp-Arg-Val-Tyr-Ile-His-Pro-D-Ala, ((8-D-Ala) angiotensin Ⅱ) was 210∼$212^{\circ}C$. Chemical formula and molecular weight were $C_{44}H_{67}N_{13}O_{12}{\cdot}2CH_3COOH{\cdot}H_2O$ and 970.08, respectively. 2.The amino acid rations by acid hydrolysis were Asp : 1.01, Arg : 1.03, Val : 1.00, Tyr :40.94, Ile : 1.00, His : 1.05, Pro : 1.04, D-Ala : 1.03. 3. ${\alpha}$-Chymotrypsin cleaved the oligopeptide bond between tyrosine and isoleucine (Tyr-Ile). 4. The addition of food coloring additives as determined by paper chromatogram, did not influence the inhibitory activity of ${\alpha}$-chymotrypsin on oligopeptide, (8-D-Ala) angiotensin II.

• Journal of the Korean Chemical Society
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• v.8 no.2
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• pp.47-56
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• 1964

When a developed paper partition chromatogram was irradiated by means of the pneumatic tube system of the Korean research reactor (neutron flux: 1.5 ${\times}10^{12}n/cm^2$sec.) the qualitative confirmation of the developed spot on the chromatogram was possible. In the case of an organic halogen compounds (chloro-acid, chloro-ester, iodide, and fluoride) the spot analysis was possible by the present method whereas the same spot could not give the distinct coloring with a common coloring reagents. Filterpaper thickness calibration and activity calibration induced by irradiation of the components of the filter paper, which were a source of erraneous interpretation of the spot, were searched and an average filterpaper calibration method and filter paper activity were improvised to obtain a good qualitative analysis of the spot. Finally the use and applicability of this method for the analysis identification of an organic halogen compound were evaluated. As the filter paper phase an ordinary phase (Whatmann #1, filter paper) and reversed phase (liquid paraffin impregnated) were used.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.36 no.1
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• pp.89-92
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• 2010

In the previous study, we reported the antioxidative activity, antiaging activity, antibacterial activity and moisturizing effect of cream containing Persicaria hydropiper L. extract. In this study, the components of Persicaria hydropiper L. extract were analyzed by TLC and HPLC. Aglycone fractions obtained from the deglycosylation reaction of ethyl acetate fraction among the Persicaria hydropiper L. extract, showed 2 bands and 2 peaks in TLC and HPLC experiments, respectively. Two components were identified as quercetin and kaempferol. TLC chromatogram of ethyl acetate fraction of Persicaria hydropiper L. extract revealed 6 bands and HPLC chromatogram showed 7 peaks, which were identified as quercetin, hyperin, isoquercitrin, quercitrin, kaempferol. In conclusion, with the antioxidative activity, antiaging activity, antibacterial activity and moisturizing effect reported previously, component analysis of Persicaria hydropiper L. extracts could be applicable to new cosmeceuticals.

• Analytical Science and Technology
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• v.19 no.6
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• pp.512-518
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• 2006

The evidence containing some kind of petroleum at the fire spot was analyzed by gas chromatography to identify a fire's sources. To extract some petroleum from fire evidence, 10.0 mL of n-hexane was added in this solution, and it was shaken for 30 minutes. To identify a kind of petroleum in fire evidence, the prepared n-hexane solution was injected and analyzed in the gas chromatograph. The chromatogram of sample was different from those of thinner and gasoline that have low boiling point, and shown different peak pattern to heating and boiler oils. But it was similar to the chromatogram of diesel oil. After small amount of diesel oil was added to the sample, the area of characteristic peaks was increased more than those of raw sample. From the results, the kind of petroleum in the fire evidence was diesel oil.

• Analytical Science and Technology
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• v.21 no.6
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• pp.495-501
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• 2008

Agilent 5973 GC-MS instrument was modified so that the capillary direct interface was removed and that a silicone membrane was installed between GC and MS. Feasibility study of the membrane interface GC-MS has been carried out. Vacuum of the mass spectrometer was not affected by the carrier gas flow rate up to $4.7m{\ell}/min$. As the carrier flow rate was increased, peak tailing was reduced and chromatogram peaks appeared earlier. Chromatogram peaks showed better separation and higher sensitivity as the membrane thickness was reduced from $127{\mu}m$ to $75{\mu}m$, and also as the interface temperature was increased. However, the membrane interface GC-MS had drawbacks such as background ions at 73 and 147 m/z and poor peak separation due to peak tailing.