Browse > Article
http://dx.doi.org/10.5142/jgr.2013.37.248

HPLC-based metabolic profiling and quality control of leaves of different Panax species  

Yang, Seung-Ok (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Lee, Sang Won (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Young Ock (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Sohn, Sang-Hyun (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Kim, Young Chang (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Hyun, Dong Yoon (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Hong, Yoon Pyo (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Shin, Yu Su (Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Publication Information
Journal of Ginseng Research / v.37, no.2, 2013 , pp. 248-253 More about this Journal
Abstract
Leaves from Panax ginseng Meyer (Korean origin and Chinese origin of Korean ginseng) and P. quinquefolius (American ginseng) were harvested in Haenam province, Korea, and were analyzed to investigate patterns in major metabolites using HPLC-based metabolic profiling. Partial least squares discriminant analysis (PLS-DA) was used to analyze the the HPLC chromatogram data. There was a clear separation between Panax species and/or origins from different countries in the PLS-DA score plots. The ginsenoside compounds of Rg1, Re, Rg2, Rb2, Rb3, and Rd in Korean leaves were higher than in Chinese and American ginseng leaves, and the Rb1 level in P. quinquefolius leaves was higher than in P. ginseng (Korean origin or Chinese origin). HPLC chromatogram data coupled with multivariate statistical analysis can be used to profile the metabolite content and undertake quality control of Panax products.
Keywords
Panax leaves; Ginsenosides; HPLC; Metabolic profiling; Quality control;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Kim H, Chen X, Gillis CN. Ginsenosides protect pulmonary vascular endothelium against free radical-induced injury. Biochem Biophys Res Commun 1992;189:670-676.   DOI   ScienceOn
2 Kim HJ, Lee SG, Chae IG, Kim MJ, Im NK, Yu MH, Lee EJ, Lee IS. Antioxidant effects of fermented red ginseng extracts in streptozotocin-induced diabetic rats. J Ginseng Res 2011;35:129-137.   과학기술학회마을   DOI   ScienceOn
3 Lu JM, Yao Q, Chen C. Ginseng compounds: an update on their molecular mechanisms and medical applications. Curr Vasc Pharmacol 2009;7:293-302.   DOI   ScienceOn
4 Moco S, Vervoort J, Bino RJ, De Vos RC. Metabolomics technologies and metabolite identification. TrAC Trends Anal Chem 2007;26:855-866.   DOI   ScienceOn
5 Xiang YZ, Shang HC, Gao XM, Zhang BL. A comparison of the ancient use of ginseng in traditional Chinese medicine with modern pharmacological experiments and clinical trials. Phytother Res 2008;22:851-858.   DOI   ScienceOn
6 Wang A, Wang CZ, Wu JA, Osinski J, Yuan CS. Determination of major ginsenosides in Panax quinquefolius (American ginseng) using high-performance liquid chromatography. Phytochem Anal 2005;16:272-277.   DOI   ScienceOn
7 Block KI, Mead MN. Immune system effects of echinacea, ginseng, and astragalus: a review. Integr Cancer Ther 2003;2:247-267.   DOI   ScienceOn
8 Kim ND, Kang SY, Schini VB. Ginsenosides evoke endothelium-dependent vascular relaxation in rat aorta. Gen Pharmacol 1994;25:1071-1077.   DOI   ScienceOn
9 Carlini EA. Plants and the central nervous system. Pharmacol Biochem Behav 2003;75:501-512.   DOI   ScienceOn
10 Nah SY, Kim DH, Rhim H. Ginsenosides: are any of them candidates for drugs acting on the central nervous system? CNS Drug Rev 2007;13:381-404.
11 Attele AS, Zhou YP, Xie JT, Wu JA, Zhang L, Dey L, Pugh W, Rue PA, Polonsky KS, Yuan CS. Antidiabetic effects of Panax ginseng berry extract and the identification of an effective component. Diabetes 2002;51:1851-1858.   DOI   ScienceOn
12 Xie JT, Mchendale S, Yuan CS. Ginseng and diabetes. Am J Chin Med 2005;33:397-404.   DOI   ScienceOn
13 Yuan HD, Kim JT, Kim SH, Chung SH. Ginseng and diabetes: the evidences from in vitro, animal and human studies. J Ginseng Res 2012;36:27-39.   과학기술학회마을   DOI   ScienceOn
14 Yun TK. Experimental and epidemiological evidence on non-organ specific cancer preventive effect of Korean ginseng and identification of active compounds. Mutat Res 2003;523-524:63-74.   DOI   ScienceOn
15 Choi KT. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer. Acta Pharmacol Sin 2008;29:1109-1118.   DOI   ScienceOn
16 Lan K, Zhang Y, Yang J, Xu L. Simple quality assessment approach for herbal extracts using high performance liquid chromatography-UV based metabolomics platform. J Chromatogr A 2010;1217:1414-1418.   DOI   ScienceOn
17 Plank C, Lorbeer E. Quality control of vegetable oil methyl esters used as diesel fuel substitutes: quantitative determination of mono-, di-, and triglycerides by capillary GC. J High Resolut Chromatogr 1992;15:609-612.   DOI
18 Dunn WB, Ellis DI. Metabolomics: current analytical platforms and methodologies. TrAC Trends Anal Chem 2005;24:285-294.   DOI   ScienceOn
19 Qiu Y, Lu X, Pang T, Ma C, Li X, Xu G. Determination of radix ginseng volatile oils at different ages by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry. J Sep Sci 2008;31:3451-3457.   DOI   ScienceOn
20 Shin YS, Bang KH, In DS, Kim OT, Hyun DY, Ahn IO, Ku BC, Kim SW, Seong NS, Cha SW et al. Fingerprinting analysis of fresh ginseng roots of different ages using 1H-NMR spectroscopy and principal components analysis. Arch Pharm Res 2007;30:1625-1628.   과학기술학회마을   DOI   ScienceOn
21 Xie P, Chen S, Liang YZ, Wang X, Tian R, Upton R. Chromatographic fingerprint analysis-a rational approach for quality assessment of traditional Chinese herbal medicine. J Chromatogr A 2006;1112:171-180.   DOI   ScienceOn
22 Beyer T, Diehl B, Randel G, Humpfer E, Schafer H, Spraul M, Schollmayer C, Holzgrabe U. Quality assessment of unfractionated heparin using 1H nuclear magnetic resonance spectroscopy. J Pharm Biomed Anal 2008;48:13-19.   DOI   ScienceOn
23 Ligor T, Ludwiczuk A, Wolski T, Buszewski B. Isolation and determination of ginsenosides in American ginseng leaves and root extracts by LC-MS. Anal Bioanal Chem 2005;383:1098-1105.   DOI   ScienceOn
24 Shi W, Wang Y, Li J, Zhang H, Ding L. Investigation of ginsenosides in different parts and ages of Panax ginseng. Food Chem 2007;102:664-668.   DOI   ScienceOn
25 Eriksson L, Johansson E, Kettaneh-Wold N, Wold S. Multi-and megavariate data analysis. Umea: Umetrics AB, 2006.
26 Okada T, Afendi FM, Altaf-Ul-Amin M, Takahashi H, Nakamura K, Kanaya S. Metabolomics of medicinal plants: the importance of multivariate analysis of analytical chemistry data. Curr Comput Aided Drug Des 2010;6:179-196.   DOI   ScienceOn