• Preventive Nutrition and Food Science
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• v.18 no.3
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• pp.157-162
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• 2013

The purpose of this study was to investigate the effects of the ethanol extract of Cynanchum wilfordii (ECW) on the blood lipid profile of hypercholesterolemic rats. Thirty 7-week-old male Sprague-Dawley rats were allowed free access to either a normal diet (AIN-93 diet), or 1% high-cholesterol diet with or without 0.5% or 1% ECW for 5 weeks. After sacrifice, the rat serum lipid profile was analyzed. The diets containing ECW decreased body weight gains compared to the normal diet. Serum HDL-cholesterol levels of ECW-fed groups were significantly increased in the hypercholesterolemic groups and normal groups (P<0.05). When 1% ECW was fed to the normal group, total cholesterol level was increased. Moreover, treatment of ECW in hypercholesterolemic groups yielded a dose-dependent and highly significant decrease in the atherogenic index as compared to the control. These results suggest that intake of Cynanchum wilfordii may help reduce the risks of hypercholesterolemia by increasing blood HDL-cholesterol and lowering the atherogenic index.

• Nutrition Research and Practice
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• v.3 no.1
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• pp.31-37
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• 2009

Recently, Korean people are consuming seaweeds almost 3.5 times more now than three decades ago. It is well known that seaweeds contain lots of soluble dietary fiber in addition to micronutrients such as ${\beta}$-carotene, iodine and some bioactive components. Seaweeds are considered to be effective for preventing chronic diseases including obesity, diabetes mellitus, atherosclerosis, cancer or constipation. This study was conducted to investigate the effect of seamustard intake on body weight gain, blood glucose level and lipid profiles in rats fed diets with different energy nutrient composition. Male Sprague-Dawley rats (average initial weight 103.7g) were divided into groups for two experiments as follows; Control, M2.5 & M5 groups (Exp. I) and M5, M10, HCM5, HCM10, HFM5 & HFM10 groups (Exp. II). The rats were fed diet and water ad libitum for 4 weeks. In general, there was no significant difference in blood glucose and triglyceride concentration among groups. In Exp. I, serum LDL-cholesterol level of rats fed diet with 5% seamustard powder (M5) was significantly lower than that of control group, while HDL-cholesterol level, TC/LDL ratio and weight of adrenal gland were higher. In Exp. II, food intake, body weight gain and EER of high fat diet with 10% seamustard group (HFM10) were the lowest among groups. Except gastrocnemius muscle, all organ weights of HFM10 group were the lowest. Fecal cholesterol excretion and serum LDL-cholesterol concentration of HFM10 group were the highest, while serum HDL-cholesterol level was the lowest among groups. Interestingly, HDL-cholesterol concentration was the highest in HCM5 group among groups. From these results, it was suggested that seamustard intake might be more effective for body weight control, but not for improving blood lipid profiles in high fat diet than in high carbohydrate diet.

• Journal of Nutrition and Health
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• v.23 no.1
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• pp.1-10
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• 1990

This study was designed to investigate the effect of supplementation of fish oil on serum lipid in young healthy females. Eighteen female college students were divided into 3 groups. Each group fed a typical Korean diet supplemented with 15g, 12g and 9g of fish oil respectively for 1 week. Blood samples were obtained 4 times before supplementation, immediatly after supplementation, 1 week after stopping supplementation and 3 week after stopping supplementation. After 6 week break, the doses of fish oil were interchanged among 3 groups and the experiment was repeated to reduce interindivisual variation. The level of total cholesterol(TC), triglyceride(TG), lipoprotein, high density lipoprotein(HDL)-cholesterol, low density lipoprotein(LDL)-cholesterol in the serum samples were determined immediately after stopping supplementation and then the value compared with those of the before supplementation period. The results obtained are summarised as follows; The serum TC levels decreased significatly(p<0.01) immediately after supplementation of fish oil and 1 week after stopping supplementation. The serum TG levels also decreased significantly(p<0.05) immediately after supplementation of fish oil. The HDL-cholesterol levels increased significantly(p<0.05) at 2 week and 3 weeks after stopping supplementation of fish oil in the 12g and the 15g supplementation groups. The LDL-cholesterol levels decreased significantly(p<0.05) immediately after and 1 week after stopping supplementation of fish oil. The ratio of HDL-C to TC increased significantly(p<0.01) and the ratio of LDL-C to HDL-C, and index of artherogenesity, decreased significantly(p<0.05).

• Journal of Preventive Medicine and Public Health
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• v.39 no.6
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• pp.505-510
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• 2006

Objectives : The purpose of this study is to evaluate the association of the angiotensin converting enzyme (ACE) insertion/deletion (I/D) polymorphism with cardiovascular disease risk factors. Methods : Out of a total of 608 middle-aged adults who visited local health centers, 424 subjects (104 male, 320 female) who had not been diagnosed with hypertension, diabetes mellitus, or hyperlipidemia were included in this study. ACE genotypes were determined in all subjects by polymerase chain reaction methods. Results : Statistical differences in high-density lipoprotein (HDL) cholesterol levels according to ACE genotype were observed using ANOVA (p<0.05), but no differences were found in other cardiovascular risk factors. Specifically, men with the DD and DI genotypes had significantly lower HDL cholesterol levels than those with the 11 genotype based on the LSD multi-comparison test (p<0.05). Conclusions : In men, the D-allele of the ACE I/D polymorphism was significantly associated with reduced HDL cholesterol levels. In the future, larger studies are needed to confirm this relationship between ACE I/D polymorphism and HDL cholesterol.

• Journal of Physiology & Pathology in Korean Medicine
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• v.16 no.1
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• pp.89-94
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• 2002

Effect of Gamisamul-tang(GS) on body weight, uterine weight, splenial weight, serum and estrogen receptor-α (ER-α) in kidney were investigated in ovariectomized(OVX) rats. Female Sprague -Dawley rats with a body weight of 220 g were undergone ovariectomy or sham operation. The ovariectomy caused significant decrease in serum levels of HDL-cholesterol and in uterine weight compared to those of sham-operated animals. while caused significant increase in serum levels of total cholesterol, LDL-cholesterol, triglyceride, body weight and in splenial weight. In contrast, administration of OVX rats with the GS significantly elevated serum levels of HDL-cholesterol and uterine weight, but significantly depressed serum levels of total cholesterol, LDL-cholesterol, triglyceride, body weight and in splenial weight. ER-α localized in the kidney vessel endothelial cells and its expression was increased by ovariectomy and decreased by administration of OVX rats with the GS. Our results suggest that a possible protective effect of as against hyperlipidemia in postmenopausal cardiovascular disease and as increase the estrogen-estrogen receptor romp/ex in the kidney vessel endothelial cells.

• The Korea Journal of Herbology
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• v.22 no.3
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• pp.109-116
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• 2007

Objective : In order to elucidate hyperlipidemic effects of the fruits of Poncirus trifoliata and Citrus aurantium, our experimental study was performed on hyperlipidemia rats induced by Triton WR-1339. Method : The hyperlipidemia rats induced by intraperitonial injection of triton WR-1339 were treated with the fruits of P. trifoliata (rare and preparata) and C. aurantium 50 and 200mg/kg. The amount of triglyceride. total cholesterol. HDL-cholesterol, LDL-cholesterol, AST. and ALT levels were measured. Results: The levels of triglyceride. total cholesterol and LDL-cholesterol were significantly reduced in fruits of P. trifoliata (rare and preparata) and C. aurantiu. Simvastatine treated group compared with those of control but the level of HDL-cholesterol was incresed. The level of AST and ALT were not significantly reduced by the treatment to the fruits of P. trifoliata (rare and preparata) and C. aurantium. Conclusion : In the hyperlipidemia rats induced by triton WR-1339. the fruits of P. trifoliata (rare and preparata) and C. aurantium showed a hyperlipidemic effect.

• The Journal of Internal Korean Medicine
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• v.35 no.3
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• pp.244-261
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• 2014

Objectives: This experiments were performed to determine the effects of the Jehasuogamibang (製何首烏加味方: JHGB) on antioxidationactivity and hyperlipidemia induced by a hypercholesterolemic diet in mice. Methods: After treatment with JHGB expert safety in cytotoxicity and toxicity of Human fibroblast cells and liver and kidney, effect on Reactive Oxygen Species, serum total cholesterol, LDL-cholesterol, triglyceride, glucose, HDL-cholesterol, lipid peroxid of liver tissue, significantly increased SOD and catalase. Results: 1. JHGB showed safety in cytotoxicity and toxicity of human fibroblast cells and liver and kidney. 2. JHGB showed significant inhibitory effect on reactive oxygen species. 3. JHGB significantly decreased serum total cholesterol, LDL-cholesterol, triglyceride, and glucose, and significantly increased serum HDL-cholesterol. 4. JHGB significantly decreased lipid peroxide of liver tissue and significantly increased SOD and catalase. Conclusions: These results suggest that Jehasuogamibang is effective in antioxidationactivity and dietary hyperlipidemia-induced mice.

• Journal of Pharmaceutical Investigation
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• v.24 no.4
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• pp.297-300
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• 1994

Development of hyperlipidemic animal model is prerequisite for the screening of drugs lowering the blood lipoprotein level. In this study, two kinds of diet regimens were tried for the hyperlipidemic effect. The model will be used for the screening of current prescriptions to a variety of diseases related to hyperlipidemia by practioner of traditional herbal medicine. Fifteen male Wister rats of 200g were divided into three groups, and given normal, or either one of the two high cholesterol diets for up to 2 weeks. Regimen HC-1 and regimen HC-2 contained I% and 2% cholesterol in diet, respectively. Total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL) and lower density lipoprotein cholesterol (LDL) levels in the serum were measured before the start of the diet, at 1 week after the start, and at 2 week point. Both regimens resulted in hyperlipidemia with the typical characteristics of increase in TC and LDL, and reduction in HDL, and showed no observable side effects such as diarrhea. In conclusion, both of HC-1 and HC-2 regimens appeared appropriate as diet regimens for the hyperlipidemic rat model.

• Korean Journal of Community Nutrition
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• v.6 no.1
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• pp.69-75
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• 2001

Intake of soy protein the fisk factors associated with cardiovascular disease in postmenopausal women. This study was designed to effects of isoflavone supplementation on serum lipids in 16 hyperlipidemic postmenopausal women . For this purpose, an intervention study was conducted for 12 weeks. Subjects were healthy, free-living women consuming habitual diets with 0.3ｇ/ｄ of isoflavone. Food and nutrient intake was obtained by 24-hr recall method and anthropometric measurement were made. Systolic and diastolic blood pressure, total serum cholesterol. HDL-cholesterol and LDL-cholesterol were determined before and after the isoflavone supplementation. The results were summarized as follows. The average age, hight, weight and BMI of the subject were 65.3 years, 151.4 cm, 62.2 kg and 27.1, respectively. The systolic blood pressure and diastolic blood pressure were not reduced significantly with isoflavone supplementation. Total cholesterol (p＜0.001), HDL-C(p＜0.05), and LDL-C(p＜0.01) were significantly increased after isoflavone concentration. In conclusion, isoflavone supplementation was not effective to modify risk factors for cardiovascular disease.

• Journal of Pharmacopuncture
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• v.10 no.1 s.22
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• pp.109-119
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• 2007

In order to study the effects of Pinus densiflora on hyperlipidemia and lipid in rats, we divided the rats into groups(Normal group, Control group and Sample group) and perfomed the experimental research. Hyperlipidemia and lipid in rats were induced by high fat diets for 8weeks. The sample group was administerd the extract of Pinus densiflora for 14 days and control group was administerd equal dose of oral. And then we measured the amount of serum triglyceride, Total cholesterol, LDL-cholesterol, HDL-cholesterol, Free Fatty Acid, phospholipid, Insuline, Laptin, Body weight, epididymis fat weight & rate, epididymis fat cell, Cardiac Risk Factor(CRF). The results were as followers : 1. Sample Group showed decreasing effects on Total cholesterol, Trigylceride, LDL-cholesterol, and Phospholipid levels in serum and CRF significantly. 2. Sample Group showed increasing effects on HDL-cholesterol level in serum significantly. 3. Sample Group showed decreasing effects on Insuline in serum significantly. 4. Sample Group showed increasing effects on Laptin in serum significantly. 5. Sample Group showed decreasing effects on Body weight, epididymis fat weight & rate, epididymis fat cell significantly. According to the above results, Pinus densiflora showed significant decreasing effects on hyperlipidemia and lipid in rats, it is considered that it is appropriate to apply for hyperlipidemia, obesity.