• Journal of the Korean Wood Science and Technology
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• 제48권6호
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• pp.888-895
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• 2020

Ulmus genus has excellent various physiological activities, including anti-ulcer, antioxidant, antibacterial, anti-cancer, immunity, and homeostasis maintenance effects, and it is known to have many additional drug effects And one of reasons for these excellentbiological activities is a flavan-3-ol chemical group in Ulmus genus. In this study a new flavan-3-ol compound was identified in Ulmus davidiana var. japonica. A flavan-3-ol,(2R,3S)-7-[(2S,3R,4R)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-2-(3,4-dihydroxyphenyl)-3,4-dihydro-2H-chromene-3,5-diol, named as catechin 7-O-beta-D apiofuranoside, was isolated from the stems and barks of Ulmus davidiana var. japonica for. suberosa, which is a species belonging to the genus Ulmus, growing throughout the Korea peninsula. The structure was elucidated by various spectroscopic methods including high-resolution TOF mass spectrometry, ¹H-NMR and ¹³C-NMR spectrometry and comparison with chemical structures of defined compounds.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.204-208
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• 2002

A Streptomyces sp. isolated from a soil sample collected in Taejeon, Korea has previously been found to produce two new polyene antibiotics. The two new antibiotics were named "16-methyloxazolomycin (antibacterial)" and "tetrin C (antifungal)", and their chemical structures are presented elsewhere [10, 11]. In the current study, chemotaxonomy, numerical taxonomy, and ISP methods were all employed for the taxonomic study. The spore chains were spirales and the spore surface was smooth. The spore mass was a gray series and no melanin pigment was produced. On the basis of the morphological and physiological properties, the microorganism was identified to be Streptomyces erumpens, belonging to the gray series of category IV, as defined by Bergey′s Manual. Tetrin C at the concentration of 20 ${\mu}g$/ml demonstrated a potent in vivo (pot test) preventive effect against rice blast, rice sheath blight, cucumber gray mold, wheat powdery mildew, and barley leaf rust.

• 생약학회지
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• 제27권2호
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• pp.129-135
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• 1996

Euphorbia ebracteolata Hayata is a perennial herb of Euphorbiaceae that has a cyathumal inflorescence, the glands of the involucres are rounded-cordate and reddish, stems are erect, thick and 40-50 cm high. The leaves are alternate and rather numerous. So far this plant has been known as a unreported species in Korea. However, as the result of this study, this plant was found to be widely distributed in Korea over eight habitats, i.e. Keunduk, Dokye and Kaliwangsan, Kangwon-do: Dunduksan, Kyongsangbuk-do: Okcheon, Chungcheongbuk-do: Manduksan, Baekyangsan and Naejangsan, Jeolanam-do. On the other hand, its variety, E. ebracteolata var. coreana Hurusawa which grows only in Poong-do, Kyongki-do was reviewed by comparative morphology and chemotaxonomy to be resulted in the same species, E. ebracteolata.

• 대한화학회지
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• 제51권1호
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• pp.51-55
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• 2007

화탄과정의 폐수에서 단리된 티오시안산염 이용 균주인 Bacillus brevis의 지방산 조성을 기체크로마토그라피로 분석하였다. 이 균주는 포화 및 불포화 지방산이외에 히드록시 지방산을 포함하고 있다. 히드록시 지방산은 일반적으로 흥미로운 박테리아의 화학적 계통분리학의 지표로 알려져 있다. 이 균주에는 시클로프로판 지방산이 전혀 없다. 이균주와 Baciluus brevi B-33 및 B-34 균주의 지방산 조성을 비교하였을 때 이 균주들 사이에 차이가 있었다. 이러한 차이는 티오시안산염의 충격 효과 때문일 것으로 보인다. 이 결과는 지방산의 생합성이 환경에 크게 의존된다는 것을 지지하여 준다.

• Archives of Pharmacal Research
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• 제27권12호
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• pp.1270-1274
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• 2004

Random amplified polymorphic DNA (RAPD) analysis was used to determine the genetic relationships among seventeen species of the Acanthopanax species. The DNA isolated from the leaves of the samples was used as template in polymerase chain reaction (PCR) with twenty random decamer primers in order to distinguish plant subspecies at the level of their genomes. The RAPD patterns were compared by calculating pairwise distances using Dice similarity index, and produced to the genetic similarity dendrogram by unweighted pair-group method arithmetic averaged (UPGMA) analysis, showing three groups; a major cluster(twelve species), minor cluster (4 species) and single-clustering species. The results of RAPD were compatible with the morphological classification, as well as the chemotaxonomic classification of the Acanthopanax species. The Acanthopanax species containing 3,4-seco-lupane type triterpene compounds in their leaves corresponded to the major cluster, another species having oleanane or normal lupane type constituents to minor clusters, and one species not containing triterpenoidal compound to single-cluster.

• 한국식품영양과학회지
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• 제21권6호
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• pp.681-685
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• 1992

돼지고기에서 분리한 bacteriocin 생성능이 우수한 젖산균을 동정하기 위하여 이미 알려진 몇가지 젖산균을 참고균으로 하여 10% denaturing polyacrylamide gel에 전기영동으로 전개한 5S rRNA 와 tRNA 등 150개 이하의 핵산으로 구성된 저분자량 RNA(Low Molecular Weight RNA : LMW RNA) profiles에 나타난 15~25개의 밴드 양상이 균에 따라 차이점을 보여 동정에 효과적으로 이용할 수 있었다. 새로 분리한 젖산균은 참고균과는 다른 균종이었다. APT, TSB, MRS의 3종류 다른 배지에서 배양하여도 LMW RNA profiles에는 차이가 없었으며, 겔상의 밴드 전개거리를 3개의 표준 분자량 물질을 이용하여 만든 표준곡선을 사용하여 상대핵산단위(relative nucleotide units : RNU)로 나타낸 밴드의 양상을 도표화 하는 것이 profiles를 서로 비교 하는데 편리하였다.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1265-1274
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• 2015

Secondary metabolite-based chemotaxonomic classification of Streptomyces (8 species, 14 strains) was performed using ultraperformance liquid chromatography-quadrupole-time-offlight-mass spectrometry with multivariate statistical analysis. Most strains were generally well separated by grouping under each species. In particular, S. rimosus was discriminated from the remaining sevens pecies (S. coelicolor, S. griseus, S. indigoferus, S. peucetius, S. rubrolavendulae, S. scabiei, and S. virginiae) in partial least squares discriminant analysis, and oxytetracycline and rimocidin were identified as S. rimosus-specific metabolites. S. rimosus also showed high antibacterial activity against Xanthomonas oryzae pv. oryzae, the pathogen responsible for rice bacterial blight. This study demonstrated that metabolite-based chemotaxonomic classification is an effective tool for distinguishing Streptomyces spp. and for determining their species-specific metabolites.

• 생약학회지
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• 제24권4호
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• pp.282-288
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• 1993

Very little utilization of the fruits of Malus baccata(Rosaceae) has been employed for food and medicinal plants except for preparing fruit beverages. But, it was estimated as valuable to investigate the chemical components for the botanical resource of this plant. In this study, it was found that the fruits of this plant contained primary long chain alcohol, ${\beta}-sitosterol$, campesterol, ursolic acid and ${\beta}-_D-glucosides$ of ${\beta}-sitosterol$ and campesterol. However, phloretin(dihydrochalcone) and its 5-O-glucoside(phloridzin) known as plant growth regulators in many Rosaceae plants were not found in this plant material by co-TLC analysis with authentic specimens. Although plant sex hormone, estrone, was often contained in relates of M. baccata, e.g., Prunus spp., Crataegus spp. and Malus spp., this compound was not detected in this fruit by comparison with an authentic material. By RIC chromatography, it was suggested that the Soxhlet extraction by the solvent of ether was excellently useful to extract ursolic acid efficiently.

• Mycobiology
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• 제50권2호
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• pp.110-120
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• 2022

The goal of the present study was to investigate the antibacterial properties, enzyme production, and metabolic profiling of a new Ceratorhiza hydrophila strain isolated from the submerged aquatic plant Myriophyllum spicatum. Furthermore, the fungus' morphological characterization and DNA sequencing have been described. The fungus has been identified and submitted to the GenBank as Ceratorhiza hydrophila isolate EG19 and the fungus ID is MK387081. The enzyme analyses showed its ability to produce protease and cellulase enzymes. According to the CSLI standard, the ethyl acetate extract of C. hydrophila showed intermediate antibacterial activity against Streptococcus pneumonia, Micrococcus luteus, and Staphylococcus aureus. Metabolic profiling has been carried out using 700 MHz NMR spectroscopy. Based on the ¹H and ¹H-¹³C heteronuclear single quantum coherence (HSQC) NMR data and NMR databases, 23 compounds have been identified. The identified metabolites include 31% amino acids, 9% sugars, 9% amines, 4% sugar alcohols, and 4% alkaloids. This is the first report for the metabolic characterization of C. hydrophila, which gave preliminary information about the fungus. It is expected that our findings not only will pave the way to other perspectives in enormous applications using C. hydrophila as a new promising source of antimicrobial agents and essential metabolites, but also it will be valuable in the classification and chemotaxonomy of the species.

• Journal of Forest and Environmental Science
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• 제40권1호
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• pp.53-63
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• 2024

Alnus japonica is indigenous to korea and usually grows in wet lowlands and damp regions in the mountains. Oregonin, is known as a representative compound of the diarylheptanoid mainly found in Alnus species and has been reported to have anti-inflammatory and antioxidant effects. In this experiment, we conducted a study on the presence of orogonin in the native Alnus japonica from the Baekdudaegan mountain range in Korea. We collected a total of 30 samples from Chuncheon, Yangyang, and Jeongseon and conducted experiments. In the HPLC analysis, we confirmed that oregonin was detected in all samples. After the quantitative analysis, samples with the highest content from each region were selected and LC-MS/MS experiments were performed. As a result, the same molecular weight as oregonin, 477.2 m/z, was confirmed in all samples.