• 한국식품위생안전성학회지
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• 제19권4호
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• pp.198-202
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• 2004

잔류물질 간이 시험법으로 EEC-4 plate test와 Charm II test가 항생물질과 합성항균제 잔류검사에 널리 이용되고 있는 바, 이들 상호간의 결과 수치를 비교, 분석한 결과 다음과 같은 결론을 얻었다. EEC-4 plate혹은 Charm II 에서 양성으로 나타난 돈육 71건에 대하여 HPLC로 정량 분석한 결과, only tetracyclines 46건, only sulfonamides와 solfonamides+tetracyclines가 각각 4건씩, amphenicols+tetracyclines와 amphenicols+sulfonamides+tetracyclines가 각각 1건씩 검출되었고, 15건에서는 검출되지 않았다. Sulfonamides 계열에서 검출된 9건의 경우, Charm-II test 결과 수치가 낮은 경우에 정량 검출량이 높게 나타났고, EEC-4 plate test 결과 pH 6.0, pH 7.2, pH 8.0 모두에서 양성으로 나타나는 등 두 시험방법 간에 연관성이 있었고, chloramphenicols 계열에서 검출된 2건과 검출되지 않은 7건의 경우, Charm-II test결과 수치가 낮은 경우에는 오히려 정량 검출되지 않았으나, control point 근방의 양성 수치에서는 검출량이 높게 나타날 뿐 아니라 EEC-4 plate test에서도 pH6.0, pH7.2, pH8.0 모두에서 양성으로 나타나는 등 두 시험방법 간에 연관성이 있었다. 한편, tetracycline계열의 경우, EEC-4 plate와 Cham-II간에 연관성은 없었다. 또한, EEC-4 plate와 Charm II에서 각각 양성을 나타내었으나 HPLC에서는 검출되지 않는 경우는 tetracyclines계열에서는 $10.5\%(6/57)$, sulfonamides계열의 경우는 없었고, chloramphenicols계열의 경우는 $81.8\%(9/11)$로 각각 나타나 EEC-4 plate와 Charm II가 세 가지 잔류물질 시험에 대한 screening method로서 적합함을 알 수 있었다.

• 한국식품위생안전성학회지
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• 제11권4호
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• pp.307-314
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• 1996

The European Economic Community four plate test(EEC 4-plate test, FPT, EU) has been used for monitoring antimicrobial drug residues in meat by Local Veterinary Service Center in Korea. This study was performed to evaluate sensitivity and group specificity of some antimicrobial drugs in FPT and to compare FPT with Charm II test. The minimal detectable levels of targeted antimicrobial drugs tested with standard solutions were 0.025∼1.0 ppm for 7 beta-lactams, 0.5∼1.0 ppm for 4 aminoglycosides, 0.05∼0.5 ppm for 5 macrolides, 0.05∼0.25 ppm for 3 tetracyclines and 0.25&1.0 ppm for 6 sulfonamides. In comparison of FPT and Charm II test, the results of FPT were not accord with those of Charm II test having the group specificity of seven families of antimicrobial drugs in meat samples except some families like tetracyclines.

• 한국동물위생학회지
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• 제25권3호
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• pp.229-236
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• 2002

This study was carried out to compare the residual antibiotic materials in muscles of slaughter cattle and swine from slaughterhouses in Seoul from 2000 to 2001 by EEC-4-plate method, Charm II and HPLC method. 1. Residual antibiotic materials were detected from 95 samples(0.8%) by EEC-4-plate and 57 samples(10.2%) by Charm II. The final HPLC method determined the positives are 43(45.3%) and 27(47.3%) respectively. 2. The detection ratios were 45% by EEC-4-plate and 47% by Charm II. 3. Seventy samples were classified as tetracyclines 56(75.7.4%), sulfonamides 10(14.9%), $\beta$-lactam 6(8.1%) chloramphenicol 1(1.4%). Three of them were confirmed to be positive simmultaneously for tetracyclines, sulfonamides and chloramphenicol. 4. The highest residual concentration of chlortetracycline, oxytetracycline, sulfamethazine, sulfadimethoxine, sulfaquinoxaline, penicillin, ampicillin and chloramphenicol were 0.34, 11.29, 68.16, 0.13, 4.0, 0.12, 0.4 and 0.04ppm, respectively.

• 한국토양비료학회지
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• 제47권2호
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• pp.133-139
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• 2014

Veterinary antibiotics (VAs) in animal compost and organic fertilizer can have adverse effect on ecosystem and eventually human health. The main purpose of this research was to evaluate feasibility of Charm II system for monitoring residuals of VAs in animal compost and organic fertilizer. Four different VAs (Tetracyclines: TCs, Sulfonamides: SAs, Macrolides: MLs, and ${\beta}$-lactams: ${\beta}$-LTs) were analyzed and total of 100 samples were monitored. Results reveled that SAs in animal compost showed the highest detection frequency (64%) with exceeded concentration of criteria. However, very low detection frequency (0-12%) for ${\beta}$-LTs was observed in animal compost and organic fertilizer. Depending on physicochemical properties of each VAs, detection frequency of VAs was determined. In conclusion, charm II system can be utilized to screen if residual of VAs is in animal compost and organic fertilizer. Also, more research is necessary to establish standard method for analysis of VAs in complex matrix and to minimize adverse effect of VAs from source to environment.

• 한국식품위생안전성학회지
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• 제11권2호
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• pp.129-132
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• 1996

Recently, as concern about the residual antibiotics in milk increase, the detection methods of residual antibiotics used extensevely at the present time were investigated and compared to their properties and the detection limits of variable antibiotics. At first, comparactive tests of the detectable sensitivity of 4 test organisms, B. cereus, B. subtilis, M.luteus, B.stearothermophilus C-953, were performed by disc assay. As a result, B.stearothermophilus was the most sensitive strain of all other strains and showe the detect limit of 5-50 ppb for penlicillins (PCs). And also, B.subitilis was showed the more effective detection limit, 200-400 ppb, for aminoglycosides (AGs) and M.luteus was showed predominant sensitivity , 50-500 ppb for macrolides(MLs) and B.cereus was the most sensitive strain for tetracyclines (TCs) and showed the detection limit of 100-400 ppb. Therefore, each test strains were showed a different sensitivity in the detection of the different antibiotic families. When the detection limit of disc assay and other methods were compared, TTCmethod was less sensitive than other methods showing 5-50 ppb detectable lebel for PCs. Also, for the detection of other antibiotic families TTC method was showed the worst sensitivity and Delvo and Charm Farm tests were similar to the detectable properties of AGs and MLs. Although disc assay was showed the similar detection limit for PCs with Delvo and Charm Farm, it was more widely effective for the detection of kanamycin, erythromycin, chlortetracycline, doxycycline, verginiamycin and so on than Delvo or Charm Farm. CharmII test was showed the best sensitivity for the most of antibiotics except neomycin and gentamycin. But it was necessary that different tests must be performed to each antibiotic family and so it was regarded that the effectiveness of that method was low.

• 한국의류학회지
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• 제30권5호
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• pp.684-698
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• 2006

This study consists of the stimuli of a female model in her twenties with twenty-two different facial make-up. The subjects of this study are one thousand low hundred ninety seven purposive sampled-male and female grown-ups throughout the country. The period of the research was the December of 2004, one month, and the materials were analyzed by factor analysis, T-examination, analysis of variance, Cronbach's a, Duncan's Multiple Range Test. Here follows the result of the research. Firstly, Familiarity, Intelligence, Fitness, Charm, Tradition and Youth were came out as the result of factor analysis of make-up color image perception. Secondly, in age/lip color perception of bright skin tone, there was difference of Intelligence and Charm. In age/image make-up perception of bright skin tone, there was difference of Familiarity, Charm especially on Cool image make-up. Thirdly in habitant/lip color perception of dark skin tone, there was difference of Intelligence and Charm. In habitant/image make-up perception of bright skin tone, there was difference of Familiarity, Charm and of bright skin tone, Intelligence, Charm, Tradition and Youth. Fourthly, there were the interaction effects on the gender of perceivers and lip color and image make-up of perceivers habitant. Lastly, in preference rate, lip color was more affected by age and image make-up were more affected by perceivers habitant.

• 한국동물위생학회지
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• 제21권1호
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• pp.13-20
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• 1998

This survey was carried out to detect the residual antibiotics and sulfonamides in emergency slaughtered cattle(n=265) from slaughter houses in Kyeonggi province by EEC 4-plates method, Charm II and HPLC. The results were summarized as follows ; 1. Antimicrobial substances were detected from 24 samples(9.1%) by EEC 4-plates method and the detection ratio were highest in summer(13.8%). 2. Twenty-two of the 24 positive samples were classified as sulfonamide(34.4%), TCs(31.3%), $\beta$-lactam(23.5%) and aminoglycoside(9.3%) by Charm II test. 13(59.1%) of the 22 samples contained single agent and 9 samples(40.9%) contained 2 or more agents. 3. Oxytetracycline(27.3%), penicillin G (27.3%) and sulfathiazole(18.2%) were detected in 20 from 22 samples by the HPLC and Charm II test. 4. The residual concentration of oxytetracycline, penicillin G and sulfonamide were 0.29~9.30 ppm, 0.05~9.58 ppm and 0.04~7.59 ppm, respectively and 19 samples(7.2%) were exceeded tolerance levels.

• 한국동물위생학회지
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• 제29권3호
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• pp.331-338
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• 2006

This survey was carried out to detect the residual antibiotics in beef (n = 1,071), pork (n=7,837) and chicken (n=1,536) from slaughter houses in Gyeongbuk province by EEC-4 plate method, Charm II and HPLC during 2005. Residues of antibiotic were detected from 9 beef (0.8%) and 119pork (1.52%) by EEC-4 plate method, and total positive rates were 1.23% (128). 126 samples of the 128 positive samples by the EEC-4 plate method were detected by charm II test. 128 samples were classified as tetracyclines 110 (95.5%), ${\beta}$-lactam 2 (1.6%), sulfonamide 22 (17.2%), quinolone 1 (0.8%). The highest residual concentration of oxytetracycline, tetracycline, chlor-tetracycline, penicillin, sulfamethazine, sulfadimethoxine, sulfaquinoxaline, sulfamerazine, sulfamonomethoxine and enrofloxacin were 7.57, 0.27, 0.40, 0.24, 14.24, 4.33, 8.59, 0.12, 0.09 and 1.98 ppm, respectively and 49 samples were exceeded legal admitted levels.

• 한국동물위생학회지
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• 제27권3호
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• pp.265-272
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• 2004

It was carried out to compare the residual materials by EEC 4-plate, Charm II and HPLC method in the muscles of cattle and pigs from slaughter-houses in Seoul from 2000 to 2003. Residual materials were detected from $1.10\%$(73/6,623) samples by EEC 4-plate method, and $10.93\%$(55/503) samples by Charm II method. The highest residual concentration(ppm) of oxytetracycline, tetracycline, chloramphenicol, chlortetracycline, sulfamethazine, sulfamerazine, sulfadimethoxine, penicillin and sulfamonomethoxine were 25.5, 3.46, 3.26, 1.5, 0.3, 0.2, 0.2, 0.14, and 0.07, respectively. Eighty nine samples were classified as 58($65.17\%$) only tetracyclines, 20($22.47\%$) only sulfonamides, 3($3.37\%$) only ${\beta}$-lactams, 2($2.25\%$) only chloramphenicol, 4($4.49\%$) tetracyclines and sulfonamides simultaneously, 1($1.12\%$) chloramphenicol and sulfonamides simultaneously, and 1($1.12\%$) chloramphenicol, sulfonamides and tetracyclines simultaneously.

• 한국동물위생학회지
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• 제20권2호
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• pp.225-233
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• 1997

In an effort to Improve the quality of meat and to monitor farms, residual tetracyclines in local beef and pork produced in the province of North Chung-cheong were determined by a EEC 4-plate, Charm II and HPLC, respectively. The results were summarized as follows ; 1. Of the 547 samples, 4 beef and 13 pork samples were judged to be positive by EEC 4 - plate method. 2. Detection rates of tetracyclines by type 1(++--) and type 2(+++ or ++$\pm$-) micro-bial growth Inhibition In EEC 4-plate method were 100% and 71%, respectively. 3. Of 17 positive samples, 6 were positive for tetracyclines, 4 were positive for tetracyclines and sulfonamides, 1 was positive for sulfonamides, and 2 were positive for others by Charm II test. 4. The best eluents were 0.01M methanolic oxalic acid, and the ideal temperature for stable concentration was $40^{\circ}C$ as optimal HPLC analytical conditions for the detection of tetracy-clines. 5. Of the 10 positive samples for tetracyclines by Charm II test, tetracyclines were confirmed in 2 beef and 6 pork samples, using HPLC, at levels ranging from 3.64~4.22 ppm and 0.2~1.20 ppm, respectively.