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A Study on the Production of Yeast Utilizing Ethanol as a Sole Carbon Source (Ethanol 이용 미생물에 의한 단세포 단백질 생산에 관한연구)

  • Lee, Ke-Ho;Ha, Jin-Hong
    • Applied Biological Chemistry
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    • v.16 no.1
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    • pp.1-11
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    • 1973
  • In order to obtain the basic informations on the production of single cell protein from ethanol, 145 yeast strains utilizing ethanol as a sole carbon source were isolated from 32 soil samples in Korea. A yeast strain showing the highest cell yield among the isolated strains was selected and identified. The optimum culture condition, utilization of other carbon sources and the cultural characteristics for the selected yeast, and the chemical analysis of the yeast cell composition, and utilization of ethanol by the selected yeast were investigated. All the culture was carried out in the shaking flasks. The results obtained were as follows: 1. The selected yeast strain was identified as Debaryomyces nicotianae-SNU 72. 2. The optimum composition of the medium for the selected yeast is : Ethanol 40 ml, Urea 0.5 g, Potassium phosphate (dibasic) 0.5 g, Ammoium phosphate (monobasic) 0.15 g, Magnesium sulfate 0.05 g, Calcium chloride 0.01g, Yeast extract 0.005 g, Tap water 1000 ml. 3. The optimum pH was 5.0-5.5, the optimum temperature $30-33^{\circ}C$ and the aerobic state was unimportant. 4. Utilization of methanol, n-propanol, iso-propanol, n-butanol, iso-butanol, tert-amyl alcohol and acetic acid by the selected yeast was very weak. So substitution of the subtrate was thought to be impossible. 5. Studies on the propagation of the yeast cells showed that the lag phase of the yeast cells lasted 16 hours, and the logarithmic growth phase extended 16 to 28 hours. The specific growth rate was about $0.19\;hr^{-1}$ and the doubling time was 3.6 hours during the logarithmic growth phase. 6. As the result of the chemical analysis of the dry yeast cells, the content rate of the crude protein was 55.19 %, the content of others was similar to the average content of the yeast component. 7. After 34 hours cultivation, under the optimum culture condition investigated, the dry cell yield against the amount of the added ethanol was 53.4 % (W/V%), the dry cell yield against the amount of the utilized ethanol was 73.6 % (W/V%), the evaporation rate of ethanol was about 19.1 %.

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Lipid Composition of Improved Rape Seed on the Content of Erucic acid (에루신산에 대(對)한 개량(改良) 유채종자(油菜種子)의 지질구성(脂質構成) 성분(成分))

  • Lee, Sung-Ho;Kim, Chung-Ki;Kim, Hong-Su
    • Applied Biological Chemistry
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    • v.28 no.4
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    • pp.245-251
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    • 1985
  • In order to analyze the lipid composition ana chemical characteristics of rapeseed oil in relation to content of erucic acid, three recommendable cultivars(MOK-PO 70, MOK-PO DANGYO 12 and 14) seeds were used. The composition of lipid was confirmed by column chromatography and TLC, and the composition of fatty acid in total and neutral lipid was determined by GLC, and the results were as follows. 1. The crude fat was 44.0% to 45.3% in all cultivars. 2. The iodine value, saponification value, acid value and nonsaponifiable content were 100 to 101, 176 to 184, 0.7 to 0.9 and 1.0 to 1.2, respectively, with no remarkable difference among three cultivars. 3. The content of neutral lipid was 95.3% to 96.3% of total lipid, and others were compound lipids. 4. The content to triglyceride was 92. 1% to 92.5% of total lipid. Diglyceride and sterol ester were contained 1.1% and 1.2%, respectively. There was a small quantity of free fatty acid and free sterol and monoglyceride was little. 5. The erucic acid was the highest (26.4%), in MOK-PO DANGYO 12 among the composition of fatty acid, while those of MOK-PO 70 and MOK-PO DANGYO 14 were so small as 1.0% and 3.9%. The content of erucic acid was decreased with the increase of oleic acid, the content of oleic acid in MOK-PO DANGYO 12 was 30%, 63% in MOK-PO 70 and, 60% in MOK-PO DANGYO 14. The content of linoleic acid and linolenic acid showed no remarkable difference but that of eicosenoic acid was 11.7% in MOK-PO DANGYO 12 as the highest. The ratio of the fatty acid content in eluted neutral lipid and the total oil showed no remarkable differences.

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Optimization for the Process of Ethanol of Persimmon Leaf(Diospyros kaki L. folium) using Response Surface Methodology (반응표면분석법을 이용한 감잎(Diospyros kaki L. folium) 에탄올 추출물의 최적화)

  • Bae, Du-Kyung;Choi, Hee-Jin;Son, Jun-Ho;Park, Mu-Hee;Bae, Jong-Ho;An, Bong-Jeon;Bae, Man-Jong;Choi, Cheong
    • Applied Biological Chemistry
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    • v.43 no.3
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    • pp.218-224
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    • 2000
  • The efforts were made to optimite ethanol extraction from persimmon leaf with the time of extraction$(1.5{\sim}2.5\;hrs)$, the temperature of extraction$(70{\sim}90^{\circ}C)$, and the concentration of ethanol$(0{\sim}40%)$ as three primary variables together with several functional characteristics of persimmon leaf as reaction variables. The conditions of extraction was best fitted by using response surface methodology through the center synthesis plan, and the optimal conditions of extraction were established. The contents of soluble solid and soluble tannin went up as the concentration of ethanol went up and the temperature of extraction went down, and the turbidity went down as the concentration of ethanol went down. Electron donation ability was hardly affected by the extraction temperature and had the tendency to go up as the concentration of ethanol went up. The inhibitory activity of xanthine oxidase(XOase) had the tendency to go up as both the concentration of ethanol and the temperature of extraction went up. The inhibitory activity of angiotensin converting enzyme(ACE), the significance of which still was not recognized, showed the maximum when the concentration of ethanol was 27%. In result, the optimal conditions of extraction was the extraction time of two hours, the extraction temperature of $75{\sim}81^{\circ}C$, and the ethanol concentration of $33{\sim}35%$.

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Enhanced Anti-oxidant Activity Effects of Smilax china L. Rhizome Water Extracts Added with Its Fermented Leaf Water Extracts (발효 청미래덩굴잎 추출물의 혼합에 의한 토복령의 항산화활성 증진효과)

  • Lee, Sang-Il;Lee, Ye-Kyung;Kim, Soon-Dong;Shim, Soon-Mi;Yang, Seung Hwan;Cheng, Jinhua;Suh, Joo-Won
    • Journal of Applied Biological Chemistry
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    • v.57 no.2
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    • pp.145-152
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    • 2014
  • To evaluate the improving effects of antioxidant activity, we observed antioxidant capacities such as electron donating ability (EDA), Ferric reducing antioxidant power (FRAP), inhibitory activity of xanthine oxidase (XO) and aldehyde oxidase (AO), and sensory characteristics on mixture of Smilax china L. root water extract added with water extract of fermented S. china L. leaf by Aspergillus oryzae (FSCL). Those contents of mixture with higher ratio of FSCL were proportionally high. And OD475 of mixture with higher ratio of FSCL was almost proportionally high ($R^2=0.9850$). Antioxidant capacities of EDA and FRAP of the mixture was higher than that of non-mixture. In addition, XO inhibitory activity ($IC_{50}$) of A (1.19) was 59.80% higher than that of F (2.96), and the activity of mixture by the higher ratio of FSCL was proportionally low ($R^2=0.9490$). Taste acceptability of A was slightly higher than that of F, whereas that of C was highest. And color acceptability of 40-80% mixture was higher than those of A, F, and B. Overall acceptability of C and D was highest than those of others. Moreover, hot water extract of S. china L. leaf fermented with A. oryzae was maroon color, which looks like Puerh tea style, and mixture of S. china L. root extract added with hot water extract of S. china L. leaf was high acceptability of beverage. These results suggest that mixture of extract of S. china L. root and hot water extract of S. china L. leaf fermented with A. oryzae could improve antioxidant activities.

Characteristics of Tillering as Affected by Temperature Variation in Dasanbyeo, a Indica/Japonica High Yielding Rice Cultivar (온도 수준에 따른 다수성 벼 품종 ″다산벼″의 분얼 특성)

  • 김덕수;양원하;신진철;류점호
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.3 no.1
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    • pp.22-29
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    • 2001
  • In Korean high yielding varieties developed by crosses between indica and japonica rice, the most limiting factor for yield may be attributed to the less number of the tillers in the unit area. The goals of this study is to find out the effect of the temperature factors as well as cultural practices on the development and increase of tillers of Dasanbyeo, the high yielding indica crossed japonica cultivar. The effect of temperature was examined under controlled phytotron condition with 6 levels of temperature, 15, 17, 19, 22, 24 and 26$^{\circ}C$, respectively, For the experiment, the leading japonica variety in Korea, Hwaseongbyeo, was used for the check cultivar for the comparison with Dasanbyeo. The high temperature also accelerated the initiation and termination of tiller development. The cultivar difference in the speed of tiller development was observed, for example, more rapid development of tiller in Dasanbyeo than in Hwaseongbyeo was observed at the high temperature range of 24-26$^{\circ}C$, while the vice versa phenomena was observed at lower temperature range of 17-22$^{\circ}C$. The first secondary tiller of Dasanbyeo was observed on the 16, 17, 23, 27 and 38 days after transplanting (DAT) at 26$^{\circ}C$, 24$^{\circ}C$, 22$^{\circ}C$, 19$^{\circ}C$, and 17$^{\circ}C$, respectively. Those of Hwaseongbyeo was 19-22, and 26 DAT at 19-26$^{\circ}C$ and 17$^{\circ}C$, respectively. The last effective tiller of Dasanbyeo was observed on 27-33 DAT for the primary tiller, 20-41 DAT for the secondary tiller. Those of Hwaseongbyeo were 23-40 DAT for primary tiller, and 24-40 DAT for the secondary tiller, and 24-40 DAT for the secondary tiller.

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Characteristics of Tillering as Affected by Light intensity in Dasanbyeo, an Indica/Japonica High Yielding Rice Cultivar (광도 변화에 따른 다산벼의 분얼경 발생 특성)

  • 김덕수;양원하;신진철;김제규;류점호
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.4 no.3
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    • pp.151-158
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    • 2002
  • In Korean high yielding varieties developed by crosses between indica and japonica rice, the most limiting factor for yield increase may be attributed to the less number of tillers per unit area. The goal of this study is to find out the effect of the environmental factors as well as cultivation practice on the development and increase of tillers of Dasanbyeo, the high yielding indica / japonica hybrid cultivar. The effect of temperature was examined with 3 different light intensity, 220,600, and 1220 $\mu$㏖/s/$m^2$, respectively. For all the experiments, the leading japonica variety in Korea, Hwaseongbyeo, was used fur the check cultivar for the comparison with Dasanbyeo. The increase of the tillers was more prominent in Dasanbyeo than in Hwaseongbyeo at 220 $\mu$㏖/s/$m^2$ of light intensity, while the similar increase of tiller no. was found at 600 $\mu$㏖/s/$m^2$ of light intensity treatment. However, Hwaseongbyeo showed more rapid increase of tiller at 1220 $\mu$㏖/s/$m^2$ of light intensity. The mean number of the primary tiller ranged 5 to 7 in Dasanbyeo, and 2 to 7 in Hwaseongbyeo, showing greater variation in the latter case. However, the secondary tiller number ranged from 2 to 13 for the former, and 2 to 12 for the latter. The earliest initiation of tiller node of Dasanbyeo and Hwaseongbyeo was observed on 6 and 4 days after transplanting(DAT), respectively, at 600 $\mu$㏖/s/$m^2$ of light intensity, while 10, and 7 DAT, respectively, at 1,220 $\mu$㏖/s/$m^2$. No cultivar difference was observed at 600 $\mu$㏖/s/$m^2$ with the 18 DAT. The ratio of effective tiller was lower in Dasanbyeo, ranging from 47 to 55% than in Hwaseongbyeo, ranging from 72 to 100%.

Exploring Ways to Improve the Predictability of Flowering Time and Potential Yield of Soybean in the Crop Model Simulation (작물모형의 생물계절 및 잠재수량 예측력 개선 방법 탐색: I. 유전 모수 정보 향상으로 콩의 개화시기 및 잠재수량 예측력 향상이 가능한가?)

  • Chung, Uran;Shin, Pyeong;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.19 no.4
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    • pp.203-214
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    • 2017
  • There are two references of genetic information in Korean soybean cultivar. This study suggested that the new seven genetic information to supplement the uncertainty on prediction of potential yield of two references in soybean, and assessed the availability of two references and seven genetic information for future research. We carried out evaluate the prediction on flowering time and potential yield of the two references of genetic parameters and the new seven genetic parameters (New1~New7); the new seven genetic parameters were calibrated in Jinju, Suwon, Chuncheon during 2003-2006. As a result, in the individual and regional combination genetic parameters, the statistical indicators of the genetic parameters of the each site or the genetic parameters of the participating stations showed improved results, but did not significant. In Daegu, Miryang, and Jeonju, the predictability on flowering time of genetic parameters of New7 was not improved than that of two references. However, the genetic parameters of New7 showed improvement of predictability on potential yield. No predictability on flowering time of genetic parameters of two references as having the coefficient of determination ($R^2$) on flowering time respectively, at 0.00 and 0.01, but the predictability of genetic parameter of New7 was improved as $R^2$ on flowering time of New7 was 0.31 in Miryang. On the other hand, $R^2$ on potential yield of genetic parameters of two references were respectively 0.66 and 0.41, but no predictability on potential yield of genetic parameter of New7 as $R^2$ of New7 showed 0.00 in Jeonju. However, it is expected that the regional combination genetic parameters with the good evaluation can be utilized to predict the flowering timing and potential yields of other regions. Although it is necessary to analyze further whether or not the input data is uncertain.

Effects of Drought Stress and Nitrogen Fertilization on Growth and Physiological Characteristics of Pinus densiflora Seedlings Under Elevated Temperature and CO2 Concentration (대기 중 온도 및 CO2 농도 조절에 따른 건조 스트레스와 질소 시비가 소나무의 생장 및 생리적 특성에 미치는 영향)

  • Song, Wookyung;Lee, Bora;Cho, Nanghyun;Jung, Sungcheol;Kim, Eun-Sook;Lim, Jong-Hwan
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.2
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    • pp.57-67
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    • 2020
  • Pinus densiflora is the most widely distributed tree species in South Korea. Its ecological and socio-cultural attributes makes it one of the most important tree species in S. Korea. In recent times however, the distribution of P. densiflora has been affected by dieback. This phenomenon has largely been attributed to climate change. This study was conducted to investigate the responses of growth and physiology of P. densiflora to drought and nitrogen fertiliz ation according to the RCP 8.5 scenario. A Temperature Gradient Chamber (TGC) and CO2. Temperature Gradient Chamber (CTGC) were used to simulate climate change conditions. The treatments were established with temperature (control versus +3 and +5℃; aCeT) and CO2 (control: aCaT versus x1.6 and x2.2; eCeT), watering(control versus drought), fertilization(control versus fertilized). Net photosynthesis (Pn), stomatal conductance (gs), biomass and relative soil volumetric water content (VWC) were measured to examine physiological responses and growth. Relative soil VWC in aCeT significantly decreased after the onset of drought. Pn and gs in both aCeT and eCeT with fertiliz ation were high before drought but decreased rapidly after 7 days under drought because nitrogen fertilization effect did not last long. The fastest mortality was 46 days in aCeT and the longest survival was 56 days in eCeT after the onset of drought. Total and partial biomass (leaf, stem and root) in both aCeT and eCeT with fertiliz ation were significantly high, but significantly low in aCeT. The results of the study are helpful in addressing P. densiflora vulnerability to climate change by highlighting physiological responses related to carbon allocation under differing simulated environmental stressors.

Detection of Phantom Transaction using Data Mining: The Case of Agricultural Product Wholesale Market (데이터마이닝을 이용한 허위거래 예측 모형: 농산물 도매시장 사례)

  • Lee, Seon Ah;Chang, Namsik
    • Journal of Intelligence and Information Systems
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    • v.21 no.1
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    • pp.161-177
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    • 2015
  • With the rapid evolution of technology, the size, number, and the type of databases has increased concomitantly, so data mining approaches face many challenging applications from databases. One such application is discovery of fraud patterns from agricultural product wholesale transaction instances. The agricultural product wholesale market in Korea is huge, and vast numbers of transactions have been made every day. The demand for agricultural products continues to grow, and the use of electronic auction systems raises the efficiency of operations of wholesale market. Certainly, the number of unusual transactions is also assumed to be increased in proportion to the trading amount, where an unusual transaction is often the first sign of fraud. However, it is very difficult to identify and detect these transactions and the corresponding fraud occurred in agricultural product wholesale market because the types of fraud are more intelligent than ever before. The fraud can be detected by verifying the overall transaction records manually, but it requires significant amount of human resources, and ultimately is not a practical approach. Frauds also can be revealed by victim's report or complaint. But there are usually no victims in the agricultural product wholesale frauds because they are committed by collusion of an auction company and an intermediary wholesaler. Nevertheless, it is required to monitor transaction records continuously and to make an effort to prevent any fraud, because the fraud not only disturbs the fair trade order of the market but also reduces the credibility of the market rapidly. Applying data mining to such an environment is very useful since it can discover unknown fraud patterns or features from a large volume of transaction data properly. The objective of this research is to empirically investigate the factors necessary to detect fraud transactions in an agricultural product wholesale market by developing a data mining based fraud detection model. One of major frauds is the phantom transaction, which is a colluding transaction by the seller(auction company or forwarder) and buyer(intermediary wholesaler) to commit the fraud transaction. They pretend to fulfill the transaction by recording false data in the online transaction processing system without actually selling products, and the seller receives money from the buyer. This leads to the overstatement of sales performance and illegal money transfers, which reduces the credibility of market. This paper reviews the environment of wholesale market such as types of transactions, roles of participants of the market, and various types and characteristics of frauds, and introduces the whole process of developing the phantom transaction detection model. The process consists of the following 4 modules: (1) Data cleaning and standardization (2) Statistical data analysis such as distribution and correlation analysis, (3) Construction of classification model using decision-tree induction approach, (4) Verification of the model in terms of hit ratio. We collected real data from 6 associations of agricultural producers in metropolitan markets. Final model with a decision-tree induction approach revealed that monthly average trading price of item offered by forwarders is a key variable in detecting the phantom transaction. The verification procedure also confirmed the suitability of the results. However, even though the performance of the results of this research is satisfactory, sensitive issues are still remained for improving classification accuracy and conciseness of rules. One such issue is the robustness of data mining model. Data mining is very much data-oriented, so data mining models tend to be very sensitive to changes of data or situations. Thus, it is evident that this non-robustness of data mining model requires continuous remodeling as data or situation changes. We hope that this paper suggest valuable guideline to organizations and companies that consider introducing or constructing a fraud detection model in the future.

Personalized Recommendation System for IPTV using Ontology and K-medoids (IPTV환경에서 온톨로지와 k-medoids기법을 이용한 개인화 시스템)

  • Yun, Byeong-Dae;Kim, Jong-Woo;Cho, Yong-Seok;Kang, Sang-Gil
    • Journal of Intelligence and Information Systems
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    • v.16 no.3
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    • pp.147-161
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    • 2010
  • As broadcasting and communication are converged recently, communication is jointed to TV. TV viewing has brought about many changes. The IPTV (Internet Protocol Television) provides information service, movie contents, broadcast, etc. through internet with live programs + VOD (Video on demand) jointed. Using communication network, it becomes an issue of new business. In addition, new technical issues have been created by imaging technology for the service, networking technology without video cuts, security technologies to protect copyright, etc. Through this IPTV network, users can watch their desired programs when they want. However, IPTV has difficulties in search approach, menu approach, or finding programs. Menu approach spends a lot of time in approaching programs desired. Search approach can't be found when title, genre, name of actors, etc. are not known. In addition, inserting letters through remote control have problems. However, the bigger problem is that many times users are not usually ware of the services they use. Thus, to resolve difficulties when selecting VOD service in IPTV, a personalized service is recommended, which enhance users' satisfaction and use your time, efficiently. This paper provides appropriate programs which are fit to individuals not to save time in order to solve IPTV's shortcomings through filtering and recommendation-related system. The proposed recommendation system collects TV program information, the user's preferred program genres and detailed genre, channel, watching program, and information on viewing time based on individual records of watching IPTV. To look for these kinds of similarities, similarities can be compared by using ontology for TV programs. The reason to use these is because the distance of program can be measured by the similarity comparison. TV program ontology we are using is one extracted from TV-Anytime metadata which represents semantic nature. Also, ontology expresses the contents and features in figures. Through world net, vocabulary similarity is determined. All the words described on the programs are expanded into upper and lower classes for word similarity decision. The average of described key words was measured. The criterion of distance calculated ties similar programs through K-medoids dividing method. K-medoids dividing method is a dividing way to divide classified groups into ones with similar characteristics. This K-medoids method sets K-unit representative objects. Here, distance from representative object sets temporary distance and colonize it. Through algorithm, when the initial n-unit objects are tried to be divided into K-units. The optimal object must be found through repeated trials after selecting representative object temporarily. Through this course, similar programs must be colonized. Selecting programs through group analysis, weight should be given to the recommendation. The way to provide weight with recommendation is as the follows. When each group recommends programs, similar programs near representative objects will be recommended to users. The formula to calculate the distance is same as measure similar distance. It will be a basic figure which determines the rankings of recommended programs. Weight is used to calculate the number of watching lists. As the more programs are, the higher weight will be loaded. This is defined as cluster weight. Through this, sub-TV programs which are representative of the groups must be selected. The final TV programs ranks must be determined. However, the group-representative TV programs include errors. Therefore, weights must be added to TV program viewing preference. They must determine the finalranks.Based on this, our customers prefer proposed to recommend contents. So, based on the proposed method this paper suggested, experiment was carried out in controlled environment. Through experiment, the superiority of the proposed method is shown, compared to existing ways.