• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.16 no.4
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• pp.324-333
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• 2006

This study was designed to investigate the effect of bone demineralization and tibia lead on blood lead in retired lead workers. Two hundred thirty five(126 females and 109 males) retired lead workers who worked in 4 different lead factories and 101 non-occupationally lead exposed subjects(51 females and 51 males) were recruited from March 2004 to October 2004. Bone mineral density(BMD) was measured at left calcaneous bone area by broadband ultrasound attenuation(BUA) method with QUS-2(Metra Biosystems Inc, USA). The BUA value transformed into T-score by WHO standard conversion criteria. Tibia bone lead was measured for skeletal bone lead with K-xray fluorescence(K-XRF) and blood lead was analyzed with flameless atomic spectrophotometer. Hemoglobin, hematocrit, serum calcium and iron were also analyzed. In addition, information for smoking and drinking status and basic personal data such as age, gender and lead exposure were also collected using questionnaire inquiry. Blood lead was correlated with tibia lead (r=0.664) and these two variables were negatively correlated with BMD in bivariate analysis. BMD showed significant main effect on the change of blood lead independent to tibia lead without any effect modification of age or gender; the one T-score unit decrease of mineral bone density made $0.43{\mu}g/dl$ increase of blood lead. On the other hand, tibia lead showed effect modification with gender on blood lead; the slope of tibia lead on blood lead in male was steeper than in female and crossed at around zero of tibia lead. In the multiple regression analysis of blood lead and tibia lead on BMD after adjustment of related covariates, only blood lead showed statistically significant effect on BMD. This study confirmed that BMD and blood lead were significantly associated. To verify the causal association of BMD on blood lead and vice versa, further longitudinal studies are needed.

• Korean Journal of Veterinary Service
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• v.31 no.2
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• pp.175-186
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• 2008

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

• Journal of fish pathology
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• v.8 no.2
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• pp.149-156
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• 1995

Physiological activities of E. compressa were examined after oral and intraperitoneal(i.p.) adminstration in young(8g) Israeli and colored carp. Hematological parameters were evaluated to test physiological respose. Anti-bacterial activity was examined by counting the number of bacterial cells in the kidney, and also by measuring the change of agglutinin titers following A. sobriae infection. There was a tendency of increase in E. compressa-fed groups in total protein, albumin and glucose levels. The most marked increase was noted in the group fed with 5% E. compressa. GPT and GOT levels were reduced with the increase of E. compressa concentration. Feeding of E. compressa did not alter haematocrit(Ht) and hemoglobin (Hb) values. The number of A. sobriae was reduced in all groups intraperitoneally treated with variable concentration of E. compressa-extract. The lowest bacterial cells were found at the group intraperitoneally treated with $30{\mu}g$ of E. compressa-extract per g of colored carp($30{\mu}g/g$), indicating that the anti-bacterial activity is maximized at this concentration. The agglutinin titers were elevated in E. compressa extract-treated groups($30{\mu}g/g$) with the maximum value of $6.0{\pm}1.1$. These results indicate that E. compressa adminstration activated physiological response, and triggered a cascade for anti-bacterial reaction.

• Korean Journal of Clinical Laboratory Science
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• v.38 no.1
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• pp.16-21
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• 2006

Parallel testing means ordering a number of tests at the same time so abnormalities in any of the tests can be found quickly and used in making the diagnosis. This is a good medical strategy to eliminate diseases and it is relatively inexpensive if all the tests are potential sources of information and performed on the same analyzer. In regression, the equation for the straight line is recast as y = bx + a. This change in terminology leads to confusion. Here a is the y-intercept or constant and b is the coefficient or slope of the line. A few more words of caution about regression - as in all of statistics there are certain assumptions: the x value is a true measure, both X and Y distributions are normal, and homoscedasticity, i.e., the variance of y is the same for each value of x. In this study the linearity classification made by different scientists were always in agreement. Typical examples of curves that were considered linear are presented in Fig. 1-5. Because these automated procedures values were usually within five percent of each other the curvature could be easily detected. The plot of the WBC, RBC, hemoglobin, hematocrit and platelet concentrations from approximately 74.4 to $0{\times}10^3/{\mu}L$ and $80.4-0{\times}10^3/{\mu}L$, $5.6-0{\times}10^6/{\mu}L$ and $6.1-0{\times}1106/{\mu}L$, 18.3-0 g/dL and 19.0-0 g/dL, 54.1-0% and 56.8-0% and 642.0 to $0.03{\times}10^3{\mu}L$ and $754.0-0{\times}10^3/{\mu}L$ on the ADVIA 2120 C Versus and A and B typical of an acceptable linear study as shown in Fig. 1-5. The grand mean of R2, intercept and slope is 0.99898, 0.99459 and 1.54626.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.3
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• pp.357-365
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• 2002

A survey was under taken of six district of Northern India viz. Bareilly, Pilibhit, Udham Singh Nagar, Nainital, Almora and Rampur. The age, breed, sex and physiological status recorded. A total number of 854 cattle examined out of which lactating (274 cases), non lactating (302 cases) heifers (128 cases), calves (82 cases) and adult male (68 cases) were examined. An incidence of 4.92 percent (42) of microfilarisis was recorded. The highest prevalence was observed in Rudrapur District of Udham Singh Nagar (33.33%, 4/12), followed by Lalkaun in Nanital District (21.74%, 10/46), Rampur (12.50%, 2/16), Bareilly (8.16%, 8/98) and Pilibhit (1.22%, 1/82). No infection was observed in Almora region. Amongst 854 cattle of different group incidence was highest in adult male (12.20%, 10/82), followed by non lactating (3.82%, 12/314) and lactating (2.70%, 2/74), (7.64%, 12/157) was found in Heifers. For haemeto-biochemical, serum minerals estimations and therapeutic study 32 animals suffering from filariasis and 18 healthy animals were taken. 16 animals were treated with ivermectin $@200{\mu}g/kg $ body weight. Effect of this disease on production has also been estimated for which body weight and milk production was observed. The main clinical manifestations observed were anaemia, loss of appetite, debility, oedematous swelling especially in the abdominal region, increased heart rate, and respiration rate. Haematological changes indicated decrease in hemoglobin, total erythrocyte count, packed cell volume, erthrocyte fragility and neutrophil, whereas there was significant increase in erythrocytes sedimentation rate (ESR), total leukocyte count (TLC), lymphocyte and eosinophils. Biochemical changes showed significant reduction in the values of serum albumin, A : G ratio, where as there was significant increase in blood glucose, blood urea nitrogen (BUN), globulin, total lipid, total cholesterol, phospholipids, serum bilirubin. Serum mineral profile also altered markedly, which indicate a significant decrease in Ca, Cu, Fe, Zn, and Mn with increase value of Na and Cl. There was no significant change in P and K values. Enzyme pattern in micro filaria infected animal indicated increased level of AST, ALT, alkaline phosphatase, ornithine carbamyl transferase, sorbitol dehydrogenase, glutamate dehydrogenase, isocitric dehydrogenase and lactate dehydrogenase. In blood gas values and acid/base balance, there was an increase in $PVCo_2$ and $PVo_2$. It has been observed that microfilaria infected cattle showed decrease in body weight and milk production. Animal treated with ivermectin showed the return of these above values toward normalcy.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.2
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• pp.105-111
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• 2020

Smoking is associated with increased inflammation and is a risk factor for a range of diseases. Conversely, yogurt has beneficial effects on health. This study examined the effects of yogurt consumption on the hematological parameters and fibrinogen levels in smokers. The participants were categorized into four groups. Non-smokers were assigned to the control and yogurt groups while smokers were assigned to the smoking and combined (yogurt plus smoking) groups. The participants consumed yogurt, and either smoked or did not smoke for three weeks. The changes in hematological parameters and fibrinogen levels were examined. In the combined group, the proportion of neutrophils and fibrinogen levels increased significantly, whereas the lymphocyte proportion decreased. The eosinophil proportion increased significantly in the Smoking group, but no change was observed in the combined group. In addition, the mean corpuscular hemoglobin concentration increased in the smoking and combined groups, indicating that smoking may affect the lifespan of red blood cells. In conclusion, this study showed that yogurt consumption might influence the immune function by modulating the levels of neutrophils, lymphocytes, and fibrinogen in smokers. Furthermore, the absence of any increase in the eosinophil proportion in the combined group, unlike in the smoking group, suggests that yogurt consumption may have an inhibitory effect on allergic reactions.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.10
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• pp.1435-1441
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• 2017

Objective: The objective of this study was to determine the effect of adding water to total mixed ration (TMR) on fresh water intake, nutrient digestibility, wool cortisol, and blood indices in Corriedale ewes under hot and humid conditions. Methods: Nine non-pregnant Corriedale ewes (ave. body weight = $41{\pm}3.5kg$) were individually fed diets based on maintenance requirements in metabolic crates. Ewes were assigned to three treatment groups according to a triplicate $3{\times}3$ Latin Square design for 3 periods of 21 days duration each (9 ewes per treatment, 27 replications). Treatments were TMR (crude protein [CP] = 16.1, total digestible nutrients = 69.1%) moisture levels for 40%, 50%, and 60%. Results: No differences were found in body weight gain among all treatment groups (p>0.05). Nitrogen balance including digestible N, retained N, and urinary and fecal N showed no change among the treatment groups (p>0.05). Fresh water intake was the lower in 50% TMR moisture group than in the other groups (p<0.05). Other than ether extract which was higher in 60% TMR moisture group (p<0.05) the differences among nutrient digestibilities including CP, organic matter, dry matter, neutral detergent fiber, acid detergent fiber, and non-fiber carbohydrate were not significant (p>0.05). No significant difference was observed for serum protein, blood urea nitrogen, glucose, and triglyceride among the treatment groups (p>0.05). Wool and blood cortisol were not different among the treatment groups (p>0.05). Blood hematology including red blood cell, white blood cells, hemoglobin, hematocrit, basophils, and eosinophils were not different among the treatment groups (p>0.05). Conclusion: It is concluded that TMR moisture at 40%, 50%, and 60% had no effects on N balance parameters, and nutrient digestibilities except for the ether extract under hot and humid conditions. Additionally there were no effects on stress conditions include wool cortisol, as well as blood cortisol levels of ewes.

• Journal of the Korean Institute of Intelligent Systems
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• v.15 no.4
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• pp.473-478
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• 2005

The gastric cancer takes the great part of the cancer occurrence and the mortality from cancer in Korea, and the early detection of gastric cancer is very important in the treatment and convalescence. This paper. for the early detection of gastric cancer, Proposes the analysis system of endoscopic image of the stomach that detects the abnormal region by using the change of color in the image and provides the surface tissue information to the detector. While the advanced inflammation and the cancer may be easily detected, the early inflammation and the cancer have a difficulty in detection and require the more attention lot detection. This paper, at first, converts the endoscopic image to the Image of IHb(Index of Hemoglobin) model and removes noises incurred by illumination, and next, automatically detects the regions suspected as cancer and provides the related information to the detector, or provides the surface tissue information for the regions appointed by the detector. This paper does not intend to provide the final diagnosis of the detected abnormal regions as gastric cancer, but provides the supplementary mean that reduces the load and mistaken diagnosis of the detector by automatically detecting the abnormal regions being not easily detected by human eyes and providing the additional information for the diagnosis. The experiments using practical endoscopic images for performance evaluation showed that the proposed system is effective in the analysis of endoscopic image of the stomach.

• Journal of Ginseng Research
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• v.30 no.4
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• pp.199-205
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• 2006

It was investigated that how the periodic exposure to heat environment, and the treatment of korean white and red ginseng extracts had effects on the weight, diet uptake, blood components, organ weight, and the lipid peroxidation of liver in male S.D. rats. In the result of experiments using rats, chronic heat environment for 7 days at $38^{\circ}C$, 5 hrs per day, induced significant decrease of an average increase rate of body weight, but diet uptake was not affected clearly. In heat environment, the number of red blood cells and hemoglobin were not changed, but the number of white blood cells was significantly increased. The liver weight against body weight was decreased in rats. Also, MDA contents, related to lipid peroxidation, were remarkably increased in rat liver by heat environment. These physiological changes were attenuated by treatments of white and red ginseng extracts before and after exposure to heat environment, particularly in growth rate and lipid peroxidation of liver in rats. Also, red ginseng extracts had a better effect, though it was not that significant, than the white ginseng on the inhibition of lipid peroxidation and the weight change of liver. Although the investigation on the effective components and the study on the activity changes of associated materials are needed to perform, these present results imply that Korean ginseng may contribute to protection of body homeostasis against drastic climate changes.

• Journal of fish pathology
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• v.20 no.3
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• pp.269-279
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• 2007

This study was conducted to investigate the change of antioxidant enzyme activity (catalase and superoxide dismutase) and variation of blood physiology in olive flounder (Paralyticus olivaceus) by hydrogen peroxide (H2O2) treatment. Blood parameters were measured 1, 3 and 5 hours after H2O2 treatment with 0 (control), 100, 300 and 500 ppm for 1 hr. The value of hematocrit was decreased significantly dependently on treatment concentrate and elapsed time in the treatment of H2O2. Hemoglobin concentration in the test groups were lower than that of the control group. Red blood cell value in the test groups were significantly lower compared to that of the control group, but recovered to the level of the control group after 5 hr. Protein concentration was significantly lower compared to that of the control group at 0 and 1 hr, but recovered after 3 hr in 500 ppm treatment group. The superoxide dismutase (SOD) and catalase (CAT) enzyme activities were observed to be increased. Heat-shock protein 70 (HSP70) was significantly increased compared to that of control group in all of the test groups. HSP70 mRNA groups was highly expressed in 500 ppm treatment.