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Effects of the different hydrogen peroxide ($H_{2}O_{2}$) treatment level on physiological and biochemical responses of olive flounder (Paralichthys olivaceus)  

Choe, Mi-Kyung (Genetics & Breeding Research Center, National Fisheries Research & Development Institute)
Yeo, In-Kyu (Faculty of Applied Marine Science, Cheju National University)
Publication Information
Journal of fish pathology / v.20, no.3, 2007 , pp. 269-279 More about this Journal
Abstract
This study was conducted to investigate the change of antioxidant enzyme activity (catalase and superoxide dismutase) and variation of blood physiology in olive flounder (Paralyticus olivaceus) by hydrogen peroxide (H2O2) treatment. Blood parameters were measured 1, 3 and 5 hours after H2O2 treatment with 0 (control), 100, 300 and 500 ppm for 1 hr. The value of hematocrit was decreased significantly dependently on treatment concentrate and elapsed time in the treatment of H2O2. Hemoglobin concentration in the test groups were lower than that of the control group. Red blood cell value in the test groups were significantly lower compared to that of the control group, but recovered to the level of the control group after 5 hr. Protein concentration was significantly lower compared to that of the control group at 0 and 1 hr, but recovered after 3 hr in 500 ppm treatment group. The superoxide dismutase (SOD) and catalase (CAT) enzyme activities were observed to be increased. Heat-shock protein 70 (HSP70) was significantly increased compared to that of control group in all of the test groups. HSP70 mRNA groups was highly expressed in 500 ppm treatment.
Keywords
Olive flounder (Paralichthys olivaceus); Hydrogen peroxide($H_{2}O_{2}$); Superoxide dismutase(SOD); Catalase(CAT); Heat-shock protein(HSP);
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