• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.3
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• pp.354-361
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• 2015

Mitochondrial heat shock protein 75 (mtHSP75) is a member of the HSP90 family and plays essential roles in refolding proteins of the mitochondrial matrix. Mitochondria provide energy in the form of ATP and generate reactive oxygen species (ROS). Heat shock proteins (HSPs) are activated in response to stress, and protect cells. In this study, we characterized the mtHSP75 of the big-belly seahorse Hippocampus abdominalis. The protein (BsmtHSP75) is encoded by an open reading frame (ORF) of 2,157 nucleotides, has 719 amino acids (aa), and is of molecular mass 82 kDa. BsmtHSP75 has two functional domains, a histidine kinase-like ATPase (HATPase_c) domain (123-276 aa) and an HSP90 family domain (302-718 aa). BsmtHSP75 was expressed in all tested tissues of healthy seahorses. The ovary contained the highest transcription level, followed (in order) by the blood, brain, and muscle. Pouch tissue showed the lowest expression level. The expression of BsmtHSP75 was significantly (P<0.05) up-regulated on viral or bacterial challenge, suggesting that BsmtHSP75 plays a role in the immune defense against bacterial and viral pathogens.

• Proceeding of EDISON Challenge
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• 2016.03a
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• pp.246-255
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• 2016

When spring of the suspension is exerted by an external load, a car should be designed to prevent predictable damages and designed for a ride comfort. We used experiments design to design VON-MISES STRESS and K, a constant, of spring of suspension which is installed in a car as a goal level. We analyzed the result from Edison's Elastic - Plastic Analysis SW(CSD_EPLAST) by setting D, d, n as external diameter of coil, internal diameter of coil, the number of total coil respectively. The experiment design let the outcome be as Full-second order by using Box-Behnken which is one of response surface methods. Experimented and analyzed results based on the established experiments design, We found out design parameter which has desired VON-MISES STRESS and the constant K. Additionally, we predicted life time of when the external load was exerted by repeated load by using fatigue equation, and verification of plastic deformation has also been made. Additionally we interpreted a model, which is formed by optimized design parameter, with linear analysis and non-linear analysis, at the same time we also analyzed plastic deformation with the values from the both models. Finally, we predicted fatigue life of optimized model by using fatigue estimation theory and also evaluated a ride comfort with oscillation analysis.

• The Korean Journal of Physiology and Pharmacology
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• v.10 no.6
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• pp.309-315
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• 2006

The protective effects of baicalein, one of the flavonoids in Scutellaria baicalensis Georgi, were evaluated against 6-hydroxydopamine (6-OHDA)-induced neuronal damage in mice and cultured human neuroblastoma cells. Nigrostriatal damage was induced by stereotaxically injecting 6-OHDA into the right striatum. Baicalein was administered intraperitoneally 30 min before and 90 min after lesion induction. Animals received a further daily injection of baicalein for 3 consecutive days. Two weeks after 6-OHDA injection, contralateral rotational asymmetry was observed by apomorphine challenge in lesioned mice. Tyrosine hydroxylase (TH) immunohistochemistry revealed a significant loss of terminals in lesioned striatum and the reduction of the numbers of TH-positive cell in the ipsilateral substantia nigra (SN). In addition, the levels of dopamine (DA) and DA metabolites were reduced and lipid peroxidation was increased in lesioned striatum. However, baicalein treatment reduced apomorphine-induced rotational behavior in 6-OHDA-lesioned mice, and increased TH immunoreactivity in the striatum and SN, and DA levels in lesioned striatum. Lipid peroxidation induced by 6-OHDA was also inhibited by baicalein treatment. Furthermore, when SH-SY5Y human neuroblastoma cells were treated with baicalein, 6-OHDA-induced cytotoxicity and reactive oxygen species (ROS) production were significantly reduced. These results indicate that baicalein effectively protects 6-OHDA-induced neuronal damage through antioxidant action.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.734-741
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• 2019

Objective: The objective of the study was to determine the relationship between plasma and salivary cortisol concentrations in beef cattle that were subjected to handling prior to sampling. Methods: Twenty-one Nguni cows of three age categories; 5 to 7 yr (n = 7), 8 to 10 yr (n = 6), and 11 to 13 yr (n = 8) were handled for five consecutive weeks. In the pen, a human avoidance test was performed and cattle responses to restraint in the chute and crush were observed. In addition, rectal temperature readings were taken and, faecal samples were collected and analysed for glucocorticoid metabolites. Through the handling and restraint process, excretory and vocalisation behaviour, as a sign of stress were observed and recorded. Thereafter, six cows were randomly selected and subjected to an adrenocorticotropic hormone (ACTH) challenge. Blood and saliva samples were extracted to determine cortisol concentrations. Results: Repeated handling affected (p<0.05) faecal glucocorticoid metabolites, rectal temperatures, avoidance distance, crush scores as well as urination and defaecation behaviour. Acclimation to handling was variable based on each respective parameter. Saliva cortisol concentrations increased and decreased significantly (p<0.001). A peak value of $136.78{\pm}15.869nmol/L$ was observed 30min after administration of ACTH, from a baseline value of $8.75{\pm}15.869nmol/L$. Plasma cortisol concentrations did not differ (p>0.05) across the time of sampling. A low and insignificant correlation (r = 0.0131, p>0.05) between plasma and saliva cortisol was therefore observed. Conclusion: We conclude that if beef cows are subjected to handling prior to sampling, a weak relationship exists between plasma and salivary cortisol levels.

• Journal of The Korean Society For Urban Railway
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• v.6 no.4
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• pp.393-399
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• 2018

It is well recognized that residual stresses of the rails, generated from the manufacturing process including roller straightening and heat treatment, play an important role in determining fatigue and fracture properties of the rails. Thus, it has been a challenge to measure the residual stresses accurately. In this work, contour method was employed to evaluate the residual stresses existing in interior of the rails. The cross section perpendicular to the longitudinal direction of the rail was cut at a very slow rate using electric discharge machining (EDM), after which a laser-based flexural measuring instrument enabled us to precisely measure the flection of the cross section. The measured data were converted into the residual stresses using the commercial finite element package, ABAQUS, through a user-defined element (UEL) subroutine, and the residual stresses of the new rails (50N, KR60, UIC60) with three different specifications were compared.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.37 no.1
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• pp.41-47
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• 2024

This paper utilizes computational asymptotic analysis to compute the boundary layer solution for composite beams and validates the findings through a comparison with ANSYS results. The boundary layer solution, presented as a sum of the interior solution and pure boundary layer effects, necessitates a mathematically rigorous formalization for both interior and boundary layer aspects. Computational asymptotic analysis emerges as a robust technique for addressing such problems. However, the challenge lies in connecting the boundary layer and interior solutions. In this study, we systematically separate the principles of virtual work and the principles of Saint-Venant to tackle internal and boundary layer issues. The boundary layer solution is articulated by calculating the Papkovich-Fadle eigenfunctions, representing them as linear combinations of real and imaginary vectors. To address warping functions in the interior solutions, we employed a least squares method. The computed solutions exhibit excellent agreement with 2D finite element analysis results, both quantitatively and qualitatively. This validates the effectiveness and accuracy of the proposed approach in capturing the behavior of composite beams.

• International Journal of Highway Engineering
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• v.16 no.2
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• pp.61-71
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• 2014

PURPOSES : The first generation of wide base tires introduced in the early 1980s was found to cause a significant increase in pavement damage compared to dual-tire assemblies. However, wide base tires have evolved considerably, and a new generation of wide base tire is thought to be comparable to conventional dual tires for pavement damage. A challenge associated with using wide base tires is the accurate quantification of pavement damage induced by these tires. The objective of this study was to investigate the responses of flexible pavement to continuously moving vehicular loading under various tire configurations. METHODS : The comparison of the strain/stress responses of full-depth pavement caused by conventional dual tire assembly and new generation of wide-base tires was performed. The FE model incorporates linear viscoelasticity of asphalt material and continuous moving load using implicit dynamic analysis. RESULTS AND CONCLUSIONS : The result demonstrates that the new wide-base tires caused slightly more fatigue damage and less primary rutting damage in HMA layer than a dual-tire assembly, but caused more secondary rutting damage in subgrade than a dual tire assembly.

• Archives of Reconstructive Microsurgery
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• v.24 no.2
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• pp.68-74
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• 2015

Purpose: The reconstruction of femur and tibia defects following tumor resection remains a surgical challenge. The clinical outcome of free vascularized fibula graft (VFG) reconstruction with locking plate for massive femur and tibia defects of more than 10 cm that were secondary to skeletal tumor resection is reported. Materials and Methods: Thirteen patients with a mean follow-up of 3.3 years were reviewed. Seven patients received vascularized fibula grafts in the femur and six in the tibia. The mean bony defect of the femur and tibia was more than 10 cm and the length of the grafted fibula was more than 15 cm. All defects were stabilized with long locking plates. Results: All patients were free of disease at final follow-up; All VFGs were transferred successfully. All patients had a successful outcome with bony union. Stress fractures of the grafted fibula had occurred but the locking plate stabilized the fracture and healed until the last follow-up. All patients were able to walk without a brace after a mean of 9 months postoperatively. Conclusion: VFG with locking plate is a reliable reconstructive procedure for massive femur and tibia defects.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.1
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• pp.131-148
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• 2010

Piglet health at weaning is compromised due to several stress factors. Following the ban of antibiotic growth promoters new alternatives are required to control these problems. This paper reviews the evidence available for the use of spray dried animal plasma (SDAP) as an alternative to antibiotics in weaning pigs. Data from 75 trials in 43 publications involving over 12,000 piglets (mean values) have been used to calculate the performance responses of piglets according to several factors including SDAP origin, protein source from the control diet being replaced, dose of inclusion, age and weight of the piglets at weaning, sanitary conditions and simultaneous use or not of medication. Although the use of SDAP of all origins results in positive responses, it appears that plasma from porcine origin has the highest efficacy. This could be explained by the specificity of its IgG against porcine pathogens. During the first week post-weaning the response to plasma appears to increase with the inclusion dose, although over the two-week pre-starter period an optimal inclusion level of 4-8% is suggested. SDAP improves feed efficiency more markedly when the piglets are challenged with an experimental infection or when feed does not contain medication, which could be indicative of a lower expenditure of energy and nutrients to build an immune response against the challenge. There is evidence supporting that SDAP IgG and other bioactive substances therein prevent the binding of pathogens to the gut wall and reduce the incidence of diarrhoea in the post-weaning phase. Overall, plasma can be postulated as an excellent alternative to in-feed antimicrobials for piglets in the post-weaning phase.

• Advances in nano research
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• v.3 no.3
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• pp.143-168
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• 2015

Initiation of crack and its growth simulation requires accurate model of traction - separation law. Accurate modeling of traction-separation law remains always a great challenge. Atomistic simulations based prediction has great potential in arriving at accurate traction-separation law. The present paper is aimed at establishing a method to address the above problem. A method for traction-separation law prediction via utilizing atomistic simulations data has been proposed. In this direction, firstly, a simpler approach of common neighbor analysis (CNA) for the prediction of crack growth has been proposed and results have been compared with previously used approach of threshold potential energy. Next, a scheme for prediction of crack speed has been demonstrated based on the stable crack growth criteria. Also, an algorithm has been proposed that utilizes a variable relaxation time period for the computation of crack growth, accurate stress behavior, and traction-separation atomistic law. An understanding has been established for the generation of smoother traction-separation law (including the effect of free surface) from a huge amount of raw atomistic data. A new curve fit has also been proposed for predicting traction-separation data generated from the molecular dynamics simulations. The proposed traction-separation law has also been compared with the polynomial and exponential model used earlier for the prediction of traction-separation law for the bulk materials.