• 제목/요약/키워드: Cervus elaphus

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Cervus elaphus 종의 sequencing과 BLAST search에 의한 감별 (Identification of Cervus elaphus Species by Sequencing Analysis and BLAST Search)

  • 서정철;김민정;이찬;임강현
    • 대한본초학회지
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    • 제21권2호
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    • pp.129-133
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    • 2006
  • Objectives : Cervus elaphus species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Cenvus elaphus species could be identified by sequencing analysis and to verify Basic Local Alignment Search Tool (BLAST) search, which was used to assess genetic identification. Methods : The DNAs of Cervus elaphus species were extracted, amplified by PCR, and sequenced. The DNAs of Cervus species were identified by BLAST search in website. Results : By BLAST search one of Cervus elaphus species was identified as Cervus elaphussibericus but the other was identified as Cervus elaphus nelsoni. This work showed that identification can efficiently be performed by BLAST search. Conclusion : These results suggest that sequencing following BLAST search might be able to provide the identification of Cervus elaphus species.

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녹용(鹿茸) 및 녹용약침(鹿茸藥鍼)이 동물(動物)의 성장(成長)과 지능발달(知能發達)에 미치는 영향(影響) (The Effects of Cervus elaphus on the Growth and the Intellectual Development of Animals)

  • 김영태;손양선;진수희;한상원;심인섭;임사비나;이학인
    • Journal of Acupuncture Research
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    • 제18권5호
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    • pp.122-134
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    • 2001
  • The effects of Cervus elaphus and Cervus elaphus aquapuncuture on body weight, protein efficiency ratio, body length, serum growth hormone and intellectual development were studied for thirty-four days. The results were summarized as follows. 1. Body weight significantly increased in Cervus elaphus aquapuncture oral administration group compared to GH groups. 2. Protein efficiency ratio had no significant difference within all groups. 3. Body length significantly increased in Cervus elaphus aquapuncture group compared to GH injection group on 3rd day, tail length significantly increased in Cervus elaphus aquapuncture group and Cervus elaphus aquapuncture oral administration group compared to GH injection group but, body length has no significant difference within all groups. 4. Serum GH significantly increased in Cervus elaphus aquapuncture oral administration group compared to that of GH injection group. 5. As results of observing memory acquisition using Morris water maze system, there was no significant difference within all groups. 6. As results of observing retention using Morris water maze system, staying times significantly increased in Cervus elaphus aquapuncture oral administration group compared to that of GH injection group at Ist trial and 3rd trial. 7. As results of observing staining intensity of NADPH-d-positive neurons in tissue of hippocampal part, significant increasing of staining intensity were observed in septum and VDB of hippocampus in Cervus elaphus aquapuncture oral administration group compared to that of GH injection group. According to the above results, it is concluded that Cervus elaphus oral administration and Cervus elaphus aquapuncture on acupoint G39 showed effects on growth and intellectual development of animals.

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녹용수침자극(鹿茸水鍼刺戟)이 명문화쇠형(命門火衰型) 양위(陽萎)에 미치는 영향(影響) (A Study of the Effect by Cervus Elaphus Aqua-Acupuncture on the Hypogonadism induced by Hydrocortisone Acetate in Rats)

  • 윤종화;김갑성
    • 동국한의학연구소논문집
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    • 제2권1호
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    • pp.55-71
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    • 1993
  • In order to investigate the effect of Cervus elaphus aqua-acupuncture on the hypogonadism in male rats, hydrocortisone acetate was adminsterd per mouse to induce hypogonadism and a series of Cervus elaphus aqua-acupuncture and normal saline aqua-acupuncture treatments were done into the loci corresponding to Shinsu($BL_{23}$) for 10 days. The level of urinary 17-ketosteroids. serum FSH, prolactin, LH, testosterone and body weight were measured. The obtained results are summarized as follows. 1. The level of urinary 17-ketosteroids was increased by Cervus elaphus aqua-acupuncture with the statistical significance as compared with the control group. 2. The level of serum FSH was increased by Cervus elaphus aqua-acupuncture with the statistical significance as compared with the control group. 3. The level of serum prolactin was increased by Cervus elaphus aqua-acupuncture with the statistical significance as compared with the control group. 4. The level of serum LH was increased by Cervus elaphus aqua-acupuncture with the statistical significance as compared with the control group. 5. The level of serum testosterone was decreased by Cervus elaphus aqua-acupuncture with the statistical significance as compared with the control group. 6. Body weight change was increased by 3rd, 6th and 9th day after the Cervus elaphus aqua-acupuncture and 3rd,6th day after normal saline aqua-acupuncture with the statistical significance as compared with the control group.

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녹용추출액과 노제의 세포증식 및 면역활성도 비교 연구 (Comparison Study on the Cell Increase and Immunity Activation Degree in The extracted liquid of Cervus Elaphus Xanthopgus and It's Distilled Liquid)

  • 김광중;안상우;송효인
    • 동의생리병리학회지
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    • 제18권1호
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    • pp.148-156
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    • 2004
  • In order to searched for the ways of use for distilled liquid of Cervus Elaphus Xanthopgus, the study examined the difference of immunological efficiency generally raised in the existing extracted liquid and distilled liquid of Cervus Elaphus Xanthopgus. As seen from the above results, both the extracted liquid of Cervus Elaphus Xanthopgus and distilled liquid have remarkable efficiency in the increase of cells, the efficient condition of immunity activation degree, the effect of anti-cancer possibly formed by these two and activation of cells and organism, in which distilled liquid in the beginning and the extracted liquid later tend to show its efficiency.

녹용약침으로 치료한 De Quervain's Disease 4례 증례보고 (The clinical observations of 4 case of De Quervain's Disease treated with Cervus elaphus Herbal-acupuncture)

  • 박준성;김우영;백승태;이승덕;김갑성
    • Journal of Acupuncture Research
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    • 제21권6호
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    • pp.259-267
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    • 2004
  • De Quervain's disease is caused by stenosing tenosynovitis of the first dorsal compartment of the wrist. The first dorsal compartment at the wrist includes the tendons of the abductor pollicis longus (APL) and the extensor pollicis brevis (EPB). Patients with De Quervain's usually report pain at the dorsolateral aspect of the wrist with referral of pain toward the thumb and/or lateral forearm. This condition responds well to nonsurgical treatment. Objective : The aim of this study was to find out the therapeutic effect of the Cervus elaphus Herbal-acupuncture in the treatment of patients with De Quervain's Disease. Design : A prospective study of Cervus elaphus Herbal-acupuncture was conducted. Setting : The study was conducted in the Kang-nam Oriental Hosp. of Dong-Guk University. Patients : The study comprised 4 patients with De Quervain's Disease. Methods : We investigated 4 cases patients the De Quervain's Disease. We evaluated wrist function that before and after Cervus elaphus Herbal-acupuncture treatment by VAS score, tenderness and Finkelstein test. the treatment is operated every other day. Results : Pain reduction(VAS) was found out in the case treated with Cervus elaphus Herbal-acupuncture. Conclusions : Clinical results show that Cervus elaphus Herbal-acupuncture has a good analgesic effect in De Quervain's Disease.

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Discrimination of velvet antlers' origin using DNA polymorphisms

  • Chung, Hwan-Suck;Lee, Hye-Jeong;Kim, Young-Eun;Shin, Min-Kyu;Hong, Moo-Chang;Kim, Yang-Seok;Bae, Hyun-Su
    • Journal of Evidence-Based Herbal Medicine
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    • 제2권1호
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    • pp.7-12
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    • 2009
  • Velvet antlers from Cervus elaphus species are one of most famous, expensive and commonly used medicinal materials in traditional oriental medicine. Some distributor had illegal practice of disguising the origin of antlers in Korea market. Therefore, a test to distinguish antler essential to ensure the healthy development of the herbal industry. In this study, the variation in DNA sequences of the mitochondrial ATPase8 and cytochrome-coxidaseI (COI) genes of Cervus elaphus from China, the Republic of Altai, and Canada were evaluated. In addition, the sequence variation among, Rein deer and Cervus elaphus species was also evaluated. Although the sequences of deer from the Republic of Altai and Canada were very similar, polymorphisms that were conserved in each species were observed in the ATPase8 and COI genes. Therefore, these polymorphic markers could be used to distinguish Cervus elaphus antlers from different locations.

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녹용 약침이 국소 뇌허혈 모델 백서에서 방사형 미로과제 학습 및 뇌신경 보호에 미치는 영향 (Effect of Cervus Elaphus-Herbal Acupuncture on Focal Ischemia Induced by Inserted Intraluminal Filament in MCA of Rats)

  • 김성옥;윤대환;나창수
    • Korean Journal of Acupuncture
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    • 제22권4호
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    • pp.67-81
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    • 2005
  • Objectives : Cervus Elaphus(CE) has been used to medication for ischemic stroke in the Oriental Medicine. So this study was planned to investigate the effects of CE herbal acupuncture therapy(CE-HAT) on the focal ischemia-induced by intraluminal filament insertion in the rats. Materials and methods : The focal ischemia was induced by middle cerebral artery occlusion for 2hours. The groups divided into 6 groups, normal(intachess group), control(no theray group after ischemia-induced), CE-HAT1(Cervus Elaphus-herbal acupuncture therapy group at LR1, HT9 after ischemia-induced), CE-HAT2(Cervus Elaphus-herbal acupuncture therapy group at LU8, KI7 after ischemia-induced), CE-HAT3(Cervus Elaphus-herbal acupuncture therapy group at LR1, HT9, LU8, KI7 after ischemia-induced), CE-HAT4(Cervus Elaphus-herbal acupuncture therapy group at LR1, HT9, HT3, KI10 after ischemia-induced). CE-HAT was carried out during 3 weeks after focal ischemia-induced. Eight-arm radial maze was used for the behavioral task and neuropretective effect of CE-HAT was observed by Cresyl violet, AchE, ChAT-stain. Results : The error rate in the eight-arm radial maze task was significantly decreased in CE-HAT1, CE-HAT2, CE-HAT4 on 3days, CE-HAT4 on 4days, CE-HAT2, CE-HAT4 on 5days, CE-HAT3, CE-HAT4 on 6days. The rate of correct choice was significantly increased in CE-HAT4. The density of neurons in the hippocampal CA1 was significantly increased in CE-HAT1, CE-HAT2, CE-HAT3, CE-HAT4, compared to control group. The density of ChAT in the hippocampal CA1 was significantly increase in CE-HAT4. The density of ChAT in the hippocampal CA1 was significantly increased CE-HAT1. Conrlusions : These results suggest that the Cervus Elaphus-herbal acupuncture therapy could be used as a medication for controlling the stroke induced by deficiency.

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녹용약침과 인삼약침이 동물의 성장에 미치는 영향 (The Effects of Cervus elaphus Aquapuncture and Ginseng Radix Aquapuncture on the Growth of Animals)

  • 이진만;김영태;이학인;손양선;진수희;이향숙;박히준;이혜정;임사비나
    • 대한약침학회지
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    • 제3권2호
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    • pp.131-152
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    • 2000
  • The effects of Cervus elaphus aquapuncuture and Ginseng Radix aquapuncuture on the growth, protein efficiency ratio, contents of total protein, total cholesterol, and triglyceride in serum were studied for thirty four days. The results were summarized as follows. 1. Weight significantly increased in $0.3\%$ Cervus elaphus aquapuncture group on Chunchu loci and in $0.2\%$ Ginseng Radix aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 2. Protein efficiency ratio significantly increased in $0.2\%$ and $0.3\%$ of Cervus elaphus aquapuncture group on Chunchu loci and $0.1\%$, $0.2\%$ and $0.3\%$ of Ginseng Radix aquapuncture group on Chunchu loci compared to nonnal saline aquapuncture on Chunchu loci. 3. Total protein significantly increased in $0.2\%$ and $0.3\%$ of Cervus elaphus aquapuncture group on Chunchu loci compared to nonnal saline aqriapuncture On Chunchu loci. whereas it had a tendency to increase in $0.2\%$ Ginseng Radix aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 4. Total cholesterol increased a little in $0.3\%$ Cervus elaphus aquapuncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. 5. Triglyceride significantly increased in $0.2\%$ Ginseng Radix aqua puncture group on Chunchu loci compared to normal saline aquapuncture group on Chunchu loci. Significance was acknowledged in the effect by density difference of medicines. In the Cervus elaphus group, there was the most significant increase in density of $0.3\%$ aquapuncture, whereas in Ginseng Radix group, there was the most significant increase in density of $0.2\%$ aquapuncture. So setting up density of medicines is important and many various studies for clinical application are required.

미토콘드리아 displacement loop 영역의 염기서열을 이용한 녹용의 원산지 동정 (Molecular Identification of Deer Antlers using Nucleotide Sequences of Mitochondrial Displacement Loop Region)

  • 유현숙;이기남;이진성
    • 생명과학회지
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    • 제20권12호
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    • pp.1859-1866
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    • 2010
  • 우리나라는 세계 녹용 생산량의 80% 이상을 소비한다. 하지만, 중국산, 뉴질랜드산 녹용과 수입이 금지된 북미산 녹용이 원용이라고 불리는 고가의 러시아산 녹용으로 둔갑 판매, 유통되는 문제가 다수 보고되고 있다. 따라서 본 연구에서는 녹용의 원산지 동정 기술을 개발하고자 러시아, 중국, 뉴질랜드 및 북미산 녹용으로부터 미토콘드리아 D-loop 영역에 대한 원산지 특이적인 분자 마커를 탐색할 목적으로 수행되었다. 결과적으로 중국산 녹용으로부터 약 60~70 bp의 결실 부위를 확인하고 이들 부위를 특이적으로 확인할 수 있는 PCR법을 통해서 중국산 녹용의 정확한 감별 가능성을 확인하였다. 따라서 본 연구는 미토콘드리아 DNA 유래의 유전자들에 대한 염기서열 분석과 이를 이용한 PCR 동정법이 녹용의 원산지 감별에 적용될 수 있음을 보여주는 사례라 생각된다.

허혈(虛血)/재관류(再貫流) 뇌(腦) 조직(組織)에서 녹용(鹿茸) 약침액(藥鍼液)의 항산화작용(抗酸化作用)에 대한 연구(硏究) (Effects of Cervus elaphus extract solution for Aqua-acupuncture on Antioxidation in Rat's Brain induced by ischemia and reflow)

  • 정지천
    • 대한한방내과학회지
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    • 제20권1호
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    • pp.167-180
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    • 1999
  • Cervus elaphus, being known to reinforce Kidney, have tested to study the effects concerning damages of brain tissue induced by lipid peroxidation. In vitro, the level of lipid peroxide in brain tissue was decreased proportinally according to dose by Cervus elaphus extract solution for aqua-acupuncture (CESAA). It was much more decreased, when lipid peroxidation was induced with Fe(II). And, it was seen proportinally decrease according to the dose of CESAA on xanthine oxidase activities and type conversion ratio. However, I can not find special changes about aldehyde oxidase activities. And, I had observed the effects of CESAA on damages of rat's brain following ischemia and reflow. Before ischemia was caused, CESAA was applied 0.2 ml per 250 g through femoral vein in ischemia and reflow group and normal sailine was applied in normal group. Ischemia was caused by cervical artery's clamp for 30 min and reflowed by clamp remove after 15 min. It was increased on the content of lipid peroxidation, activies and type conversion ratio of xanthine oxidase following ischemia and reflow. However, they were decreased when CESAA was pre-appllied. However, it could not seen special changes on aldehyde oxidase activities, either. In conclusion. CESAA recovers the damage of brain due to ischemia and reflow by decreasing the lipid peroxidation through decreasing of xanthine oxidase activies and type conversion ratio.

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