• Clinical and Experimental Reproductive Medicine
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• v.49 no.1
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• pp.49-56
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• 2022

Objective: Oxidative stress and sperm DNA fragmentation (SDF) have been linked to idiopathic male infertility (IMI). Various antioxidants have been tried to improve semen parameters and fertility potential in IMI patients, but with inconsistent results. The study aimed to compare the effects of coenzyme Q10 (CoQ10) and Centrum multivitamins on semen parameters, seminal antioxidant capacity, and SDF in infertile men with idiopathic oligoasthenospermia (OA). Methods: This prospective controlled clinical study involved 130 patients with idiopathic OA and 58 fertile controls. The patients were divided randomly into two groups: the first group received CoQ10 (200 mg/day orally) and the second group received Centrum multivitamins (1 tablet/day) for 3 months. Semen parameters, CoQ10 levels, reactive oxygen species (ROS), total antioxidant capacity (TAC), catalase, SDF, and serum hormone levels (follicle-stimulating hormone, luteinizing hormone, testosterone, and prolactin) were compared at baseline and after 3 months. Results: Both CoQ10 and Centrum improved sperm concentration and motility, but the improvement was greater with Centrum therapy (p<0.05). Similarly, both therapies improved antioxidant capacity, but TAC and catalase improvement was greater (p<0.01 and p<0.001 respectively) with CoQ10, whereas ROS (p<0.01) and SDF (p<0.001) improvements were greater with Centrum administration. Centrum therapy was associated with reduced serum testosterone (p<0.05). Conclusion: In conclusion, both CoQ10 and Centrum were effective in improving semen parameters, antioxidant capacity, and SDF, but the improvement was greater with Centrum than with CoQ10. Therefore, Centrum-as a source of combined antioxidants-may provide more effective results than individual antioxidants such as CoQ10 in the treatment of infertile men with idiopathic OA.

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.189-198
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• 1993

The present study was undertaken to provide basic data on endochondral ossification for the axis in developing Miniature Schnauzers. This study was determined to the morphological features and development of growth plast in the axis of this experimental animals by histological and histochemical methods. The axis from 2 healthy Miniature Schnauzers(postnatal 6hr, 5week) was used. The obtained results were as follows : 1. In 5-week-old Miniature Schnauzer, the axis consisted of 4 separate ossification centers : centrum l, intercentrum 2, centrum 2 and epiphysis. Intercentrum 2 was intercalated between centrum 1 cranially, centrum 2 caudally. 2. The space of centrum 1 was more broader than the other ossification centers. 3. The zone of reserved chondrocytes was more extensive than the zone of proliferative chondrocytes, trabeculation was weakly observed, however, the proximal epiphyseal plate of axis was actively trabeculation observed in the zone of calcified chondrocytes. 4. Eighteen columns of chondrocytes were observed in the centrum 1 and five to seven columns of chondrocytes were observed in the centrum 2 of Miniature Schnauzer(postnatal 5 week) 5. A positive reaction to alcianophility was observed in all the territorial matrix at the zone of hypertrophic chondrocytes in this experimental animals.

• Investigative Magnetic Resonance Imaging
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• v.23 no.3
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• pp.241-250
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• 2019

Purpose: The purpose of this study was to determine the relation between quantitative magnetic resonance imaging biomarkers, and clinical performances in chronic phase of carbon monoxide intoxication. Materials and Methods: Eighteen magnetic resonance scans and cognitive evaluations were performed, on patients with carbon monoxide intoxication in chronic phase. Apparent diffusion coefficient (ADC) ratios of affected versus unaffected centrum semiovale, and corpus callosum were obtained. Signal intensity (SI) ratios between affected centrum semiovale, and normal pons in T2-FLAIR (fluid-attenuated inversion recovery) images were obtained. The Mini-Mental State Exam, and clinical outcome scores were assessed. Correlation coefficients were calculated, between MRI and clinical markers. Patients were further classified into poor-outcome and good-outcome groups based on clinical performance, and imaging parameters were compared. T2-SI ratio of centrum semiovale was compared, with that of 18 sex-matched and age-matched controls. Results: T2-SI ratio of centrum semiovale was significantly higher in the poor-outcome group, than that in the good-outcome group and was strongly inversely correlated, with results from the Mini-Mental State Exam. ADC ratios of centrum semiovale were significantly lower in the poor outcome group than in the good outcome group, and were moderately correlated with the Mini-Mental State Exam score. Conclusion: A higher T2-SI and a lower ratio of ADC values in the centrum semiovale, may indicate presence of more severe white matter injury and clinical impairment. T2-SI ratio and ADC values in the centrum semiovale, are useful quantitative imaging biomarkers for correlation with clinical performance in individuals with carbon monoxide intoxication.

• Korean Journal of Veterinary Research
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• v.35 no.1
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• pp.19-28
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• 1995

The histological and histochemical study of the axis body of piglet was performed in order to clarify the ossification process. These experimental animals collected from after birth to 52 days were used for this study. The results were summarized as follows; 1. The epiphysis, the intercentrum 2 and the centrum of proatlas were appeared in the axis body and dens of piglet after birth. 2. In 39 days of age piglet, the ossification of the intercentrum 2 was first observed but the epiphysis was already initiated. The centrum of proatlas was observed cranioventral part of the dens of the 52 days piglet axis. 3. The notochordal remnants revealed distinctively in the part between the centrum 1 and the centrum2, in the apical cartilaginous tissue of dens and in the caudal cartilaginous part of body of axis. 4. Intramembranous ossification occurred in the adjacent area of perichondrium of centrum 2. These ossified trabeculae show the woven bone configuration.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.5
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• pp.385-399
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• 2005

The phenotypic response of a cell results from a well orchestrated web of complex interactions which propagate from the genetic architecture through the metabolic flux network. To rationally design cell factories which carry out specific functional objectives by controlling this hierarchical system is a challenge. Transcriptome analysis, the most mature high-throughput measurement technology, has been readily applied In strain improvement programs in an attempt to Identify genes involved in expressing a given phenotype. Unfortunately, while differentially expressed genes may provide targets for metabolic engineering, phenotypic responses are often not directly linked to transcriptional patterns, This limits the application of genome-wide transcriptional analysis for the design of cell factories. However, improved tools for integrating transcriptional data with other high-throughput measurements and known biological interactions are emerging. These tools hold significant promise for providing the framework to comprehensively dissect the regulatory mechanisms that identify the cellular control mechanisms and lead to more effective strategies to rewire the cellular control elements for metabolic engineering.

• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.59-69
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• 1997

The histological and histochemical study of the body of axis and 1st intervertebral disc in the Korean native cattle fetus was attempted to early developmental process. The experimental animals used in this study were collected from fetus of the Korean native cattle ranging from 50mm to 180mm in Crown-Rump length(CR-length). The results were summerized as follows: 1. The ossification centers appeared centrum 1 and centrum 2 of the axis in 110mm CR-length fetus of the Korean native cattle. The centrum 2 was formed little earlier than the centrum 1. 2. The histochemical reactions for collagenous fibers in the axis revealed negative in 50mm CR-length, mild positive in 90mm CR-length, and strong positive in 110mm CR-length, respectively. 3. Dense collagenous fibers were observed in the notochord through the centrum 2, and intervertebral disc developed into cuneiform dorsoventrally, It's contour looks like an annual ring. These fiber bundle arranged lamellar formation. 4. The intervertabral disc of 50mm CR-length fetus was composed mainly mesenchymal cells, and these cell showed aggregation in the central portion. The intervertebral disc of 110mm CR-length fetus was consisted of pricipally fibroblast, and notochordal sheath formed with two layers in the center.

• Proceedings of the Ginseng society Conference
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• 1978.09a
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• pp.41-42
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• 1978

• Molecules and Cells
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• v.37 no.3
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• pp.189-195
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• 2014

The NF-${\kappa}B$ pathway transcriptionally controls a large set of target genes that play important roles in cell survival, inflammation, and immune responses. While many studies showed anti-tumorigenic and pro-survival role of NF-${\kappa}B$ in cancer cells, recent findings postulate that NF-${\kappa}B$ participates in a senescence-associated cytokine response, thereby suggesting a tumor restraining role of NF-${\kappa}B$. In this review, we discuss implications of the NF-${\kappa}B$ signaling pathway in cancer. Particularly, we emphasize the connection of NF-${\kappa}B$ with cellular senescence as a response to chemotherapy, and furthermore, present examples how distinct oncogenic network contexts surrounding NF-${\kappa}B$ produce fundamentally different treatment outcomes in aggressive B-cell lymphomas as an example.

• Journal of the Korean earth science society
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• v.37 no.1
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• pp.1-10
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• 2016

Four entities of the Cenozoic fish fossils were discovered in the Miocene Duho Formation, Pohang, Korea. these fossils were identified as the first Pleuronectiformes in Korea based on the following-the presence of postcleithrum, the elongation of the first proximal pterygiophore of the anal fin ray, almost consistent presence of two proximal pterygiophore of the anal fin rays between the two adjacent hemal spines, the fusion of the first and second hypurals, the fusion of the third and fourth hypurals and the first preural centrum, the presence of well-developed anteriormost plate-like neural spine, the presence of the urohyal like fish-hook and its elongated sciatic part, and the division of the parahypural from the first preural centrum. On the other hand, geological studies about the Duho Formation consistently claimed that shallow-sea creatures were washed away by meteorological events such as a great flood and deposited at the bottom of deep-sea by the turbidity current. However, in Duho Formation, only shallow-sea ones have been discovered thus far. This study reported that Flat fish, deep-sea creature, was discovered in Duho Formation for the first time in Korea.

• 한국해양학회지
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• v.5 no.1
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• pp.37-40
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• 1970

This work concerns age studies of the spanish mackerel by vertebrae. The spanish mackerel samples were obtained mainly from Yosoo and Inchon regions during July to December, 1969. A total of 292 fish was collected during the present study. Except the vertebrae, the incremental growth is found to be vague or complicated on the most bony parts in spanish mackerel. The spanish mackerel has 50 vertebrae, and the size of vertebra varies according to its position. After several studies, therefore, sample centrum was selected from the 23rd vertebra throughout the present study. Sample centrum was then stained with Alizarin Red S. and examined under binocular stereoscopic microscope illuminated by reflected light. The surface of the centrum shows many ring-marks running parallel to the edge. The ring-marks were counted and the measurements of centrum radius (R) and radii of the ring-marks (r$\_$i/) were made by eyepiece micrometer. From available data, the average of each ring-mark radius was found to be; r$\_$1/=2.05mm, r$\_$2/=3.11mm, r$\_$3/=4.05mm, r$\_$4/=4.89mm, r$\_$5/=5.70mm and r$\_$6/=6.47mm, respectively. The mean fork length at the time of ring-mark formation was calculated to be; l$\_$1/=297.6mm, l$\_$2/= 390.1mm, l$\_$3/=472.2mm, l$\_$4/=545.5mm, l$\_$5/=616.2mm, and l$\_$6/= 683.4mm, respectively.