• 한국정보통신학회논문지
• /
• 제9권7호
• /
• pp.1412-1418
• /
• 2005

현재 점점 더 많은 휴대용 단말들은 서로 다른 access 기술들을 이용하여 Internet에 연결하기 위해 많은 인터페이스를 가지고 있다. 이러한 각각의 access 기술들은 서비스 지윈 영역, bandwidth, 그리고 신뢰성 등의 그들만의 특성들을 갖게 된다. 이와 같이 두 개 이상의 인터페이스를 가지고 있는 단말은 현재 사용 중인 인터페이스의 문제가 발생했을 경우 다른 여분의 인터페이스를 통해 인터넷에 접하거나, 효과적으로 traffic을 분산시킬 수 있는 등 많은 장점들을 지니고 있다. 이런 환경을 단말의 Multihoming이라고 하는데 현재까지는 인터넷 상의 프로토콜이 이와 같은 Multihoming을 지원하고 있지 않다. 따라서 본 논문은 이와 같은 IPv6 환경에서 이동 단말의 Multihoming 지원 시 필요한 기술적인 고려사항에 대해 알아보고 multiple interface로부터 획득한 address들의 등록을 통해 신뢰성 있는 접근성과 vertical handover를 지원할 수 있는 방법을 소개한다.

• 정보처리학회논문지D
• /
• 제11D권5호
• /
• pp.1149-1158
• /
• 2004

최근 다양한 무선 단말기의 보급과 네트워크 기술의 발전으로 인하여 무선 단말기를 이용한 인터넷 접속이 보편화되고 있다. 특히 디지털 방송의 도입에 따른 다양한 방송 프로그램과 방송사 뉴스 서비스는 무선 단말기의 제한된 환경에서의 이용률이 높다. 그러나 대부분의 방송사 웹 페이지들은 한 페이지에 많은 내용으로 인한 세분화된 섹션을 담고 있기 때문에 제한된 화면과 입력장치를 가진 무선 단말기를 이용하여 사용자가 원하는 부분에 접근하기까지 반복적인 스크롤링을 해야 하는 불편함이 있다. 이러한 문제를 해결하기 위해 본 논문에서는 방송사 웹페이지 내에서 실시간으로 사용자가 선호하는 방송사 웹 페이지의 섹션을 추출하고, 무선 환경에 적합하도록 각 섹션의 순서를 재구성하여 무선 단말기에 제공해 주는 기법을 제안한다. 제안된 기법을 통해 사용자는 무선 단말기의 단점을 극복함과 동시에 방송사 웹에서 선호하는 섹션의 맞춤형 방송사 웹 서비스를 제공받을 수 있다.

• IMMUNE NETWORK
• /
• 제5권3호
• /
• pp.157-162
• /
• 2005

Background: 1-8D gene is a member of human 1-8 interferon inducible gene family and was shown to be overexpressed in fresh colon cancer tissues. Three peptides 1-6, 3-5 and 3-7 derived from human 1-8D gene were shown to have immunogenicity against colon cancer. Methods: To study tumor immunotherapy, of three peptides we established an active immunization model using HHD mice. $D^{b-/-}{\times}{\beta}2$ microglobulin $({\beta}2m)$ null mice transgenic for a chimeric HLA-$A2.1/D^{b-}\;{\beta}2m$ single chain (HHD mice) were challenged with B16/HHD/1-8D tumor cells and were immunized with irradiated peptide-loaded RMA- S/HHD/B7.1 transfectants. In therapy model tumor growth was retarded in HHD mice that were injected with 3-5 peptide-loaded RMA-S/HHD/B7.1. In survival test vaccination with 1-8D-derived peptide protects HHD mice from tumor progression after tumor challenge. Results: These studies show that peptide 3-5 derived from 1-8D gene can be the most effective candidate for the vaccine of immunotherapy against colon cancer and highlight 1-8D gene as putative colon carcinoma associated antigens. Conclusion: We demonstrated that RMA-S/HHD/ B7.1 loaded with 1-8D peptides, especially 3-5, immunization generates potent antitumor immunity against tumor cells in HHD mice and designed active immunization as proper immunotherapeutic protocols.

• IMMUNE NETWORK
• /
• 제4권2호
• /
• pp.81-87
• /
• 2004

Background: In the thymus, developing thymocytes continually interact with thymic epithelial cell components. Self MHC restriction of mature T cells are imposed in the thymus through interaction of immature double positive thymocytes and thymic cortical epithelial cells. The site of negative selection, however, is a matter of debate. Through systemic injection of anti-TCR antibody or antigenic peptides, investigators suggested that most of the negative selection occurs in the thymic cortex. But the requirements for negative selection, i.e cellular counterparts and costimulatory molecules are more available in the medulla or cortico-medullary junction rather than in the thymic cortex. Methods: The direct and indirect pathways of thymocyte death after systemic anti-TCR antibody injection were separated through several experimental systems. B6 mice were either adrenalectomized or sham-adrenalectomized to evaluate the role of endogenous glucocorticoids from adrenal gland. Role of TNF were evaluated through using TNF receptor double knockout mice. Results: We found that without indirectly acting mediators such as $TNF-\alpha$ or corticosteroid, double positive thymocyte death were minimal by systemic injection of anti-TCR antibody in TNF receptor double knockout neonatal mice. Also by analyzing neonatal wild-type mice with adoptively transferred mature T cells, only peripheral activation of mature T cells could induce extensive double positive thymocyte death. Conclusion: Thus, systemically injected anti-TCR antibody mediated thymocyte death are mostly induced through indirect pathway.

• IMMUNE NETWORK
• /
• 제3권4호
• /
• pp.276-280
• /
• 2003

Background: Peroxidases (Prx) of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol respectively. Hydrogen peroxide is implicated as an intracellular messenger in various cellular responses such as proliferation and differentiation. And Prx I activity is regulated by Cdc-2 mediated phosphorylation. This work was undertaken to investigate the proliferation role of peroxiredoxin III as a member of Prx family in Prx III overexpressed HeLa cell line. Methods: To provide further evidence of proliferation, we selected Prx III stably expressed HeLa Tet-off cell lines. Cell proliferation was examined by using proliferation reagent WST-1 in the presence or absence of doxycycline. Prx III, 2-cys Prx enzymes exist as homodimer. The activation of Prx III heterodimer with induced and endogenous Prx III was examined by immunoprecipitation. Results: Immunoprecipitation analysis of the induced and endogenous Prx III with anti-myc showed that the induced wild type (WT) and dominant negative (DN) Prx III from HeLa Prx III Tet-off stable cell heterodimerized with endogenous Prx III each other. And the expression level of induced Prx III was examined after addition of doxycycline. By 72 hr, the expression level of induced Prx III was diminished gradually and the half-life of the induced wild type Prx III was approximately 17 hr. The proliferation experiment demonstrated that the relative proliferation value of induced and endogenous WT Prx III stable cell has no changes but the DN Prx III induced HeLa Tet-off stable cells were lower than endogenous Prx III. Conclusion: In conclusion, the HeLa dominant negative Prx III Tet-off stable cells were decreased the proliferation.

• IMMUNE NETWORK
• /
• 제9권2호
• /
• pp.58-63
• /
• 2009

Background: T cell immunoglobulin and mucin domain containing 3 protein (Tim-3) expressed on terminally differentiated Th1 cells plays a suppressive role in Th1-mediated immune responses. Recently, it has been shown that N-glycosylation affects the binding activity of the Tim-3-Ig fusion protein to its ligand, galectin-9, but the binding properties of non-glycosylated Tim-3 on $CD4^+CD25^+$T cells has not been fully examined. In this study, we produced recombinant Tim-3-Ig fusion proteins in different cellular sources and its N-glycosylation mutant forms to evaluate their binding activities to $CD4^+CD25^+$T cells. Methods: We isolated and cloned Tim-3 cDNA from BALB/C mouse splenocytes. Then, we constructed a mammalian expression vector and a prokaryotic expression vector for the Tim-3-Ig fusion protein. Using a site directed mutagenesis method, plasmid vectors for Tim-3-Ig N-glycosylation mutant expression were produced. The recombinant protein was purified by protein A sepharose column chromatography. The binding activity of Tim-3-Ig fusion protein to $CD4^+CD25^+$T cells was analyzed using flow cytometry. Results: We found that the nonglycosylated Tim-3-Ig fusion proteins expressed in bacteria bound to $CD4^+CD25^+$T cells similarly to the glycosylated Tim-3-Ig protein produced in CHO cells. Further, three N-glycosylation mutant forms (N53Q, N100Q, N53/100Q) of Tim-3-Ig showed similar binding activities to those of wild type glycosylated Tim-3-Ig. Conclusion: Our results suggest that N-glycosylation of Tim-3 may not affect its binding activity to ligands expressed on $CD4^+CD25^+$T cells.

• IMMUNE NETWORK
• /
• 제7권4호
• /
• pp.173-178
• /
• 2007

Background: We studied the role for expression of CD40 and CD40L by CD4 and CD8 T cells in the generation of CD8 T cell response to minor histocompatibility antigen, H60. H60 is a cellular antigen to which CD8 responses require CD4 T cell help. Methods: CD40- or CD40L-deficient mice were adoptively transferred with normal CD4 or CD8 T cells or with memory CD4 or CD8 T cells, and were immunized with male H60 congenic splenocytes to induce CD8 T cell response to H60. Peripheral blood CD8 T cell from the immunized mice were stained with the H60 tetramer. Results: CD8 T cell response to H60 was not induced in both CD40- and CD40L-deficient mice. Adoptive transfer of $CD40^{+/+}$ CD8 T cells into CD40-deficient mice did not compensate the defect in inducing CD8 T cell response to H60, while the H60-specific CD8 T cells were activated in the CD40-deficient mice that were adoptively transferred with $CD40^{+/+}$ CD4 T cells. Adoptive transfer of $CD40L^{+/+}$ CD4 T cells into CD40L-deficient mice induced primary CD8 T cell response for H60 and the presence of $CD40L^{+/+}$ CD4 T cells was required even for memory CD8 T cells response to H60. Conclusion: Our results suggest that the CD40-CD40L interaction mediates the delivery of CD4 T cell help to naive and memory H60-specific CD8 T cells. While the expression of CD40L by CD4 T cells is essential, signaling through CD40 on CD8 T cells is not required for the induction of CD8 T cell response to H60.

• IMMUNE NETWORK
• /
• 제7권4호
• /
• pp.197-202
• /
• 2007

Background: Immunomodulators enhancing MHC-restricted antigen presentation would affect many cellular immune reactions mediated by T cells or T cell products. However, modulation of MHC-restricted antigen presentation has received little attention as a target for therapeutic immunoregulation. Here, we report that lectins isolated from mushroom Fomitella fraxinea enhance MHC-restricted exogenous antigen presentation in professional antigen presenting cells (APCs). Methods: Lectins, termed FFrL, were isolated from the carpophores of Fomitella fraxinea, and its effects on the class I and class II MHC-restricted presentation of exogenous ovalbumin (OVA) were examined in mouse dendritic cells (DCs) and mouse peritoneal macrophages. The effects of FFrL on the expression of total MHC molecules and the phagocytic activity were also examined in mouse DCs. Results: DCs cultured in the presence of FFrL overnight exhibited enhanced capacity in presenting exogenous OVA in association with class I and class II MHC molecules. FFrL increased slightly the total expression levels of both class I (H-$2K^b$) and class II (I-$A^b$) MHC molecules and the phagocytic activity of DCs. Antigen presentation-enhancing activity of FFrL was also observed in macrophages isolated from mouse peritoneum. Conclusion: Lectins isolated from the carpophores of Fomitella fraxinea increase MHC-restricted exogenous antigen presentation by enhancing intracellular processing events of phagocytosed antigens.

• 한국사이버테러정보전학회:학술대회논문집
• /
• 한국사이버테러정보전학회 2004년도 제1회 춘계학술발표대회
• /
• pp.103-108
• /
• 2004

현재 HLR system은 이동전화 망에서 지속적으로 변하는 개별 가입자의 위치 정보를 관리 한다. 이를 수행하기 위해, HLR database system은 table 관리 기능과 색인 관리 기능, 그리고 백업 관리 기능을 제공한다. 본 논문에서는, 이동 전화 번호를 위한 적절한 색인 기법으로서 이단계 색인 기법의 사용과, 단말번호를 위한 버켓 연결 해슁 기법을 제안한다. 이동 전화 번호(MDN)와 단말번호(ESN)는 HLR database system에서 key로 사용된다. 또한 HLR database transaction의 특성을 고려한 효율적인 백업 방법을 제안한다. 이단계 색인 기법은 기존의 T 트리 색인 기법보다 검색 속도와 기억 공간 사용 효율 측면에서 우수하다. 버켓 연결 해슁 기법은 기존의 변형된 선형 해슁 기법보다 삽입과 삭제 시의 오버헤드가 적다. 제안한 백업 방법에서는, 빈번한 위치 등록 기능 수행으로 인해 야기되는 성능 저하 문제를 해결하기 위해 두가지 종류의 갱신 플래그를 사용하였다. 아울러 위치 정보의 보안성 처리를 위한 HLR 데이터베이스 Scheme을 제안하였다.

• 한국전자통신학회논문지
• /
• 제6권6호
• /
• pp.909-914
• /
• 2011

오늘날 이동통신 기술의 발전으로 사람 또는 사물의 위치와 이동 상황을 활용한 위치기반 서비스가 활성화되고 있다. 본 논문에서는 GPS 와 CDMA 통신 기능이 있는 단말과 위치 제공 서버를 이용하여 공공 청소차량 운행 관리를 위한 사설 위치 추적 시스템을 개발하였다. 차량에 장착된 단말 장치는 GPS 신호를 수신하여 시간, 위도, 경도, 그리고 고도 정보를 포함한 위치를 계산하고 이 정보를 CDMA 셀룰라 통신망을 통해 서버에 전송한다. 서버는 이 데이터를 데이터베이스에 저장하고 이동 차량을 지도화면에 표현해줄 맵 서버 프로그램이 사용할 수 있게 해준다. 이 시스템을 공공 청소차 운행 관리 업무에 적용하여 차량의 실시간 작업 상황과 이동 경로를 파악하여 합리적인 작업 및 이동 노선을 제시하여 업무 효율을 높일 수 있다.