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Lectins Isolated from Mushroom Fomitella fraxinea Enhance MHC-restricted Exogenous Antigen Presentation  

Kim, Hyun-Jin (College of Pharmacy, Chungbuk National University)
Cho, Kyung-Mi (College of Pharmacy, Chungbuk National University)
Gerelchuluun, Turmunkh (College of Pharmacy, Chungbuk National University)
Lee, Ji-Seon (College of Pharmacy, Chungnam National University)
Chung, Kyeong-Soo (College of Pharmacy, Chungnam National University)
Lee, Chong-Kil (College of Pharmacy, Chungbuk National University)
Publication Information
IMMUNE NETWORK / v.7, no.4, 2007 , pp. 197-202 More about this Journal
Abstract
Background: Immunomodulators enhancing MHC-restricted antigen presentation would affect many cellular immune reactions mediated by T cells or T cell products. However, modulation of MHC-restricted antigen presentation has received little attention as a target for therapeutic immunoregulation. Here, we report that lectins isolated from mushroom Fomitella fraxinea enhance MHC-restricted exogenous antigen presentation in professional antigen presenting cells (APCs). Methods: Lectins, termed FFrL, were isolated from the carpophores of Fomitella fraxinea, and its effects on the class I and class II MHC-restricted presentation of exogenous ovalbumin (OVA) were examined in mouse dendritic cells (DCs) and mouse peritoneal macrophages. The effects of FFrL on the expression of total MHC molecules and the phagocytic activity were also examined in mouse DCs. Results: DCs cultured in the presence of FFrL overnight exhibited enhanced capacity in presenting exogenous OVA in association with class I and class II MHC molecules. FFrL increased slightly the total expression levels of both class I (H-$2K^b$) and class II (I-$A^b$) MHC molecules and the phagocytic activity of DCs. Antigen presentation-enhancing activity of FFrL was also observed in macrophages isolated from mouse peritoneum. Conclusion: Lectins isolated from the carpophores of Fomitella fraxinea increase MHC-restricted exogenous antigen presentation by enhancing intracellular processing events of phagocytosed antigens.
Keywords
Fomitella fraxinea; lectin; MHC-restricted antigen presentation; dendritic cell; macrophage;
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1 Bevan MJ: Cross-priming for a secondary cytotoxic response to minor H antigens with H-2 congenic cells which do not cross-react in the cytotoxic assay. J Exp Med 143;1283-1288, 1976   DOI   ScienceOn
2 Heath WR, Carbone FR: Cross-presentation, dendritic cells, tolerance and immunity. Annu Rev Immunol 19;47-64, 2001   DOI   ScienceOn
3 Yewdell JW, Haeryfar SM: Understanding presentation of viral antigens to CD8+ T cells in vivo: the key to rational vaccine design. Annu Rev Immunol 23;651-682, 2005   DOI   ScienceOn
4 Karttunen J, Sanderson S, Shastri N: Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens. Proc Natl Acad Sci 89;6020-6024, 1992
5 Lee JS: Purification and characterization of a lectin with potent antitumor and immunomodulatory activity from a Korean wild mushroom Formitella fraxinea. A PhD thesis. Graduate School of Pharmacy, Chungnam National University, 2005
6 Cammue B, Stinissen HM, Peumans WJ: A new type of cereal lectin from leaves of couch grass (Agropyrum repens). Eur J Biochem 148;315-322, 1985   DOI   ScienceOn
7 Wang H, Gao J, Ng TB: A new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom Pleurotus ostreatus. Biochem Biophys Res Commun 275;810-816, 2000   DOI   ScienceOn
8 Gerelchuluum T, Lee YH, Lee YR, Im SA, Park JS, Song S, Kim K, Lee CK: Dendritic cells process antigens encapsulated in a biodegradable polymer, poly (D,L-lactide-co-glycolide), via an alternate class I MHC processing pathway. Arch Pharm Res 30;1440-1446, 2007   과학기술학회마을   DOI   ScienceOn
9 She QB, Ng TB, Liu WK: A novel lectin with potent immunomodulatory activity isolated from both fruiting bodies and cultured mycelia of the edible mushroom Vovariella volvacea. Biochem Biophys Res Commun 247;106-111, 1998   DOI   ScienceOn
10 Sigal LJ, Crotty S, Andino R, Rock KL: Cytotoxic T-cell immunity to virus-infected non-haematopoietic cells requires presentation of exogenous antigen. Nature 398;77-80, 1999   DOI   ScienceOn
11 Avichezer D, Gilboa-Garber N: Antitumoral effects of Pseudomonas aeruginosa lectins on Lewis lung carcinoma cells cultured in vitro without and with murine splenocytes. Toxicon 29;1305-1313, 1991   DOI   ScienceOn
12 Heath WR, Carbone FR: Cross-presentation, dendritic cells, tolerance and immunity. Annu Rev Immunol 19;47-64, 2001   DOI   ScienceOn
13 Lee YR, Yang IH, Lee YH, Im SA, Song S, Li H, Han K, Kim K, Eo SK, Lee CK: Cyclosporin A and tacrolimus, but not rapamycin, inhibit MHC-restricted antigen presentation pathways in dendritic cells. Blood 105;3951-3955, 2005   DOI   ScienceOn
14 Sigal L, Rock KL: Bone marrow-derived antigen-presenting cells are required for the generation of cytotoxic T lymphocyte responses to virus and use transporter associated with antigen presentation (TAP)-dependent and -independent pathways of antigen presentation. J Exp Med 192;1143-1150, 2000   DOI
15 Goldstein IJ, Heys CE: The lectins: Carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem 35;127-340, 1978   DOI
16 Licastro F, Morini MC, Kretz O, Dirheimer G, Creppy EE, Stirpe F: Mitogenic activity and immunological properties of bolesatine, a lectin isolated from the mushroom Boletus satanas Lenz. Int J Biochem 25;789-792, 1993   DOI   ScienceOn
17 Huang AY, Golumbek P, Ahmadzadeh M, Jaffee E, Pardoll D, Levitsky H: Role of bone marrow-derived cells in presenting MHC class I-restricted tumor antigens. Science 264;961-965, 1994   DOI
18 Lee YH, Lee YR, Im SA, Park SI, Kim KH, Gerelchuluum T, Song S, Kim K, Lee CK: Calcineurin inhibitors block MHC-restricted antigen presentation in vivo. J Immunol 179; 5711-5716, 2007   DOI
19 Shen Z, Reznikoff G, Dranoff G, Rock KL: Cloned dendritic cells can present exogenous antigens on both MHC class I and class II molecules. J Immunol 158;2732-2730, 1997
20 Wang H, Ng TB, Liu Q: Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom Agrocybe cylindracea. Life Sci 70;877-885, 2002   DOI   ScienceOn
21 Harding CV, Song R: Phagocytic processing of exogenous particulate antigens by macrophages for presentation by class I MHC molecules. J Immunol 153;4925-4933, 1994   PUBMED
22 Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS, Lee CK: Acemannan purified from Aloe vera induces phenotypic and functional maturation of immature dendritic cells. Int Immunopharmacol 1;1275-1284, 2001   DOI   ScienceOn
23 Harding CV: Phagocytic processing of antigens for presentation by MHC molecules. Trend Cell Biol 5;105-108, 1995   DOI   ScienceOn
24 Carbone FR, Kurts C, Bennett SR, Miller JF, Heath W: Crosspresentation: a general mechanism for CTL immunity and tolerance. Immunol Today 19;368-373, 1998   DOI   ScienceOn
25 Harding CV, Collins DS, Kanagawa O, Unanue ER: Liposomeencapsulated antigens engender lysosomal processing for class II MHC presentation and cytosolic processing for class I presentation. J Immunol 147;2860-2863, 1991   PUBMED
26 Guermonprez P, Valladeau J, Zitvogel L, Thery C, Amigorena S: Antigen presentation and T cell stimulation by dendritic cells. Annu Rev Immunol 20;621-667, 2002   DOI   ScienceOn
27 Wang HX, Liu WK, Ng TB, Ooi VE, Chang ST: The immunomodulatory and antitumor activities of lectins from the mushroom Tricholoma mongolicum. Immunopharmacol 31;205-211, 1996   DOI   ScienceOn