• 한국동물학회지
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• 제23권2호
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• pp.77-88
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• 1980

도룡뇽 피부점액선의 미세구조를 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 피부점액선은 선체부와 분비관으로 구성되며, 선체부는 선상피세포와 근상피세포로 구성되었다. 2. 피부점액선의 선체부의 선상피세포는 명세포, 암세포, mitochondria-rich cell, endoplasmic reticulum-developed cell, secretory granule-containing cell 및 vesiculated cells들로 구성되었다. 3. 명세포, 암세포 및 mitochondria-rich cell은 미분화세포, endoplasmic reticulum-developed cell은 분비전기세포, secretory granule-containing cell은 성숙세포 그리고 vesiculated cell은 분비후기세포라고 생각된다.

• 대한안전경영과학회지
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• 제15권3호
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• pp.143-150
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• 2013

A cell site lease is a legal agreement by which the owner of a building or a piece of land allows a wireless carrier to use part of it for a cell tower for a period of time in return for money. Wireless carriers spend significant money for cell site leases. This paper tries to find factors affecting cell site leases and management solutions to save lease costs. In other words, this research identifies any factor influencing cell site leases among age, gender, and geographical area in the first problem and determines management priorities using an importance-satisfaction model in the second problem. This paper can provide wireless carriers with effective decision making tools and a basis for negotiation of cell site leases, as they do not have enough bases for negotiation.

• International Journal of Contents
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• 제8권3호
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• pp.100-104
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• 2012

In this paper, a unit cell for low pass filter design by using composite right and left-handed transmission line in conductor-backed coplanar waveguide is proposed. The characteristics of the unit cell are analyzed in order to design a low pass filter in small sizes. By changing the sizes of the unit cell, the parameters of right-handed and left-handed immittance components are changed and the desired characteristics of the unit cell are achieved. The equivalent circuit of the unit cell is extracted and analyzed either. As a result, the simulation results of the unit cell and the equivalent circuit are almost identified. The movement and energy distributions of electromagnetic field are shown to confirm the property of the unit cell. In the end, a low pass filter is demonstrated by cascading three proposed unit cells, which shows cutoff frequency of 1.53GHz and deep attenuation from 2.23GHz to 4.49GHz lower than -50dB.

• 대한의생명과학회지
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• 제18권1호
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• pp.71-75
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• 2012

The PLA2G4A catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid, which is metabolized into lipid-based cellular hormones that regulate inflammatory response. The circulating blood cell numbers can be influenced by stress, infection or inflammation. Quantitative blood cell count traits analysis for the 19 SNPs in the PLA2G4A gene in the Korean Association Resource (KARE) cohort (7551 subjects) was performed. The only one SNP (rs10752979) in the all blood cell count was satisfied with the Bonferroni corrected P-value (<0.00263). Furthermore, 6 of the 19 SNPs in the PLA2G4A gene showed a weak or moderate association with blood cell count (P-values: 0.0048~0.042), suggesting the clue of an association between the PLA2G4A gene and blood cell count, especially white blood cell count. This study may provide insight into the genetic basis of blood cell count related with reaction of infection.

• Journal of Microbiology
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• 제33권3호
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• pp.228-233
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• 1995

As S. epidermidis cell was fractionated into cell wall, cell membrane, and cytoplasm, the cell membrane proved to be the most efficient absorbent for lead ion. Utrasonication was effective, when the cells were treated during their exponential growth. The amount of the lead ion adsorbed in cell membrane decreased as hydrogen ion concentration of solution increased. Protein purified from the cell membrane showed higher adsorption capacity for the lead ion than peptidoglycan, teichoic acid from cell wall, or cell membrane lipid. Modification of carboxyl groups in the membrane protein with ethylenediamine and 1-ethyl-3-carbodiimide hydrochloride resulted in a considerable decrease of lead ion adsorption capability, suggesting that the main binding site for lead ion was the carboxyl groups of protein in cell membrane.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.2435-2440
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• 2003

Manipulation of the nano/micro scale object has been a key technology in biology as the sizes of DNA, chromosome, nucleus, cell and embryo are within such order. For instance, for embryo cell manipulation, the cell injection is performed manually. The operator often spends over a year to carry out a cell manipulation project. Since the typical success rate of such operation is extremely low, automation of such biological cell manipulation has been asked. As the operator spends most of his time in finding the position of cell in the Petri dish and in injecting bio-material to the cell from the best orientation. In this paper, we propose a new strategy and a vision system, by which one can find, recognize and track nucleus, polar body, and zona pellucida of the embryo cell for automatic biomanipulation. The deformable template matching algorithm has been used in recognizing the nucleus and polar body of each cell. Result suggests that it outperforms the conventional methods.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4007-4011
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• 2012

Purpose: Retinoblastoma-interacting zinc finger gene (RIZ1) is a tumor suppressor gene which is highly inactivated by promoter hypermethylation in patients with liver fluke-related cholangiocarcinoma (CCA). Epigenetic aberration of this gene might withdraw the ability to restrain tumor cell proliferation and migration. We aimed to define the role of RIZ1 on cell proliferation and migration in CCA cell line. Materials and methods: Small interference RNA (siRNA) was used to knock down the expression of RIZ1 in a CCA-derived cell line in which cell proliferation and cell migration were performed. Results: A predominant nuclear localization of RIZ1 was observed. Reduction of RIZ1 by siRNA augmented cell proliferation and migration. Conclusion: The result suggested that RIZ1 might play a role in regulating cell proliferation and migration in CCA. Reduction of RIZ1 expression may aggravate the progression of CCA.

• Preventive Nutrition and Food Science
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• 제18권2호
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• pp.92-97
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• 2013

The calcification of vascular smooth muscle cells (VSMCs) is considered one of the major contributors for vascular disease. Phosphate is known as the inducer for VSMC calcification. In this study, we assessed whether phosphate affected cell viability and fetuin-A, a calcification inhibitor protein, both which are related to VSMC calcification. Also, VSMC viability by zinc level was assessed. The results showed that phosphate increased Ca and P deposition in VSMCs (A7r5 cell line, rat aorta origin). This phosphate-induced Ca and P deposition was consistent with the decreased A7r5 cell viability (P<0.05), which implies phosphate-induced calcification in A7r5 cells might be due to the decreased VSMC cell viability. As phosphate increased, the protein expression of fetuin-A protein was up-regulated. A7r5 cell viability decreased as the addition of cellular zinc level was decreased (P<0.05). The results suggested that zinc deficiency causes the decreased cell viability and it would be the future study to clarify how zinc does act for VSMC cell viability. The results suggest that the decreased VSMC viability by high P or low Zn in VSMCs may be the risk factor for vascular disease.

• 미생물학회지
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• 제10권1호
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• pp.29-34
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• 1972

The present study is of ultra-fine structure of Cryptococcus neoformans by means of electron microscopy and reveals the following : 1) In constrast to the bacteria, the normal Cryptococcus neofrmans contains nuclear enveloped with nuclear menbrane, mitochondria, endoplasmic reticulum, distinct cell wall and cell membrane, vacuoles and storage granules as observed in the eucaryotic cells. 2) In apparent cell walls and cell membrane with the appearance of electron transparent area (ETA) and changes of cell morphology were observed in the ultra-violet ray irradiated cell. 2) In apparent cell walls and cell membrance with the appreance of electron transparent area (ETA) and changes of cell morphology were observed in the ultra-violet ray irradiated cell. 3) Morphology changes and cytoplasmic element abnormality was increased with irradiated time. 4) Increase of electron transparent area was thought to be associated with degradation of cell.

• International Journal of Oral Biology
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• 제37권3호
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• pp.115-120
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• 2012

Retinoic acid plays an important role in the regulation of cell growth and differentiation. In our present study, we evaluated the effects of all-trans retinoic acid (RA) on cell proliferation and on the cell cycle regulation of human gingival fibroblasts (HGFs). Cell proliferation was assessed using the MTT assay. Cell cycle analysis was performed by flow cytometry, and cell cycle regulatory proteins were determined by western blot. Cell proliferation was increased in the presence of a 0.1 nM to 1 ${\mu}M$ RA dose range, and maximal growth stimulation was observed in cells exposed to 1 nM of RA. Exposure of HGFs to 1 nM of RA resulted in an augmented cell cycle progression. To elucidate the molecular mechanisms underlying cell cycle regulation by RA, we measured the intracellular levels of major cell cycle regulatory proteins. The levels of cyclin E and cyclin-dependent kinase (CDK) 2 were found to be increased in HGFs following 1 nM of RA treatment. However, the levels of cyclin D, CDK 4, and CDK 6 were unchanged under these conditions. Also after exposure to 1 nM of RA, the protein levels of $p21^{WAF1/CIP1}$ and $p16^{INK4A}$ were decreased in HGFs compared with the control group, but the levels of p53 and pRb were similar between treated and untreated cells. These results suggest that RA increases cell proliferation and cell cycle progression in HGFs via increased cellular levels of cyclin E and CDK 2, and decreased cellular levels of $p21^{WAF1/CIP1}$ and $p16^{INK4A}$.