• Journal of the Korean Society for Precision Engineering
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• v.21 no.12
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• pp.174-181
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• 2004

Ultra precision positioning mechanism has widely been used on semiconductor manufacturing equipments, optical spectrum analyzer and cell manipulations. Ultra precision positioning mechanism is consisted of several actuators, sensors, guides and control systems. Its efficiency depends on each performance of components. The object of this study is to design, analysis and manufacture all of the inchworm linear motor system, which is one of the equipments embodied in ultra precision positioning mechanism. Inchworm linear motor system is consisted of a controller system and an inchworm linear motor, and its driving form is similar to a motion of spanworm. A design and manufacture of inchworm linear motor, which is consisted of three PZT actuators, a rod, two columns and a guide plate, are performed. Minimizing the von-Mises stress of the hinge using Taguchi method and simulation by FEM software optimizes the structural design in a column of flexure hinge. The designed columns and guide plates are manufactured by a W-EDM and NC-milling. A controller system, which is an apparatus to drive inchworm linear motor, can easily adjust driving conditions by varying resonance frequency and input-output voltage of actuators and amplifiers. The performance of manufactured inchworm linear motor system is verified and valuated. In the future, inchworm linear motor system will be used to make a more precision positioning by reinforcing a sensor and feedback system.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.1
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• pp.21-28
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• 2018

CRISPR/Cas9 gene editing system is an efficient method to mutation in a sequence specific manner. Here we report the direct transfection of the Cas9 nuclease and gene specific guide RNA can be used in BM-N cell line derived from Bombyx mori ovarian tissue to enfeeble function of endogenous gene in vitro. We have used gene editing system to negative regulation components of major signaling cascade, the Toll pathway, which controls B. mori resistance to microbe infections, such as fungi and gram positive bacteria. We demonstrate that the $I{\kappa}B-like$ protein Cactus may controls the activation of transcription factors such as Rel A and Rel B. The direct transfection of Cas9 nuclease and Cactus-specific guide-RNA complex may be used in BM-N cells to disrupt the function of endogenous genes in vitro. A mutation frequency of 30-40% was observed in the transfected cells, and various mutations caused the target region. Moreover, RT-PCR analysis revealed that Cactus gene was down regulated after these mutations. More importantly, mutation of BmCactus stimulated expression of lysozyme, moricin, and lebocin genes. These results suggest that the CRISPR/Cas9 systems are expected to efficiently induce site-specific mutations and it was possible to produce antimicrobial peptide through the gene editing.

• Journal of the Korea Society of Computer and Information
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• v.15 no.4
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• pp.75-82
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• 2010

At this time, our country's social welfare system for the handicapped is switching to increase independence, education is the most important. This study increases the quality of Visual handicapped people's life which have limit to learn and reduces their inconvenience which may occur during their independent school life through the development of the system which helps them safely to reach their destination by themselves using IT technologies in an educational institute which helps their ability to get information and be independent. This study supports Visual handicapped people to move safely by using cell phone which is necessary items for most modern people, RFID tags and white canes with antenna and reader which read the information installed in the raised blocks outside of school buildings and in the hall or aisle inside of buildings and inform the path by voice and vibration.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.45.1-45.1
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• 2016

Spatially rotating magnetic fields have been observed in the solar wind and in the Earth's magnetopause as well as in reversed field pinch (RFP) devices. Such field configurations have a similarity with extended current layers having a spatially varying plasma pressure instead of the spatially varying guide field. It is thus expected that magnetic reconnection may take place in a rotating magnetic field no less than in an extended current layer. We have investigated the spontaneous evolution of a collisionless plasma system embedding a rotating magnetic field with a two-and-a-half-dimensional electromagnetic particle-in-cell (PIC) simulation. In magnetohydrodynamics, magnetic flux can be decreased by diffusion in O-lines. In kinetic physics, however, an asymmetry of the velocity distribution function can generate new magnetic flux near O- and X-lines, hence a dynamo effect. We have found that a magnetic-flux-reducing diffusion phase and a magnetic-flux-increasing dynamo phase are alternating with a certain period. The temperature of the system also varies with the same period, showing a similarity to sawtooth oscillations in tokamaks. We have shown that a modified theory of sawtooth oscillations can explain the periodic behavior observed in the simulation. A strong guide field distorts the current layer as was observed in laboratory experiments. This distortion is smoothed out as magnetic islands fade away by the O-line diffusion, but is soon strengthened by the growth of magnetic islands. These processes are all repeating with a fixed period. Our results suggest that a rotating magnetic field configuration continuously undergoes deformation and relaxation in a short time-scale although it might look rather steady in a long-term view.

• Genomics & Informatics
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• v.19 no.4
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• pp.47.1-47.12
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• 2021

Kaposi's sarcoma-associated herpesvirus (KSHV) is one of the few human oncogenic viruses, which causes a variety of malignancies, including Kaposi's sarcoma, multicentric Castleman disease, and primary effusion lymphoma, particularly in human immunodeficiency virus patients. The currently available treatment options cannot always prevent the invasion and dissemination of this virus. In recent times, siRNA-based therapeutics are gaining prominence over conventional medications as siRNA can be designed to target almost any gene of interest. The ORF57 is a crucial regulatory protein for lytic gene expression of KSHV. Disruption of this gene translation will inevitably inhibit the replication of the virus in the host cell. Therefore, the ORF57 of KSHV could be a potential target for designing siRNA-based therapeutics. Considering both sequence preferences and target site accessibility, several online tools (i-SCORE Designer, Sfold web server) had been utilized to predict the siRNA guide strand against the ORF57. Subsequently, off-target filtration (BLAST), conservancy test (fuzznuc), and thermodynamics analysis (RNAcofold, RNAalifold, and RNA Structure web server) were also performed to select the most suitable siRNA sequences. Finally, two siRNAs were identified that passed all of the filtration phases and fulfilled the thermodynamic criteria. We hope that the siRNAs predicted in this study would be helpful for the development of new effective therapeutics against KSHV.

• Preventive Nutrition and Food Science
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• v.7 no.4
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• pp.454-460
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• 2002

While atherosclerosis is a major killer, there is now concern that mortality from the disease will increase due to the rising incidence of type II diabetes. Because diet can potentially influence both diseases, it is important to elucidate the role of diet in the progression of atherosclerosis. In addition, the mechanisms involved in dietary-related alterations of the disease need to be defined to guide public health recommendations to reduce athero-sclerosis incidence and limiting unwanted side effects. Since diet is thought to play a role in atherosclerosis even without added complications due to type II diabetes, reducing the incidence of that metabolic disease will not be enough. While evidence is increasing that high intake of carbohydrate can lead to type II diabetes and atherosclerosis, the preponderance of existing evidence indicates that intake of specific fats as a major dietary causal factor. It has recently been hypothesized that a dietary fat link to atherosclerosis may depend partly on the activity of a transcriptional regulator, the peroxisome proliferator activated receptors (PPAR). Thusfar, PPAR $\alpha$, $\beta$/$\delta$ and ${\gamma}$, have been shown to play a major role in metabolism, inflammation, and cancer. Furthermore, PPAR may regulate specific processes associated with atherosclerosis such as triglyceride and low density lipoprotein (LDL) metabolism; the reverse cholesterol transport pathway; lipid accumulation within plaques; the local inflammatory response and plaque stability. Synthetic ligands for PPAR have been developed; however, natural ligands include specific fatty acids and their metabolites. Though the role of PPAR in atherosclerosis has been reported with respect to synthetic ligands, additional studies need to be done with established and possible natural ligands. In this review, we will focus on the relation of dietary fat to PPAR alteration of atherosclerosis.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.8
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• pp.4621-4625
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• 2013

The purpose of this study was to construct a protein-protein interaction (PPI) network related to oral squamous cell carcinoma (OSCC). Each protein was ranked and those most associated with OSCC were mined within the network. First, OSCC-related genes were retrieved from the Online Mendelian Inheritance in Man (OMIM) database. Then they were mapped to their protein identifiers and a seed set of proteins was built. The seed proteins were expanded using the nearest neighbor expansion method to construct a PPI network through the Online Predicated Human Interaction Database (OPHID). The network was verified to be statistically significant, the score of each protein was evaluated by algorithm, then the OSCC-related proteins were ranked. 38 OSCC related seed proteins were expanded to 750 protein pairs. A protein-protein interaction nerwork was then constructed and the 30 top-ranked proteins listed. The four highest-scoring seed proteins were SMAD4, CTNNB1, HRAS, NOTCH1, and four non-seed proteins P53, EP300, SMAD3, SRC were mined using the nearest neighbor expansion method. The methods shown here may facilitate the discovery of important OSCC proteins and guide medical researchers in further pertinent studies.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.3
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• pp.189-196
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• 2018

Propeller performance measurement system for high altitude UAV was designed and applied to the wind tunnel test for 2 propeller models with a diameter around 1 m. Mechanical power of the propeller was directly measured by using the torque sensor installed on the rotating axis. The thrust of whole operation body including the propeller was measured by thrust road cell. The guide rail system was suggested to reduce the weight influence of operation body on the thrust road cell. The influence of each measured variables on the aerodynamic coefficients was studied with the repeatability and uncertainty analysis. This analysis result shows that the accuracies of the road cell and the wind velocity were major factors for the thrust coefficient. Propeller performance with typical RPM was measured with various wind speeds and the test results was summarized by performance coefficients for 5 different RPM.

• Journal of Sensor Science and Technology
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• v.18 no.6
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• pp.423-431
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• 2009

Standard uroflowmetry measures the urine weight using single load cell to evaluate the urinary flow rate. Impact noise should be introduced due to gravity when the urine stream falls down into the container upon the load cell. The present study placed three load cells on the three vertices of a regular triangle and the three signals were ensemble averaged to enhance the signal-to-noise ratio(SNR) regardless of how the urination was made. Simulated urination experiment was performed with three different urine collection methods. In all three methods, SNR of the averaged signal was much higher than each load cell signals. With no urine collection device, the present signal averaging technique resulted in SNR values higher by 10~15 dB than when dual funnels or upper funnel were used to guide the urine stream. Therefore, it was demonstrated that the three point measurement followed by with ensemble averaging could enable accurate uroflowmetric test without any specially made urine collection devices.

• Asian Oncology Nursing
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• v.10 no.2
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• pp.119-128
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• 2010

Purpose: This study aimed to evaluate the effectiveness of continuous nutritional education and oral mucositis management on the nutritive status of patients who received hematopoietic stem cell transplantation (HSCT). Methods: After randomly allotting 72 patients who received HSCT to either an experimental group or a control group, intensive and continuous care for preventing malnutrition was conducted in the experimental group while usual routine care was conducted in the control group. The changes of the body scale, blood chemistry profile, oral intake calories, nausea and vomitus, and oral stomatitis scores were measured at three points during their hospitalization using a oral assessment guide and nutrition analysis program: admission, HSCT, and discharge day. The differences between the scores of two groups were analyzed by repeated measures analysis of covariance. Results: The number of total lymphocytes was significantly improved in the experimental group after transplantation (p<.001). Nausea and vomiting score was significantly decreased in the experimental group during the conditioning regimen (p<.001). Conclusion: It was found that continuous nutritional education and oral mucositis control is an effective intervention by improving immune condition. Further investigations concerning direct examination of oral intake with controlling the effect of the chemotherapy are needed to ultimately discern the impact of varying oral nutrition patterns during HSCT.