• Genomics & Informatics
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• v.8 no.4
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• pp.185-193
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• 2010

Histone deacetylation and demethylation are epigenetic mechanisms implicated in cancer. Studies regarding the role of modulation of gene expression utilizing the histone deacetylase inhibitor scriptaid and the demethylating agent 5-azacytidine in HL-60 leukemia cells have been limited. We studied the possibility of recovering epigenetically silenced genes by scriptaid and 5-azacytidine in human leukemia cells by DNA microarray analysis. The first group was leukemia cells that were cultured with 5-azacytidine. The second group was cultured with scriptaid. The other group was cultured with both agents. Two hundred seventy newly developed genes were expressed after the combination of 5-azacytidine and scriptaid. Twenty-nine genes were unchanged after the combination treatment of 5-azacytidine and scriptaid. Among the 270 genes, 13 genes were differed significantly from the control. HPGD, CPA3, CEACAM6, LOC653907, ETS1, RAB37, PMP22, FST, FOXC1, and CCL2 were up-regulated, and IGLL3, IGLL1, and ASS1 were down-regulated. Eleven genes associated with oncogenesis were found among the differentially expressed genes: ETS1, ASCL2, BTG2, BTG1, SLAMF6, CDKN2D, RRAS, RET, GIPC1, MAGEB, and RGL4. We report the results of our leukemia cell microarray profiles after epigenetic combination therapy with the hope that they are the starting point of selectively targeted epigenetic therapy.

• Korean Journal of Food Science and Technology
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• v.25 no.4
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• pp.391-394
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• 1993

For the automation of Tackjoo fermentation, a sensor measurable gas production during brewing and a controller were built. The performance tests were carried out at 10 litter Tackjoo fermentor, The sensor was consisted of a transparent acryl cell for bubble formation and photo-interrupter for the detection of bubbles of 0.018ml size. The fermentation controller was fabricated with a single chip microcomputer (MC68705R3) and provided with both the monitoring module of temperature measurement and the valve controling device for the cooling water circulation in coil type heat exchanger. The operation programs were developed and systemized in ROM. With this computer system, the gas production amount and rate were acquired during the Tackjoo fermentation. The fermentation curve based on the gas production rate showed a good agreement with that of alcohol concentration. The maximum rate of gas production was found after 24 hr at $30^{\circ}C$. The correlation equation between the gas production and alcohol concentration was established and used as the control algorithm of the fermentation.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.11
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• pp.124-129
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• 2013

This paper proposes a high-performance vector inner product calculation circuit for real-time object recognition based on SVM algorithm. SVM algorithm shows a higher detection rate than other object recognition algorithms. However, it requires a huge amount of computational efforts. Since vector inner product calculation is one of the major operations of SVM algorithm, it is important to implement a high-performance vector inner product calculation circuit for real-time object recognition capability. The proposed circuit adopts the pipeline architecture with six stages to increase the operating speed and makes it possible to recognize objects in real time based on SVM. The proposed circuit was described in Verilog HDL at RTL. For silicon verification, an MPW chip was fabricated using TSMC 180nm standard cell library. The operation of the implemented MPW chip was verified on the test board with test application software developed for the chip verification.

• Proceedings of the IEEK Conference
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• 2002.07a
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• pp.670-673
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• 2002

This paper proposes a digital algorithm for gray-scale/color image segmentation of real-time video signals and a cell-network-based implementation architecture in state-of-the-art CMOS technology. Through extrapolation of design and simulation results we predict that about 300$\times$300 pixels can be integrated on a chip at 100nm CMOS technology, realizing very high-speed segmentation at about 1600sec per color image. Consequently real-time color-video segmentation will become possible in near future.

• Proceedings of the KIEE Conference
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• 1992.07b
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• pp.1041-1044
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• 1992

A solar cell should be operated at the maximum output point on the I-V characteristic curve with constant current and constant voltage in order that the solar energy be fully utilized. According to, in this paper, we describes a controller which can track the maximum power point of a solar arry using current and voltage ripple variation of step up chopper system. The control circuit is desinged such that actual current and voltage are sensed directly from the solar cell array. These two signal are then holded sampling and multiplies by a single chip multiplier.

• JSTS:Journal of Semiconductor Technology and Science
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• v.16 no.6
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• pp.781-792
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• 2016

This paper presents a new approach to enhance the data retention of logic-compatible embedded DRAMs. The memory bit-cell in this work consists of two logic transistors implemented in generic triple-well CMOS process. The key idea is to use the parasitic junction capacitance built between the common cell-body and the data storage node. For each write access, a voltage transition on the cell-body couples up the data storage levels. This technique enhances the data retention and the read performance without using additional cell devices. The technique also provides much strong immunity from the write disturbance in the nature. Measurement results from a 64-kbit eDRAM test chip implemented in a 130 nm logic CMOS technology demonstrate the effectiveness of the proposed circuit technique. The refresh period for 99.9% bit yield measures $600{\mu}s$ at 1.1 V and $85^{\circ}C$, enhancing by % over the conventional design approach.

• Journal of Korean Institute of Industrial Engineers
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• v.40 no.3
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• pp.257-266
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• 2014

The semiconductor manufacturing industry is managed by a number of parameters from the FAB which is the initial step of production to package test which is the final step of production. Various methods for prediction for the quality and yield are required to reduce the production costs caused by a complicated manufacturing process. In order to increase the accuracy of quality prediction, we have to extract the significant features from the large amount of data. In this study, we propose the method for extracting feature from the cell level data of probe test process using OPTICS which is one of the density-based clustering to improve the prediction accuracy of the quality of the assembled chips that will be placed in a package test. Two features extracted by using OPTICS are used as input variables of quality prediction model because of having position information of the cell defect. The package test progress for chips classified to the correct quality grade by performing the improved prediction method is expected to bring the effect of reducing production costs.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.4
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• pp.1041-1054
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• 2004

Electroacupuncture (EA) has been reported to increase pain threshold, and to enhance the NK cell activity by up-regulation of IFN-γ and endogenous β-endolphin. For the purpose of understanding the molecular mechanism of EA stimulation, we analyzed the gene expression profile of rat hypothalamus, treated on Zusanli (ST36) with EA, in comparison with control group by oligonucleotide chip microarray (Affymetrix GeneChip Rat Neurobiology U34 Array) and real-time RT-PCR. Sprague-Dawley (S-D) male rats were stimulated at the Zusanli (ST36) acupoint in restriction holder. Simultaneously the control group was given only holder stress without EA stimulation. In order to prove the appropriateness of EA treatment, we measured spleen NK cell activity with standard 51Cr release assay. NK cell activity of EA group was significantly increased comparing to control group. The microarray and PCR results show that EA treatment up-regulates expression of genes associated with 1) nerve growth such as NGF induced factor A and VGF, 2) signal transduction such as 5HT3 receptor subunit, AMPA receptor binding protein and Na-dependent neurotransmitter transporter, and 3) anti-oxidation such as superoxide dismutase and glutathione S-transferase. In addition, the activity of the anti-oxidative enzyme, SOD of hypothalamus, liver and RBC was enhanced compared to that of control. The list of differentially expressed genes may implicate further insight on the mechanism of acupuncture effects.

• Analytical Science and Technology
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• v.22 no.6
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• pp.498-503
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• 2009

Lung cancer is the main cancer on the world today, due to the high case fatality. Lung cancer can devide into two major types, such as small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Mutations in the epidermal growth factor receptor (EGFR) have been described in patients with advanced NSCLC. Mutations in the EGFR are associated with clinical and radiographic responses to EGFR tyrosine kinase inhibitors gefitinib and erlotinib. Thus, the detection of EGFR mutation can offer an effective information in clinical decision-making. In this study, We developed very simple, cheep and rapid mutation detection system by chip-based isothermal amplification method. The method described here has shown the advantages of rapid amplification, high sensitivity, and specificity. Also, it will be useful for rapid and reliable clinical diagnosis of EGFR mutation.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.3
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• pp.61-71
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• 2014

As the occupancy rate of the flash memory increases in the storage media market for the embedded system and the semi-conductor industry grows, the demand and supply of flash memory is increasing by a big margin. They are especially used in large quantity in the smart phones, tablets, PC, SSD and Soc(System on Chip) etc. The flash memory is divided into the NOR type and NAND type according to the cell arrangement structure and the NAND type is divided into the SLC(Single Level Cell) and MLC(Multi Level Cell) according to the number of bits that can be stored in each cell. Many tests have been performed on NOR type such as BIST(Bulit-In Self Test) and BIRA(Bulit-In Redundancy Analysis) etc, but there is little study on the NAND type. For the case of the existing BIST, the test can be proceeded using external equipments like ATE of high price. However, this paper is an attempt for the improvement of credibility and harvest rate of the system by proposing the BIST for the MLC NAND type flash memory of Finite State Machine structure on which the pattern test can be performed without external equipment since the necessary patterns are embedded in the interior and which uses the MLC NAND March(x) algorithm and pattern which had been proposed for the MLC NAND type flash memory.