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http://dx.doi.org/10.5806/AST.2009.22.6.498

Chip-based isothermal amplification method for EGFR gene mutations in lung cancer  

Ahn, Young-Chang (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Park, Su-Min (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Seo, Jae-Won (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Yoon, Il-Kyu (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Jung, Duck-Hyun (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Lee, Eun-Young (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Nam, Youn-Hyoung (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Jang, Won-Cheoul (Department of Chemistry, School of Advanced Science and Basic Science Research Institute, Dankook University)
Seung, Kwon Pil (Department of Laboratory Medicine, Dankook University Hospital)
Kim, Jong-Wan (Department of Laboratory Medicine, Dankook University College of Medicine)
Publication Information
Analytical Science and Technology / v.22, no.6, 2009 , pp. 498-503 More about this Journal
Abstract
Lung cancer is the main cancer on the world today, due to the high case fatality. Lung cancer can devide into two major types, such as small-cell lung cancer (SCLC) and non-small-cell lung cancer (NSCLC). Mutations in the epidermal growth factor receptor (EGFR) have been described in patients with advanced NSCLC. Mutations in the EGFR are associated with clinical and radiographic responses to EGFR tyrosine kinase inhibitors gefitinib and erlotinib. Thus, the detection of EGFR mutation can offer an effective information in clinical decision-making. In this study, We developed very simple, cheep and rapid mutation detection system by chip-based isothermal amplification method. The method described here has shown the advantages of rapid amplification, high sensitivity, and specificity. Also, it will be useful for rapid and reliable clinical diagnosis of EGFR mutation.
Keywords
Chip; EGFR (Epidermal Growth Factor Receptor); Isothermal Amplification; Non-Small-Cell Lung Cancer (NSCLC);
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