• 대한두개안면성형외과학회지
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• 제22권4호
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• pp.199-203
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• 2021

Primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder is a rare disease characterized by a single mass on the face or upper part of the trunk. It usually presents an asymptomatic and favorable progression, and its histopathologic findings include small and medium-sized lymphoid cells. The authors report a case of primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder on the forehead. A 51-year-old man presented with a protruding mass on his forehead that the patient had noted 1 month previously. Surgical excision and a permanent biopsy were performed under local anesthesia. Based on the biopsy results, the mass was diagnosed as a primary cutaneous CD4+ small/medium T-cell lymphoproliferative disorder. There was no evidence of recurrence at a 15-month follow-up visit.

• 대한두개안면성형외과학회지
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• 제14권2호
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• pp.129-132
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• 2013

Cutaneous squamous cell carcinoma is the second-most common skin cancer and represents 20% of all skin cancers. Cutaneous squamous cell carcinoma often spreads to the parotid gland through lymph nodes, but, direct invasion of an adjacent organ may also occur. We present the case of 78-year-old man with ulcerated mass on the right infra-auricular area. The histopathologic finding was squamous cell carcinoma. There was no evidence of distant metastasis, but the mass was found to invade the superficial lobe of the right parotid gland. The mass was widely excised and superficial parotidectomy was performed while preserving the facial nerve. The defect was covered by primary closure. Postoperative radiotherapy was performed. At 20 months after surgery, our patient had no facial palsy, local recurrence, or metastasis. Cutaneous squamous cell carcinoma involving the parotid gland is an aggressive, rapidly advancing lesion, which if not recognized and treated early will result in high morbidity and mortality. Squamous cell carcinoma of the parotid gland has shown that patients who receive adjuvant radiotherapy have a lower recurrence rate and a higher survival rate than patients treated with surgery alone. The role of elective neck dissection remains controversial.

• Archives of Plastic Surgery
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• 제33권6호
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• pp.757-760
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• 2006

Purpose: Anaplastic large cell lymphoma, has the following three characteristics of a malignant lymphoma; 1) An irregular large nucleus, called pathologic atypical cells, 2) Eosinophilic cytoplasm, 3) Immunologically positive for Ki-1. Anaplastic large cell lymphoma occurs mostly in the lymph nodes, but about 40% has been observed to occur in other tissues. Skin is the one of the main sources of origin and it is called 'primary cutaneous anaplastic large cell lymphoma'. Methods: A 69-year-old male patient with an erythematous nodule, sized $1.5{\times}1.7cm$ on his right hand dorsum was excised under local anesthesia and on biopsy was diagnosed as 'Dermatofibrosarcoma Protuberans'. Three months after the local excision and biopsy, same natured mass reoccurred in the same region, and then spontaneous regressed after three weeks. However, metastatic large mass of $4.0{\times}5.0cm$, of same nature was observed on the elbow. The large mass was operated with wide excision and biopsy. Results: On final diagnosis, with an immunofluorescent stain with CD30(Ki-1), 'Primary cutaneous large cell lymphoma' was made. After follow up for three years, we did not observed recurrence and metastasis. Conclusion: We have reported that we have diagnosed primary cutaneous large cell lymphoma and treated without recurrence and metastasis.

• Mass Spectrometry Letters
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• 제11권2호
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• pp.36-40
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• 2020

Untargeted metabolomics is a useful tool for drug development focusing on novel chemotherapeutic and chemopreventative agents against cancer cells. In recent years, quadrupole time of flight liquid chromatography-mass spectrometry (Q-TOF LC/MS)-based untargeted metabolomic approaches have gained importance to evaluate the effect of these agents at the molecular level. The researchers working on cell culture studies still do not apply standardized methodologies on sample preparation for untargeted metabolomics approaches. In this study, the rough and wet lysis techniques performed on MCF-7 breast cancer cells were compared with each other via the Q-TOF LC/MS-based metabolomic approach. The C18 and hydrophilic interaction liquid chromatography (HILIC) columns were used for the separation of the metabolites in MCF-7 cell lysates. 505 peaks were detected through the HILIC column and 551 peaks were found through the C18 column for the wet lysis technique. This situation supported by the base peak chromatograms showed that the wet lysis technique allowed us to extract higher number of non-polar metabolites. Almost equal number of metabolites was found for the C18 and HILIC columns (697 peaks for the HILIC column and 695 peaks for the C18 column) when the rough lysis technique was used. However, the intensities of polar metabolites were higher for the rough lysis technique on base peak chromatograms for both the HILIC and C18 columns. Although cell lysis technique, which is the first step in the sample preparation for cell culture studies, does not cause dramatic differences in the number of the detected metabolite peaks, it affects the polar and non-polar metabolite ratio significantly. Therefore, it must be considered carefully especially for in vitro drug development studies.

• 한국미생물·생명공학회지
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• 제16권4호
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• pp.282-286
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• 1988

Chinese Hamster Ovary 세포의 대량배양을 위한 동력학적 변수들을 산소 소비속도의 on-line 측정으로 구하였다. 세포성장과 산소 소비속도의 상관관계 계수가 0.83으로 밀접한 상호 직선관계가 있음을 입증하며, 이에 근거를 둔 model에 의해 간접 계산된 세포수가 실제 측정된 세포수와 상당히 일치하였다. 따라서 세포끼리 서로 뭉쳐 직접 세포수 측정이 부정확한 경우 산소 소비속도의 측정에 의해 간접적으로 세포수를 예측할 수 있음을 입증하였다. 산소 세포수율, Yx/o와 mass transfer coefficient, $K_{\ell}$a가 각각 1.26$\times$$10^4$cells/mmole $O_2$ consumed와 1.011/h로 측정되었으며, 평균 세포 성장속도는 2.891/day로 계산되었다. 이는 매일 2 gram의 tPA를 연속 생산할 수 있는 조건이었다.

• 한국미생물·생명공학회지
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• 제5권1호
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• pp.18-23
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• 1977

섬유소자화세균 Cellulomonas flavigena KIST 321로 섬유소 단세포 단백을 생산하기 위하여 우리주변에서 제기되고 있는 각종 섬유소폐기물을 수집하여 기질로서의 사용 가능성을 검토하였는 바 그 결과를 요약하면 다음과 같다. 1. 당유를 유일한 탄소원으로 하였을 때 cellulose와 rylose에서 균체량이 가장 많았다. 2. 폐지유와 폐의유를 기질로 하였을 때 알카리 전처리를 함으로써 알카리 전처리하지 않았을 때보다 균체량이 많았으며, 폐신문지와 같은 인쇄물에서는 균체량이 적었다. 3. 짚류(straws)를 기질로 하였을 때는 대체로 균체수율이 높았으며, 볏짚, 채종대, 피를 기질로 하였을 때 균체량이 많았다. 4. 목재유의 톱밥에서는 균체량이 적었으나 잎류(leaves)에서는 비교적 많았다. 5. 기질의 종류에 따라 전처리시 NaOH농도를 다르게 하여 균체생산량을 조사하였다. NaOH 농도는 기질의 종류에 따라 최적농도가 달랐으나 짚류(Straws)에서는 0.8∼1.0％ 일때가 좋았고, 잎류(leaves)에서는 0.4∼0.6％일때가 좋았다. 6. 각종기질을 알카리 처리후 세척하여 기질로 사용하였을 때 볏짚에서는 균체량이 감소하였으나 소나무 톱밥, 피나무 톱밥, 소나무잎, 폐신문지에서는 증가하였다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2005년도 창립60주년 기념 추계 학술대회
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• pp.96.1-96.1
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• 2005

• 대한기관식도과학회지
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• 제6권2호
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• pp.181-184
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• 2000

Catsleman's disease is a type of angiofolliculr lymph node hyperplasia and its etiology is not known yet. It usually presents with the mediastinal mass but rarely with the parotid mass. There are only five cases previously reported in the literatures. It has two pathologic types, which are hyaline vascular type and plasma cell type. Plasma cell type is frequently associated with systemic menifestations. Diagnosis is based on the histopatholgic findings. Treatment is surgical excision. A case of Catsleman's disease involving the parotid lymph node is presented and literatures are reviewed.

• 한국임상수의학회지
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• 제14권2호
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• pp.365-369
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• 1997

A ten-year-old holstein cow was presented because of prolapse of the third eyelid and apparent hyperplasia of the right lower eyelid. Historical findings included increased appetite as well as polyuria and polydipsia for about two weeks. The most remarkable fadings on physical examination were a large periocular proliferative tissue and bleeding. Surgical incision was used both as a biopsy and therapeutic tool in holstein cow with mass. Histopathological examination of the mass revealed squamous cell carcinoma. Blood and milk tests of patient with squamous cell carcinomatosis were peformed, but normal values.

• Journal of Electrochemical Science and Technology
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• 제10권4호
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• pp.381-386
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• 2019

Zn-air battery uses oxygen from the air, and hence, air holes in it are kept open for cell operation. Therefore, loss of water by evaporation through the holes is inevitable. When the water is depleted, the battery ceases to operate. There are two water consumption routes in Zn-air batteries, namely, active path (electrolysis) and passive path (evaporation and corrosion). Water loss by the active path (electrolysis) is much faster than that by the passive path during the early stage of the cycles. The mass change by the active path slows after 10 h. In contrast, the passive path is largely constant, becoming the main mass loss path after 10 h. The active path contributes to two-thirds of the electrolyte consumption in 24 h of cell operation in 4.0 M KOH. Although water is an important component for the cell, water vapor does not influence the cell operation unless the water is nearly depleted. However, high oxygen concentration favors the discharge reaction at the cathode.