• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.77-77
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• 2003

Coculture of HSC with bone marrow-derived mesenchymal stem cells (BM-MSCs) is one of used methods to increase cell numbers before transplant to the patients. However, because of difficulties to purify HSCs after coculture with BM-MSCs, it needs to develop a method to overcome the problem. In the present study, we have examined whether a culture insert placed over a feeder layer might support the expansion of HSCs within the insert. $CD34^+/ $ cells isolated from the umbilical cord blood by using midiMACS were divided into three groups. A group of 1 $\times$ $10^5$ cells were grown on a culture insert without feeder layer (Direct). The same number of HSCs was directly cocultured with BM-MSCs (Contact). The third group was placed onto an insert below which BM-MSCs were grown (Insert). To distinguish feeder cells from HSCs, BM-MSCs was pre-labeled fluorescently with PKH26 and 1 $\times$ $10^5$ cells were seeded in the culture dishes. After culture for 13 days, the expansion factor (x) of HSCs that were grown without feeder layer (Direct) was $26.6 \pm 8.4.$ In contrast, the number of HSCs directly cocultured with feeder layer was 59.6 $\pm$ 0.5 and that of HSCs cultured onto an insert was $46.9 \pm 8.4.$ The percentage of BM-MSCs cells remained being fluorescent was $97.9 \pm 0.3%$ after culture. Immune-phenotypically large proportion of cultured cells were founded to be differentiated into myeloid/monocyte progenitor cells. The ability of BM-MSCs, fetal lung, cartilage and brain tissue cells to support ex vivo expansion of HSCs was also examined using the insert. After 11 days of coculture with each of these cells, the expansion factor of HSCs was 15.0, 39.0, 32.0 and 24.0, respectively. Based upon these observations, it is concluded that the coculture method using insert is very effective to support ex vivo expansion of HSCs and to eliminate the contamination of other cells used to coculture wth HSCs.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.265-268
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• 2007

플렉서블 태양전지용 연성기판재에는 플라스틱재와 금속재가 있다. 기존의 연성기판인 플라스틱의 경우 열과, 내구성, 화학약품에 약하다는 단점이 있으며, 금속기판은 높은 생산원가, 박판화의 어려움 등의 문제를 안고 있다. 상업적으로 응용되거나 연구에 활용되는 플렉서블 기판재의 단점을 보완할 수 있는 가능성을 밝혀보기 위해 전주성형법으로 합금 금속 포일을 제조하여 상용 금속 기판재의 열팽창 거동과 비교해 보았다. 본 연구에서는 플렉서블 태양전지용으로 적용되거나 연구되고 있는 금속 기판 재료인 두께 50 ${\mu}m$인 Ti, Mo, Al 포일을 선택하여 열팽창거동을 조사하였고 이를 전주성형법으로 제조한 두께 10 ${\mu}m$인 Fe-40Ni, Fe-45Ni, Fe-52Ni 합금포일의 열팽창 거동과 비교 분석하였다. 금속 및 합금 포일의 열팽창 거동은 TMA 장비를 사용하여 조사하였다.

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 1993.05a
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• pp.53-59
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• 1993

The interests on GaAs solar cell grown on Ge substrates as an alternative of GaAs substrate arises from its very close lattice parameters, very small difference in thermal expansion coefficients, and much higher fracture toughness between GaAs and Ge. In addition, for many space power application, it would be a most attractive solar cell with high radiation resistance of GaAs and high reliability for the reverse current damage of Ge, and expecting the theoretical efficiency limit of the tandem GaAs/Ge solar cell is 34% under 1 Sun, AM 0, and 28$^{\circ}C$ condition. In this report, we have reviewed the performance and the manufacturing technics of GaAs/Ge solar cell, and current status of research in GaAs/Ge solar cell.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.1
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• pp.26-31
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• 2023

Chicken embryonic stem (ES) cells have great potential and provide a powerful tool to investigate embryonic development and to manipulate genetic modification in a genome. However, very limited studies are available on the functional characterization and robust expansion of chicken ES cells compared to other species. Here, we have developed a method to generate chicken embryonic stem cell-like cells under pluripotent culture conditions. The chicken embryonic stem cell-like cells were cultivated long-term over several passages of culture without loss of pluripotency in vitro and had the specific expression of key stem cell markers. Furthermore, they showed severe changes in morphology and a significant reduction in pluripotent genes after siRNA-mediated NANOG knockdown. Collectively, these results demonstrate the efficient generation of chicken embryonic stem cell-like cells from EGK stage X blastoderm-derived singularized cells and will facilitate their potential use for various purposes, such as biobanking genetic materials and understanding stemness in the fields of animal biotechnology.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.28-28
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• 2001

The objective of this study was to the effect on subsequent development of EGF present in defined medium during bovine 1)oocyte maturation or 2)embryo culture. The presence of EGF during IVM, irrespective of concentration(1, 10, 100ng/$m\ell$), stimulated cumulus expansion and significantly increased the proportion of oocytes attaining metaphaseII, the rate of cleavage, and develop to blastocyst. 1. In the group of EGF-added medium(1, 10, 100ng/$m\ell$), nuclear maturation rate for in vitro maturation was 91% to 92% but was not significantly higher than control group(87%). 2. For in vitro maturation, in the group of EGF-added medium(1, 10, 100ng/$m\ell$)the rate of cumulus cell expansion degree 2 ranged from 81% to 87%, which was significantly higher than the control group(medium with EGF not added). The rate of in vitro fertilization, developing to 2-to 4- cell stage, was 76% to 80%, which was also significantly higher(p<0.05)than control group(62%). 3. For in vitro maturation, in the group of EGF added in medium(1, 10, 100ng/$m\ell$)the development rate to blastocyst was 24.3% to 27%, which was significantly higher than control group(13.7%). The total cleavage rate in the group of EGF-added medium was 77% to 82%, which was higher than control group. 4. The development rate to blastocyst for 6 days of cultivation and the hatching blastocyst were 30.6% and 59.1%, respectively, in the group of 100ng/$m\ell$ of EGF, which were significantly higher(p<0.05)than control group(14.0% and 24%, respectively), The numbers of cells in blastocyst were 140.2 and 148, respectively, in 10ng/$m\ell$ and 100ng/$m\ell$ of EGF-added medium, which were higher than 108.5 in control group. 5. The development rate of in vitro fertilized embryos to blastocyst in 10ng/$m\ell$ of EGF-added medium co-cultured with somatic cell was 28%, which was significantly higher(p<0.05)than control group(11.8%). The numbers of cells in blastocyst were 141.6 for EGF-added medium and 145 for EGF+co-culture group, which were higher than control(101.6)and medium co-cultured with somatic cells(110.6). These results showed that in vitro maturation and fertilization, EGF was found a significant effect of increase of development rate to blastocyst and cell number.

• Applied Chemistry for Engineering
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• v.9 no.7
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• pp.1090-1097
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• 1998

Lidocaine compounds have widely been used as local anesthetics. Regarding the molecular mechanism for anesthesia by lidocaine, it is proposed that lidocaine molecules penetrate to the hydrophobic region of cell membrane and expand the membrane volume, producing a change in protein conformation that blocks sodium permeability or lidocaine molecules directly adsorb into lidocaine receptor in the protein channel without expanding the cell membrane. But these proposals have never been proven experimentally. In this study, the expansion of cell membrane by lidocaine compounds was investigated by employing lipid monolayer at the air/water interface as the mimetic system of cell membrane. It was found that oil-soluble lidocaine contracted the area/molecule of lipid in the monolayer of phosphatidyl choline, sphingomyelin, DS-PL95E and lipoid, but expanded the monolayer of phosphatidyl ethanolamine only in a certain range of mixing ratios. On the contrary, water-soluble lidocaine-HCl salt expanded the monolayers of all lipids used in this study.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.9
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• pp.823-828
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• 2013

To reduce the cost per watt of photovoltaic power, it is important to reduce the cell thickness of crystalline silicon solar cells. As the thickness of the silicon layer is reduced, two distinctive thermal expansion rates between the silicon and the aluminum layer induce bowing in a solar cell. With a thinner silicon layer, the bowing distance grows exponentially. Excessive bowing could damage the silicon wafer. In this study, we tried to measure an irregularly curved silicon solar cell more accurately using a 3D image scanner. For the detailed analysis of the three-dimensional bowing shape, a least square fit was applied to the point data from the scanned image. It has been found that the bowing distance and shape distortion increase with a decrease in the thickness of the silicon layer. An Ag strip on top of the silicon layer can reduce the bowing distance.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.7
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• pp.1424-1432
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• 2015

As the wireless communication technologies are being studied for application to maritime communication networks in a fusion of marine industries and IT technology, interference coordination techniques have been studied in the maritime heterogeneous networks. In this paper, we develop a simulator for measuring, verifying and evaluating performance of a maritime heterogeneous network. Unlike other previous simulators, the developed simulator applies enhanced inter-cell interference coordination (eICIC) that are being introduced in the 3GPP Release 10 for mitigating the cross-tier interference between ships. Furthermore, we investigate the effects of almost blank subframes (ABS) and cell range expansion (CRE) on the throughput of small cells in maritime heterogeneous networks by using the developed simulator.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04a
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• pp.887-889
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• 2003

사다리꼴-분할 방식은 로봇 경로 계획 알고리즘 중 완전 셀-분할 방식중의 하나로서 장애물과 떨어진 경로를 제공하므로 안정성을 제공하는 방식이다. 사다리꼴-분할 방식은 다각형 환경으로 이루어진 형상공간에서 정의되며 자유공간을 볼록 다각형으로 이루어진 셀(cell)로 나누어 로봇 운동을 계획하는데, 원과 같은 비다각형 장애물이 존재하는 경우에 대해서는 이 성질을 만족하는 분할 방법이 알려져 있지 않다. 본 논문에서는 기존의 다각형 환경에서 정의된 사다리꼴-분할 방식을 분할의 완전성을 잃지 않고 원의 호를 포함하는 환경으로 확장하는 알고리즘을 소개하고 구현한다.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.4 s.97
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• pp.85-94
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• 1999

Reconfiguration, expansion, or new establishment of FMS requires the availability of a shop floor control (SFC) system relevant to the FMS since it is closely related with the hardware component of FMS. Due to the expensive cost of its development, significant research efforts have been made to develop an SFC system that is reusable. This paper presents Abstract Machine Tool (AMT) approach applied to develop an SFC sytem that is reusable without additional programming. The AMT model enables us to design the SFC system independently of the hardware-dependent attributes of euqipment; an AMT models a workstation by abstraction and presents an equipment-independent interface to machining cell controller. Specifically, we describe how we formalize the interfaces among equipment in order to build an AMT and how we design the machining cell control software based on AMT models. We also present MACHINIST the machining cell control system for IAE-FMS plant as an implementation example.