한국발생생물학회:학술대회논문집 (Proceedings of the Korean Society of Developmental Biology Conference)
- 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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- Pages.77-77
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- 2003
Ex Vivo Expansion of Hematopoietic Stem/Progenitor Cells by Coculture using Insert
- Kim, Kyung-Suk (Medical Research Center, Major Woman′s Care Center) ;
- Kim, Haekwon (Department of Biotechnology, Seoul Women′s University) ;
- Do, Byung-Rok (Biotechnology Research Institute, KTBiosys) ;
- Park, Seah (Department of Biotechnology, Seoul Women′s University) ;
- Kwon, Hyuck-Chan (Medical Research Center, Major Woman′s Care Center) ;
- Kim, Hyun-Ok (Department of Laboratory Medicine, Yonsei University) ;
- Im, Jung-Ae (Medical Research Center, Major Woman′s Care Center)
- 발행 : 2003.10.01
초록
Coculture of HSC with bone marrow-derived mesenchymal stem cells (BM-MSCs) is one of used methods to increase cell numbers before transplant to the patients. However, because of difficulties to purify HSCs after coculture with BM-MSCs, it needs to develop a method to overcome the problem. In the present study, we have examined whether a culture insert placed over a feeder layer might support the expansion of HSCs within the insert.