• 한국정보통신학회논문지
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• 제19권6호
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• pp.1307-1312
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• 2015

스마트폰의 대중화에 따라 무선 데이터 트래픽이 기하급수적으로 증가하고 있으며, 이러한 데이터 트래픽을 원활히 수용하기 위하여 차세대 이동통신 네트워크에 대한 연구가 활발히 진행 중이다. 특히, 매크로 셀과 소형 셀을 활용하여 공간 재활용성을 높임으로써 네트워크 용량을 획기적으로 개선할 수 있는 이종 이동 통신망이 많은 관심을 끌고 있다. 이종 이동 통신망에서는 매크로 기지국과 소형 기지국 간의 송신전력의 차이로 인하여 부하 불균형과 간섭등의 문제가 발생하며, 이를 해결하기 위하여 cell range expansion (CRE) 기술을 활용한다. 본 논문에서는, 초밀집 이종 이동 통신망 에서 CRE bias (CREB)를 적응적으로 적용하는 새로운 셀 선택 방식을 제안하고 시스템 레벨 시뮬레이션을 통하여 셀 평균 전송률을 분석하고, 기존의 셀 선택 방식과 비교 한다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 춘계학술대회
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• pp.63-66
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• 2015

스마트폰의 대중화에 따라 무선 데이터 트래픽이 기하급수적으로 증가하고 있으며, 이러한 데이터 트래픽을 원활히 수용하기 위하여 차세대 이동통신 네트워크에 대한 연구가 활발히 진행 중이다. 특히, 매크로 셀과 소형 셀을 활용하여 공간 재활용성을 높임으로써 네트워크 용량을 획기적으로 개선할 수 있는 이종 이동 통신망이 많은 관심을 끌고 있다. 이종 이동 통신망에서는 매크로 기지국과 소형 기지국 간의 송신전력의 차이로 인하여 부하 불균형과 간섭 등의 문제가 발생하며, 이를 해결하기 위하여 cell range expansion (CRE) 기술을 활용한다. 본 논문에서는, 초밀집 이종 이동 통신망에서 CRE bias를 적응적으로 적용하는 새로운 셀 선택 방식을 제안하고 시스템 레벨 시뮬레이션을 통하여 셀 평균 전송률을 분석하고, 기존의 셀 선택 방식과 비교 한다.

• Reproductive and Developmental Biology
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• 제30권4호
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• pp.287-291
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• 2006

We have developed a new passaging technique for the expansion of human embryonic stem cells (hESCs) that involves simply pipetting portions of hESCs acquired from colonies, reducing the laborious and time-consuming steps in the expansion of hESCs. Compared to general mechanical methods of passaging, our pipetting method allowed hESCs colonies to be broken into small fragments, which showed significantly higher attachment rates onto feeder cell layers. This technique produced three times the number of hESCs colonies than conventional mechanical methods. In addition, this pipetting method allowed us to distinguish differentiated hESCs from undifferentiated hESCs during hESCs colony pipetting. The hESCs cultured by pipetting method displayed normal human chromosomes for over 60 passages. According to RT-PCR and immunohistochemical analysis, the hESCs successfully maintained their undifferentiated state and pluripotency which was also confirmed by teratoma formation in viva Therefore, the pipetting method described in this study is a useful tool to efficiently and quickly expand hESCs on a large scale without enzyme treatment.

• Interaction and multiscale mechanics
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• 제3권3호
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• pp.213-234
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• 2010

A multiscale method is presented for analysis of thin slab structures in which the microstructures can not be reduced to two-dimensional plane stress models and thus three dimensional treatment of microstructures is necessary. This method is based on the classical asymptotic expansion multiscale approach but with consideration of the special geometric characteristics of the slab structures. This is achieved via a special form of multiscale asymptotic expansion of displacement field. The expanded three dimensional displacement field only exhibits in-plane periodicity and the thickness dimension is in the global scale. Consequently by employing the multiscale asymptotic expansion approach the global macroscopic structural problem and the local microscopic unit cell problem are rationally set up. It is noted that the unit cell is subjected to the in-plane periodic boundary conditions as well as the traction free conditions on the out of plane surfaces of the unit cell. The variational formulation and finite element implementation of the unit cell problem are discussed in details. Thereafter the in-plane material response is systematically characterized via homogenization analysis of the proposed special unit cell problem for different microstructures and the reasoning of the present method is justified. Moreover the present multiscale analysis procedure is illustrated through a plane stress beam example.

• 정보처리학회논문지C
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• 제9C권4호
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• pp.531-542
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• 2002

입력과 출력에 버퍼를 갖는 ATM 교환기의 셀 폐기 방법은 기존의 귀환(Backpressure)모드와 손실(Queueloss)모드가 있으며, 최근에는 두 모드의 단점을 보완한 하이브리드(Hybrid)모드가 제안되었다. 하이브리드모드는 목적하는 출력 버퍼와 입력 버퍼가 모두 포화일 경우에만 셀을 폐기하는 방식이다. 본 논문에서는 유니폼 트래픽하에서 Output-port expansion 기법을 사용한 귀환 손실 모드 및 하이브리드모드 하에서의 셀 손실률과 셀 지연을 성능 비교 분석한다 Output-port expansion 기법은, 한 타임 슬롯동안에 입력포트 당 하나의 셀만 교환되며, 만약 하나 이상의 셀들이 같은 출력포트로 향하고자 하면, 최대 교환되는 셀 수를 K(Output-port expansion ratio)개로 제한하는 방식이다. 셀 손실률을 비교 분석한 결과, 이전의 연구에서와는 달리 로드 0.9를 기점으로. 0.9이하의 로드에서는 하이브리드 모드가, 0.9 이상의 로드에서는 손실모드가 가장 낮은 셀 손실률을 보인다. 셀 지연을 비교 분석한 결과, 한 개의 교환기 성능 분석에서는 셀 손실로 인한 재전송(retransmission)을 고려하지 않는 관계로, 예상한 바와 같이, 로드가 많아질수록 셀 손실률이 높은 귀환모드가 K를 높일수록 다른 모드에 비해 낮은 셀 지연을 보였다.

• 한국통신학회논문지
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• 제37권8B호
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• pp.677-686
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• 2012

In the presence of a high power cellular network, picocells are added to a Macro-cell layout aiming to enhance total system throughput from cell-splitting. While because of the different transmission power between macrocell and picocell, and co-channel interference challenges between the existing macrocell and the new low power node-picocell, these problems result in no substantive improvement to total system effective throughput. Some works have investigated on these problems. Pico Cell Range Expansion (CRE) technique tries to employ some methods (such as adding a bias for Pico cell RSRP) to drive to offload some UEs to camp on picocells. In this work, we propose two solution schemes (including cell selection method, channel allocation and serving process) and combine new adaptive frequency partitioning reuse scheme to improve the total system throughput. In the simulation, we evaluate the performances of heterogeneous networks for downlink transmission in terms of channel utilization per cell (pico and macro), call blocking probability, outage probability and effective throughput. The simulation results show that the call blocking probability and outage probability are reduced remarkably and the throughput is increased effectively.

• Molecules and Cells
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• 제38권10호
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• pp.918-924
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• 2015

During T cell activation, mitochondrial content increases to meet the high energy demand of rapid cell proliferation. With this increase, the level of reactive oxygen species (ROS) also increases and causes the rapid apoptotic death of activated cells, thereby facilitating T cell homeostasis. Nicotinamide (NAM) has previously been shown to enhance mitochondria quality and extend the replicative life span of human fibroblasts. In this study, we examined the effect of NAM on $CD8^+$ T cell activation. NAM treatment attenuated the increase of mitochondrial content and ROS in T cells activated by CD3/CD28 antibodies. This was accompanied by an accelerated and higher-level clonal expansion resulting from attenuated apoptotic death but not increased division of the activated cells. Attenuation of ROS-triggered pro-apoptotic events and upregulation of Bcl-2 expression appeared to be involved. Although cells activated in the presence of NAM exhibited compromised cytokine gene expression, our results suggest a means to augment the size of T cell expansion during activation without consuming their limited replicative potential.

• 대한의생명과학회지
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• 제21권1호
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• pp.40-49
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• 2015

Mesenchymal stem cells (MSCs) have the ability to self-renew and differentiate into multi-lineage cells, thus highlighting the feasibility of using umbilical cord blood-derived MSCs (UCB-MSCs) for cell-therapy and tissueengineering. However, the low numbers of UCB-MSC derived from clinical samples requires that an ex vivo expansion step be implemented. As most stem cells reside in low oxygen tension environments (i.e., hypoxia), we cultured the UCBMSCs under 3% $O_2$ or 21% $O_2$ and the following parameters were examined: proliferation, senescence, differentiation and stem cell specific gene expression. UCB-MSCs cultured under hypoxic conditions expanded to significantly higher levels and showed less senescence compared to UCB-MSCs cultured under normoxic conditions. In regards to differentiation potential, UCB-MSCs cultured under hypoxic and normoxic conditions both underwent similar levels of osteogenesis as determined by ALP and von Kossa assay. Furthermore, UCB-MSCs cultured under hypoxic conditions exhibited higher expression of OCT4, NANOG and SOX2 genes. Moreover, cells expanded under hypoxia maintained a stem cell immnunophenotype as determined by flow cytometry. These results demonstrate that the expansion of human UCB-MSCs under a low oxygen tension microenvironment significantly improved cell proliferation and differentiation. These results demonstrate that hypoxic culture can be rapidly and easily implemented into the clinical-scale expansion process in order to maximize UCB-MSCs yield for application in clinical settings and at the same time reduce culture time while maintaining cell product quality.

• BMB Reports
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• 제54권7호
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• pp.374-379
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• 2021

Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling.

• Reproductive and Developmental Biology
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• 제31권4호
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• pp.267-272
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• 2007

Human embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the capacity to differentiate into various types of cells in the body. Hence, these cells may potentially be an indefinite source of cells for cell therapy in various degenerative diseases including neuronal disorders. For clinical applications of human ES cells, directed differentiation of these cells would be necessary. The objective of this study is to develop the culture condition for the expansion of neural precursor cells derived from human ES cells. Human ES cells were able to differentiate into neural precursor cells upon a stepwise culture condition. Neural precursor cells were propagated up to 5000-fold in cell numbers over 12-week period of culture and evaluated for their characteristics. Expressions of sox1 and pax6 transcripts were dramatically up-regulated along the differentiation stages by RT-PCR analysis. In contrast, expressions of oct4 and nanog transcripts were completely disappeared in neural precursor cells. Expressions of nestin, pax6 and sox1 were also confirmed in neural precursor cells by immunocytochemical analysis. Upon differentiation, the expanded neural precursor cells differentiated into neurons, astrocytes, and oligodendrocytes. In immunocytochemical analysis, expressions of type III ${\beta}$-tubulin and MAP2ab were observed Presence of astrocytes and oligodendrocytes were also confirmed by expressions of GFAP and O4, respectively. Results of this study demonstrate the feasibility of long-term expansion of human ES cell-derived neural precursor cells in vitro, which can be a potential source of the cells for the treatment of neurodegenerative disorders.