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http://dx.doi.org/10.5483/BMBRep.2021.54.7.024

Adipogenic function of tetranectin mediated by enhancing mitotic clonal expansion via ERK signaling  

Go, Seulgi (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University)
Park, Jihyun (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University)
Rahman, Safikur (Department of Botany, Munshi Singh College, BR Ambedkar Bihar University)
Jin, Juno (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University)
Choi, Inho (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University)
Kim, Jihoe (Department of Medical Biotechnology and Research Institute of Cell Culture, Yeungnam University)
Publication Information
BMB Reports / v.54, no.7, 2021 , pp. 374-379 More about this Journal
Abstract
Tetranectin (TN), an adipogenic serum protein, enhances adipocyte differentiation, however, its functional mechanism has yet to be elucidated. In the present study, we investigated the adipogenic function of TN by using medium containing TN-depleted fetal bovine serum (TN-del-FBS) and recombinant mouse TN (mTN). The adipocyte differentiation of 3T3-L1 cells was significantly enhanced by mTN supplementation essentially at differentiation induction, which indicated a potential role of the protein in the early differentiation phase. The adipogenic effect of mTN was more significant with insulin in the differentiation induction cocktail, implicating their close functional relationship. mTN enhanced not only the proliferation of growing cells, but also mitotic clonal expansion (MCE) that is a prerequisite for adipocyte differentiation in the early phase. Consistently, mTN increased the phosphorylation of ERK in the early phase of adipocyte differentiation. Results of this study demonstrate that the adipogenic function of mTN is mediated by enhancing MCE via ERK signaling.
Keywords
Adipocyte differentiation; ERK; Mitotic clonal expansion; Serum protein; Tetranectin;
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Times Cited By KSCI : 2  (Citation Analysis)
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