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http://dx.doi.org/10.14348/molcells.2015.0168

High-Dose Nicotinamide Suppresses ROS Generation and Augments Population Expansion during CD8+ T Cell Activation  

Choi, Ho Jin (Department of Life Science, University of Seoul)
Jang, So-Young (Department of Life Science, University of Seoul)
Hwang, Eun Seong (Department of Life Science, University of Seoul)
Publication Information
Molecules and Cells / v.38, no.10, 2015 , pp. 918-924 More about this Journal
Abstract
During T cell activation, mitochondrial content increases to meet the high energy demand of rapid cell proliferation. With this increase, the level of reactive oxygen species (ROS) also increases and causes the rapid apoptotic death of activated cells, thereby facilitating T cell homeostasis. Nicotinamide (NAM) has previously been shown to enhance mitochondria quality and extend the replicative life span of human fibroblasts. In this study, we examined the effect of NAM on $CD8^+$ T cell activation. NAM treatment attenuated the increase of mitochondrial content and ROS in T cells activated by CD3/CD28 antibodies. This was accompanied by an accelerated and higher-level clonal expansion resulting from attenuated apoptotic death but not increased division of the activated cells. Attenuation of ROS-triggered pro-apoptotic events and upregulation of Bcl-2 expression appeared to be involved. Although cells activated in the presence of NAM exhibited compromised cytokine gene expression, our results suggest a means to augment the size of T cell expansion during activation without consuming their limited replicative potential.
Keywords
mitochondria; nicotinamide; ROS; T cell activation;
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