• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2004년도 추계학술대회 논문집
• /
• pp.1215-1220
• /
• 2004

Cell adhesion to any material surface is considered to be fundamental and important phenomenon in the fields of tissue engineering. Cell adhesion molecules, mechanism, and attachment force have been studied and described a lot. However, the effects of mechanical stimuli on the adhesive forces still have been left much to be investigated. In this study, to investigate the changes in cell adhesive force due to resting time period during the intermittent hydrostatic pressurizing (IHP), cells were cultured under the IHP with various resting times. Then the cell adhesive forces were measured quantitatively utilizing a cell detachment test system and immunofluorescent staining was performed using fluorescent microscopy. In the results, immediately after mechanical stimuli (150 minutes after seeding) and one hour later (210 minutes after seeding), the average adhesive force of experimental group 5 (resting time: 15min) compared with that of control group at same culture time was increased significantly (p<0.05). The results indicated that IHP can contribute in improving cell adhesive force and some of time intervals were required for the expression of cell response.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2005년도 추계학술대회 논문집
• /
• pp.69-72
• /
• 2005

In this study, effects of IHPs with various resting times to cell adhesion were investigated through the measurements of cell adhesive force, number and area of focal contacts (stained vinculin spots), and projected cell area, perimeter and circularity. In addition correlation tests and curve estimations using the experimental results were performed fur the finding an essential factor for increment of cell adhesive force. Tn the results, immediately after mechanical stimuli (150 minutes after seeding) and one hour later (210 minutes after seeding), the average adhesive force of experimental group 5 (resting time: 15min) compared with that of control group at same culture time was increased significantly (p<0.05). Average projected area and perimeter of cells at Group 5 were increased significantly (p<0.05), while average circularity of cells at Group 5 incubated fur 210 minutes was decreased significantly (p<0.05). In the digital image analysis of focal contacts containing vinculins, area and numbers of focal contacts per cell at Group 5 were higher than those of the other groups. This study indicated that IHP with appropriate resting time could contribute in improving cell adhesive force, cell spreading, development of cytoskeleton and formation of focal contacts. And cell adhesive force was correlated to the morphological aspects of cell and development of focal contacts. Particularly, area of focal contacts was closely related to cell adhesive force.

• The Journal of Advanced Prosthodontics
• /
• 제4권2호
• /
• pp.97-102
• /
• 2012

Study was conducted to determine and assess the effect of different type of denture adhesives on the incisal bite force of complete denture wearers until the dislodgement of upper denture, using pressure transducer. MATERIALS AND METHODS. 30 patients out of 100 were included in the study. Based on the Kapur's method of scoring denture retention and stability, these patients were divided into 3 groups-Group A - Clinically good dentures; Group B - Clinically fair dentures; and Group C - Clinically poor dentures. A custom made occlusal force meter was constructed based on the load cell type of pressure transducers. Different adhesives (powder, paste and adhesive strips) were used in the study. Complete denture wearers were asked to bite on the load cell and the readings of incisal bite force were recorded. The readings of incisal bite force were subjected to statistical analysis using Repeated measures ANOVA followed by post-hoc bonferroni test. RESULTS. The result suggests that denture adhesives improved the incisal bite force of complete denture wearers significantly The incisal bite force (in kg) in Group A without using adhesives, with powder adhesive, with paste adhesive and with adhesive strips was found to be 2.48 (${\pm}0.16$), 3.43 (${\pm}0.11$), 6.01 (${\pm}0.11$), 3.22 (${\pm}0.09$) respectively. The incisal bite force (in kg) in Group B without using adhesives, with powder adhesive, with paste adhesive and with adhesive strips was found to be 1.87 (${\pm}0.18$), 3.35 (${\pm}0.14$), 5.34 (${\pm}0.18$), 3.21 (${\pm}0.12$) respectively. The incisal bite force (in kg) in Group C without using adhesives, with powder adhesive, with paste adhesive and with adhesive strips was found to be 1.00 (${\pm}0.17$), 3.07 (${\pm}0.14$), 4.37 (${\pm}0.26$), 2.99 (${\pm}0.14$) respectively. CONCLUSION. Within the limitations of the study, it was concluded that the use of denture adhesive was found to be significantly effective in improving the incisal bite force of complete dentures until the dislodgement of upper denture. Fittydent paste adhesive was found to be more effective than the powder and strips adhesives. The improvement in incisal bite force was found to be higher in Group C in comparison to that of Group A and Group B.

• 한국정밀공학회:학술대회논문집
• /
• 한국정밀공학회 2000년도 추계학술대회 논문집
• /
• pp.635-638
• /
• 2000

It is necessary to work on a vertical plane of workpiece in order to produce a large structure like a ship. These works can be automated by using the robot with permanent magnet wheels. We developed the permanent magnet wheel which can be used by a mobile robot and easily detached. We enhanced an adhesive power by restricting the occurrence direction of magnetic flow. And we also developed a method which weakens adhesive magnetic force by changing magnetic flow with metal pins. We used the load cell and the gaussmeter to measure the characteristics of the adhesive force and magnetic force. We obtained the result that the adhesive power is reduced to 1/3 of normal state by using 4 inducing pins.

• Tribology and Lubricants
• /
• 제21권5호
• /
• pp.236-240
• /
• 2005

In order to prepare for the suitable surfaces of implants or medical devices, quantitative evaluation of adhesion between cells and biomaterials is essential. To better understand adhesion formation between cells and biomaterials, we used the cytodetachment technique which measures the adhesive force of a single cell through changing the, culture time and detachment speed. The results showed that the adhesive force could be affected by the culture time of cells on the surface of materials and the detachment speed. Moreover, there was a large discrepancy among the adhesion strength measured by similar techniques conducted on the different cells and substrates. It can be 'concluded that the variation of the force measurement technique can seriously alter the level of the force required to detach a cell on the surface of materials.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회A
• /
• pp.1580-1581
• /
• 2008

We investigated the effects of intermittent hydrostatic pressure with various duration of resting period on changes in calcium ($Ca^{2+}$) concentration and adhesive forces of cells on substrates. The quantitive adhesive forces of cells were measured under various resting periods. When the pressure applied to the cells, the concentration of $Ca^{2+}$ increased. Under intermittent hydrostatic pressure, the concentration of $Ca^{2+}$ was maintained under a resting period of 15 min, while it was not decreased with other resting periods of less than 15 min. With a resting period of 15 min, the magnitudes of adhesive forces were significantly increase. In addition, the adhesive forces were measured with and without $Ca^{2+}$ chelating agents to evaluate the effect of $Ca^{2+}$ on cell adhesiveness. When $Ca^{2+}$ ions were chelated, the adhesive forces dramatically decreased, even under intermittent hydrostatic pressure. We conclude that $Ca^{2+}$ plays an crucial role in modulating the adhesive forces of cells, and that the concentration of $Ca^{2+}$ can be increased by intermittent hydrostatic stimuli.

• 한국윤활학회:학술대회논문집
• /
• 한국윤활학회 2004년도 학술대회지
• /
• pp.362-366
• /
• 2004

Quantitative evaluation of substrates for cells is essential to understanding cell-material adhesive interaction and it is also necessary for the development of new biomaterials. Many cells on adhesive molecules will form an organization of actin into bundles and production of the large, highly organized structures termed focal adhesions. To better understand adhesion formations between cells and substrata, we have quantified the force required to displace attached cell. we allowed rabbit knee chondrocyte to attach on a substratum of microscope slide glass. Our results demonstrate that a force is required to detach cells is changed according to detachment time variation.

• 한국컴퓨터정보학회논문지
• /
• 제22권10호
• /
• pp.1-7
• /
• 2017

In this paper, the separation force measuring system is developed. The separation force aries due to adhesive strength between semiconductor epoxy molding compound(EMC) and the metal plate in semiconductor formed plate. In general, when removing the metal plate in semiconductor formed plate from semiconductor epoxy molding compound, excessive strength can result in a increase in semiconductor defect rates, or conversely, if too little force is exerted on the metal plate in semiconductor formed plate, the semiconductor production rates can decrease. In this study, the design criteria for the selection of the AC servo motor, the role of the ball screw, the relationship between the load cell and the ball screw, and the rate of deceleration are given. In addition, minimizing the reject rate of semiconductors and maximizing the semiconductor production rate are achieved through the standardization of the collected separation force data measured by the proposed system.

• Macromolecular Research
• /
• 제17권7호
• /
• pp.458-463
• /
• 2009

Polyurethane (PU) is widely used as a cardiovascular biomaterial due to its good mechanical properties and hemocompatibility, but it is not adhesive to endothelial cells (ECs). Cell adhesive peptides, GRGDS and YIGSR, were found to promote adhesion and spreading of ECs and showed a synergistic effect when both of them were used. In this study, a surface modification was designed to fabricate an EC-active PU surface capable of promoting endothelialization using the peptides and poly(ethylene glycol) (PEG) spacer, The modified PU surfaces were characterized in vitro. The density of the grafted PEG on the PU surface was measured by acid-base back titration to the terminal-free isocyanate groups. The successful immobilization of pep tides was confirmed by amino acid analysis, following hydrolysis, and contact angle measurement. The uniform distribution of peptides on the surface was observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). To evaluate the EC adhesive property, cell viability test using human umbilical vein EC (HUVEC) was investigated in vitro and enhanced endothelialization was characterized by the introduction of cell adhesive peptides, GRGDS and YIGSR, and PEG spacer. Therefore, GRGDS and YIGSR co-immobilized PU surfaces can be applied to an EC-specific vascular graft with long-term patency by endothelialization.

• 한국기계가공학회지
• /
• 제13권6호
• /
• pp.30-36
• /
• 2014

Many different cells types have been found to be highly sensitive to mechanical force imposed by their surroundings. The cellular response to external mechanical forces has very important effects on numerous biological phenomena. In spite of its importance in biological processes, the cell adhesion force remains difficult to measure quantitatively at the cellular level. In this paper, to enhance quantitative measurements of cell adhesive interactions, a new attaching system and a method in which a glass bead can be attached to an AFM cantilever was designed and fabricated, and the degree of range displacement was controlled in the system. In an experiment, the movement of the stage in the attaching system and the attaching process were measured. The effectiveness of this system was confirmed as well in the experiment. In addition, through a commercial AFM system, the spring constant of the modified AFM probe could be measured.