• Title/Summary/Keyword: Cell Observation

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The Study of Efficiency by Palmarosa, Neroli & Jasmin Essential Oil on Dry Skin Induced by Surfactant (계면활성제 유도 건성피부에 대한 Palmarosa, Neroli & Jasmin blending Oil의 유효성 비교 연구)

  • Jung, Hyun-Mee;Choi, Jeung-Sook
    • Fashion & Textile Research Journal
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    • v.9 no.5
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    • pp.569-572
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    • 2007
  • The effectiveness of Palmarosa, Neroli and Jasmin blending oil on dry skin of rat induced by kitchen detergent are investigated. The experimental groups were divided the control group, group treated with surfactant, group treated with Palmarosa and Neroli, and group with Palmarosa, Neroli and Jasmin. Observation of epidermis and the alteration of mast cell were performed with photomicroscope. According to the epidermis morphological changes analysis, the A3 group treated with Palmarosa, Neroli and Jasmin blending oil was appeared the most similar with the control group, and the A2 group applied with Palmarosa and Neroli blending oil was sequently displayed similar characteristics. The collagen layer's breakaway resulting from Palmarosa essential oil, the collagen layer's restoration resulting from Neroli essential oil, the collagen layer's retention hyperkeratosis resulting from Jasmin essential oil were observed in the structure of the epidermal layer. In photomicrosope observation of mast cell to examine the inflammatory reactions, the increase in size and number of mast cell were showed in A1 group treated with surfactant compared to the control group. The number of mast cells definitely decreased in groups which were treated with Palmarosa, Neroli and Jasmin blending oil.

Histological Observation of Embryogenic and Non-embryogenic Callus in Long-term Subculture of Wild Viola (Viola patrinii DC.) (흰제비꽃 배양세포에 있어서 분화세포와 미분화세포 조직의 비교 관찰)

  • 정용모;손병구;이재헌;서정해;정정한;권오창
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.233-238
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    • 2000
  • To obtain a basic information of the development of Genus Viola, morphological and histological observation of in vitro calli and cells in Viola culture cells were investigated. There were two callus types obtained by long term subculture of wild viola (Viola partrinii DC. ) petiole callus. One was friable callus - soft and pale green in color and small cells in size, and the other was compact callus - compact and deep bluish green in color, large cells in size. In scanning electron microscopic observation, friable callus was composed of voculated cell around small. cell clump, while compact callus was composed of cells filled with protoplasm Somatic embryogenesis was observed from suspension culture of the compact callus.

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SEM Observation for the Damage of Inner Hair Cell Stereocilia of Guinea Pig Cochlea after Loud Tone Exposure (격음노출 후 기니픽 달팽이관 내유모세포 부동섬모에 관한 SEM(전자투사식현미경) 관측)

  • Jarng Soon Suck
    • The Journal of the Acoustical Society of Korea
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    • v.24 no.1E
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    • pp.1-6
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    • 2005
  • The inner hair cell stereocilia of the guinea pig cochlea was examined under a scanning electron microscope (SEM) after loud tone exposure onto the ear drum of the animal. Before and after guinea pigs were exposed to intensive and continuous tone such as 106 dB SPL in intensity, the functioning of the cochlea was monitored by N1-N2 audiograms. The structural damage of the stereocilia of inner hair cells (IHCs) and outer hair cells (OHCs) was examined using the SEM in x 1500 magnification. The comparison between the functional change of the cochlea and the structural damage of the IHC stereocilia is done by means of photographic observation. It can be shown that the functional change might be related to the structural damage of the IHC stereocilia after intensive acoustic trauma.

A study On An Identification of Interactions In A Nonreplicated Two-Way Layout With $L_1$-Estimation

  • Lee, Ki-Hoon
    • Communications for Statistical Applications and Methods
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    • v.7 no.1
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    • pp.119-128
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    • 2000
  • This paper proposes a method for detecting interactions in a two-way layout with one observation per cell. The identification of interactions in the model is not clear for they are confounding with error terms. The $L_1$-Estimation is robust with respect to a y-direction outlier in linear model so we are able to estimate main effects without affection of interactions, If an observation is classified as an outlier we conclude it contains an interaction. An empirical study compared with a classical method is performed.

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Effects of Bypass Diode Array Configurations on Solar System (태양광시스템에서 바이패스 다이오드 배열의 영향도 분석)

  • Park, Soyoung;Lee, Jonghwan
    • Journal of the Semiconductor & Display Technology
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    • v.21 no.1
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    • pp.127-131
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    • 2022
  • The effect of bypass diodes on the output energy of solar cells was investigated under the condition of partial shading. The maximum power point was estimated using the perturbation & observation algorithm, taking into account the correlation effect between the arrangement and number of bypass diodes. The performance of the bypass diode was tested under the consideration of the partial shading effect and simulated using a Matlab/Simulink.

3 차원 주화성 모델 개발을 통한 흡착형 세포의 동적특성 연구

  • Song, Ji-Hwan;Kim, Dong-Choul
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.1638-1642
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    • 2008
  • Cell migration is one of the essential mechanisms responsible for complex biological processes. Intensive researches have begun to elucidate the mechanisms and search intriguing conditions for efficient control of cell migration. One general mechanism which is widely applicable for cells including neutrophil, Escherichia coli and endothelial cell is chemotaxis. Especially, understanding the chemotactic mechanics of cell crawling has important implications for various medical and biological applications. The single cell study for chemotaxis has an advantage over studies with the population of cells in providing a clearer observation of cell migration, which leads to more accurate assessments of chemotaxis. In this paper, we propose a three-dimensional model considering a single crawling cell to study its chemotaxis. The semi-implicit Fourier spectral method is applied for high efficiency and numerical stability. The simulation results reveal rich dynamics of cell.

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The Cell Surface Expression of H2-M3 Does Not Directly Effect on the Killing Activity of NK Cell (H2-M3의 세포 표면 발현이 NK 세포의 활성에 미치는 영향 분석)

  • Lee, Sang-Yeol;Chun, Tae-Hoon
    • YAKHAK HOEJI
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    • v.53 no.3
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    • pp.125-129
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    • 2009
  • H2-M3 (M3) is a unique antigen presenting molecule which provides N-formylated peptide to certain type of T cells. Previous observation indicated that NK cell activity is significantly diminished during listerial infection in $H2-M3^{-/-}$ mice. To explore the possibility that M3 expression directly effect on NK cell activity, we measured NK cell activity with or without stimulation of N-formylated peptide on antigen presenting cells. Results indicated that the expression of M3 is not directly influence on NK cell activity. Further study will be focused on the indirect effect of M3 on regulating NK cell activity.

Basal cell nevus syndrome with excessive basal cell carcinomas

  • Kim, Choon Soo;Na, Young Cheon
    • Archives of Craniofacial Surgery
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    • v.22 no.2
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    • pp.122-125
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    • 2021
  • Basal cell nevus syndrome (BCNS), also known as basal cell carcinoma nevus syndrome, Gorlin syndrome, Gorlin-Goltz syndrome, and nevoid basal cell carcinoma, is a rare autosomal dominant disorder with a prevalence of approximately 1/60,000. A lower prevalence rate of 1/13,939,393 has also been reported in Korea. We report the case of a 40-year-old male patient with multiple black pigmented macules on the face that first appeared when he was a teenager. His clinical features of jaw cysts, bifid ribs, and calcification of the falx cerebri were fitting within the criteria for the diagnosis of BCNS. We excised all suspected macules and sent permanent biopsy. Most of the histological examinations of the biopsy samples taken during surgical excision of the face masses showed basal cell carcinomas. Ten months after the surgery, the patient has remained free from symptoms and is undergoing follow-up observation.

An Immune-Electron Microscopic Study of the Apoptotic Cell during Mouse Knee Joint Development (생쥐 무릎관절 공간 발생에 있어 아포프토시스 세포에 관한 면역전자현미경적 연구)

  • Chae, Hee-Sun;Kim, Kyung-Yong;Lee, Won-Bok;Lim, Hyoung-Soo;Hwang, Douk-Ho;Chang, Ka-Yong
    • Applied Microscopy
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    • v.28 no.1
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    • pp.107-119
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    • 1998
  • This study was designed to investigate the appearence and the characteristics of the apoptotic cells and the process of the joint cavity formation in mouse knee joint. Fetal mouse knee joints from 15 to 19 days of gestation were used. Paraffin-embedded serial sections, stained with H & E for light microscopic observation, Epon 812 embedded thin sections for electron microscopic observation and Lowicryl HM 20 embedded thin sections for immune-electron microscopic observation were prepared. Monoclonal antibodies to $\beta-tubulin$ and polyclonal antibodies to tissue transglutaminase were used for immune-electron microscopic study. The results obtained were as follows. 1. At 15 days of gestation, blood vessels, which have invaded in the mesenchymal cells, were present in the synovium, to form the joint cavity in the future. 2. At 16 days of gestation, the joint cleft was first appeared and several RBCs were present in the joint cleft. The invasion of blood vessels into the joint cleft was continuing, and apoptotic cells were present in the inner cell layer, adjacent to the joint cleft. Necrotic cells were also present in the outer cell layer; they were present 18 days of gestation, but apoptotic cells did not appear after 17 days of gestation. 3. In the apoptotic cells, transglutaminase were localized around vacuoles and the marginal site of the cytoplasm. 4. In the apoptotic cells, tubulin was around the endoplasmic reticulum and the marginal site of the cytoplasm. In the late stage of apoptotic cells, tubulin was localized diffusely in the cytoplasm. Tubulin was also strongly labeled around in the cytoplasm of the neighboring cell at which the apoptotic body was phagocytosed. Tubulin labeled particles were apparently increased in the seperated apoptotic bodies. On the basis of the above findings, it is proposed that during the development of the mouse knee joint, blood vessel invasion first occurs and then apoptosis and cell necrosis follow it. In the apoptotic cell, present in the synovium of the developing knee joint of the mouse. it is suggested that the redistribution of tubulin is associated with apoptotic process. And transglutaminase overexpressed in the apoptotic cell.

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Anti-Cancer Effects of Salvia Miltiorrhiza, Carydalis Turtschaminovii, Reynoutria Elliptica Herbal Acupuncture on Solid Tumor of Rats induced by Injection of RK3E-ras Cells (RK3E-ras cells로 유발된 흰쥐의 고형종양에 대한 단삼, 현호색, 호장근 약침의 항종양 효과)

  • Park, Soo-Gon;Shin, Mi-Suk;Choi, Jin-Bong;Kim, Sun-Jong
    • Journal of Korean Medicine Rehabilitation
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    • v.19 no.1
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    • pp.91-102
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    • 2009
  • Objectives : The present study was carried out investigate the anti-cancer effect of Salvia miltiorrhiza, Carydalis turtschaminovii and Reynoutria elliptica herbal acupuncture on solid tumor of rats induced by injection of RK3E-ras cells. Methods : RK3E-ras cells were injected on the right lumbar region of rats. After 1 weeks, the experimental rats were divided into four groups : Control group, Salvia miltiorrhiza herbal acupuncture group(SM), Carydalis turtschaminovii herbal acupuncture group(CT), Reynoutria elliptica herbal acupuncture group(RE). And we investigated the weight and size of tumor tissue, gross anatomy, histological and PCNA immunohistochemical study, hepatic and renal metastasis for tumor of each group. Results : 1. In the weight of tumor tissue assessment, SM and CT's weight of tumor tissue was decreased. 2. In the size of tumor tissue assessment, SM was smaller than any other group. 3. In the histological observation, SM's formation of tunica fibrosa that surround the tumor cell was obvious and vasculature that developes circumference of tumor cell was not observed, and density of tumor cell was very low. 4. In the PCNA immunohistochemical study, Control group, SM, RE showed strong immune response in the central site of tumor tissue. 5. In observation of liver and kidney tissue, we were not able to observe tumor cell in the SM. Conclusions : Consequently, SM and CT showed a inhibition of growth and metastasis.