• 제목/요약/키워드: Cell Observation

검색결과 913건 처리시간 0.027초

계면활성제 유도 건성피부에 대한 Palmarosa, Neroli & Jasmin blending Oil의 유효성 비교 연구 (The Study of Efficiency by Palmarosa, Neroli & Jasmin Essential Oil on Dry Skin Induced by Surfactant)

  • 정현미;최정숙
    • 한국의류산업학회지
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    • 제9권5호
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    • pp.569-572
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    • 2007
  • The effectiveness of Palmarosa, Neroli and Jasmin blending oil on dry skin of rat induced by kitchen detergent are investigated. The experimental groups were divided the control group, group treated with surfactant, group treated with Palmarosa and Neroli, and group with Palmarosa, Neroli and Jasmin. Observation of epidermis and the alteration of mast cell were performed with photomicroscope. According to the epidermis morphological changes analysis, the A3 group treated with Palmarosa, Neroli and Jasmin blending oil was appeared the most similar with the control group, and the A2 group applied with Palmarosa and Neroli blending oil was sequently displayed similar characteristics. The collagen layer's breakaway resulting from Palmarosa essential oil, the collagen layer's restoration resulting from Neroli essential oil, the collagen layer's retention hyperkeratosis resulting from Jasmin essential oil were observed in the structure of the epidermal layer. In photomicrosope observation of mast cell to examine the inflammatory reactions, the increase in size and number of mast cell were showed in A1 group treated with surfactant compared to the control group. The number of mast cells definitely decreased in groups which were treated with Palmarosa, Neroli and Jasmin blending oil.

흰제비꽃 배양세포에 있어서 분화세포와 미분화세포 조직의 비교 관찰 (Histological Observation of Embryogenic and Non-embryogenic Callus in Long-term Subculture of Wild Viola (Viola patrinii DC.))

  • 정용모;손병구;이재헌;서정해;정정한;권오창
    • 식물조직배양학회지
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    • 제27권3호
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    • pp.233-238
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    • 2000
  • 야생 흰제비꽃의 엽병 유래 callus를 장기 계대배양하는 과정 중에 발생하는 순화된 friable callus와 분화능이 높은 compact callus를 비교 관찰한 바, friable callus는 연초록색으로 부서지기 쉬운 부드러운 callus이고, compact callus는 진녹색으로 단단한 callus였다. 동결처리 한 시료를 주사전자 현미경에서 동일하게 200배로 관찰하여 보면, friable callus 는 작은 세포집단으로 이루어진 세포군의 주변부에 고도로 액포화된 세포가 광범위하게 분포되어 있는 반면, compact callus는 거의 균일한 세포들로 구성되어 세포구성이 치밀한 것으로 관찰되었다. 또한 friable callus와 compact callus로 부터의 체세포배형성은 배양세포에서 배가 발생하여 배양기간이 지남에 따라 식물체로 분화하였다. 이와 같은 과정은 배양세포의 세포질이 보다 충만한 부위에서 배유사체 (embryo like body)의 발생이 이루어지는 것으로 관찰되었다.

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격음노출 후 기니픽 달팽이관 내유모세포 부동섬모에 관한 SEM(전자투사식현미경) 관측 (SEM Observation for the Damage of Inner Hair Cell Stereocilia of Guinea Pig Cochlea after Loud Tone Exposure)

  • Jarng Soon Suck
    • The Journal of the Acoustical Society of Korea
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    • 제24권1E호
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    • pp.1-6
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    • 2005
  • The inner hair cell stereocilia of the guinea pig cochlea was examined under a scanning electron microscope (SEM) after loud tone exposure onto the ear drum of the animal. Before and after guinea pigs were exposed to intensive and continuous tone such as 106 dB SPL in intensity, the functioning of the cochlea was monitored by N1-N2 audiograms. The structural damage of the stereocilia of inner hair cells (IHCs) and outer hair cells (OHCs) was examined using the SEM in x 1500 magnification. The comparison between the functional change of the cochlea and the structural damage of the IHC stereocilia is done by means of photographic observation. It can be shown that the functional change might be related to the structural damage of the IHC stereocilia after intensive acoustic trauma.

A study On An Identification of Interactions In A Nonreplicated Two-Way Layout With $L_1$-Estimation

  • Lee, Ki-Hoon
    • Communications for Statistical Applications and Methods
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    • 제7권1호
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    • pp.119-128
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    • 2000
  • This paper proposes a method for detecting interactions in a two-way layout with one observation per cell. The identification of interactions in the model is not clear for they are confounding with error terms. The $L_1$-Estimation is robust with respect to a y-direction outlier in linear model so we are able to estimate main effects without affection of interactions, If an observation is classified as an outlier we conclude it contains an interaction. An empirical study compared with a classical method is performed.

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태양광시스템에서 바이패스 다이오드 배열의 영향도 분석 (Effects of Bypass Diode Array Configurations on Solar System)

  • 박소영;이종환
    • 반도체디스플레이기술학회지
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    • 제21권1호
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    • pp.127-131
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    • 2022
  • The effect of bypass diodes on the output energy of solar cells was investigated under the condition of partial shading. The maximum power point was estimated using the perturbation & observation algorithm, taking into account the correlation effect between the arrangement and number of bypass diodes. The performance of the bypass diode was tested under the consideration of the partial shading effect and simulated using a Matlab/Simulink.

3 차원 주화성 모델 개발을 통한 흡착형 세포의 동적특성 연구

  • 송지환;김동철
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2008년도 추계학술대회A
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    • pp.1638-1642
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    • 2008
  • Cell migration is one of the essential mechanisms responsible for complex biological processes. Intensive researches have begun to elucidate the mechanisms and search intriguing conditions for efficient control of cell migration. One general mechanism which is widely applicable for cells including neutrophil, Escherichia coli and endothelial cell is chemotaxis. Especially, understanding the chemotactic mechanics of cell crawling has important implications for various medical and biological applications. The single cell study for chemotaxis has an advantage over studies with the population of cells in providing a clearer observation of cell migration, which leads to more accurate assessments of chemotaxis. In this paper, we propose a three-dimensional model considering a single crawling cell to study its chemotaxis. The semi-implicit Fourier spectral method is applied for high efficiency and numerical stability. The simulation results reveal rich dynamics of cell.

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H2-M3의 세포 표면 발현이 NK 세포의 활성에 미치는 영향 분석 (The Cell Surface Expression of H2-M3 Does Not Directly Effect on the Killing Activity of NK Cell)

  • 이상열;전태훈
    • 약학회지
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    • 제53권3호
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    • pp.125-129
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    • 2009
  • H2-M3 (M3) is a unique antigen presenting molecule which provides N-formylated peptide to certain type of T cells. Previous observation indicated that NK cell activity is significantly diminished during listerial infection in $H2-M3^{-/-}$ mice. To explore the possibility that M3 expression directly effect on NK cell activity, we measured NK cell activity with or without stimulation of N-formylated peptide on antigen presenting cells. Results indicated that the expression of M3 is not directly influence on NK cell activity. Further study will be focused on the indirect effect of M3 on regulating NK cell activity.

Basal cell nevus syndrome with excessive basal cell carcinomas

  • Kim, Choon Soo;Na, Young Cheon
    • 대한두개안면성형외과학회지
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    • 제22권2호
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    • pp.122-125
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    • 2021
  • Basal cell nevus syndrome (BCNS), also known as basal cell carcinoma nevus syndrome, Gorlin syndrome, Gorlin-Goltz syndrome, and nevoid basal cell carcinoma, is a rare autosomal dominant disorder with a prevalence of approximately 1/60,000. A lower prevalence rate of 1/13,939,393 has also been reported in Korea. We report the case of a 40-year-old male patient with multiple black pigmented macules on the face that first appeared when he was a teenager. His clinical features of jaw cysts, bifid ribs, and calcification of the falx cerebri were fitting within the criteria for the diagnosis of BCNS. We excised all suspected macules and sent permanent biopsy. Most of the histological examinations of the biopsy samples taken during surgical excision of the face masses showed basal cell carcinomas. Ten months after the surgery, the patient has remained free from symptoms and is undergoing follow-up observation.

생쥐 무릎관절 공간 발생에 있어 아포프토시스 세포에 관한 면역전자현미경적 연구 (An Immune-Electron Microscopic Study of the Apoptotic Cell during Mouse Knee Joint Development)

  • 채희선;김경용;이원복;임형수;황덕호;장가용
    • Applied Microscopy
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    • 제28권1호
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    • pp.107-119
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    • 1998
  • This study was designed to investigate the appearence and the characteristics of the apoptotic cells and the process of the joint cavity formation in mouse knee joint. Fetal mouse knee joints from 15 to 19 days of gestation were used. Paraffin-embedded serial sections, stained with H & E for light microscopic observation, Epon 812 embedded thin sections for electron microscopic observation and Lowicryl HM 20 embedded thin sections for immune-electron microscopic observation were prepared. Monoclonal antibodies to $\beta-tubulin$ and polyclonal antibodies to tissue transglutaminase were used for immune-electron microscopic study. The results obtained were as follows. 1. At 15 days of gestation, blood vessels, which have invaded in the mesenchymal cells, were present in the synovium, to form the joint cavity in the future. 2. At 16 days of gestation, the joint cleft was first appeared and several RBCs were present in the joint cleft. The invasion of blood vessels into the joint cleft was continuing, and apoptotic cells were present in the inner cell layer, adjacent to the joint cleft. Necrotic cells were also present in the outer cell layer; they were present 18 days of gestation, but apoptotic cells did not appear after 17 days of gestation. 3. In the apoptotic cells, transglutaminase were localized around vacuoles and the marginal site of the cytoplasm. 4. In the apoptotic cells, tubulin was around the endoplasmic reticulum and the marginal site of the cytoplasm. In the late stage of apoptotic cells, tubulin was localized diffusely in the cytoplasm. Tubulin was also strongly labeled around in the cytoplasm of the neighboring cell at which the apoptotic body was phagocytosed. Tubulin labeled particles were apparently increased in the seperated apoptotic bodies. On the basis of the above findings, it is proposed that during the development of the mouse knee joint, blood vessel invasion first occurs and then apoptosis and cell necrosis follow it. In the apoptotic cell, present in the synovium of the developing knee joint of the mouse. it is suggested that the redistribution of tubulin is associated with apoptotic process. And transglutaminase overexpressed in the apoptotic cell.

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RK3E-ras cells로 유발된 흰쥐의 고형종양에 대한 단삼, 현호색, 호장근 약침의 항종양 효과 (Anti-Cancer Effects of Salvia Miltiorrhiza, Carydalis Turtschaminovii, Reynoutria Elliptica Herbal Acupuncture on Solid Tumor of Rats induced by Injection of RK3E-ras Cells)

  • 박수곤;신미숙;최진봉;김선종
    • 한방재활의학과학회지
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    • 제19권1호
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    • pp.91-102
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    • 2009
  • Objectives : The present study was carried out investigate the anti-cancer effect of Salvia miltiorrhiza, Carydalis turtschaminovii and Reynoutria elliptica herbal acupuncture on solid tumor of rats induced by injection of RK3E-ras cells. Methods : RK3E-ras cells were injected on the right lumbar region of rats. After 1 weeks, the experimental rats were divided into four groups : Control group, Salvia miltiorrhiza herbal acupuncture group(SM), Carydalis turtschaminovii herbal acupuncture group(CT), Reynoutria elliptica herbal acupuncture group(RE). And we investigated the weight and size of tumor tissue, gross anatomy, histological and PCNA immunohistochemical study, hepatic and renal metastasis for tumor of each group. Results : 1. In the weight of tumor tissue assessment, SM and CT's weight of tumor tissue was decreased. 2. In the size of tumor tissue assessment, SM was smaller than any other group. 3. In the histological observation, SM's formation of tunica fibrosa that surround the tumor cell was obvious and vasculature that developes circumference of tumor cell was not observed, and density of tumor cell was very low. 4. In the PCNA immunohistochemical study, Control group, SM, RE showed strong immune response in the central site of tumor tissue. 5. In observation of liver and kidney tissue, we were not able to observe tumor cell in the SM. Conclusions : Consequently, SM and CT showed a inhibition of growth and metastasis.