• Journal of Auto-vehicle Safety Association
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• v.10 no.1
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• pp.20-26
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• 2018

The fatality of rollover accidents in motor vehicle crashes is high despite their low incidence. Through the investigation of a 12-passenger van rollover accident in which 10 passengers were involved, we intend to analyze the correlation between the severity of the injury and the position of the occupants. We collected accident information from medical records, interviews, photo-images of the damaged van, field surveys, and the results of the Korean New Car Assessment Program (KNCAP). Based on the occupants' position, we classified injury sites and estimated injury severity. Passenger injury severity was evaluated by trauma score calculation. The initiation type of the rollover accident was passenger side 'fall-over' and the Collision Deformation Classification (CDC) code for the damaged van was 00TDZO3. The crash of the van involved 10 passengers, with an average age of $16.3{\pm}4.2years$. Few of the occupants had fastened seat belts at the time of the incident, and there was no airbag installed. One patient sustained severe liver injury and another was diagnosed with a fracture of the right humerus. The most common injuries were at the upper extremities and the neck. The average of Injury Severity Score (ISS) was $4.8{\pm}5.9$, and the average ISS of right-seated, mid-seated and left-seated occupants was $7.5{\pm}9.3$, $1.5{\pm}0.7$, and $3.3{\pm}2.1$ respectively (p>0.05). In the rollover (to-passenger side) accident of occupant unfastened, the average ISS of right-seated occupants (near side) was higher, but there was no statistically significant difference.

• 한국태양에너지학회:학술대회논문집
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• 2012.03a
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• pp.457-462
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• 2012

Daylighting software is an important component to predict the performance of daylighting system in advance of a field demonstration study with installing them in buildings. PHOTOPIA is a powerful software to generate a candela distribution curve(CDC) of an active daylighting system like a tracking dish concentrator. With PHOTOPIA, a set of candela distribution curves was generated under clear sky conditions and different solar altitude angles. The candela distribution curves were then imported to RADIANCE for rendering and analysis on the daylighting performance of a tracking dish concentrator when it installed in a actual class room without windows. As a result, the daylight collection efficiency of the dish concentrator was 68.4% when we assumed that there was no tracking error. It was found that candela(cd) and total lumens(lm) increased with solar altitude rising, whereas the distribution angle was fixed. The illuminance uniformity on the work plane in the class room was relatively low, 0.12, while the illuminance uniformity on the area of $2.7m^2$ to which the light was illuminated was considerably high, 0.60. The maximum illuminance was 1,340lux with a solar altitude angle of 80 degrees.

• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.1
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• pp.98-112
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• 1996

Tho purpose of this study was to investigate hygienic and comfortable properties of socks. Materials are nine summer socks either frequently being worn or new products recently introduced to market. Three female and three male adults participated in this study. Through wearing experiment, the numbers of microbes on foot and sock were counted and subjective sensation was measured. The microbes were isolated and identified based on growth physiological characteristics. Nine different socks had smaller number of bacteria of sock than that of foot. The number of bacteria of sock was significantly related with that of foot in cotton socks, in piled cotton socks, in mesh cotton socks, in cotton+ nylon+ linen blended socks, in nylon socks. Total number of bacteria of tv cut finished socks was most small and total number of bacteria was increased in the order of ultra fresh finished socks, untreated cotton socks, nylon socks, cotton + nylon+ linen blended socks, mesh cotton socks, polyester+ nylcn+ linen blended socks, piled cotton socks, cotton socks. Total number of bacteria of cotton socks and piled cotton socks were significantly different from that of uv cut finished socks. Finished socks and .jocks has high air permeability had significantly small number of bacteria. Comfortable sensation in nylon socks and polyester+nylon+linen socks was significantly uncomfortable. The way socks finished and air permeability of .jocks affected theirs hygienic property, while fiber type of them affected comfortablene, is. Bacteria identified were Staphylo coccus aureus, S. au rice larir, S. cahn ii, S. ep ids midis, S. haemo Iyticus, S. h am in 2's. S.fapraphyticus, S. warnery, 1 cinetobater calcoaceticus bio. anitratus, p.reudomonas mendocina, p. paucimobilis, Flavimonas Q ryzihabitans (CDC Group VE-2), and Xanthomanas maltophina. Fungi isolated were Spicaria sp., Thrichoderma sp., Fusarium sp., Aspergillus sp., Epicoccum sp., Cladosporium sp., and Penicillium sp..

• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.2
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• pp.487-496
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• 2018

The person who knows inaccurately about AIDS is less favorable to AIDS infectee, and the low level of knowledge as to AIDS and negative attitude cause bias and stigma of AIDS infectee to act as a social exclusion mechanism to impede early discovery or early treatment of the disease. As the level of knowledge as to AIDS affect the attitude of AIDS, so this study was tried to make use of basic data to develop education program that may help AIDS infectee and prevention of infection who visit dental clinics in the future by grasping the level of knowledge of the students of the dept. of dental hygiene as to AIDS and the attitude for the infectee. The collected the data subject to 360 students. The investigated data used statistic program SPSS 22.0 (SPSS Inc, Chicago, IL, USA) to calculate general characteristics by frequency and percentage, and for the attitude towards AIDS using Social Networking Services surveysinfectees and testing hypothesis of Duncan, Scheffe to see which groups show the difference in the practice level of infection management.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.4
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• pp.1343-1348
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• 2015

Human UHRF1 (ubiquitin-like PHD and RING finger domain-containing 1) has been reported to be over-expressed in many cancers, but its role in ovarian cancer remains elusive. Here, we determined whether knockdown of UHRF1 by lentivirus-mediated shRNA could inhibit ovarian cancer cell growth. Lentivirus-mediated short hairpin RNAs (lv-shRNAs-UHRF1) were designed to trigger the gene silencing RNA interference (RNAi) pathway. The efficiency of lentivirus-mediated shRNA infection into HO-8910 and HO-8910 PM cells was determined using fluorescence microscopy to observe lentivirus-mediated GFP expression and was confirmed to be over 80 percent. UHRF1 expression in infected HO-8910 and HO-8910 PM was evaluated by real-time PCR and Western blot analysis. The Cell Counting Kit-8 (CCK-8) assay was used to measure cell viability; flow cytometry and Hoechst 33342 assay was applied to measure cell cycle arrest and apoptosis. Cell invasion was assessed using transwell chambers. Our results demonstrated that the loss of UHRF1 promoted HO-8910 and HO-8910 PM cell apoptosis, while inhibiting cell proliferation. In addition, UHRF1 knockdown significantly inhibited the invasion of human ovarian cancer cells. In the present study, we also showed that depleting HO-8910 cells of UHRF1 caused activation of the DNA damage response pathway, with the cell cycle arrested in G2/M-phase. The DNA damage response in cells depleted of UHRF1 was illustrated by phosphorylation of CHK (checkpoint kinase) 2 on Thr68, phosphorylation of CDC25 (cell division control 25) on Ser 216 and phosphorylation of CDK1 (cyclin-dependent kinase 1) on Tyr 15.

• The Journal of Korean Medicine
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• v.32 no.4
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• pp.83-99
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• 2011

Objective: This study aimed to evaluate the protective effects of Socheongryong-tang (SCRT) on elastase-induced lung injury. Materials and Methods: The extract of SCRT was treated to A549 cells and elastase-induced COPD mice model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results: SCRT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. SCRT treatment also revealed the protective effect on elastase-induced COPD mice model. This effect was evidenced via histopathological findings including immunofluoresence stains against elastin, collagen, and caspase 3, and protein level of Cdc2, cyclin B1, and phospho-Erk1/2 in lung tissue. Conclusion: These data suggest that SCRT has pharmaceutical properties on COPD. This study provides scientific evidence for the efficacy of SCRT for clinical application to patients with COPD.

• The Journal of Korean Medicine
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• v.32 no.2
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• pp.63-78
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• 2011

Objective: This study aimed to evaluate the protective effects of Maekmundong-tang (MMDT) on an elastase-induced COPD model. Materials and Methods: The extract of MMDT was treated to A549 cells and elastase-induced COPD mice model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results: MMDT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of Cdk1, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. MMDT treatment also revealed a protective effect on the elastase-induced COPD mice model. This effect was evidenced via histopathological finding including immunofluorescence stains against elastin, collagen, and caspase 3, and protein level of Cdc2 in lung tissue. Conclusion: These data suggest that MMDT has pharmaceutical properties on COPD. This study can provide scientific evidence for the efficacy of MMDT for clinical application to patients with COPD.

• Journal of Medicine and Life Science
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• v.16 no.3
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• pp.84-89
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• 2019

Docosahexaenoic acid (DHA), a principal of mackerel-derived fermented fish oil, increases the proliferation of dermal papilla cells (DPCs) via the upregulation of cell cycle-associated proteins such as cyclin D1 and cdc2 p34, and might promote hair-growth. However, the intracellular mechanisms that underlie the action of DHA in the proliferation of DPCs have not been investigated fully. In this study, we addressed the action mechanisms of DHA to trigger the activation of anagen in DPCs. DHA activated β-catenin signaling by the increased phosphorylation at serine 552 and serine 675 as well as the translocation and accumulation of activated β-catenin into the nucleus. In the other hand, DHA inhibited canonical TGF-β/Smad signaling by the decreased phosphorylation of Smad2/3. Taken together, the results indicate that DHA might stimulate anagen signaling via the activation of Wnt/β-catenin pathway, while the inactivation of canonical TGF-β signaling pathway in DPCs.

• International Journal of Oral Biology
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• v.36 no.3
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• pp.129-134
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• 2011

Eugenol is an essential oil found in cloves and cinnamon that is used widely in perfumes. However, the significant anesthetic and sedative effects of this compound have led to its use also in dental procedures. Recently, it was reported that eugenol induces apoptosis in several cancer cell types but the mechanism underlying this effect has remained unknown. In our current study, we examined whether the cytotoxic effects of eugenol upon human melanoma G361 cells are associated with cell cycle arrest and apoptosis using a range of methods including an XTT assay, Hoechst staining, immunocyto-chemistry, western blotting and flow cytometry. Eugenol treatment was found to decrease the viability of the G361 cells in both a time- and dose-dependent manner. The induction of apoptosis in eugenol-treated G361 cells was confirmed by the appearance of nuclear condensation, the release of both cytochrome c and AIF into the cytosol, the cleavage of PARP and DFF45, and the downregulation of procaspase-3 and -9. With regard to cell cycle arrest, a time-dependent decrease in cyclin A, cyclin D3, cyclin E, cdk2, cdk4, and cdc2 expression was observed in the cells after eugenol treatment. Flow cytometry using a FACScan further demonstrated that eugenol induces a cell cycle arrest at S phase. Our results thus suggest that the inhibition of G361 cell proliferation by eugenol is the result of an apoptotic response and an S phase arrest that is linked to the decreased expression of key cell cycle-related molecules.

• Biomedical Science Letters
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• v.22 no.3
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• pp.83-97
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• 2016

The measurement of viral load in HIV-1 infected patients is essential for the establishment of a therapeutic strategy. Several commercial assays have shown shortcomings in quantifying rare genotypes of HIV-1 such as minor groups of N and O. In this study, the HIV-1 RT-qPCR assay was developed. The primers and probe of HIV-1 were designed to target the pol gene and to increase the detection efficiency of various subtypes including group N and O. The HIV-1 quantitative RT-qPCR assay was assessed for its analytical performance and clinical evaluation. The LoD was determined to 33.9 IU/ml. The LoD of several subtypes including A, C, D, CRF_01AE, F, CRF_02AG, G and H, were determined to less than 40 IU/ml. The HIV-1 quantitative RT-qPCR assay was evaluated using the China National Reference Panel of HIV-1 RNA to determine the analytical performance. The results were all within the acceptable range. The clinical evaluation was performed at Hunan CDC in China. The clinical evaluation results were compared with those of the China domestic commercial kit. A significant correlation (fresh samples; $R^2=0.84$, P<0.001, frozen samples; $R^2=0.76$, P<0.001) between the two systems was observed for 64 fresh samples and 76 frozen samples with viral loads, and the Bland-Altman plot showed good agreement (98.4%, 96.1%, respectively). In conclusion, the HIV-1 quantitative RT-qPCR assay had comparable analytical performance with several commercial kits. The study provides basic data for the research of HIV-1 diagnosis and the development of P < HIV-1 molecular diagnostic assay.