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Protective Effects of Maekmundong-tang on Elastase-induced Lung Injury  

Kim, Hyoung-Won (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University)
Yang, Su-Young (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University)
Kim, Min-Hee (Dept. of Neurophysiology, College of Oriental Medicine, Daejeon University)
NamGung, Uk (Dept. of Neurophysiology, College of Oriental Medicine, Daejeon University)
Park, Yang-Chun (Division of Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Daejeon University)
Publication Information
The Journal of Korean Medicine / v.32, no.2, 2011 , pp. 63-78 More about this Journal
Abstract
Objective: This study aimed to evaluate the protective effects of Maekmundong-tang (MMDT) on an elastase-induced COPD model. Materials and Methods: The extract of MMDT was treated to A549 cells and elastase-induced COPD mice model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results: MMDT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of Cdk1, and gene expression of TNF-${\alpha}$ and IL-$1{\beta}$ in A549 cells. MMDT treatment also revealed a protective effect on the elastase-induced COPD mice model. This effect was evidenced via histopathological finding including immunofluorescence stains against elastin, collagen, and caspase 3, and protein level of Cdc2 in lung tissue. Conclusion: These data suggest that MMDT has pharmaceutical properties on COPD. This study can provide scientific evidence for the efficacy of MMDT for clinical application to patients with COPD.
Keywords
Maekmundong-tang (Maimendong-tang); chronic obstructive pulmonary disease; A549 cell; elastase;
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Times Cited By KSCI : 5  (Citation Analysis)
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