• Journal of fish pathology
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• v.37 no.1
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• pp.1-8
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• 2024

The advantages of replicon vectors of RNA viruses include a high ability to stimulate innate immunity and exponential amplification of target mRNA leading to high expression of foreign antigens. The present study aimed to construct a DNA-layered nervous necrosis virus (NNV) replicon vector system in which the capsid protein gene was replaced with a foreign antigen gene and to compare the efficiency of foreign antigen expression between the conventional DNA vaccine vector and the present replicon vector. We presented the first report of a nodavirus DNA replicon-based foreign antigen expression system. Instead of a two-vector system, we devised a one-vector system containing both an NNV RNA-dependent RNA polymerase cassette and a foreign antigen-expressing cassette. This single-vector approach circumvents the issue of low foreign protein expression associated with the low co-transfection efficiency of a two-vector system. Cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 (with the capsid gene ORF replaced with VHSV glycoprotein ORF) exhibited significantly higher transcription of the VHSV glycoprotein gene compared to cells transfected with either a vector without hammerhead ribozyme or a conventional DNA vaccine vector expressing the VHSV glycoprotein. Furthermore, the transcription level of the VHSV glycoprotein in cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 showed a significant increase over time. These results suggest that NNV genome-based DNA replicon vectors have the potential to induce stronger and longer expression of target antigens compared to conventional DNA vaccine vectors.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.6831-6839
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• 2015

Multidrug resistance is a principal mechanism by which tumors become resistant to structurally and functionally unrelated anticancer drugs. Resistance to chemotherapy has been correlated with overexpression of p-glycoprotein (p-gp), a member of the ATP-binding cassette (ABC) superfamily of membrane transporters. P-gp mediates resistance to a broad-spectrum of anticancer drugs including doxorubicin, taxol, and vinca alkaloids by actively expelling the drugs from cells. Use of specific inhibitors/blocker of p-gp in combination with clinically important anticancer drugs has emerged as a new paradigm for overcoming multidrug resistance. The aim of this paper is to review p-gp regulation by dietary nutraceuticals and to correlate this dietary nutraceutical induced-modulation of p-gp with activity of anticancer drugs.

• Microbiology and Biotechnology Letters
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• v.48 no.1
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• pp.32-37
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• 2020

We improved on a unified saccharification and fermentation (USF) system for the direct production of ethanol from agarose by increasing total agarase activity. The pGMFα-NGH plasmid harboring the NABH558 gene encoding neoagarobiose hydrolase and the AGAG1 and AGAH71 genes encoding β-agarase was constructed and used to transform Saccharomyces cerevisiae 2805. NABH558 gene transcription level was increased and total agarase activity was increased by 25 to 40% by placing the NABH558 gene expression cassette upstream of the other gene expression cassettes. In the 2805/pGMFα-NGH transformant, three secretory agarases were produced that efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexaose. During the united cultivation process, a maximum of 2.36 g/l ethanol from 10 g/l agarose was produced over 120 h.

• International Journal of Air-Conditioning and Refrigeration
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• v.15 no.3
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• pp.101-107
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• 2007

Indoor air quality becomes of a concern recently in view of human health. This study investigates the air diffusion performance and the air change efficiency of a classroom, when outdoor air is introduced in two different ways in addition to the heating/cooling operation of a ceiling-mounted heat pump. A CFD analysis has been performed to investigate the effect of the discharge angle of the air jets from the heat pump for both parallel and series types of outdoor air system. It is observed that the series type creates more uniform indoor environment compared to the parallel type in general. It can be concluded the discharge angle should not be larger than 40o for the parallel type, in order not to generate thermal stratification in the room.

• Proceedings of the IEEK Conference
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• 1998.10a
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• pp.293-296
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• 1998

When the information of announcement facility is modified or added, they are recorded on a tape and then are updated manually using the cassette tape recorder at each office. But the degrade of the tone quality owing to repeated use of the tape and some recording mistakes by processing manually deteriorate the quality of service. And new services such as IN(Intelligent Network) and added functions make the announcement changed very often. Therefore the improved skill of the announcement handling is needed. This paper introduces the implementation of the TDX-10A on-line recording function of transferring voice in PCM data file and storing it in the memory of the recorded announcement facility, when message is recorded onthe fixed type of announcement facility.

• Journal of Advanced Marine Engineering and Technology
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• v.29 no.5
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• pp.540-551
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• 2005

In this research, We'll focus on warm environment and ventilation characteristics when utilizing intake ports, exhaust port and 4-way cassettes for removing heat and polluted air. Four way cassettes have been typical air conditioning units for offices since the multi air-conditioner was introduced. The following installation cases will also be discussed: 1) 4-way cassette's operation without intake ports and exhaust port. 2) Effect of the position of intake port and exhaust ports. 3) cooling air flow, temperature. distribution and local supply index according to the air flow rate from 4-way cassettes.

• Journal of Biomedical Engineering Research
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• v.16 no.2
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• pp.139-148
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• 1995

In this paper we report the device of a multi-channel clinical instrument developed for determi¬nation of the levels of the urinary urobilinogen, glucose, ketone, bilirubin, protein, ascorbic acid, nitrite, pH, occult-blood, specific gravity, and leukocytes semiquantitatively. The test parameters are expressed on the dry test strips as a range of color intensities by chemical reactions. The instrument measures the value of each substance by reading the reflectance light emanated from the test strips. We also designed the reagent strip cassette and loader in order to intercept the outside interference. The loader can be operated semi-automatically. The light source is consisted on light emitting diodes at three specific wavelengths (560 nm, 610 nm, 650 nm). Precision of the system was evaluated by testing a series of commercial control urine samples. Furthermore, the performance of the instrument was compared with two other test methods on the urine samples from 100 persons. Our results showed a good repeatability between tests and a satisfactory agreement between the readings by our instrument and visual evaluation.

• Journal of Broadcast Engineering
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• v.1 no.2
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• pp.142-151
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• 1996

The digital video cassette recorder(DVCR) is considered as next generation VCR due to its performance breakthrough in various aspects, such as digital recording and various digital image processing techniques. The purpose of our study is to understand the standardized specifications of the DVCR system, evaluate the performance of the system, and improve the quality of the reconstructed DVCR image. More specifically, in order to enhance the DVCR Image we consider a series of discrete cosine transform(DCT), quantization, inverse DCT, and Inverse quantization as a degradation process of the Imaging system, and propose a fast adaptive image restoration algorithm for reducing blocking artifacts.

• Journal of Microbiology and Biotechnology
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• v.11 no.1
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• pp.61-64
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• 2001

Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double $\delta$ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single $\delta$ system.

• Journal of the Korean Society for Precision Engineering
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• v.31 no.8
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• pp.697-703
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• 2014

This paper is study of solving vibration problem occurred in moving hand of wafer transfer robot in semiconductor manufacturing line. Long settling time for decreasing vibration makes low production rate, and moreover the excessive vibration of hand sometimes breaks the wafer in a cassette. The ways of reducing the moving speed and changing the type of motion profile did not help for lessening vibration. Therefore, we analyzed the mechanical property of the hand such as natural frequency, and frequency component of the motion profile currently used in the manufacturing line. In several conditions of motion profile, we found the best condition of which the frequency component in near of natural frequency of the hand is minimal and this induced small vibration in moving hand. The results were verified theoretically and experimentally using frequency analysis.