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http://dx.doi.org/10.4014/mbl.1909.09008

Improvement of a Unified Saccharification and Fermentation System for Agaro-bioethanol Production in Yeast  

Lee, So-Eun (Biomedical Engineering and Biotechnology, Division of Applied Bioengineering, College of Engineering, Dong-Eui University)
Kim, Yeon-Hee (Biomedical Engineering and Biotechnology, Division of Applied Bioengineering, College of Engineering, Dong-Eui University)
Publication Information
Microbiology and Biotechnology Letters / v.48, no.1, 2020 , pp. 32-37 More about this Journal
Abstract
We improved on a unified saccharification and fermentation (USF) system for the direct production of ethanol from agarose by increasing total agarase activity. The pGMFα-NGH plasmid harboring the NABH558 gene encoding neoagarobiose hydrolase and the AGAG1 and AGAH71 genes encoding β-agarase was constructed and used to transform Saccharomyces cerevisiae 2805. NABH558 gene transcription level was increased and total agarase activity was increased by 25 to 40% by placing the NABH558 gene expression cassette upstream of the other gene expression cassettes. In the 2805/pGMFα-NGH transformant, three secretory agarases were produced that efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexaose. During the united cultivation process, a maximum of 2.36 g/l ethanol from 10 g/l agarose was produced over 120 h.
Keywords
Unified enzymatic saccharification and fermentation (USF) system; ${\beta}$-agarase; neoagarobiose hydrolase; bioethanol; recombinant yeast;
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Times Cited By KSCI : 2  (Citation Analysis)
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