Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Improvement of a Unified Saccharification and Fermentation System for Agaro-bioethanol Production in Yeast

  • Lee, So-Eun (Biomedical Engineering and Biotechnology, Division of Applied Bioengineering, College of Engineering, Dong-Eui University) ;
  • Kim, Yeon-Hee (Biomedical Engineering and Biotechnology, Division of Applied Bioengineering, College of Engineering, Dong-Eui University)
  • Received : 2019.09.17
  • Accepted : 2019.10.29
  • Published : 2020.03.28
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Abstract

We improved on a unified saccharification and fermentation (USF) system for the direct production of ethanol from agarose by increasing total agarase activity. The pGMFα-NGH plasmid harboring the NABH558 gene encoding neoagarobiose hydrolase and the AGAG1 and AGAH71 genes encoding β-agarase was constructed and used to transform Saccharomyces cerevisiae 2805. NABH558 gene transcription level was increased and total agarase activity was increased by 25 to 40% by placing the NABH558 gene expression cassette upstream of the other gene expression cassettes. In the 2805/pGMFα-NGH transformant, three secretory agarases were produced that efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexaose. During the united cultivation process, a maximum of 2.36 g/l ethanol from 10 g/l agarose was produced over 120 h.

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References

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