• 한국어병학회지
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• 제37권1호
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• pp.1-8
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• 2024

The advantages of replicon vectors of RNA viruses include a high ability to stimulate innate immunity and exponential amplification of target mRNA leading to high expression of foreign antigens. The present study aimed to construct a DNA-layered nervous necrosis virus (NNV) replicon vector system in which the capsid protein gene was replaced with a foreign antigen gene and to compare the efficiency of foreign antigen expression between the conventional DNA vaccine vector and the present replicon vector. We presented the first report of a nodavirus DNA replicon-based foreign antigen expression system. Instead of a two-vector system, we devised a one-vector system containing both an NNV RNA-dependent RNA polymerase cassette and a foreign antigen-expressing cassette. This single-vector approach circumvents the issue of low foreign protein expression associated with the low co-transfection efficiency of a two-vector system. Cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 (with the capsid gene ORF replaced with VHSV glycoprotein ORF) exhibited significantly higher transcription of the VHSV glycoprotein gene compared to cells transfected with either a vector without hammerhead ribozyme or a conventional DNA vaccine vector expressing the VHSV glycoprotein. Furthermore, the transcription level of the VHSV glycoprotein in cells transfected with a vector harboring hammerhead ribozyme-fused RNA1 and RNA2 showed a significant increase over time. These results suggest that NNV genome-based DNA replicon vectors have the potential to induce stronger and longer expression of target antigens compared to conventional DNA vaccine vectors.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.6831-6839
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• 2015

Multidrug resistance is a principal mechanism by which tumors become resistant to structurally and functionally unrelated anticancer drugs. Resistance to chemotherapy has been correlated with overexpression of p-glycoprotein (p-gp), a member of the ATP-binding cassette (ABC) superfamily of membrane transporters. P-gp mediates resistance to a broad-spectrum of anticancer drugs including doxorubicin, taxol, and vinca alkaloids by actively expelling the drugs from cells. Use of specific inhibitors/blocker of p-gp in combination with clinically important anticancer drugs has emerged as a new paradigm for overcoming multidrug resistance. The aim of this paper is to review p-gp regulation by dietary nutraceuticals and to correlate this dietary nutraceutical induced-modulation of p-gp with activity of anticancer drugs.

• 한국미생물·생명공학회지
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• 제48권1호
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• pp.32-37
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• 2020

We improved on a unified saccharification and fermentation (USF) system for the direct production of ethanol from agarose by increasing total agarase activity. The pGMFα-NGH plasmid harboring the NABH558 gene encoding neoagarobiose hydrolase and the AGAG1 and AGAH71 genes encoding β-agarase was constructed and used to transform Saccharomyces cerevisiae 2805. NABH558 gene transcription level was increased and total agarase activity was increased by 25 to 40% by placing the NABH558 gene expression cassette upstream of the other gene expression cassettes. In the 2805/pGMFα-NGH transformant, three secretory agarases were produced that efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexaose. During the united cultivation process, a maximum of 2.36 g/l ethanol from 10 g/l agarose was produced over 120 h.

• International Journal of Air-Conditioning and Refrigeration
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• 제15권3호
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• pp.101-107
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• 2007

Indoor air quality becomes of a concern recently in view of human health. This study investigates the air diffusion performance and the air change efficiency of a classroom, when outdoor air is introduced in two different ways in addition to the heating/cooling operation of a ceiling-mounted heat pump. A CFD analysis has been performed to investigate the effect of the discharge angle of the air jets from the heat pump for both parallel and series types of outdoor air system. It is observed that the series type creates more uniform indoor environment compared to the parallel type in general. It can be concluded the discharge angle should not be larger than 40o for the parallel type, in order not to generate thermal stratification in the room.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 1998년도 추계종합학술대회 논문집
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• pp.293-296
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• 1998

When the information of announcement facility is modified or added, they are recorded on a tape and then are updated manually using the cassette tape recorder at each office. But the degrade of the tone quality owing to repeated use of the tape and some recording mistakes by processing manually deteriorate the quality of service. And new services such as IN(Intelligent Network) and added functions make the announcement changed very often. Therefore the improved skill of the announcement handling is needed. This paper introduces the implementation of the TDX-10A on-line recording function of transferring voice in PCM data file and storing it in the memory of the recorded announcement facility, when message is recorded onthe fixed type of announcement facility.

• Journal of Advanced Marine Engineering and Technology
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• 제29권5호
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• pp.540-551
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• 2005

In this research, We'll focus on warm environment and ventilation characteristics when utilizing intake ports, exhaust port and 4-way cassettes for removing heat and polluted air. Four way cassettes have been typical air conditioning units for offices since the multi air-conditioner was introduced. The following installation cases will also be discussed: 1) 4-way cassette's operation without intake ports and exhaust port. 2) Effect of the position of intake port and exhaust ports. 3) cooling air flow, temperature. distribution and local supply index according to the air flow rate from 4-way cassettes.

• 대한의용생체공학회:의공학회지
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• 제16권2호
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• pp.139-148
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• 1995

In this paper we report the device of a multi-channel clinical instrument developed for determi¬nation of the levels of the urinary urobilinogen, glucose, ketone, bilirubin, protein, ascorbic acid, nitrite, pH, occult-blood, specific gravity, and leukocytes semiquantitatively. The test parameters are expressed on the dry test strips as a range of color intensities by chemical reactions. The instrument measures the value of each substance by reading the reflectance light emanated from the test strips. We also designed the reagent strip cassette and loader in order to intercept the outside interference. The loader can be operated semi-automatically. The light source is consisted on light emitting diodes at three specific wavelengths (560 nm, 610 nm, 650 nm). Precision of the system was evaluated by testing a series of commercial control urine samples. Furthermore, the performance of the instrument was compared with two other test methods on the urine samples from 100 persons. Our results showed a good repeatability between tests and a satisfactory agreement between the readings by our instrument and visual evaluation.

• 방송공학회논문지
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• 제1권2호
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• pp.142-151
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• 1996

디지털 비디오 카세트 레코더(DVCR)는 디지털 기록과 다양한 디지털 영상처리 기술과 같은 여러가지 측면에서 놀랄만한 성능에 힘입어 차세대 VCR로 대두되고 있다. 본 논문에서는 DVCR 시스템의 표준규격을 이해하고, 성능을 평가하고, 재생된 DVCR의 영상을 개선시키는 알고리듬을 제안하고자 한다. 보다 자세히 설명하자면, DVCR 영상을 개선하기 위하여 이산 코사인 변환, 양자화, 역 이산코사인 변환과 역양자화를 영상 시스템의 열화요인으로 모델링하고, 이러한 열화모델 때문에 발생하는 블럭 현상을 적응적으로 복원하는 고속 알고리듬을 제안한다.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.61-64
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• 2001

Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double $\delta$ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single $\delta$ system.

• 한국정밀공학회지
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• 제31권8호
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• pp.697-703
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• 2014

This paper is study of solving vibration problem occurred in moving hand of wafer transfer robot in semiconductor manufacturing line. Long settling time for decreasing vibration makes low production rate, and moreover the excessive vibration of hand sometimes breaks the wafer in a cassette. The ways of reducing the moving speed and changing the type of motion profile did not help for lessening vibration. Therefore, we analyzed the mechanical property of the hand such as natural frequency, and frequency component of the motion profile currently used in the manufacturing line. In several conditions of motion profile, we found the best condition of which the frequency component in near of natural frequency of the hand is minimal and this induced small vibration in moving hand. The results were verified theoretically and experimentally using frequency analysis.