• Natural Product Sciences
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• v.13 no.2
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• pp.118-122
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• 2007

We have studied the cytoprotective effect on H$_2$O$_2$ induced oxidative stress from leaves of Carpinus tschonoskii. The methanol extract of Carpinus tschonoskii was found to scavenge intracellular reactive oxygen species (ROS) using flow cytometry and confocal microscope. This extract prevented lipid peroxidation and thus reduced cell death of Chinese hamster lung fibroblast (V79-4) induced by H$_2$O$_2$ treatment. The extract increased catalase activity and phosphorylation of extracellular signal regulated kinase (ERK). Taken together, the results suggest that Carpinus tschonoskii protects V79-4 cells against oxidative damage by H$_2$O$_2$ through scavenging ROS.

• The Korean Journal of Ecology
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• v.21 no.6
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• pp.809-814
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• 1998

Changes of annual seed production related to climate change were studied for 12 years in Piagol, a riparian valley in Mt. Jiri. Sixty-four seed traps (sized 0.5 ${\times}$ 0.5 $m^{2}$) were set up on the forest floor of surveyed area. Seeds were collected from these traps at an interval of 15 days from September to November since 1984. Vegetation of the study area was mainly consisted of the naturally regenerated Carpinus tschonoskii in the tree layer. Acer mono, Quercus serrata, Carpinus laxiflora and Symplocos chinensis also appeared in the same layer. Maximum production occurred in 1984 and 1994. As a result of comparing seed production with local climate factors for 12 years, seed productivity and the year of maximum production of Carpinus forest were merely related with precipitation, air temperature and duration of sunshine among local climate factors. Duration of sunshine was, however, not contributed to periodically high productivity of seed of riparian valley carpinus forest.

• The Korean Journal of Ecology
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• v.11 no.3
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• pp.109-122
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• 1988

The foret vegetations of Daedunsan provincial park area in Korea were classified into eight communities of Acer mono-Zelkova serrata, Lindera erythrocarpa-Cornus controversa, Carpinus tschonoskii, Quercus variabilis, Quercus serrata, Carpinus laxiflora, Rhododendron schlippenbachii-Quercus mongolica and Rhododendron mucronu-latum-Pinus densiflora by the Z-M method. By two dimensional analysis of temperature, moisture gradients, the eight communities were grouped into four vegetation types: cove forest dominated with Zelkova serrata and Cornus controversa, hornbeam forest with Carpinus tschonoskii and Carpinus laxiflora, oak forest with Quercus variabilis, Quercus mongolica, Carpinus laxiflora, Carpinus tschonoskii, Zelkova serrta and Pinus densiflora community was made from the analysis of actual vegetation map by the phytosociological classification, environmental conditions and human interferences.

• The Korean Journal of Ecology
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• v.11 no.3
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• pp.131-136
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• 1988

The continuum analyses and polar ordinations were applied for the ordination of forest vegetation in Mt. Naejang, national park in Korea. In the continuum analyses the sequence of Pinus densiflora, Quercus mongolica, Quercus variabilis, Carpinus laxiflora, Carpinus tschonoskii, Lindera erythrocapa and Zelkova serrata community was arranged along the continuum gradient as in moisture gradient analyses. The positive correlation of r=0.83 between continuum index and soil moisture content was observed. In the polar ordinations ten communities of Pinus densiflora, Quercus monogolica, Quercus variabilis, Carpinus laxiflora, Daphniphyllum macropodum, carpinus tschonoskii, Quercus aliena-Carpinus tschonoskii, Torreya nucifera, Cornus controversa-Lindera erythrocarpa and Zelkova serrata were identified. However, continuous distribution pattern of ten communities mentioned above could be regarded as a vegetational continuum. The results of these ordinations also were corresponded to those of phytosociological classification.

• The Korean Journal of Ecology
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• v.10 no.4
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• pp.159-164
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• 1987

The potential natural vegetation of Seonunsan area, southwestern Korea, was inferred from the actual vegetation. In previous two papers the plant communities of actual vegetation of the area is grouped into nine types; Quercus variabilis, Pinus densiflora, Carpinus tschonoskii, Quercus serrata, Camellia japonica (plantation), Quercus aliena, Pinus thunbergii, Zelkova serrata and Carpinus laxiflora forest. With the analysis of species richness, age structure and various informations on vegetation changes of the plant communities, two paths of late stage succession are suggested in climatic climax starting from Pinus densiflora forest in the area. One is through Quercus variabilis forest to Carpinus laxiflora forest in upper parts of the mountain and the other through Quercus aliena forest to Carpinus tschonoskii forest in lower parts of the mountain. With analysis of actual vegetation and the examination of informations including human activities in the area, the potential natural vegetation of the area was inferred. The potential natural vegetation of the area was mainly composed of Carpinus laxiflora, Carpinus tschonoskii, Pinus densiflora and Zelkova serrata forest. The actual vegetation map and potential natural vegetation map (scale, 1:25, 000) and other results from this study might be the useful data for the protection of natural vegetation and restoration of the current vegetation.

• Toxicological Research
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• v.28 no.4
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• pp.255-262
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• 2012

Inflammation is the immune system's response to infection and injury-related disorders, and is related to pro-inflammatory factors (NO, $PGE_2$, cytokines, etc.) produced by inflammatory cells. Atopic dermatitis (AD) is a representative inflammatory skin disease that is characterized by increasing serum levels of inflammatory chemokines, including macrophage-derived chemokine (MDC). Carpinus tschonoskii is a member of the genus Carpinus. We investigated the anti-inflammatory activity of C. tschonoskii by studying the effects of various solvent fractions prepared from its leaves on inflammatory mediators in HaCaT and RAW264.7 cells. We found that the chloroform fraction of C. tschonoskii inhibited MDC at both the protein and mRNA levels in HaCaT cells, acting via the inhibition of STAT1 in the IFN-${\gamma}$ signaling pathway. In addition, the chloroform fraction significantly suppressed the expression of inflammatory factors induced by lipopolysaccharide stimulation, except COX-2 and TNF-${\alpha}$. These results suggest that the chloroform fraction of C. tschonoskii leaves may include a component with potential anti-inflammatory activity.

• Biomolecules & Therapeutics
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• v.18 no.4
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• pp.454-462
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• 2010

The present study investigated the neuroprotective effect of Carpinus tschonoskii MAX and its intracellular protective mechanism on 6-hydroxydopamine (6-OHDA)-induced oxidative damage in PC12 cells. We found that pretreatment of PC12 cells with C. tschonoskii extract significantly inhibited the cell death induced by 6-OHDA in a dose dependent manner. C. tschonoskii extract decreased 6-OHDA-induced apoptotic events such as chromatin condensation, DNA fragmentation, the decrease of Bcl-2/Bax ratio, caspase-3 activation and PARP cleavage. C. tschonoskii extract also reduced generation of 6-OHDA-induced reactive oxygen species and nitric oxide. Furthermore, C. tschonoskii extract up-regulated the myocyte enhancer factor 2 D (MEF2D), a critical transcription factor for neuronal survival, and Akt activity, whereas it inhibited the activity of ERK1/2 and JNK. The results suggest that C. tschonoskii extract decreases 6-OHDA-induced oxidative stress and could prevent PC12 cell apoptosis induced by 6-OHDA via the up-regulation of MEF2D and Akt activity, and thus may have application in developing therapeutic agents for Parkinson's disease.

• The Korean Journal of Ecology
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• v.11 no.3
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• pp.145-152
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• 1988

The potential natural vegetation of Naejangsan national park area, southwestern Korea, was inferred from the actual vegetation. With the phytosociological classification, ordinatins and field surveys, the actual vegetation map of the area was made in scale 1:25, 000, including ten communities of Pinus densiflora, quercus mongolica, Quercus variabilis, Carpinus laxiflora, Daphnipyllum macropodum, Carpinus tschonoskii, Quercus aliena-Carpinus tschonoskii, Cornus controversa-Lindera erythrocarpa, Torreya mucifera-Zelkova serrate and Acer mono-Zelkova serrata community. The analyses of species richness, age structure and various informations on vegetation changes suggest the three pathways of late stage succession from P. densiflora forest to climatic climax. The first of them is through Q. variabilis forest to Q. monogolica forest in the upper parts of the mountain, the second through Q. variabilis and Q. serrata forest to C. laxiflora forest in the middle parts and the third through Q. aliena forest to C. tschonoskii forest in lower parts. Considering the actual vegetation and informations on the vegetation changes including human activities, the potential natural vegetation of the mountain mainly composed of Q. monogolica, C. laxiflora, C. tschonoskii, P. densiflora and Z. serrata forest as climatic climax and/or edaphic climax was inferred. The present situration of nature conservation in the area was estimated by the examination on the actual vegetation and potential natural vegetation map.

• The Korean Journal of Ecology
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• v.9 no.4
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• pp.209-223
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• 1986

The forest stands of Seonusan area, South-western Korea, were classified into three alliances and nine communities by the Z-M school scheme. Of these one alliance and four association were recognized in this study, that is, an alliance, Carpinion laxiflorae and four associations, Casrpinetum tschonoskii, Quercetum variabilis, Carpinetum laxiflorae and Rhododendro mucronulati-Pinetum densiflorae. Hierachy of Seonunsan area forest vegetation by Z-M scheme was as fallows: Pinion densiflorae Suz.-Tok. 1966. 1. Rhododendro mucronlati-Pinetum densiflorae ass.l nov., 2. Pinus thunbergii community. Carpinion laxiflorae all. nov., 1. Quercus serrata-Carpinus tschonoskii community, 2. Quercus aliena-Carpinus tschonoskii community, 3, Carp inetum tschonoskii ass. nov., a. Typical subass., b. Sasa borealis subass. 4. Quercetum variabilis ass. nov., a Sasa borealis subass. b. Typical subass, 5. Carpinetum laxiflorae ass. nov., Zelkovion serratae Miyawaki et al., 1977., 1. Orixo-Zelk ovetum serratae Miyawaki et H. Tohma 1975., a Typical subass., b. Thea sinensis facies, 2. Thea sinensis-Camellia japonica community.

• Korean Journal of Environment and Ecology
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• v.25 no.4
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• pp.562-572
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• 2011

This study is to examine the environmental characteristics of subpopulations of Ilex crenata var. microphyllla Max. distributed in the east-west slopes of Mt. Halla National Park based on analyzing the factors of environment, vegetation and soil. The result of the TWINSAPN analysis conducted on 56 study plots between the altitudes of 500 m and 1600 m of east-west slopes of Mt. Halla National Park can be divided into five communities-Prunus maximowiczii Rupr., Manshurian fullmoon Maple, Carpinus tschonoskii Max., Daphniphyllum macropodum Miq., Quercus serrata Thunb. ex Murray and Pinus thunbergil Parl. Among these communities, the plot with high importance percentage of the subpopulation of Ilex crenata var. microphyllla Max., is the Carpinus tschonoskii Max.-Daphniphyllum macropodum Miq. included in the altitude of 600m~1200m radius. The dominant species of the upper shrub layer was Carpinus tschonoskii Max.(I.P.: 29.82%) while the dominant species of the canopy layer was Daphniphyllum macropodum Miq.(I.P.: 26.76%). For the understory layer the dominant species was Ilex crenata var. microphyllla Max.. Species diversity index was on the low end with 0.7424, indicating the stably formed climax community of Carpinus tschonoskii Max.. The analysis of soil properties showed high concentrations of organic matters, available phosphate and Ca, Mg, the high importance percentage of the of Ilex crenata var. microphyllla Max. is found in altitude of 600m~800m on the eastern slope and the altitude of 800m~1,000m radius on the west slope. This region is included in the southern part of the cold/hot forest zone, its high importance percentage of Carpinus tschonoskii Max. and low in the species diversity index of 0.7424, and has formed stable climax community of Carpinus tschonoskii Max. in Mt. Halla.