Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Neuroprotective Effects of Carpinus tschonoskii MAX on 6-Hydroxydopamine-Induced Death of PC12 Cells

  • Kim, Min-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kim, Sang-Cheol (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kang, Jung-Il (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Boo, Hye-Jin (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Hyun, Jin-Won (Department of Biochemistry, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Koh, Young-Sang (Department of Microbiology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Park, Deok-Bae (Department of Histology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kang, Ji-Hoon (Department of Neurology, School of Medicine, Institute of Medical Sciences, Jeju National University) ;
  • Kang, Hee-Kyoung (Department of Pharmacology, School of Medicine, Institute of Medical Sciences, Jeju National University)
  • Received : 2010.08.27
  • Accepted : 2010.10.14
  • Published : 2010.10.31
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Abstract

The present study investigated the neuroprotective effect of Carpinus tschonoskii MAX and its intracellular protective mechanism on 6-hydroxydopamine (6-OHDA)-induced oxidative damage in PC12 cells. We found that pretreatment of PC12 cells with C. tschonoskii extract significantly inhibited the cell death induced by 6-OHDA in a dose dependent manner. C. tschonoskii extract decreased 6-OHDA-induced apoptotic events such as chromatin condensation, DNA fragmentation, the decrease of Bcl-2/Bax ratio, caspase-3 activation and PARP cleavage. C. tschonoskii extract also reduced generation of 6-OHDA-induced reactive oxygen species and nitric oxide. Furthermore, C. tschonoskii extract up-regulated the myocyte enhancer factor 2 D (MEF2D), a critical transcription factor for neuronal survival, and Akt activity, whereas it inhibited the activity of ERK1/2 and JNK. The results suggest that C. tschonoskii extract decreases 6-OHDA-induced oxidative stress and could prevent PC12 cell apoptosis induced by 6-OHDA via the up-regulation of MEF2D and Akt activity, and thus may have application in developing therapeutic agents for Parkinson's disease.

Keywords

References

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