Cytoprotective Activity of Carpinus tschonoskii against H$_2$O$_2$ Induced Oxidative Stress

  • Zhang, Rui (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Kang, Kyoung-Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Piao, Mei-Jing (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Park, Jae-Woo (Department of Nuclear and Energy Engineering and Applied Radiological Science Research Institute, Cheju National University) ;
  • Shin, Taek-Yun (Department of Veterinary Medicine, Cheju National University) ;
  • Yoo, Byoung-Sam (Cosmetic R&D Center, COSMAX Inc.) ;
  • Yang, Young-Taek (Jeju-do Agricultural Research & Extension Services) ;
  • Hyun, Jin-Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
  • Published : 2007.06.30

Abstract

We have studied the cytoprotective effect on H$_2$O$_2$ induced oxidative stress from leaves of Carpinus tschonoskii. The methanol extract of Carpinus tschonoskii was found to scavenge intracellular reactive oxygen species (ROS) using flow cytometry and confocal microscope. This extract prevented lipid peroxidation and thus reduced cell death of Chinese hamster lung fibroblast (V79-4) induced by H$_2$O$_2$ treatment. The extract increased catalase activity and phosphorylation of extracellular signal regulated kinase (ERK). Taken together, the results suggest that Carpinus tschonoskii protects V79-4 cells against oxidative damage by H$_2$O$_2$ through scavenging ROS.

Keywords

References

  1. Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D., and Mitchell, J.B., Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936- 941 (1987)
  2. Chang, C.S. and Jeon, J.I., Foliar flavonoids of the most primitive group, sect. Distegocarpus within the genus Carpinus. Biochem. Syst. Ecol. 32, 35-44 (2004) https://doi.org/10.1016/S0305-1978(03)00186-8
  3. Cooke, M.S., Mistry, N., Wood, C., Herbert, K.E., and Lunec, J., Immunogenicity of DNA damaged by reactive oxygen species implications for anti-DNA antibodies in lupus. Free Radic. Biol. Med. 22, 151-159 (1997) https://doi.org/10.1016/S0891-5849(96)00283-3
  4. Cui, X.Y., Fu, P.F., Pan, D.N., Zhao, Y., Zhao, J., and Zhao, B.C., The antioxidant effects of ribonuclease inhibitor. Free Radic. Res. 37, 1079-1085 (2003) https://doi.org/10.1080/10715760310001600408
  5. Darley-Usmar, V. and Halliwell, B., Blood radicals: reactive nitrogen species, reactive oxygen species, transition metal ions, and the vascular system. Pharm. Res. 13, 649-662 (1996) https://doi.org/10.1023/A:1016079012214
  6. Doctrow, S.R., Huffman, K., Marcus, C.B., Tocco, G., Malfroy, E., Adinolfi, C.A., Kruk, H., Baker, K., Lazarowych, N., Mascarenhas, J., and Malfroy, B., Salen-manganese complexes as catalytic scavengers of hydrogen peroxide and cytoprotective agents: structure-activity relationship studies. J. Med. Chem. 45, 4549-4558 (2002) https://doi.org/10.1021/jm020207y
  7. Farinati, F., Cardin, R., Degan, P., Rugge, M., Mario, F.D., Bonvicini, P., and Naccarato, R., Oxidative DNA damage accumulation in gastric carcinogenesis. Gut 42, 351-356 (1998) https://doi.org/10.1136/gut.42.3.351
  8. Hachiya, M. and Akashi, M., Catalase regulates cell growth in HL60 human promyelocytic cells: evidence for growth regulation by $H_2O_2$. Radiat. Res. 163, 271-282 (2005) https://doi.org/10.1667/RR3306
  9. Jang, H.H. and Surh, Y.J., Protective effects of resveratrol on b-amyloid induced oxidative PC12 cell death. Free Radic. Biol. Med. 34, 1100- 1110 (2003) https://doi.org/10.1016/S0891-5849(03)00062-5
  10. Kim, H.J., So, Y.J., Jang, J.H., Lee, J.S., Oh, Y.J., and Surh, Y.J., Differential cell death induced by salsolinol with and without copper: possible role of reactive oxygen species. Mol. Pharmacol. 60, 440-449 (2001)
  11. Laurindo, F.R., da Luz, P.L., Uint, L., Rocha, T.F., Jaeger, R.G., and Lopes, E.A. Evidence for superoxide radical-dependent coronary vasospasm after angioplasty in intact dogs. Circulation 83, 1705-1715 (1991) https://doi.org/10.1161/01.CIR.83.5.1705
  12. Lee, P.W., Kim, H.S., and Eom, Y.G., Wood anatomy of Genus Carpinus grown in Korea. Seoul Natl. Univ. J. Agric. Sci. 14, 41-48 (1989)
  13. Misra, H.P. and Fridovich, I., The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247, 3170-3175 (1972)
  14. Nakazono, K., Watanabe, N., Matsuno, K., Sasaki, J., Sato, T., and Inoue, M., Does superoxide underlie the pathogenesis of hypertension? Proc. Natl. Acad. Sci. USA. 88, 10045-10048 (1991)
  15. Ohkawa, H., Ohishi, N., and Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  16. Palinski, W., Miller, E., and Witztum, J.L., Immunization of low density lipoprotein (LDL) receptor-deficient rabbits with homologous malondialdehyde-modified LDL reduces atherogenesis. Proc. Natl. Acad. Sci. USA. 92, 821-825 (1995)
  17. Parthasarathy, S., Steinberg, D., and Witztum, J.L., The role of oxidized low-density lipoproteins in the pathogenesis of atherosclerosis. Annu. Rev. Med. 43, 219-225 (1992) https://doi.org/10.1146/annurev.me.43.020192.001251
  18. Pietarinen, P., Raivio, K., Devlin, R.B., Crapo J.D., Chang L.Y., Kinnula V.L., Catalase and glutathione reductase protection of human alveolar macrophages during oxidant exposure in vitro. Am. J. Respir. Cell. Mol. Biol. 13, 434-441 (1995) https://doi.org/10.1165/ajrcmb.13.4.7546773
  19. Rosenkranz, A.R., Schmaldienst, S., Stuhlmeier, K.M., Chen, W., Knapp, W., and Zlabinger, G.J., A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescein-diacetate. J. Immunol. Meth. 156, 39-45 (1992) https://doi.org/10.1016/0022-1759(92)90008-H
  20. Sun, C., Shan, C.Y., Gao, X.D., and Tan, R.X., Protection of PC12 cells from hydrogen peroxide-induced injury by EPS2, an exopolysaccharide from a marine filamentous fungus Keissleriella sp. YS4108. J. Biotechnol. 115, 137-144 (2005) https://doi.org/10.1016/j.jbiotec.2004.08.011