• Parasites, Hosts and Diseases
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• 제46권1호
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• pp.45-48
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• 2008

To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SOS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.

• 한국광학회지
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• 제6권4호
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• pp.379-384
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• 1995

광대역 종합통신망 구축을 위해서는 저손실 및 넓은 대역폭을 갖는 광섬유의 사용이 필수적이며 또한 가입자까지 선로를 광케이블화 하기 위해서는 다심광케이블이 필요하다. 다심광케이블의 고밀도 실장 및 일괄접속을 만족하는 케이블 구조로는 리본광섬유가 폭을 가지고 있어 리본광케이블의 구부림시 광섬유 각각의 길이차에 의해 손실이 발생할 수 있기 때문에 유니트의 저장 및 제조상 주의가 필요하다. 본 연구에서는 리본광 케이블 유니트의 구부림 반경에 따른 광섬유의 스트레인과 구부림반경을 이론적으로 계산하였고 또한 손실의 원인인 측압을 계산하였다. 그리고 실험적으로 여러 유니트의 구부림반경에 따라 각 광섬유의 손실을 측정한 결과 이론적 분석 결과와 잘 부합됨을 알 수 있었다. 이 결과는 리본광케이블 제작시 리본광케이블 유니트의 보관 및 제조공정중에 사용할 릴의 적정 반경을 결정하는 것의 중요성을 보여준다.

• Restorative Dentistry and Endodontics
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• 제41권4호
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• pp.255-261
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• 2016

Objectives: This study aimed to analyze the mineral composition of naturally- and artificially-produced caries-affected root dentin and to determine the elemental incorporation of resin-modified glass ionomer (RMGI) into the demineralized dentin. Materials and Methods: Box-formed cavities were prepared on buccal and lingual root surfaces of sound human premolars (n = 15). One cavity was exposed to a microbial caries model using a strain of Streptococcus mutans. The other cavity was subjected to a chemical model under pH cycling. Premolars and molars with root surface caries were used as a natural caries model (n = 15). Outer caries lesion was removed using a carbide bur and a hand excavator under a dyeing technique and restored with RMGI (FujiII LC, GC Corp.). The weight percentages of calcium (Ca), phosphate (P), and strontium (Sr) and the widths of demineralized dentin were determined by electron probe microanalysis and compared among the groups using ANOVA and Tukey test (p < 0.05). Results: There was a pattern of demineralization in all models, as visualized with scanning electron microscopy. Artificial models induced greater losses of Ca and P and larger widths of demineralized dentin than did a natural caries model (p < 0.05). Sr was diffused into the demineralized dentin layer from RMGI. Conclusions: Both microbial and chemical caries models produced similar patterns of mineral composition on the caries-affected dentin. However, the artificial lesions had a relatively larger extent of demineralization than did the natural lesions. RMGI was incorporated into the superficial layer of the caries-affected dentin.

• 한국응용과학기술학회지
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• 제35권4호
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• pp.1276-1284
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• 2018

probiotics strains promoting the health are a collection of microorganisms that improve or restore microbial populations in the intestines. In this study, Leuconostoc probiotics was isolated from fermented gimchi and identified. Angelica dahurica, containing abundantly antioxidant activity, imperator, is a wildly grown species of angelica native. Before fermentation, total phenolics compound were $48.83{\pm}4.9GAE\;mg/g$ in the Angelica dahurica extract. After fermentation total phenolic compounds were $97.7{\pm}12.6GAE\;mg/g$. The total amount of phenol in the fermented product was 30.2% higher than that before fermentation. The total flavonoid content before fermentation was $9.86{\pm}4.3mg/g$ and the total flavonoid content was $37.17{\pm}7.4mg/g$ after fermentation, which was 82.3% higher than before fermentation. The DPPH radical scavenging activity, superoxide radical scavenging activity, hydroxy radical scavenging activity and $Fe^{{+}{+}}$ chelating antioxidative activity of the Angelica dahurica extract were $41.6{\pm}7.1%$, $65.7{\pm}8.4%$, $55.26{\pm}9.4%$ and $17.5{\pm}4.6%$, respectively. After fermentation, they were $60.3{\pm}12.6%$, $78.8{\pm}8.3%$, $56.9{\pm}4.9%$ and $36.6{\pm}8.9%$, respectively. Therefore, the present study suggests that the fermentation using the probiotics strain of the Angelica dahurica extract can be used as a functional health food and cosmetic material with increased antioxidant capacity.

• 한방재활의학과학회지
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• 제29권3호
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• pp.129-139
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• 2019

Objectives The purpose of this study is to investigate present status of cooperation through Korean medicine and Western medicine collaboration process. Methods We have established Korean medicine and Western medicine collaboration process to examine the status of cooperation. The medical records were investigated retrospectively, the general characteristics of gender, age of cooperative patients, classification of disease, frequency of medical treatment and type of insurance were analyzed. Results The Korean medicine and Western medicine collaboration process conducted in 4 stages, the convenience of patient movements is considered. A total of 245 people received cooperative medical treatment, 156 were out-patient department (OPD) patients and 89 were hospitalized patients, both group have more women than men. When classified as disease, OPD patients were most diagnosed with Sprain and strain of lumbar spine, while hospitalized patients were most diagnosed with lumbar and other intervertebral disc disorders with radiculopathy. A total of 72.7% patients were received cooperative medical treatment at once. In a survey of cooperative satisfaction, 68.5% of all medical staff responded positively to the treatment effect, and 68.6% said the need for cooperation was necessary. Conclusions As a result of this study, we were able to see the present status of cooperation, and through this, we found an improvement in the continuation of the cooperation. Based on the present study, It is hoped that a cooperative process will emerge that can improve the problems shown in this study.

• 한국미생물·생명공학회지
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• 제47권2호
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• pp.220-233
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• 2019

Bacterial lysates have become a common ingredient for natural health care. Lactic acid bacteria (LAB) could serve as potential candidates for lysate production: the lactic acids produced by LAB have been utilized for their moisturizing, antimicrobial, and rejuvenating effects, while other substances provide topical benefits and health effects for the skin. Our study aimed to obtain lysate from a LAB S. macedonicus MBF 10-2 through an optimized fermentation using the Response Surface Methodology. Strain MBF10-2 was cultivated in a 2L fermenter tank in de Man Rogosa and Sharpe (MRS) medium and in plant-based peptone modified MRS, i.e. Soy-peptone and Vegitone. The duration and the medium composition (dextrose and soy peptone or proteose peptone) were adjusted to obtain an optimum production of cell lysate. Central Composite Design was employed for Design Expert 7.0.0 by adjusting 3 factors: dextrose (1%, 1.5%, 2%, 2.5%, 3%), soy or proteose peptone (0.5%, 0.75%, 1%, 1.25% and 1.5%), and duration of fermentation (8, 10, 12 14, and 16 h for MRS-Soy peptone and 15, 17, 19, 21, and 23 h for MRS Vegitone). Bacteriocin-Like Inhibitor Substance activity of lysate and pH were used as indicators. The optimum condition for lysate production using MRS Soy Peptone and Vegitone are as follows: dextrose concentration 2.5%, plant-based peptone 1.25%, while optimum fermentation duration were 11.18 h (MRS Soy Peptone) and 17 h (MRS Vegitone) with a starter concentration of 10% at $OD_{600nm}$ $0.2{\pm}0.05$. However, the standard MRS medium produced better quality lysate compared to MRS plant-based peptones.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 추계학술대회
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• pp.687-689
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• 2014

스마트 폰의 보급과 의료 기술의 발달로 인해 건강의 패러다임이 치료에서 관리로 변화하였고 이에 맞춰 피트니스 사용자는 증가하고 있다. 하지만 피트니스 클럽 사용자는 자신에게 맞는 운동법을 찾기 위해 퍼스널 트레이너에게 값비싼 비용을 지불해야 하며 업체에서는 관리 시스템을 운영하기 위해서는 높은 비용이 요구되어 부담을 가중하고 있다. 본 논문에서는 공유기에 서버와 같은 확장이 가능한 OpenWrt를 중심으로 서버를 구성하고 클라이언트로 iOS 기반으로 모바일의 센서를 통해 사용자의 운동 기록을 수집하거나 사용자의 입력을 받는다. 이에 대한 데이터는 웹으로 전송하고 트레이너를 통해 체크 받는 시스템이며 이를 통해 사용자가 웨이트 트레이닝을 기록하고 이에 대한 피드백을 받을 수 있다.

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.500-505
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• 2019

Staphylococcus aureus is a round-shaped, gram-positive bacterium that can cause numerous infectious diseases ranging from mild infections such as skin infections and food poisoning to life-threatening infections such as sepsis, endocarditis and toxic shock syndrome. Various antibiotic-resistant strains of S. aureus have frequently emerged, threatening human lives significantly. Despite much research on the genetics of S. aureus, many of its genes remain unknown functionally and structurally. To counteract its toxins and to prevent the antibiotic resistance of S. aureus, our understanding of S. aureus should be increased at the proteomic scale. SAV0927 was first sequenced in an antibiotic resistant S. aureus strain. The gene is a conserved hypothetical protein, and its homologues appear to be restricted to Firmicutes. In this study, we determined the crystal structure of SAV0927 at $2.5{\AA}$ resolution. The protein was primarily dimeric both in solution and in the crystals. The asymmetric unit contained five dimers that are stacked linearly with ${\sim}80^{\circ}$ rotation by each dimer, and these interactions further continued in the crystal packing, resulting in a long linear polymer. The crystal structures, together with the network analysis, provide functional implications for the SAV0927-mediated protein network.

• 한국엔터테인먼트산업학회논문지
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• 제14권6호
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• pp.201-208
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• 2020

본 연구는 건강보험심사평가원의 청구 자료를 바탕으로 만65세 이상의 주진단이 무릎 관절증으로 슬관절 전치환술을 시행한 환자를 대상으로 의료기관 종별에 따라 재원일수에 영향을 주는 요인을 분석하였다. 연구결과 의료기관 종별에 따라 성별, 연령, 의료보장형태, 중증도, 거주지역 및 병상규모가 재원일수에 영향을 미치는 요인으로 분석되었다. 인구의 고령화로 인한 노인 인구의 증가와 이로 인한 노인 진료비 증가는 가계 및 국가 경제의 많은 부담으로 작용하는 시점에서 본 연구결과를 토대로 재원일수 단축효과와 함께 효율적인 병상운영을 도모해야 할 것이다. 뿐만 아니라 환자의 진료비 부담을 경감시키는 위한 체계적인 관리시스템을 도입하여 노인환자의 양질의 라이프케어를 위한 기초자료로 활용하는 데 본 연구의 의의가 있다.

• 대한정형도수물리치료학회지
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• 제28권3호
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• pp.21-32
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• 2022

Background: Instrument players are exposed to occupational musculoskeletal symptoms due to constant movement and strain to meet the needs of their audience. This can cause dissonance between the player and the instrument and can cause medical problems in the musculoskeletal system such as pain, tendinitis, muscle spasms, and joint hyperextension from overuse of body parts. Objective: The purpose of this study is to comparatively analyze the characteristics and occurrence of musculoskeletal symptoms in professional and student players, and to find out the awareness of the need for health care professionals and special exercise programs for orchestra players. Methods: The subjects were professional orchestral musicians 191 from the three symphony orchestras and music students 209 from the four universities volunteered to participate in this study; 393 subjects (98.25%) completed the questionnaire. symptom prevalence and related factors of playing-related musculoskeletal disorders (PRMDs) were surveyed with a self-administered questionnaire. Standardized Nordic Questionnaire was complemented by adding a visual-among scale and used to diagnose PRMD. Results: High prevalence of musculoskeletal complaints in both professional musicians (85.30%) and student musicians (82.30%). Professional musicians and student musicians also suffered the highest problem in shoulder and neck complaints. Significant differences were found between groups that recognized of necessity for specialized health professionals, professional organizations, and special programs for musicians. Conclusions: Professional musicians and student musicians, like orchestra players, are already exposed to overuse syndrome. However, student musicians lack awareness of the risk of injury compared to orchestra players. We suggest that an appropriate treatment management program for the prevention and early treatment of musculoskeletal system damage should be provided to performers at an early stage.