Browse > Article

Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis  

Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases)
Kim, Dong-Hee (Department of Internal Medicine, Seoul National University Hospital, Gangnam Health Care Center)
Lin, Aifen (Laboratory Center, Wenzhou Medical College-Affiliated Taizhou Hospital of Zhejiang Province, Linhai, Zhejiang Province)
Lee, Jo-Woon-Yi (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases)
Kim, Hyo-Jin (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases)
Ahn, Myoung-Hee (Department of Parasitology, Hanyang University College of Medicine)
Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases)
Publication Information
Parasites, Hosts and Diseases / v.46, no.1, 2008 , pp. 45-48 More about this Journal
Abstract
To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SOS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
Keywords
Toxoplasma gondii; Toxoplasmosis; Korean isolate-1 (KI-1); RH strain; Serodiagnosis; Antigen;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Dubey JP, Gennari SM, Labruna MB, Camargo LM, Vianna MC, Marcet PL, Lehmann T. Characterization of Toxoplasma gondii isolates in free-range chickens from Amazon, Brazil. J Parasitol 2006; 92: 36-40   DOI   ScienceOn
2 Jones JL, Kruszon-Moran D, Wilson M. Toxoplasma gondii infection in the United States, 1999-2000. Emerg Infec Dis 2003; 9: 1371-1374   DOI
3 Choi WY, Nam HW, Youn JH, Kim WS, Kim WK. Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary's Hospital and Cheju Medical Center. Korean J Parasitol 1989; 27: 171-175   DOI   ScienceOn
4 Choi WY, Nam HW, Kwak NH, Huh W, Kim YR, Kang MW, Cho SY, Dubey JP. Foodborne outbreaks of human toxoplasmosis. J Infec Dis 1997; 175: 1280-1282   DOI   ScienceOn
5 Kook J, Lee HJ, Kim BI, Yun CK, Guk SM, Seo M, Park YK, Hong ST, Chai JY. Toxoplasma gondii antibody titers in sera of children admitted to the Seoul National University Children's Hospital. Korean J Parasitol 1999; 37: 27-32   DOI   ScienceOn
6 Dubey JP, Frenkel JK. Toxoplasmosis of rats: a review, with considerations of their value as an animal model and their possible role in epidemiology. Vet Parasitol 1998; 77: 1-32   DOI   ScienceOn
7 Chai JY, Lin A, Shin EH, Oh MD, Han ET, Nam HW, Lee SH. Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea. Korean J Parasitol 2003; 41: 147-154   DOI   ScienceOn
8 Tenter AM, Heckeroth AR, Weiss LM. Toxoplasma gondii: from animals to humans. Int J Parasitol 2000; 30: 1217-1258   DOI   ScienceOn
9 Kasper LH. Isolation and characterization of a monoclonal anti-P30 antibody resistant mutant of Toxoplasma gondii. Parasite Immunol 1987; 9: 433-445   DOI   ScienceOn
10 Kook J, Oh SH, Yun CK, Chai JY. Effects of gamma-irradiation on intracellular proliferation of Toxoplasma gondii RH tachyzoites. Korean J Parasitol 1995; 33: 173-178   DOI
11 Diab MR, El-Bahy MM. Toxoplasma gondii: virulence of tachyzoites in serum free media at different temperatures. Exp Parasitol 2008; 118: 75-79   DOI   ScienceOn
12 Jones JL, Kruszon-Moran D, Wilson M, McQuillan G, Navin T, McAuley JB. Toxoplasma gondii infection in the United States: Seroprevalence and risk factors. Am J Epidemiol 2001; 154: 357-365   DOI   ScienceOn
13 Howe DK, Honore S, Derouin F, Sibley LD. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. J Clin Microbiol 1997; 35: 1411-1414   PUBMED
14 Choi WY, Nam HW, Youn JH, Kim DJ, Kong Y, Kang SY, Cho SY. Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzymelinked immunosorbent assay. Korean J Parasitol 1992; 30: 83-90   DOI
15 Lin A, Shin EH, Kim TY, Park JH, Guk SM, Chai JY. Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii. Korean J Parasitol 2005; 43: 27-32   과학기술학회마을   DOI   ScienceOn
16 Cavalcante GT, Aguilar DM, Camargo LM, Labruna MB, de Andrade HF, Meireles LR, Dubey JP, Thulliez P, Dias RA, Gennari SM. Seroprevalence of Toxoplasma gondii antibodies in humans from rural western Amazon, Brazil. J Parasitol 2006; 92: 647-649   DOI   ScienceOn