• Journal of Crop Science and Biotechnology
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• v.10 no.4
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• pp.277-280
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• 2007

Pepper fruit anthracnose, caused by Colletotrichum acutatum, results in serious yield loss and affects crop quality in many Asian countries, making it a disease of economic consequence. A source resistant to C. acutatum was identified by the AVRDC within the line Capsicum chinense Jacq. PBC932. The resistant breeding line C. annuum AR is the $BC_3F_6$ generation derived from C. chinense Jacq. PBC932. The inheritance of resistance to C. acutatum was analyzed in segregating populations derived from the two crosses HN 11$\times$AR and Daepoong-cho$\times$AR. Detached mature green fruits were inoculated using microinjection method. The disease response was evaluated as the disease incidence at 7 DAI. The segregation ratios of resistance and susceptibility to C. acutatum in the $F_2$ and $BC_R$ populations derived from the two crosses fit significantly to a 1:3 Mendelian model. This indicates that the resistance of AR to C. acutatum is controlled by a single recessive gene.

• Current Research on Agriculture and Life Sciences
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• v.20
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• pp.9-18
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• 2002

A total of 31 C. chinense lines selected from 2000 screening were tested again for resistance to P. capsid but resistance was not found in tile lines. A total of 26 selections from Korean land races tested 2001 spring were tested again for resistance to P. capsici, KC180, KC230, KC195 and KC194 showed moderate resistance to P. capsid. However, it was apparent on the basis of horticultural characteristics that KC180 and KC230 had been naturally crossed with AC2258 and CM334, respectively. CM334 and AC2248 seed lots that were increased in different years were taken out and tested for resistance to improve their genetic purity because the resistant genetic resources have been showing some off-types in tile population. Off-types began to be found in 1992 seed lots and tile proportion and degree of tile offs was increasing with time up to 2001. Plants true to the type in 1992 seed lots were selected and their inbred seeds were mass produced in a net cage in the greenhouse. AC2258 included in the experiment together was uniform. In 1995 seed lots of CM334, plants with resistance to P. capsici and low or no number of lateral branching at cotyledonary axil, although they were off from tile original CM334, were found and selection was applied to breed lines fixed in tile characters.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.286-293
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• 2012

The resistance to Tobamovirus in Capsicum spp. has been known to be controlled by five different alleles ($L^0$, $L^1$, $L^2$, $L^3$, and $L^4$) of L locus on the telomere of long arm of pepper chromosome 11. To develop a set of molecular markers differentiating all the alleles of L locus, we used five pepper differential hosts including Capsicum annuum Early California Wonder (ECW, $L^0L^0$), C. annuum Tisana ($L^1L^1$), C. annuum Criollo de Morelos 334 (CM334, $L^2L^2$), Capsicum chinense PI 159236 ($L^3L^3$), and Capsicum chacoense PI 260429 ($L^4L^4$). Developing a series of CAPS or SCAR markers specifically linked to the alleles was allowed by the sequence comparison of PCR amplicons of the $L^3$-linked markers (189D23M, A339, and 253A1R) and BAC sequences (FJ597539 and FJ597541) in the pepper differentials. Genotypes deduced by these markers in 48 out of 53 $F_1$ hybrids of commercial pepper varieties were consistent with their phenotypes by bioassay using Tobamovirus pathotypes ($P_0$, $P_1$, and $P_{1,2$). Consequently, these markers can be useful to differentiate L alleles and for breeding Tobamovirus resistance in pepper with marker-assisted selection.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.109.1-109
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• 2003

From the PMMV (pepper mild mottle virus)-inducible ESTs differentially expressed in Capsicum chinense PI257284, we isolated a full-length cDNA (CcPHZF： Capsicum chinense phenazine), encoding a phenazine biosynthesis protein which catalyzes the hydroxylation of phenozine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. Phenazine compound has been known to exhibit broad-spectrum of antibiotic activity against various species of bacteria and fungus. The entire region of CcPHZF is 879 bp in length and the open reading frame predicted a polypeptide of 292 amino acids. The homolog of CcPHZF is not Present in database except clones of AC004044 and NM100203 from Arabidopsis with 58 and 59％, respectively. Genomic Southern analysis indicated that the pepper genome contains a single copy of CcPHZF. The CcPHZF was strongly induced in the pepper leaves 3 days after PMMV treatment, when HR occurs on the leaf surface. Characterization of CcPHZF is underway to investigate if the CcPHZF is related to disease resistance against pathogens.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.68-68
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• 2018

A total of 47 core collections of pepper (Capsicum chinense Jacq.) conserved in National Agrobiodiversity Center (NAC) were studied under field condition at Jeonju. All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. Distribution of fruit characters (fruit length, width, and fruit wall thickness) among the accessions was positively skewed. Of the 47 accessions evaluated, 38.3% accessions had conical shaped fruits and mature fruit color was predominantly red (51.1%), orange (21.3%) and yellow (14.9%). Principal component analyses revealed that (i) 56.64% of the qualitative (fruit shape, color and fruit surface) variation and (ii) 89.42% of the quantitative (plant width, height and fruit maturity days) variation were explained by the first two components. Clustering revealed two groups and dendrogram revealed morphological variation among accessions. The phenotypic diversity exists in this core collections provide valuable information to improve agronomic traits in pepper breeding program.

• Molecules and Cells
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• v.27 no.2
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• pp.205-209
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• 2009

The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

• Korean Journal of Plant Tissue Culture
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• v.27 no.5
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• pp.367-373
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• 2000

We have constructed a molecular linkage map of chili pepper (Capsicum spp.) in an interspecific (C. annuum cv. TF68 x C. chinense cv. Habanero) F$_2$ population of 107 plants with 150 RFLP and 430 AFLP markers. The resulting linkage map consists of 11 large (206-60.3 cM) and 5 small (32.6- 10.3 cM) linkage groups cover-ing 1,320 cM with an average map distance between framework markers of 7.5 cM. Most (80%) of the RFLP markers were pepper-derived clones and these markers were evenly distributed across the genome. By using 30 primer combinations, 444 AFLP markers were generated in the F$_2$population. The majority of the AFLP markers clustered in each linkage group, although PstI/MseI markers were more evenly distributed than Eco RI/MseI markers within the linkage groups. Genes for biosynthesis of carotenoids and capsaicinoids were mapped on our linkage map. This map will provide the basis of studying secondary metabolites in pepper.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.7-11
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• 2001

A total of 238 accessions of peppers (Capsicum spp.) were evaluated for resistance to powdery mildew (Leveillula taurica (Lev.) Arn) in 1998-1999. KC604, 605, 606 and 608 of C. baccatum, KC616 of C. chinense, and KC638, 640, 641, 642, 643 and 644 of C. pubescens were highly resistant and remained disease-free. KC47-1 (PI244670), KC319-1, KC545, KC320 showed only mild infection compared with susceptible control group, 'Chungok' and others. Therefore, they were considered moderately resistant. Among them, KC47-1, KC319-1 and KC320 are sources of resistance to gray leaf spot caused by Stemphylium spp. while KC47-1 is also resistant to bacterial leaf spot (Xanthomonas campestris pv. vesicatoria (Doidge) Dye). Therefore, they may be used in breeding for multiple resistance to those diseases.

• The Plant Pathology Journal
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• v.28 no.1
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• pp.107-113
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• 2012

Trichomes are specialized epidermal structure having the functions of physical and chemical block against biotic and abiotic stresses. Several studies on $Capsicum$ species revealed that virus and herbivore resistance is associated with trichome-formation. However, there is no research on the structural characterization of trichomes developed on the epidermis of $Capsicum$ spp. Thus, this study attempts to charaterize the trichome morphologies in 5 species of $Capsicum$ using a Field Emission Scanning Electron Microscopy (FESEM). Six main trichome types were identified by their morphology under FESEM. Both glandular and non-glandular types of trichomes were developed on the epidermal tissues of $Capsicum$ spp. The glandular trichome were further classified into type I, IV and VII according to their base, stalk length, and stalk. Non-glandular trichomes were also classified into type II, III, and V based on stalk cell number and norphology. Almost all the species in $C.$ $chinense$ and $C.$ $pubescens$ had glandular trichomes. To our knowledge, this is the first study on classification of trichomes in the genus $Capsicum$ and, our results could provide basic informations for understanding the structure and function of trichomes on the epidermal differentiation and association with biotic stress tolerance.

• Horticultural Science & Technology
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• v.16 no.4
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• pp.503-507
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• 1998

This study was initiated to evaluate genetic relationship among various domestic and exotic pepper accessions using random amplified polymorphic DNA(RAPD) markers. The results suggested that the optimum conditions for PCR with random primers in Capsicum spp. could be obtained with 3mM of $MgCl_2$, 1.5U of Taq. DNA polymerase, 10ng of template DNA, $200{\mu}M$ of dNTPs, 200nM of random primer, and $42^{\circ}C$ of annealing temperature. Sixteen random primers showing high band intensity and reproducibility were selected from 80 random primers. Primers having 70% GC content were more effective in DNA amplification than primers having 60% GC content. The total 93 DNA bands including 71 polymorphic bands and 22 monomorphic bands were obtained with selected 16 random primers for 31 pepper cultivars and lines. About 4.4 polymorphic bands per primer were produced. Similarity coefficients were calculated by using 71 polymorphic bands and dendrogram based on the similarity coefficient showed clear classification of 31 peppers into three Capsicum species of Capsicum annuum, Capsicum chinense and Capsicum chacoense.