[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.1007/s10059-009-0025-z

Localization of 5S and 25S rRNA Genes on Somatic and Meiotic Chromosomes in Capsicum Species of Chili Pepper

Kwon, Jin-Kyung (Plant Genomics and Breeding Institute, Seoul National University)
Kim, Byung-Dong (Plant Genomics and Breeding Institute, Seoul National University)

Abstract

The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

Keywords

BAC; fiber-FISH; FISH; rDNA; pachytene;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 10 (Related Records In Web of Science)

Reference
Cited By KSCI

1	Kamisugi, Y., Nakayama, S., Nakajima, R., Ohtsubo, H., Ohtsubo, E., and Fukui, K. (1994). Physical mapping of the 5S ribosomal RNA genes on rice chromosome 11. Mol. Gen. Genet. 245, 13-138
2	Kang, B.-C., Yu, J.W., Lee, M.H., and Kim, B.-D. (1997). Applicability of AFLP on hot pepper genetic analysis. J. Kor. Soc. Hort. Sci. 38, 698-703
3	Maluszynska, J., and Heslop-Harrison, J.S. (1993). Physical mapping of rDNA loci in Brassica species. Genome 36, 774-781 DOI ScienceOn
4	Yoo, E.Y., Kim, S., Kim, Y.H., Lee, C.J., and Kim, B.-D. (2003). Construction of a deep coverage BAC library from Capsicum annuum, 'CM334'. Theor. Appl. Genet. 107, 540-543 DOI ScienceOn
5	Lee, H-L., Bae, I-H, Park, S-W., Kim, H-J., Min, W-K., Han, J-H., Kim, K-T., and Kim, B-D. (2009). Construction of an integrated pepper map using RFLP, SSR, CAPS, AFLP, WRKY, rRAMP, and BAC end sequences. Mol. Cells 27, 21-37 DOI ScienceOn
6	Park, Y.K., Park, K.C., Park, C.H., and Kim, N.S. (2000). Chromosomal localization and sequence variation of 5S rRNA gene in five Capsicum species. Mol. Cells 10, 18-24 DOI ScienceOn
7	De Jong, H. (1998). High resolution FISH reveals the molecular and chromosomal organization of repetitive sequences in tomato. Cytogenet. Cell Genet. 81, 104
8	Tanksley, S.D., Bernatzky, R., Lapitan, N.L., and Prince, J.P. (1988). Conservation of gene repertoire but not gene order in pepper and tomato. Proc. Natl. Acad. Sci. USA 85, 6419-6423 DOI ScienceOn
9	Ohmido, N., and Fukui, K. (1996). A new manual for fluorescence in situ hybridization (FISH) in plant chromosomes. Rice Genet. Newsl. 13, 89-96
10	Park, Y.K., Kim, B.D., Kim, B.S., Kim, K., Armstrong, C., and Kim, N.S. (1999). Karyotyping of the chromosomes and physical mapping of the 5S rRNA and 18S-26S rRNA gene families in five different species in Capsicum. Genes Genet. Syst. 74, 149-157 DOI ScienceOn
11	Peterson, D.G., Peterson, W.R., and Stack, S.M. (1998). Characterization of the tomato (Lycopersicon esculentum) genome using in vitro and in situ DNA reassociation. Genome 41, 346-356 DOI ScienceOn
12	Franze, P.F., Stam, M., Montijn, B., Hoopen, R.T., Weigant, J., Kooter, J.M., Oud, O., and Nanninga, N. (1996b). Detection of single-copy genes and chromosome rearrangements in Petunia Irybrida by fluorescence~in situ fluorescence in situ hybridization. Plant J. 9, 767-774 DOI ScienceOn
13	Hasterok, R., Wolny, E., Hosiawa, M., Kowalczyk, M., Kulak- Ksiazczyk, S., Ksiazczyk, T., Heneen, W.K., and Maluszynska, J. (2006). Comparative analysis of rDNA distribution in chromosomes of various species of Brassicaceae. Ann. Bot. 97, 205-216 DOI ScienceOn
14	Yi, G., Lee, J.M., Lee, S., Choi, D., and Kim, B-D. (2006). Exploitation of pepper EST-SSRs and an SSR-based linkage map. Theor. Appl. Genet. 114, 113-130 DOI ScienceOn
15	Ohmido, N., Akiyama, Y., and Fukui, K. (1998). Physical mapping of unique nucleotide sequences on identified rice chromosomes. Plant Mol. Biol. 38, 1043-1052 DOI ScienceOn
16	Schondelmaier, J., Schmidt, T., Heslop-Harrison, J.S., and Jung, C.(1997). Genetic and chromosomal localization of the 5S rDNA locus in sugar beet (Beta vulgaris L.). Genome 40, 171-175 DOI ScienceOn
17	Hanson, R.E., Islm-Faridi, M.N., Percival, E.A., Crane, C.F., Ji, Y., McKnight, T.D., Stelly, D.M., and Price, H.J. (1996). Distribution of 5S and 18-28S rDNA loci in a tetraploid cotton (Gossypium hirsutum L.) and its putative diploid ancestors. Chromosoma 105, 55-61 DOI
18	Moscone, E.A., Armando, M.L., and Friedrich, E. (1996). Fluorescent chromosome banding in the cultivated species of Capsicum (Solanaceae). Pl Syst. Evol. 202, 37-63 DOI ScienceOn
19	Kang, S-K., Lee, D.H, An, H-J., Park, J-H., Yun, S-H., Moon, Y-E., Bang, J-W., Hur Y.K., and Koo, D-H. (2008). Extensive chromosomal polymorphism revealed by ribosomal DNA and satellite DNA loci in 13 Citrus species. Mol. Cells 26, 319-322
20	Pedrosa-Harand, A., de Almeide, C.C., Mosiolek, M., Blair, M.W., Schweizer, D., and Guerra, M. (2006). Extensive ribosomal DNA amplification during Andean common bean (Phaseolus vulgrais L.) evolution. Theor. Appl. Genet. 112, 924-933 DOI ScienceOn
21	Moscone, E.A., Armando, M.L., and Friedrich, E. (1993). Giemsa Cbanded karyotypes in Capsicum (Solanaceae). Pl Syst. Evol. 186, 213-229 DOI
22	Murata, M., Heslop-Harrison, J.S., and Motoyoshi, F. (1997). Physical mapping of the 5S ribosomal RNA genes in Arabidopsis thaliana by multi-color fluorescence in situ hybridization with cosmid clones. Plant J. 12, 31-37 DOI ScienceOn
23	Franze, P.F., Alonso-Blanco, C., Liharska, T.B., Peeters, A.J.M., Zabel, P., and De Jong, H. (1996a). High-resolution physical mapping in Arabidopsis thaliana and tomato by fluorescence in situ hybridization to extended DNA fibers. Plant J. 9, 421-430 DOI ScienceOn
24	Moscone, E.A., Monika, B., Irma, E., Johann, G., Friedrich, E., and Armando, T.H. (2003). Analysis of nuclear DNA content in Capsicum (Solanaceae) by flow cytometry and Feulgen densitometry. Ann. Bot. 92, 21-29 DOI ScienceOn
25	Cai, Q., Zhang, D., Lui, Z.l., and Wang, X.R. (2006). Chromosomal localization of 5S and 18S rDNA in five species of subgenes Strobus and their implications for genome evolution of Pinus Ann. Bot. 97, 715-722 DOI ScienceOn

12	(2009) Genome Ribosomal DNA, heterochromatin, and correlation with genome size in diploid and polyploid North American endemic sagebrushes (<I>Artemisia,</I>Asteraceae) / 52 (12) , 1012
2	(2009) Journal of genetics Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid / 89 (2) , 163
6	Hyun Jung Kim. (2009) TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik Fine mapping of pepper trichome locus 1 controlling trichome formation in Capsicum annuum L. CM334 / 120 (6) , 1099
8	(2010) TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.) / 120 (8) , 1587
9	(2009) Genetica. : an international journal of genetics and evolution Distribution of 45S and 5S rDNA sites in 23 species of Eleocharis (Cyperaceae) / 138 (9) , 951
1	(2009) Genetics and molecular research Physical location of the carotenoid biosynthesis genes Psy and β-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae) / 10 (1) , 404
5	(2009) Tree Genetics & Genomes Genome size and cytogenetic characterization of three Algerian Retama species / 7 (5) , 987
None	(2011) BMC genomics Comparative analysis of pepper and tomato reveals euchromatin expansion of pepper genome caused by differential accumulation of <I>Ty3/Gypsy</I> -like elements / 12 (None) , 85
1	(2012) Molecules and cells SNP marker integration and QTL analysis of 12 agronomic and morphological traits in F8 RILs of pepper (Capsicum annuum L.) / 34 (1) , 25
None	(2009) Scientia horticulturae Karyotype analysis using FISH (fluorescence in situ hybridization) in Fragaria / 136 (None) , 95
2	(2009) Genetic resources and crop evolution Heterochromatin type, amount and distribution in wild species of chili peppers (Capsicum, Solanaceae) / 60 (2) , 693
1	(2014) Plant systematics and evolution Genome size and karyotype diversity in Solanum sect. Acanthophora (Solanaceae) / 300 (1) , 113
2	(2009) Plant systematics and evolution Karyotype of mitotic metaphase and meiotic diakinesis in non-heading Chinese cabbage / 300 (2) , 295
3	(2009) Molecular breeding : new strategies in plant improvement An EST-based linkage map reveals chromosomal translocation in Capsicum / 34 (3) , 963
22	(2009) Journal of experimental botany Tomato <I>Male sterile 10</I> <SUP><I>35</I></SUP> is essential for pollen development and meiosis in anthers / 65 (22) , 6693
6	(2009) Plant biosystems Comparative cytogenetic analysis of<I>Cestrum</I>(Solanaceae) reveals different trends in heterochromatin and rDNA sites distribution / 149 (6) , 976
2	(2009) Botanical journal of the Linnean Society The evolution of genome size and rDNA in diploid species of <I>C</I><I>henopodium s.l.</I> (Amaranthaceae) / 179 (2) , 218
2	(2016) Genome FISH and AgNor mapping of the 45S and 5S rRNA genes in wild and cultivated species of C<I>apsicum</I> (Solananceae) / 59 (2) , 95
1	(2009) Anais da Academia Brasileira de Ciências FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) / 88 (1) , 117
2	(2009) New Zealand journal of botany Differentiation of <I>Nolana</I> and <I>Sclerophylax</I> (Solanaceae) by means of heterochromatin and rDNA patterns / 55 (2) , 163
3	(2009) Plant biosystems Heterochromatin and rDNA patterns in <I>Solanum</I> species of the Morelloid and Dulcamaroid clades (Solanaceae) / 151 (3) , 539
1	(2019) Cytologia Physical Mapping of 5S rDNA in <I>Eucalyptus dunnii</I> Maiden and <I>Zea mays</I> L. by PRINS / 84 (1) , 77
9	(2009) Genes & genomics In silico mining and FISH mapping of a chromosome-specific satellite DNA in Capsicum annuum L. / 41 (9) , 1001
6	(2019) Plant biotechnology reports Genome divergence in <I>Brassica rapa</I> subspecies revealed by whole genome analysis on a doubled-haploid line of turnip / 13 (6) , 677
5	(2009) Plant biosystems Molecular cytogenetic evidence of hybridization in the “purple corolla clade of the genus <I>Capsicum</I>” (<I>C. eximium</I> × <I>C. cardenasii</I>) / 154 (5) , 685
4	(2009) Journal of plant research Tissue and/or developmental stage specific methylation of nrDNA in Capsicum annuum / 134 (4) , 841

1	Karyotype Analyses of Genetically Modified (GM) and Non-GM Hot Peppers by Conventional Staining and FISH Method / [Hwang, Yoon-Jung;Lee, Sung-Nam;Song, Kyung-A;Ryu, Kwang-Bok;Ryu, Ki-Hyun;Kim, Hyun-Hee;] / Horticulture, Environment, and Biotechnology : HEB
2	SNP Marker Integration and QTL Analysis of 12 Agronomic and Morphological Traits in $F_8$ RILs of Pepper (Capsicum annuum L.) / [Lu, Fu-Hao;Kwon, Soon-Wook;Yoon, Min-Young;Kim, Ki-Taek;Cho, Myeong-Cheoul;Yoon, Moo-Kyung;Park, Yong-Jin;] / Molecules and Cells
3	Medium Composition and Growth Regulator on Organogenesis Platycodon grandiflorum (Jacq.) A. DC. with Yellow Green Petals / [Kwon, Soo Jeong;Cho, Kab Yeon;Kim, Hag Hyun;] / Korean Journal of Plant Resources