• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 1999년도 추계학술발표회
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• pp.39-43
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• 1999

불포화대에서 LNAPL의 이동과 분포를 수치 모의를 통하여 예측하였다. 균질한 매질에서 LNAPL의 이동은 조립질 매질에서 빠르고, 세립질 매질에서 더 많은 면적으로 확산되며, 더 많은 LNAPL이 불포화대에 잔류한다. 조립질 매질내에 세립질층이 존재할 경우, 이 층이 지하수면으로부터 멀수록 LNAPL이 많이 포획된다. 조립질 매질에 세립질 또는 더 조립질인 매질이 렌즈 상으로 존재하는 환경에서는, LNAPL이 이들 렌즈를 통과하지 못한다. 불균질한 렌즈가 존재할 때의 LNAPL 분포를 초기조건으로 이용하여, 지하수 면의 수직 이동과 물의 침투에 따른 LNAPL의 이동을 모의하였다. 두 경우 모두 불포화대에 잔류되어 있던 LNAPL의 수직방향 이동이 증가되었다. 특히, 지하수면의 하강 시 LNAPL이 조립질 렌즈를 통해 이동하나, 세립질 렌즈를 통해서는 이동하지 못한다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2000년도 추계학술대회 논문집 학회본부 C
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• pp.583-585
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• 2000

This study reports the fluidic handling method using hydrophobic patterns inside PDMS microchannels. In order to obtain hydrophobic patterns, we pattern fluorcarbon(FC) film surfaces by lift-off process. FC films are deposited by spin coating method and plasma polymerization method. Hydrophobic surfaces are used as the barriers to control fluid flow. Injected liquid is spontaneously filled up inside PDMS-microchannels by the capillary action. Liquid flow stops when it meets hydrophobic regions which can be the barrier against fluid flow. Then, again, when liquid is pressurized externally, liquid can move toward another hydrophilic region by external air pressure. Contact angle analyses are performed on fluorocarbon films to estimate the wettability of film surfaces.

• Archives of Pharmacal Research
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• 제23권4호
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• pp.391-395
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• 2000

To establish the usefulness of oxytocin (OT) as an analgesic for women in delivery, the pharmacokinetic parameters and blood-brain barrier (BBB) permeability of [$^3H$] OT were obtained using an intravenous injection technique or the internal carotid artery perfusion/capillary depletion (ICAP/CDM) method. Brain uptake of OT was similar to that of sucrose, plasma space marker, indicating that OT has a poor BBB permeability. Moreover, the analgesic effects of OT injected through the jugular vein on nociception were evaluated by the tail-flick method. The antinociceptive effects of OT injected at a dose of 0.2 ${m}g/kg$or 2 ${m}g/kg$ were dose-dependent. In addition, the analgesic effects of OT on the CNS were unaffected by naloxone, a m-receptor antagonist. In a similar manner to the opioid system, OT may play a modulatory role in antinociception.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.106-106
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• 2003

Choline serves critical roles in the CNS both as a precursor of neurotransmitter and as an essential component of membrane phospholipids. The long-term maintenance of brain choline concentration is dependent on choline transport across the blood-brain barrier (BBB), And, we examined to elucidate the characteristics of transport of choline across the BBB using conditionally immortalized rat brain capillary endothelial cell line (TR-BBB) in vitro. The ［$^3$H］choline in TR - BBB was increased by time dependently, but independent on Na$\^$＋/, and the transport process is saturable with Michaelis-Menten constrant, Km of about 26 ${\mu}$M. The uptake of ［$^3$H］choline is susceptible for inhibition by various organic cationic compounds including hemicholinium-3, tetraethylammonium chloride (TEA) and $\ell$-carnitine. Also, we investigated the relationship of transport of choline and cationic drugs. The uptake of ［$^3$H］choline is inhibited by antioxidant, a-phenyl-n-tert-butyl nitrone (PBN) with IC$\sub$50/ of 1.2 mM. and by Alzheimer's disease therapeutics, such as acetyl $\ell$-carnitine, tacrine and donepezil. Also, choline uptake presented competitive inhibition with PBN, donepezil and acetyl $\ell$-carnitine in Lineweaver-Burk plot. In conclusion, TR-BBB cells express a saturable transport system for uptake of choline, and several cationic drugs may be transported into the brain by BBB choline transporter.

• 약학회지
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• 제42권2호
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• pp.196-204
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• 1998

The blood-brain barrier (BBB) of rats was modificated opening reversibly by infusing a hyperosmotic solution of arabinose (1.6 molal) into the right external carotid artery. Pre vious studies demonstrated that permeability was increased maxmmally in the first 15 min and remained slightly elevated at 1 hr. As control reference, saline was used. In the present study, to evaluate the effects of osmotic BBB opening on the BBB trasport according to hydrophilic or hydrophobic characteristics of drugs. And the differences of the uptakes of these compounds to right (treated osmotic opening) and left (untreated) hemispheres in same rats were compared each other following injection of 8 mCi per rat of $^{99m}Tc$-ethylene triamine pentaacetic acid (DTPA) as hydrophilic drug or 5mg/kg of phenytoin as hydrophobic drug mto the right external carotid artery of rats between two groups (1.6 molal arabinose vs saline). The uptakes of $^{99m}Tc$-DTPA and phenytoin in the right cerebral hemispheres were increased to about thirty three times and twice rather than those in the left cerebral heimspheres, respectively. And PAs (permeability X capillary surface area) were also increased from a control mean of 2.11${\times}10^{-4}$ (Untreated) to 6.98${\times}10^{-3}\;sec^{-1}$ (treated osmotic opening for $^{99m}Tc$-DTPA and 0.29 to 0.17 $sec^{-1}$ for phenytoin, respectively. From the results of present study, it is noted that osmotic opening of BBB is more effective in the brain delivery of hydrophilic drugs rather than that of hydrophobic drugs.

• 한방안이비인후피부과학회지
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• 제30권1호
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• pp.29-42
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• 2017

Objectives : Atopic dermatitis accompanies with severe pruritus and collapse of skin barrier, inflammation. Maifanite could be used as an ointment for skin disease. However, there have been few studies about maifanite uses for atopic dermatitis. We report the anti-inflammatory and promoting skin recovery effects of ionized maifanite on damaged skin barrier with experimentally elicited atopic dermatitis. Methods : Nc/Nga mice were divided into 3 groups: control group(CON), atopic dermatitis elicited group(AE group), ionized maifanite treated group after atopic dermatitis elicitation(MT group). After 5% SDS was applied D. pteronyssinus crude extract also applied for 3 weeks to elicit atopic dermatitis-like skin disease. MT group was treated for 3 weeks with ionized maifanite. Ionized maifanite was applied once a day and voluntarily administrated. AE group and control group were treated with normal saline in the same way. Results : In MT group, skin lesions like eczema were more improved than AE group. p-ERK1/2 positive reaction was reduced in MT group. MMP-9 and substance P positive reaction at dermal papillae was also reduced in MT group. With skin angiogram, capillary vessel decreased in MT group. Also, IL-4 positive reaction cell and STAT-6 positive reaction cell reduced more in MT group than in AE group. $NF-{\kappa}B$ p65 positive reaction cell and iNOS positive reaction cell also declined more in MT group than in AE group. Conclusions : It is supposed that ionized maifanite has anti-inflammatory effects on NC/Nga mice's atopic dermatitis with suppressing IL-4 production and Th2 cell differentiation, and controlling $NF-{\kappa}B$ activation.

• Journal of Animal Science and Technology
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• 제65권6호
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• pp.1205-1213
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• 2023

The goals of the present study were to develop a simple method for obtain highly purified pig sperm hyaluronidase (pHyase) and to assess its activity, function, and safety. In mammals, sperm-specific glycophosphatidylinositol (GPI)-anchored Hyase assists sperm penetration through the cumulus mass surrounding the egg and aids in the dispersal of the cumulus-oocyte complex. Recently, Purified bovine sperm hyaluronidase (bHyase) has been shown to enhance therapeutic drug transport by breaking down the hyaluronan barrier to the lymphatic and capillary vessels, thereby facilitating tissue absorption. Commercially available Hyase is typically isolated from bovine or ovine; which have several disadvantages, including the risk of bovine spongiform encephalopathy, low homology with human Hyase, and the requirement for relatively complex isolation procedures. This study successfully isolated highly purified pHyase in only two steps, using ammonium sulfate precipitation and fast protein liquid chromatography. The isolated Hyase had activity equal to that of commercial bHyase, facilitated in vitro fertilization, and effectively dissolved high molecule hyaluronic acid. This simple, effective isolation method could improve the availability of pHyase for research and clinical applications.

• 동의생리병리학회지
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• 제33권2호
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• pp.102-108
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• 2019

This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.

• 지질공학
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• 제13권1호
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• pp.51-65
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• 2003

불포화대에서 저밀도 비수용성유체의 이동과 분포를 모의하기 위하여 STOMP(Subsurface Transport over Multiple Phase) 시뮬레이터를 이용하였다. 균질한 매질에서의 저밀도 비수용성유체의 이동은 조립질 매질에서 빠르고, 세립질 매질에서는 불포화대에 더 많이 잔류한다. 조립질 매질 내에 세립질층이 존재할 경우, 이 층이 지하수면으로부터 멀수록 저밀도 비수용성유체가 불포화대에 더 많이 잔류된다. 조립질 매질에 세립질 또는 더 조립질인 매질이 렌즈 상으로 존재하는 경우에는 저밀도 비수용성유체가 이들 렌즈를 통과하지 못한다. 불균질한 렌즈가 존재할 때의 저밀도 비수용성유체의 분포를 초기조건으로, 지하수면의 변동에 따른 저밀도 비수용성유체의 이동을 모의하였다. 지하수위의 변동에 따라 불포화대에 잔류되어 있던 저밀도 비수용성유체의 수직방향 이동이 증가되었다. 특히, 지하수면의 하강 시 저밀도 비수용성유체가 조립질 렌즈를 통해 이동하나, 세립질 렌즈를 통해서는 이동하지 못한다. 일련의 수치실험의 결과로부터 유류 등의 저밀도 비수용성유체의 오염분포는 매질의 불균질성 그리고 지하수위 변동과 같은 수리지질학특성에 의해 크게 영향을 받을 수 있음을 알 수 있다.

• 한국지반공학회논문집
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• 제31권1호
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• pp.53-64
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• 2015

본 연구는 도심지 내 집중 강우 특성을 고려한 투수성 보도블록의 배수 특성을 알아보기 위하여 2 가지 골재크기에 대하여 강도, 투수성, 증발 및 배수실험을 실시하였다. 강도의 경우 골재크기와 무관하게 한국산업표준(KS) 규격을 만족시킨 반면, 작은 골재로 구성된 투수성 보도블록의 경우 단위시간당 유량이 작은 경우에도 표면유출이 발생하였다. 큰 골재는 초기 우수조건에서의 배수는 원활한 반면 일정시간이 지난 후 동일한 유량의 배수실험에서 표면유출이 관찰되었다. 이는 간극 내부의 물이 투수성을 저감시키는 역할로 작용한 것으로, 투수성 보도블록의 투수성과 배수 성능은 간극비나 간극연결도 만으로 결정되는 것이 아니고 시간에 따른 증발, 선행우수조건에 의해 좌우됨을 나타낸다. 또한 초기 포화도보다는 이중선형 형태의 시간당 증발 양상과 유입량과 증발로 인한 내부 포화도 변화가 표면유출에 미치는 영향이 큰 것으로 나타났다.