• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.132-134
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• 2005

The CC chemokine, monocyte chemoattractant protein-1 (MCP-1), plays a crucial role in the initiation of atherosclerosis and has direct effects that promote angiogenesis. To develop a specific inhibitor for MCP-1-induced angiogenesis, we performed in vitro selection employing phage display random peptide libraries. Most of the selected peptides were found to be homologous to the second extracellular loops of CCR2 and CCR3. We synthesized the peptide encoding the homologous sequences of the receptors and tested its effect on the MCP-1 induced angiogenesis. Surface Plasmon Resonance measurements demonstrated specific binding of the peptide to MCP-1 but not to the other homologous protein, MCP-3. Flow cytometry revealed that the peptide inhibited the MCP-1 binding to THP-1 monocytes. Moreover, CAM and rat aortic ring assays showed that the peptide inhibited MCP-1 induced angiogenesis. Our observations indicate that the MCP-1-binding peptide exerts its anti-angiogenic effect by interfering with the interaction between MCP-1 and its receptor.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1997년도 제26회 추계학술대회
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• pp.225-231
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• 1997

This paper reports on the theoretical study of the delivery pressure ripples of a positive displacement vane pump which is widely used for automobile power steering. Pressure ripples occur due to the flow tipples which induced cam ring profiles and reverse flow from the delivery ports. In this paper, the mathematical model for analyzing the pressure ripples has been presented, and set of the differential equationshave been solved using the Runge-Kutta method. As the results of analysis, instant ideal flow ripples, internal pressure, delivery pressure ripples, and delivery flow ripples have been presented. Internal pressure was related to delivery pressure variations, and amplitude of pressure tipples was increased with rotational speed and delivery pressure.

• 한국정밀공학회지
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• 제26권4호
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• pp.55-63
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• 2009

This paper reports on the theoretical and experimental study of the pressure ripples in a pressure unbalanced type vane pump which have widespread use in industry. Because they can infinitely vary the volume of the fluid pumped in the system by a control. Pressure ripples occur due to the flow ripples induced by geometry of side plate, leakage flow, reverse flow from the outlet volume produced by pressure difference between pumping chamber and outlet volume when the pumping chamber connected with the outlet volume. In this paper, we measured the pressure variation of a pumping chamber, reaction force on a cam ring, the mathematical model for analyzing the pressure ripples which included vane detachment and fluid inertia effects in notch area has been presented, and was applied to predict the level and the wave form of the pressure ripples according to operating conditions.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.262-269
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• 2005

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1998년도 제27회 춘계학술대회
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• pp.277-285
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• 1998

This paper presents the deformation characteristics of the moving pressure plate in a balanced type vane pump that widely used automotive power steering systems. Moving pressure plate can control the clearance between rotor and plate in accordance with load pressure variation; it always guarantees that pump to have optimal volumetric efficiency. In this paper, firstly, we calculate the acting force on the pressure plate, which is used to determine the angular position and load condition for analyzing the deformation of pressure plate. Secondary, finite element method is used for the deformation analysis. As results of acting force analysis, it is found that maximum difference of forces occurs at angular position 28$\circ$ from the small arc center of cam ring and load pressure is a dominant factor to affect acting force variation. The deformation of pressure plate increases as load pressure increases. At high load pressure, the deformation of pressure plate becomes larger than the initial clearance between rotor and plate. Therefore, it is required to design the plate for controlling the deformation.

• 한국가스학회지
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• 제23권4호
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• pp.40-45
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• 2019

논문은 대형트럭의 화재에 관련된 고장사례를 고찰한 논문이다. 첫 번째 사례는 6번 실린더 라이너 상단부와 실린더 헤드 사이에 접촉하는 D링부의 변형으로 헤드 가스켓부의 기밀불량 현상으로 냉각수가 누출하여 엔진이 과열되었다. 이 열이 에어크리너 주변 배선에 전달되어 쇼트(short)에 의해 화재가 발생된 것을 확인하였다. 두 번째 사례는 자동차의 리어(Rear) 4축 브레이크 라이닝 과다마모로 브레이크 작동 S 캠(Cam)이 리턴되지 않아 브레이크 라이닝이 드럼과의 계속적인 마찰에 의한 마찰열에 의해 화재가 발생된 것을 확인하였다. 세 번째 사례는 엔진 점검을 위해 캡을 틸팅하였을 때 틸팅 모터의 과부하로 배선의 단락현상이 발생하여 이 불꽃에 의해 화재가 발생한 것을 확인하였다. 따라서, 대형트럭의 화재는 철저한 관리를 하여 화재발생을 최소화하여야 한다.

• Bulletin of the Korean Chemical Society
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• 제25권9호
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• pp.1331-1335
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• 2004

Angiogenesis is essential for the growth and persistence of solid tumors. Their metastases, anti-angiogenesis could lead to the suppression of tumor growth. One of the main strategies of cancer treatment is developing molecules of anti-angiogenic activity. In this study, two angiogenic inhibitors, Ang3 (KLFDF) and Ang4 (XLFDF) derived from KLYDY, which is the sequence of angiostatin active sites kringle 5, were designed and synthesized. Previously we reported the activities and structures of two inhibitors, Ang1 (KLYDY) and Ang2 (KLWDF). In order to investigate the effect of Phe substitution, Ang3 was designed with a sequence of KLFDF. In order to reduce conformational flexibility of side chain in Lys, Ang4 was designed with a sequence of XLFDF, where X has amino substituted phenyl ring. Solution structures of those inhibitors were investigated using NMR spectroscopy and their activities as angiogenesis inhibitors were studied. Ang1 and Ang2 show angiogenic activities, while Ang3 and Ang4 have no activities and have extended structures compared to Ang1 and Ang2. Therefore, Phe rings do not have effective hydrophobic interactions with other aromatic residues in Ang3 and Ang4. The representative structure of Ang2 has a stable intramolecular hydrogen bond. Therefore, intramolecular hydrogen bonding might be more important in stabilizing the structure than the hydrophobic interactions in these inhibitors. More rigid structure, which can be expected to have higher activities and better match with the receptor bound conformations, can be obtained with a constrained cyclic structure. Further peptidomimetic approaches should be tried to develop angiogenesis inhibitors.