Browse > Article

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium  

Ahn Jae-Il (Department of Chemical and Biochemical Engineering, Dongguk University)
Jang In-Keun (Department of Chemical and Biochemical Engineering, Dongguk University, Department of Opthalmology, Chung-Ang University Hospital)
Lee Doo-Hoon (Department of Chemical and Biochemical Engineering, Dongguk University)
Seo Young-Kwon (Department of Chemical and Biochemical Engineering, Dongguk University)
Yoon Hee-Hoon (Department of Chemical and Biochemical Engineering, Dongguk University)
Shin Youn-Ho (Department of Chemical and Biochemical Engineering, Dongguk University)
Kim Jae-Chan (Department of Opthalmology, Chung-Ang University Hospital)
Song Kye-Yong (Department of Pathology, College of Medicine, Chung-Ang University)
Lee Hee-Gu (Molecular and Cell Biology Research Division, Korea Research Institute of Bioscience and Biotechnology)
Yang Eun-Kyung (R&D Center, Bioland Ltd.,)
Kim Ki-Ho (R&D Center, Bioland Ltd.,)
Park Jung-Keung (Department of Chemical and Biochemical Engineering, Dongguk University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.3, 2005 , pp. 262-269 More about this Journal
Abstract
Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.
Keywords
cornea; epithelium; amniotic membrane; teflon ring;
Citations & Related Records

Times Cited By Web Of Science : 10  (Related Records In Web of Science)
Times Cited By SCOPUS : 8
연도 인용수 순위
1 Yang, E. K., H. H. Yoon, D. H. Lee, and J. K. Park (2000) Assessment of toxic potential of industrial chemicals using a cultured human bioartificial skin model: Production of interleukin 1 alpha and hydroxyeicosatetraenoic acids. Skin Pharmacol. Appl. Skin Physiol. 13: 246-257   DOI   ScienceOn
2 Park, Y. G. and H. W. Ryu (2005) Evaluation methods and design for bioartificial liver based on perfusion model. Biotechnol. Bioprocess Eng. 10: 9-15   DOI   ScienceOn
3 Koizumi, N., T. Inatomi, T. Suzuki, C. Sotozono, and S. Kinoshita (2001) Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 108: 1569-1574   DOI   ScienceOn
4 Minami, Y., H. Sugihara, and S. Oono (1993) Reconstruction of corneal in three-dimensional collagen gel matrix culture. Invest. Ophthalmol. Vis. Sci. 34:. 2316-2324
5 Shapiro, M. S., J. Friend, and R. A. Thoft (1981) Corneal re-epithelialization from the conjunctiva. Invest. Ophthalmol. Vis. Sci. 21: 135-142
6 Noguchi, Y., Y. Uchida, T. Endo, H. Ninomiya, A. Nomura, T. Sakamoto, Y. Goto, S. Haraoka, T. Shimokama, T. Watanabe, and S. Hasegawa (1995) The induction of cell differentiation and polarity of tracheal epithelium cultured on the amniotic membrane. Biochem. Biophys. Res. Commun. 210: 302-309   DOI   ScienceOn
7 Kim, J. C. and S. C. G. Tseng (1995) Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea 14: 473-484   DOI
8 Lindberg, K., M. E. Brown, H. V. Chaves, K. R. Kenyon, and J. G. Rheinwald (1993) In vitro propagation of human ocular surface epithelial cells for transplantation. Invest. Ophthalmol. Vis. Sci. 34: 2672-2679
9 Stark, H. J., M. Baur, D. Breitkreutz, N. Mirancea, and N. E. Fusenig (1999) Organotypic keratinocyte cocultures in defined medium with regular epidermal morphogenesis and differentiation. J. Invest. Dermatol. 112: 681-691   DOI   ScienceOn
10 Yang, E. K., Y. K. Seo, and J. K. Park (2000) Development and application of bioartificial skin. Int. J. Artif. Organ 23: 597-600   DOI
11 Coulomb, B., C. Lebreton, B. A. Dubertret, and L. Dubertret (1989) Influence of human dermal fibroblasts on epidermalization. Soc. Invest. Dermatol. 92: 122-125   DOI   ScienceOn
12 Griffith, M., R. Osborne, R. Munger, X. Xiong, C. J. Doillon, N. L. C. Laycock, M. Hakim, Y. Song, and M. A. Watsky (1999) Functional human corneal equivalents constructed from cell lines. Science 286: 2169-2172   DOI   PUBMED   ScienceOn
13 Quinby, W. C., H. C. Hoover, M. Scheflan, P. T. Walters, S. A. Slavin, and C. C. Bondoc (1982) Clinical trials of amniotic membranes in burn wound care. Plast. Reconstr. Surg. 70: 711-716   DOI   ScienceOn
14 Kim, J. S., J. C. Kim, B. K. Na, J. M. Jeong, and C. Y. Song (2000) Amniotic membrane patching promotes healing and inhibits proteinase activity on wound healing following acute corneal alkari burn. Exp. Eye Res. 70: 329- 337   DOI   ScienceOn
15 Daniels, J. T., J. K. G. Dart, S. J. Tuft, and P. T. Khaw (2001) Corneal stem cells in review. Wound Rep. Reg. 9: 483-494   DOI   ScienceOn
16 Nakamura, T., M. Yoshitani, H. Rigby, N. J. Fullwood, W. Ito, T. Inatomi, C. Sotozono, T. Nakamura, Y. Shimizu, and S. Kinoshita (2004) Sterilized freeze-dried amniotic membrane: A useful substrate for ocular surface reconstruction. Invest. Ophthalmol. Vis. Sci. 45: 93-99   DOI   ScienceOn
17 Seo, Y. K., D. H. Lee, Y. H. Shin, B. Y. You, K. M. Lee, K. Y. Song, S. J. Seo, S. J. Whang, Y. J. Kim, E. K. Yang, C. S. Park, I. S. Chang, and J. K. Park (2003) Development of isolation and cultivation method for outer root sheath cells from human hair follicle and construction of bioartificial skin. Biotechnol. Bioprocess Eng. 8: 151-157   DOI   ScienceOn
18 Mohammad, J., J. Shenaq, E. Rabinovsky, and S. Shenaq (2000) Modulation of peripheral nerve regeneration: A tissue- engineering approach. The role of amnion tube nerve conduit across a 1-centimeter nerve gap. Plast. Reconstr. Surg. 105: 660-666   DOI   ScienceOn
19 Grueterich, M., E. M. Espana, and S. C. G. Tseng (2003) Ex vivo expansion of limbal epithelial stem cells:amniotic membrane serving as a stem cell niche. Surv. Ophthalmol. 48: 631-646   DOI   ScienceOn
20 Farazdaghi, M., J. Adler, and S. Farazdaghi (2001) Electron microscopy of human amniotic membrane. pp. 149- 171. In: A. Nather (ed.). The Scientific Basis of Tissue Transplantation. World Scientific Publishing Co., New jersey, USA
21 Zhang, X., K. M. Klueber, Z. Guo, C. Lu, and F. J. Roisen (2004) Adult human olfactory neural progenitors cultured in defined medium. Exp. Neurol. 186: 112-123   DOI   ScienceOn
22 Tseng, S. C. G. (1996) Regulation and clinical implications of corneal epithelium stem cells. Mol. Biol. Rep. 23: 47-58   DOI   PUBMED   ScienceOn
23 Kruszewski, F. H., T. L. Walker, and L. C. Dipasquale (1997) Evaluation of a human corneal epithelial cell line as an in vitro model for assessing ocular irritation. Fundam. Appl. Toxicol. 36: 130-140   DOI   ScienceOn
24 Ahn, J. I., I. K. Jang, Y. K. Seo, H. H. Yoon, D. H. Lee, Y. H. Shin, J. C. Kim, K. Y. Song, H. K. Lee, E. K. Yang, K. H. Kim, and J. K. Park (2003). Reconstruction of rabbit limbal corneal epithelial cells on lyophilized amniotic membrane. The Tissue Engineering Society International 6th Annual International Conference and Exposition. December 10-13. Orlando, USA
25 Subrahmanyam, M. (1995) Amniotic membrane as a cover for microskin grafts. Br. J. Plast. Surg. 48: 477-478   DOI   PUBMED   ScienceOn
26 Koizumi, N., L. J. Cooper, N. J. Fullwood, T. Nakamura, K. Inoki, M. Tsuzuki, and S. Kinoshita S (2002) An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture. Invest. Ophthalmol. Vis. Sci. 43: 2114-2121
27 Koizumi, N., T. Inatomi, A. J. Quantock, N. J. Fullwood, A. Dota, and S. Kinoshita (2000) Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits. Cornea 19: 65-71   DOI   ScienceOn
28 Koizumi, N., N. J. Fullwood, G. Bairaktaris, T. Inatomi, S. Kinoshita, and A. J. Quantock A (2000) Cultivation of corneal epithelial cells on intact and denuded human amniotic membrane. Invest. Ophthalmol. Vis. Sci. 41: 2506-2513
29 Ti, S. E., D. Anderson, A. Touhami, C. Kim, and S. C. G. Tseng (2002) Factors affecting outcome following transplantation of ex vivo expanded limbal epithelium on amniotic membrane for total limbal deficiency in rabbits. Invest. Ophthalmol. Vis. Sci. 43: 2584-2592
30 Kahn, C. R., E. Young, I. H. Lee, and J. S. Rhim (1993) Human corneal epithelial primary cultures and cell lines with extended life span: in vitro model for ocular studies. Invest. Ophthalmol. Vis. Sci. 34: 3429-3441
31 Park, J. K. (2003) Utilization of amniotic membrane as a matrix for tissue engineering. The Asia Pacific Biochemical Engineering Conference 03. November 30-December 4. Brisbane, Australia
32 Jasinkowski, N. L. and J. L. Cullum (1984) Human amniotic membrane as a wound dressing. AORN J. 39: 894- 899   DOI   ScienceOn
33 Park, J. K., Y. K. Seo, J. I. Ahn, I. K. Jang, J. C. Kim, K. Y. Song, and E. K. Yang (2002) Application of amniotic membrane as a scaffold for tissue engineering. The 9th International Conference on Tissue Banking ASIA Pacific Association of Surgical Tissue Banks. September 28-30. Seoul, Korea
34 Park, J. K., J. I. Ahn, I. K. Jang, J. B. Ju, J. C. Kim, K. Y. Song, H. G. Lee, E. K. Yang, and K. H. Kim (2004) Reconstruction of lamellar bioartificial cornea using lyophilized amniotic membrane and collagen scaffold. The Association for Research in Vision and Ophthalmology 2004 Annual Meeting. April 25-29. Fort Lauderdale, USA
35 Horch, R. E., M. Debus, G. Wagner, and G. B. Stark (2001) Cultured human keratinocytes on type I collagen membranes to reconstitute the epidermis. Transplant. Proc. 33: 642-644   DOI   ScienceOn
36 Honavar, S. G., A. K. Bansal, V. S. Sangwan, and G. N. Rao (2000) Amniotic membrane transplantation for ocular surface reconstruction in Stevens-Johnson Syndrome. Ophthalmology 107: 975-979   DOI   ScienceOn
37 Araki-Sasaki, K., Y. Ohashi, T. Sasabe, K. Hayashi, H. Watanabe, Y. Tano, and H. Handa (1995) An SV40- immortalized human corneal epithelial cell line and its characterization. Invest. Ophthalmol. Vis. Sci. 36: 614-621
38 Park, Y. G., T. Tosha, S. Fujita, B. Zhu, H. Iwata, and H. W. Ryu (2003) Effect of low temperature preservation and cell density on metabolic function in a bioartificial liver. Biotechnol. Bioprocess Eng. 8: 41-46   DOI   ScienceOn
39 Yang, E. K., Y. K. Seo, H. H. Youn, D. H. Lee, S. N. Park, and J. K. Park (2000) Tissue engineered artificial skin composed of dermis and epidermis. Artif. Organ 24: 7-17   DOI   ScienceOn
40 Tsai, R. J., L. Li, B. S. Chen, and J. Chen (2000) Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. New England J. Medicine 343: 86-93   DOI   ScienceOn
41 Nakamura, T., K. Endo, L. J. Cooper, N. J. Fullwood, N. Tanifuji, M. Tsuzuki, N. Koizumi, T. Inatomi, Y. Sano, and S. Kinoshita (2003) The successful culture and autologous transplantation of rabbit oral mucosal epithelial cells on amniotic membrane. Invest. Ophthalmol. Vis. Sci. 44: 106-116   DOI   ScienceOn
42 Park, J. K., J. I. Ahn, I. K. Jang, Y. H. Shin, J. H. Oh, J. C. Kim, K. Y. Song, H. K Lee, D. Y. Yoon, H. R. Boo, E. K. Yang, and K. H. Kim (2002) Culture of rabbit limbal corneal epithelial cells on lyophilized amniotic membrane. YABEC Symposium 2002. November 10-12. Taipei, Taiwan
43 Rosdy, M. and L. C. Clauss (1990) Terminal epidermal differentiation of human keratinocytes grown in chemically defined medium on inert filter substrates at the air-liquid interface. J. Invest. Dermatol. 95: 409-414   DOI   ScienceOn
44 Tyszkiewicz, J. T., I. A. Uhrynowska-Tyszkiewicz, A. Kaminski, and A. Dziedzic-Goclawska (1999) Amnion allografts prepared in the central tissue bank in Warsaw. Ann. Transplant. 4: 85-90