• Title/Summary/Keyword: Calcitonin

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Effects of Amydae Carapax on Bone Metabolism in Ovariectomized Rats (난소 절제 흰쥐의 골대사에 미치는 별갑의 영향)

  • 박종혁;윤철호;정지천
    • The Journal of Korean Medicine
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    • v.23 no.3
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    • pp.54-62
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    • 2002
  • Objectives : This study was undertaken to investigate the effects of Amydae Carapax (AC) on parameter related to bone metabolism in ovariectomized rats. Methods : We measured alkaline phosphatase activity and contents of estrogen, calcium, hydroxyproline, osteocalcin, calcitonin and parathyroid hormone after the ovariectomized rats were treated with AC for 30 days. Results : Serum estrogen, calcium and calcitonin contents in ovariectomized rats significantly decreased, but increased after AC treatment. [Significant increase of serum alkaline phosphatase activity, parathyroid hormone activity and osteocalcin content in ovariectomized rats was remarkably decreased by AC treatment. Increase of urinary calcium and hydroxyproline content in ovariectomized rats was decreased by AC treatment.] Conclusions : These results shows that AC has the ability to counteract abnormal calcium metabolic processes due to sex hormone inequality, promoting bone absorption and inhibiting bone formation.

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Peptide Amidation: Production of Peptide Hormones in vivo and in vitro

  • Kim, Kyun-Hwan;Baik L. Seong
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.4
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    • pp.244-251
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    • 2001
  • Over half of all biologically active peptide and peptide hormones are $\alpha$-amidated at their C-terminus, which is essential for their full biological activities. Amidation is accomplished through the sequential reaction of the two enzymes encoded by the single bifunctional, peptidyl-glycine $\alpha$-amidating monooxygenase (PAM or an $\alpha$-amidating enzyme). PAM catalyze the forma - tion of a peptide amide from peptide precursors that include a C-terminal glycine, and requires copper molecular oxygen and ascorbate. PAM is the only enzyme that produces peptide amides in vivo. However various strategies utilizing PAM, carboxypeptidase-Y enzymes, and chemical syn-thesis have been developed for producing peptide amides in vitro. The growing need and impor-tance of peptide amide drugs has highlighted the necessity for a efficient in vitro amidating sys-tem for industrial application for the production of peptide hormones, like calcitonin and oxytocin. This review presents the current situation regarding amidation with a special emphasis on the in-dustrial production or peptide hormones.

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Effects of Yuhyangjeongtong-san on Fracture Healing in Rats

  • Kim, Ki-Tae;Jo, Na-Young
    • The Journal of Korean Medicine
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    • v.40 no.4
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    • pp.61-71
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    • 2019
  • Objectives: The purpose of this study is to determine the effect of Yuhyangjeongtong-san on the recovery of tibial fractures in rats. Methods: In this study, osteocalcin and Calcitonin, CTX-2, TGF-β and BMP-2, which are used as indicators of bone formation, were analyzed after hematologic fractures using experimental rats. In addition, the fracture union process was confirmed using X-rays. Results: Osteocalcin, Calcitonin and BMP-2 showed a significant increase compared with the control at 4 weeks. CTX-2 and TGF-β showed a significant increase compared with the control at 3 weeks. On X-ray, YJS treated group, as the experiment progressed, the boundary line became blurred, the bone outline was clearly visible, and the fracture recover was progressing. Conclusion: The findings suggest that YJS can play a significant role in the repair of fractures. Therefore YJS is likely to be used to treat fractures.

Normal Anxiety, Fear and Depression-related Behaviors in Mice Lacking ${\alpha}-Calcitonin$ Gene-Related Peptide

  • Lee, Jong-Ho
    • The Korean Journal of Physiology and Pharmacology
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    • v.6 no.6
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    • pp.299-304
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    • 2002
  • Calcitonin gene-related peptide (CGRP) expressing neurons are distributed widely throughout the central and peripheral nervous systems. Due to its distribution and pharmacological studies, CGRP has been implicated to be involved in anxiety, fear and depression. In this study, ${\alpha}CGRP-knockout$ mice were used to assess the consequences of removing this neuropeptide to the mice behaviors. ${\alpha}CGRP-knockout$ mice performed equally as well as wild type mice in the light-dark transition test and in the elevated plus maze test of anxiety. ${\alpha}CGRP-null$ mice behaved similarly as wild-type mice in the Porsolt swim test of depression. They also exhibited normal learning and memory in the fear conditioning tasks. It is concluded that ${\alpha}CGRP$ is not essential for mice to be able to perform these tests, despite the presence of ${\alpha}CGRP$ in the relevant regions of the brain.

Effects of Taxilli Ramulus Extract on Bone Metabolism of Ethanol Treated Rats (상기생이 ethanol을 장기 투여한 흰쥐의 골 대사에 미치는 영향)

  • 정주화;정지천
    • The Journal of Korean Medicine
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    • v.22 no.4
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    • pp.1-9
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    • 2001
  • Objectives : To investigate the effect of Taxilli Ramulus (TR) extract on bone metabolism of ethanol-treated animal model. Methods : The changes of serum calcium, calcitonin, estrogen level, a1ka1ine phosphatase activity, osteocalcin, parathyroid hormone content and urine calcium level were observed with ethanol treatment for 60 days. The results were compared with an ethanol- TR extract double treatment group. Results : We observed increment of serum osteocalcin, parathyroid hormone content, alkaline phosphatase activity and urine calcium level by chronic ethanol feed and they were recovered to near normal level with Taxilli Ramulus extract treatment. Weight gain, serum calcium level, calcitonin and estrogen content were remarkably reduced with ethanol treatment and their levels were normalized by Taxilli Ramulus extract. Conclusions : These results showed that Taxilli Ramulus extract have the ability to recover to normal in the body an abnormal calcium metabolism process due to external factors. These results suggested that Taxilli Ramulus extract have preventive effects on calcium concentration loss and osteoporosis.

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Metastatic thyroid C-cell carcinoma in a beagle dog

  • Lee, So-Ra;Lee, Mi-Kyoung;Youp, Kyoung-A;Lee, Ah-Ra;Won, Sung-Jun;Kim, Myung-Chul;Kim, So-Yeon;Kim, Yongbaek
    • Korean Journal of Veterinary Research
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    • v.53 no.3
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    • pp.189-192
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    • 2013
  • An adult beagle dog was presented with a cervical mass detected by palpation and computed tomography. Fine needle aspirates revealed numerous epithelial cells with plasmacytoid appearance and frequent naked nuclei. Histologically, the mass consists of multiple packets of neoplastic cells and extensive areas of necrosis and fibrosis. Neoplastic cells were also found in submandibular lymph nodes. Immunohistochemistry showed that neoplastic cells were positive for calcitonin and negative for thyroglobulin. Based on these findings, the cervical mass was diagnosed as thyroid C-cell carcinoma. Almost one year after the surgical excision, the dog remains healthy without any symptom of recurrence or metastasis.

Effects of Rhizoma Davalliae on Bone Metabolism in Ovariectomized Rats (난소 절제 흰쥐의 골대사에 미치는 골쇄보(骨碎補)의 영향)

  • Kim, Kwang-Jin;Jeong, Ji-Cheon
    • The Journal of Internal Korean Medicine
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    • v.22 no.2
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    • pp.175-181
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    • 2001
  • Objectives : This study was undertaken to study the action mechanism of Rhizoma Davalliae (RD) at parameter related to regulating bone density. Methods : We measured alkaline phosphatase activity and the contents : calcium, hydroxyproline, osteocalcin, calcitonin, and parathyroid hormone after the ovariectomized rats had been treated with RD for 30 days. Results : The following changes occurred: First, the serum calcium content and the calcitonin content, which decreased in ovariectomized rats, increased with RD treatment. Second, the serum alkaline phosphatase activity, the parathyroid hormone ativity, the osteocalcin content, the urinary calcium content, and the hydroxyprolin content-which increased in ovariectomized rats-decreased with RD treatment. Conclusions : These results show that RD treatment can recover abnormal calcium metabolic process by sex hormone inequality, promoting bone formation and inhibiting bone absorption.

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In Vitro Evaluation of Three Positional Isomers of mono-PEGylated Salmon Calcitonin

  • Jung, J.Y.;Youn, Y.S.;Oh, S.H.;Hong, S.T.;Lee, J.E.;Lee, S.O.;Lee, K.C.
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.300.1-300.1
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    • 2003
  • Salmon calcitonin (sCT) is a therapeutic polypeptide hormone consisting of 32 amino acids (3432 Da). As with other bioactive peptide therapeutics, however, therapeutic use of sCT has been limited due to the problems of short circulating half-life and rapid proteolytic degradation. To get over this problem, the three positional isomers of mono-PEGylated sCT were prepared and among these, the best drug candiate for nasal application was chosen. (omitted)

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