• 한국작물학회지
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• 제64권3호
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• pp.246-268
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• 2019

Soybean [Glycine max (L.) Merr.] is a species of legume native to East Asia. The interactions between climatic conditions and genetic characteristics are known to affect the agricultural performance of soybean. Therefore, the present investigation was conducted to identify the main elements affecting the agricultural performances of 11 soybean varieties/lines from China [Harbin ($45^{\circ}12^{\prime}N$), Yanji ($42^{\circ}53^{\prime}N$), Dalian ($39^{\circ}30^{\prime}N$), Qingdao ($36^{\circ}26^{\prime}N$)] and the Republic of Korea [Suwon ($37^{\circ}16^{\prime}N$), and Jeonju ($35^{\circ}49^{\prime}N$)]. The days to flowering (DTF) of soybeans with the e1-nf and e1-as alleles and the E1e2e3e4 genotype, except in 'Keumgangkong', 'Tawonkong', and 'Duyoukong', were relatively short compared to those of soybeans with other alleles. Although DTF of the soybeans was highly correlated with all climatic conditions [negative: precipitation, average temperature (AVT), accumulated temperature; positive: day-length (DL)], days to maturity and 100-seed weight of the soybeans showed no significant correlation with any climatic conditions. The soybeans with a dominant Dt1 allele, except 'Tawonkong', had the longest stem length (STL). Moreover, STL of the soybeans grown in the test fields showed a positive correlation with only DL; however, the results of our chamber test that was conducted to complement the field tests showed that STL of soybean was positively affected by AVT and DL. Although soybean yield (YLD) showed positive correlations with latitude and DL (except L62-667, OT89-5, and OT89-6), the response of YLD to the climatic conditions was cultivar-specific. Our results show that DTF and STL of soybeans grown in six different latitudes are highly affected by DL, and AVT and genetic characteristic also affect DTF and STL.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 2002년도 학술대회지
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• pp.96-112
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• 2002

In the present study, we have investigated the effects of centrally administered ginsenoside Rc or Rgl on the modulation of NMDA receptor and $GABA_A$ receptor binding in rat brain. The NMDA receptor binding was analyzed by quantitative autoradiography using $[^3H]MK-801$ binding, and $GABA_A$ receptor bindings were analyzed by using $[^3H]muscimol\;and\;[^3H]flunitrazepam$ in rat brain slices. Rats were infused with ginsenoside Rc or Rg1 ($10\;{\mu}g/10{\mu}l/hr$, i.c.v.) for 7 days, through pre-implanted cannula by osmotic minipumps (Alzet, model 2ML), The levels of $[^3H]MK-801$ binding were highly decreased in part of cortex and cingulated by ginsenoside Rc and Rgl. The levels of $[^3H]muscimol$ binding were strongly elevated in almost all regions of frontal cortex by the treatment of ginseoside Rc but decreased by ginsenoside Rg 1. However, the $[^3H]flunitrazepam$ binding was not modulated by ginsenoside Rc or ginsenoside Rgl infusion. These results suggest that prolonged infusion of ginsenoside could differentially modulate $[^3H]MK-801\;and\;[^3H]muscimol$ binding in a region-specific manner. Also, we investigated the influence of centrally administered ginsenoside on the regulation of mRNA levels of the family of NMDA receptor subtypes (NR1, NR2A, NR2B, NR2C) by in situ hybridization histochemistry in the rat brain. The level of NR1 mRNA is significantly increased in temporal cortex, caudate putamen, hippocampus, and granule layer of cerebellum in Rgl-infused rats as compared to control group. The level of NR2A mRNA is elevated in the frontal cortex. In contrast, it was decreased in CAI area of hippocampus in Rgl-infused rats. However, there was no significant change of NR1 and NR2A mRNA levels in Rc-infused rats. The level of NR2B mRNA is elevated in cortex, caudate putamen, and thalamus in both Rc- and Rg-infused rats. In contrast, NR2B level is decreased in CA3 in Rgl-infused rats. The level of NR2C mRNA is increased in the granule layer of cerebellum in only Rg1 but not Rc infused rats. These results show that structure difference of ginsenoside may diversely affect the modulation of expression of NMDA receptor subunit mRNA after infusion into cerebroventricle in rats.

• 암석학회지
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• 제23권3호
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• pp.273-277
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• 2014

이 논문은 지구의 나이를 알기 위한 초기의 다양한 시도를 간략히 소개하고, 운석의 납동위원소 연대측정으로 처음으로 지구의 나이가 $4,550{\pm}70Ma$임을 밝힌 Patterson (1956)의 연구를 재조명한다. 태양계 초기의 진화과정은 성운가스가 식으면서 응축되어 만들어지는 고체입자 -> 이들 입자들이 서로 들러붙어 커지는 첨합과정을 통하여 행성 크기의 물체가 생성되는 것으로 생각되고 있다. 또한 이때 생긴 원시지구가 화성 크기의 물체와 충돌하면서 그 잔해가 달을 만들었다고 생각되고 있다. 이 일련의 과정에서 지구가 생성된 시기를 꼭 집어 말하기 힘들긴 하나, 현재 지구는 이 충돌의 직접적인 결과로 생각할 수 있기 때문에 이 충돌시기를 지구의 나이로 정할 것을 제안한다. 기존 연구를 고려하면 충돌시기는 태양계에서 가장 먼저 만들어진 운석물질의 나이(즉, 태양계의 나이) $4567.30{\pm}0.16Ma$와 지구와 달 암석에서 가장 오래된 나이 $4,456{\pm}40Ma$ 사이로 개략적으로 제한될 수 있다. 이 충돌시기는 태양계 초기 행성 크기의 물체가 만들어지는 시간간격을 밝히고, 충돌 이후 생성된 지구와 달의 마그마 바다의 열 역사를 규명하는데 매우 중요하기 때문에, 앞으로 보다 정확한 충돌시기를 측정하려는 노력이 요구된다.

• Nuclear Engineering and Technology
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• 제53권10호
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• pp.3217-3228
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• 2021

Flow blockage of the fuel assembly in the lead-based fast reactor (LFR) may produce critical local spots, which will result in cladding failure and threaten reactor safety. In this study, the flow blockage characteristics were analyzed with the sub-channel analysis method, and the circumferentially-varied method was employed for considering the non-uniform distribution of circumferential temperature. The developed sub-channel analysis code SACOS-PB was validated by a heat transfer experiment in a blocked 19-rod bundle cooled by lead-bismuth eutectic. The deviations between the predicted coolant temperature and experimental values are within ±5%, including small and large flow blockage scenarios. And the temperature distributions of the fuel rod could be better simulated by the circumferentially-varied method for the small blockage scenario. Based on the validated code, the analysis of blockage characteristics was conducted. It could be seen from the temperature and flow distributions that a large blockage accident is more destructive compared with a small one. The sensitivity analysis shows that the closer the blockage location is to the exit, the more dangerous the accident is. Similarly, a larger blockage length will lead to a more serious case. And a higher exit temperature will be generated resulting from a higher peak coolant temperature of the blocked region. This work could provide a reference for the future design and development of the LFR.

• 한국산학기술학회논문지
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• 제22권4호
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• pp.554-562
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• 2021

본 연구는 김치로부터 β-glucosidase 생산하는 Lactobacillus plantarum SM4를 분리하여 흰 민들레 발효를 수행하고 미백과 주름개선 효과를 가지는 생리활성 평가를 위해 총 폴리페놀 함량, 플라보노이드 함량, 항산화 활성, 타이로시네이즈 활성저해, 콜라게네이즈 활성저해, 자외선 차단율을 분석하였다. 흰 민들레 발효물의 총 폴리페놀 함량이 41.8±0.26 mg GAE/g DW로 열수추출물 21.4±0.67 mg GAE/g DW에 비해 2배 가량 증가하였으며 항산화 활성은 65.6±4.7%로 4배 증가하였다. 미백과 주름개선의 지표인 타이로시나아제 및 콜라게네이즈 활성저해에 있어 흰 민들레 발효물이 87.9±4.73%와 66.7±3.48%로 열수추출물에 비해 각각 2.4배와 1.5배 증가한 결과를 보였다. 흰 민들레 발효물과 열수추출물의 자외선 차단 효과를 비교하였을 때, UVA(320 nm) 차단율이 각각 64.7%와 15.2%로 측정되어 흰 민들레 발효물이 열수추출물에 비해 더 높은 효과를 나타내어 김치로부터 분리한 L. plantarum SM4가 생산하는 β-glucosidase를 발효를 통해 피부 미백, 주름 방지, 자외선 차단 효과가 증진됨을 확인하였다.

• Journal of Breast Cancer
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• 제21권4호
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• pp.371-381
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• 2018

Purpose: Immune suppression is common in patients with advanced breast cancer but the mechanisms underlying this phenomenon have not been sufficiently studied. In this study, we aimed to identify B7 family members that were able to predict the immune status of patients, and which may serve as potential targets for the treatment of breast cancer. We also aimed to identify microRNAs that may regulate the expression of B7 family members. Methods: The Cancer Genome Atlas data from 1,092 patients with breast cancer, including gene expression, microRNA expression and survival data, were used for statistical and survival analyses. Polymerase chain reaction and Western blot were used to measure messenger RNA and protein expression, respectively. Luciferase assay was used to investigate direct microRNA target. Results: Bioinformatic analysis predicted that microRNA (miR)-93, miR-195, miR-497, and miR-340 are potential regulators of the immune evasion of breast cancer cells, and that they exert this function by targeting CD274, PDCD1LG2, and NCR3LG1. We chose CD274 for further investigations. We found that miR-195, miR-497, and CD274 expression levels were inversely correlated in MDA-MB-231 cells, and miR-195 and miR-497 expressions mimic inhibited CD274 expression in vitro. Mechanistic investigations demonstrated that miR-195 and miR-497 directly target CD274 3' untranslated region. Conclusion: Our data indicated that the level of B7 family members can predict the prognosis of breast cancer patients, and miR-195/miR-497 regulate CD274 expression in triple negative breast cancer. This regulation may further influence tumor progression and the immune tolerance mechanism in breast cancer and may be able to predict the effect of immunotherapy on patients.

• Biomolecules & Therapeutics
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• 제27권3호
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• pp.327-335
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• 2019

As the elderly population is increasing, Alzheimer's disease (AD) has become a global issue and many clinical trials have been conducted to evaluate treatments for AD. As these clinical trials have been conducted and have failed, the development of new theraphies for AD with fewer adverse effects remains a challenge. In this study, we examined the effects of Theracurmin on cognitive decline using 5XFAD mice, an AD mouse model. Theracurmin is more bioavailable form of curcumin, generated with submicron colloidal dispersion. Mice were treated with Theracurmin (100, 300 and 1,000 mg/kg) for 12 weeks and were subjected to the novel object recognition test and the Barnes maze test. Theracurmin-treated mice showed significant amelioration in recognition and spatial memories compared those of the vehicle-treated controls. In addition, the antioxidant activities of Theracurmin were investigated by measuring the superoxide dismutase (SOD) activity, malondialdehyde (MDA) and glutathione (GSH) levels. The increased MDA level and decreased SOD and GSH levels in the vehicle-treated 5XFAD mice were significantly reversed by the administration of Theracurmin. Moreover, we observed that Theracurmin administration elevated the expression levels of synaptic components, including synaptophysin and post synaptic density protein 95, and decreased the expression levels of ionized calcium-binding adapter molecule 1 (Iba-1), a marker of activated microglia. These results suggest that Theracurmin ameliorates cognitive function by increasing the expression of synaptic components and by preventing neuronal cell damage from oxidative stress or from the activation of microglia. Thus, Theracurmin would be useful for treating the cognitive dysfunctions observed in AD.

• Steel and Composite Structures
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• 제30권6호
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• pp.551-565
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• 2019

A total of 36 carbon steel and stainless steel bolted connections subjected to shear loading at different strain rates was experimentally investigated. The connection specimens were fabricated from carbon steel grades 1.20 mm G500 and 1.90 mm G450, as well as cold-formed stainless steel types EN 1.4301 and EN 1.4162 with nominal thickness 1.50 mm. The connection tests were conducted by displacement control test method. The strain rates of 10 mm/min and 20 mm/min were used. Structural behaviour of the connection specimens tested at different strain rates was investigated in terms of ultimate load, elongation corresponding to ultimate load and failure mode. Generally, it is shown that the higher strain rate on the bolted connection specimens, the higher ultimate load was obtained. The ultimate loads were averagely 2-6% higher, while the corresponding elongations were averagely 8-9% higher for the test results obtained from the strain rate of 20 mm/min compared with those obtained from the lower strain rates (1.0 mm/min for carbon steel and 1.5 mm/min for stainless steel). The connection specimens were generally failed in plate bearing of the carbon steel and stainless steel. It is shown that increasing the strain rate up to 20 mm/min generally has no effect on the bearing failure mode of the carbon steel and stainless steel bolted connections. The test strengths and failure modes were compared with the results predicted by the bolted connection design rules in international design specifications, including the Australian/New Zealand Standard (AS/NZS4600 2018), Eurocode 3 - Part 1.3 (EC3-1.3 2006) and North American Specification (AISI S100 2016) for cold-formed carbon steel structures as well as the American Specification (ASCE 2002), AS/NZS4673 (2001) and Eurocode 3 - Part 1.4 (EC3-1.4 2015) for stainless steel structures. It is shown that the AS/NZS4600 (2018), EC3-1.3 (2006) and AISI S100 (2016) generally provide conservative predictions for the carbon steel bolted connections. Both the ASCE (2002) and the EC3-1.4 (2015) provide conservative predictions for the stainless steel bolted connections. The EC3-1.3 (2006) generally provided more accurate predictions of failure mode for carbon steel bolted connections than the AS/NZS4600 (2018) and the AISI S100 (2016). The failure modes of stainless steel bolted connections predicted by the EC3-1.4 (2015) are more consistent with the test results compared with those predicted by the ASCE (2002).

• 한국초등수학교육학회지
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• 제22권3호
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• pp.309-330
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• 2018

본 연구의 목적은 구조화 정도가 서로 다른 문제제기 과제를 제시한 후 학생들의 수학 문제제기를 집단별로 분석하여 문제제기 능력이 영재를 판별하는 데 유효한 변인이 될 수 있는지 그 가능성을 확인하는 것이다. 그리고 이를 바탕으로 수학적 창의성을 신장시키기 위한 초등수학영재교육의 방향을 제시하고자 한다. 본 연구에는 영재학생 47명과 일반학생 47명이 참여하여 Stoyanova와 Ellerton(1996)의 구분에 따른 비구조화 및 구조화 문제제기 과제를 수행하였으며, 그 결과를 분석기준에 따라 분석하였다. 수학 문제제기 능력을 측정하기 위한 분석기준으로 Silver와 Cai(2005)가 제안한 유창성, 독창성, 언어적 복잡성, 수학적 복잡성에 Yuan과 Sriraman(2010)의 융통성을 추가하여 기본 분석틀로 구성하였다. 그리고 여기에 수학적 복잡성을 보완하기 위한 기준으로 풀이의 단계적 깊이를 추가하였다. 연구 결과, 과제의 구조화 정도에 상관없이 영재학생은 일반학생에 비하여 수와 연산 영역의 문제를 적게, 도형 영역의 문제는 더 많이 제기하였다. 구조화 정도가 서로 다른 과제의 문제제기에서 영재학생과 일반학생을 판별할 수 있는 공통된 지표는 독창성과 풀이의 단계적 깊이의 두 가지로 나타났다. 한편, 풀이의 단계적 깊이가 3이상인 문제는 독창적인 문제일 가능성이 높은 것으로 나타나, 학생들의 창의적 문제제기 활동을 지도할 때에는 단순히 연산이 많은 문제가 아닌, 다중단계의 문제를 만들도록 격려해야 필요가 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1145-1152
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• 2019

Objective: Mulberry (Morus alba L.) is a cultivated shrub grown widely in the sub-tropical and tropical areas. It has been shown that mulberry leaf contains high levels of protein while having polyphenols as phytonutrients. Therefore, it is important to conduct an experiment to assess potential toxic level from mulberry on behavior, blood hematological and coagulation parameter using Sprague-Dawley (SD) rats. Methods: Both male and female SD rats were given an intragastric administration of respective treatments of mulberry leaf intakes (control, low and high levels). Parameters of feed intake, hematological and coagulation of blood parameters, as well as liveweight changes were taken during the 7 d of adaptation, 28 d of treatment exposure, and 14 d of recovery periods, respectively. All treatment data were statistically analyzed using analysis of variance of SPSS17.0 for Windows Statistical Software following the Randomized complete block design with sex as a block. Results: Most of the parameters of the physical symptoms of the SD rats, were not significantly different (p>0.05) when compared with that of the control group. Those which remain unchanged in each dose group were, body weight (BW) gain, feed intake, the hematology and coagulation indexes. Although, there were a few individual indicators that were abnormal, but the overall physiological appearance of the rats were normal. Conclusion: Results under this experiment revealed that most hematological and coagulation parameters of the SD rats in both male and female were normal, although the weight gain of female rats in high-dose group was significantly reduced than those of the male rats. Under this study, the use of mulberry leaf up to 2 g/kg BW did not result in abnormal phenomenon in the SD rats. These findings would offer useful information for further in vivo feeding trials in animals to extensively use of mulberry leaf to improve animal production, particularly in P.R. China.