• Title/Summary/Keyword: CSL

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Fed-Batch Fermentation of High-Content RNA Yeast by Using Molasses Medium. (당밀 배지를 이용한 고함량 RNA효모의 유가배양)

  • 김재범;권미정;남희섭;김재훈;남수완
    • Microbiology and Biotechnology Letters
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    • v.29 no.4
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    • pp.234-239
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    • 2001
  • In order to maximize the RNA accumulation and biomass production is Saccharomyces cerevisiae MTY62, a high-content RNA yeast strain, batch and fed-batch cultures were performed. Among the feeding modes of fed-batch cultures examined, the intermittent feeding mode R\`(IFB-lV), in which 50 ml of 40% molasses and 20% com steep liquor (CSL) solution was intermittently fed for 5 times, resulted in the cell concentration of 33.8 g- dry cell weight/1 and the RNA concentration of 5221 mg-/l, and RNA content of 153 mg-RNA/g-dry cell weight. The constant fed-batch with feeding mode III (CFB-III), in which the feeding rate of 40% molasses and 20% CSL solution was stepwisely decreased from 48 mph (9-13 h), to 24 mph (13-21 h), and to 18 ml/h (21∼ 48 h), gave the highest cell concentration of 42.7 g-dry ceil weigh71 and R7IA concentration of 5536 mg-RNA/1, which were about 2.4-fold and 1.9-fold increased levels, respectively, compared to the results of batch culture. However, the RNA con- tent of 130 mg-RNA/g-dry cell weight of the fed-batch was lower than that of the batch culture (171 mg-RNA/g-dry cell weight) and other fed-batch cultures. When the specific growth rates in the fed-batch cultures were increased, the RNA contents increased. This result indicates that the RNA content is adversely proportional to the cell concen- tration. However, at the same specific growth rate, the RNA content was maintained at higher level in the intermit- tent fed-batch than in the constant fed-batch culture.

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A Study on the Correlation Between Sasang Constitution and Sound Characteristics Used Harmonics and Formant Bandwidth (Harmonics(배음)와 Formant Bandwidth(포먼트 폭)를 이용한 음성특성(音聲特性)과 사상체질간(四象體質間)의 상관성(相關性) 연구(硏究))

  • Park, Sung-Jin;Kim, Dal-Rae
    • Journal of Sasang Constitutional Medicine
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    • v.16 no.1
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    • pp.61-73
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    • 2004
  • This study was prepared to investigate the correlation between Sasang constitutional groups and voice characteristics using voice analysis system(in this study, CSL). I focused on the voice characteristics in terms of harmonics, Formant frequency and Formant Bandwidth. The subjects were 71 males. I classified them into three groups, that is Soeumin group, Soyangin group and Taeumin group. The classification method of Constitution used two ways, QSCCII(Questionnarie for the Sasang Constitution Classification II) and Interview with a specialist in Sasang Constitution. So 71 people were categorized into 31 Soeumin(people), 18 Soyangin(people) and 22 Taeumin(people). Pitch is approximately similar to the fundamental frequency(F0) in voices. Shimmer in dB gives an evaluation of the period-to-period variability of the peak-to-peak amplitude within the analyzed voice sample. FFT(Fast Fourier Transform) method in CSL can display sampled voices into harmonics. H1 is the first peak and h2 is the second peak in the harmonics. The amplitude difference of h1 and h2(h1-h2) can be explained as the speaker's phonation type, And Formant frequency and bandwidth can be explained as the speaker's vocal tract. So I checked the harmonics and Formant frequency and Bandwidth as the voice parameters. First I have captured /e/ voices from all subjects using microphone. And then I analyzed /e/ voices with CSL. Power Spectrum and Formant History is the menu in the CSL which can display harmonics and Formant frequency and bandwidth. The results about the correlation between Sasang Constitutional Groups and voice parameters are as follows; 1. There is no significant amplitude difference of harmonics(h1-h2) among three groups. 2. There is the significant difference between Soeumin Group and Soyangin Group in Formant Frequency 1 and Formant Bandwidth 1(p<0.05). Any other parameters have no significance. I assume that Soyangin Group has clearer and brighter voice than Soeumin Group according to the Formant Bandwidth difference. And I think its result has coincidence with the context of "Dongyi Suse Bowon" and "Sasangimhejinam".

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Characteristics of Grain Orientation and Grain Boundaries of the $ZrB_2$-ZrC Composites Densified by Spark Plasma Sintering (방전플라즈마소결법으로 제조된 $ZrB_2$-ZrC 복합체의 결정립 방위분포 및 결정입계의 특성)

  • Shim, Seung-Hwan;Kim, Kyoung-Hun;Shim, Kwang-Bo
    • Journal of the Korean Ceramic Society
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    • v.38 no.10
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    • pp.914-920
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    • 2001
  • The grain orientation distribution and grain boundary characterization of $ZrB_2$-ZrC composites sintered by a SPS(Spark Plasma Sintering) method, a new sintering technique were analyzed by the EBSP technique and then their crystallographic results have been compared with those of a sintered specimen using a PLS(Pressureless Sintering) method. In the $ZrB_2$-ZrC composite manufactured by SPS, (0001) planes of $ZrB_2$ were oriented in the direction normal to the specimen surface. In the case of PLS, those of $ZrB_2$ were oriented normal to the electron beam. In both cases of PLS and SPS, ZrC grains had the randomly oriented grain structure. The grain boundary characterization showed that low angle grain boundaries in the PLS and SPS processed materials constituted about 10% and 8% of the total number of boundaries, respectively, represented the only slight difference between the proportion of low angle grain boundary. However, in the distribution of CSL(Coincident Site Lattice) boundaries, it was shown the higher proportion of CSL boundaries with $\Sigma$ 3,5,7,9, 11 in the SPS processed material.

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Production of Organic Acids from Food By-Products - Mass Production of Organic Acids by Continuous Flow Ceil Recycling Fermentation - (식품부산물로부터 유기산의 대량생산공정에 관한 연구 - 세포재순환식 연속발효를 이용한 유기산의 대량 생산 -)

  • Ju Yun-Sang;Jin Sun-Ja;Hwang Pil-Gi;Choi Chul-Ho;Lee Eui-Sang
    • KSBB Journal
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    • v.19 no.6 s.89
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    • pp.484-488
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    • 2004
  • Fermentation studies were conducted in batch and continuous flow cell-recycle reactors with food by-products as substrates. The genus Propionibacterium acidipropionici ATCC 4965 was utilized in the production of organic acids. Good performance was achieved in the batch fermentation using hydrol as a carbon source and corn steep liquor (CSL) as nitrogen and vitamin sources. Product yields and productivity based on maximum values were 0.80 g total acids/g glucose and 0.26 g total acids/L/h, respectively, when $3\%$, (w/v) of hydrol and $2.5\%$, (w/v) of CSL were utilized. Continuous fermentation with cell-recycling system using the optimum amounts of substrates resulted in dramatic increase in cell concentration (X) and maximum productivity (P). Compared to the batch fermentation, X and P were increased by as much as 21 and 13 times, respectively, at the dilution ratio of $0.2\;hr^{-1}$, indicating that cell recycling fermentation of food by-products provides valuable means for the mass production of organic acids as well as utilizing cell mass as good nutrient resources.

The Effect of Inter-word Space on Chinese reading: An Eye Movement Study (단어 간 공백이 중국어 글 읽기에 미치는 영향: 안구운동 추적 연구)

  • Han, Mi-ae;Jiang, Xin;Zhao, Weiqi
    • Korean Journal of Cognitive Science
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    • v.29 no.4
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    • pp.243-263
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    • 2018
  • This research investigated whether inter-word spaces, the spaces between words, can affect the efficiency of Korean-speaking CSL(Chinese as a second language) learners in Chinese reading of Korean-speaking's ability to read Chinese. Through eye movement tracking experiments, CSL learners of different proficiency levels(beginning, intermediate, and advanced) and native Chinese readers were asked to read Chinese sentences with and without inter-word spaces. The tests analysed the participants' fixation counts and the time spent in reading each sentences and also between each words. In terms of the fixation counts and time spent between sentences, the results show that there were no significant difference in participants' fixation counts from reading sentences with and without inter-word spaces. The results also prove that reading sentences with inter-word spaces significantly shortened the reading time for both CSL learners and native Chinese readers. Even for the participants' fixation counts and time duration between each words, participants spent significantly less fixation counts and reading time while reading words with inter-word spaces. The results were more prominent and positive in tests conducted with CSL learners of lower proficiency. This research shows that inter-word spaces in Chinese texts can enhance the efficiency of chinese learners' reading ability.

Isometries of $B_{2n - (T_0)}

  • Park, Taeg-Young
    • Journal of the Korean Mathematical Society
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    • v.32 no.3
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    • pp.593-608
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    • 1995
  • The study of self-adjoint operator algebras on Hilbert space is well established, with a long history including some of the strongest mathematicians of the twentieth century. By contrast, non-self-adjoint CSL-algebras, particularly reflexive algebras, are only begins to be studied by W. B. Wrveson [1] 1974.

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The Improvement of Cephalosporin C Production by Fed-batch Culture of Cephalosporium acremonium M25 Using Rice Oil

  • Kim Jin Hee;Lim Jung Soo;Kim Seung Wook
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.459-464
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    • 2004
  • The objective of this study is to improve cephalosporin C (CPC) production byoptimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and $6.68\%$, respectively. CPC produc­tion was improved $50\%$ by feeding of $5\%$ rice oil at day 3rd and 5th day during the shake flask culture of C acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bio­reactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about $132\%$ compared to the re­sult with batch flask culture.

Normal Interpolation on AX = Y in CSL-algebra AlgL

  • Jo, Young Soo;Kang, Joo Ho
    • Kyungpook Mathematical Journal
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    • v.45 no.2
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    • pp.293-299
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    • 2005
  • Let ${\cal{L}}$ be a commutative subspace lattice on a Hilbert space ${\cal{H}}$ and X and Y be operators on ${\cal{H}}$. Let $${\cal{M}}_X=\{{\sum}{\limits_{i=1}^n}E_{i}Xf_{i}:n{\in}{\mathbb{N}},f_{i}{\in}{\cal{H}}\;and\;E_{i}{\in}{\cal{L}}\}$$ and $${\cal{M}}_Y=\{{\sum}{\limits_{i=1}^n}E_{i}Yf_{i}:n{\in}{\mathbb{N}},f_{i}{\in}{\cal{H}}\;and\;E_{i}{\in}{\cal{L}}\}.$$ Then the following are equivalent. (i) There is an operator A in $Alg{\cal{L}}$ such that AX = Y, Ag = 0 for all g in ${\overline{{\cal{M}}_X}}^{\bot},A^*A=AA^*$ and every E in ${\cal{L}}$ reduces A. (ii) ${\sup}\;\{K(E, f)\;:\;n\;{\in}\;{\mathbb{N}},f_i\;{\in}\;{\cal{H}}\;and\;E_i\;{\in}\;{\cal{L}}\}\;<\;\infty,\;{\overline{{\cal{M}}_Y}}\;{\subset}\;{\overline{{\cal{M}}_X}}$and there is an operator T acting on ${\cal{H}}$ such that ${\langle}EX\;f,Tg{\rangle}={\langle}EY\;f,Xg{\rangle}$ and ${\langle}ET\;f,Tg{\rangle}={\langle}EY\;f,Yg{\rangle}$ for all f, g in ${\cal{H}}$ and E in ${\cal{L}}$, where $K(E,\;f)\;=\;{\parallel}{\sum{\array}{n\\i=1}}\;E_{i}Y\;f_{i}{\parallel}/{\parallel}{\sum{\array}{n\\i=1}}\;E_{i}Xf_{i}{\parallel}$.

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Speech Rates of Male Esophageal Speech (식도발성 남성 발화의 말 속도)

  • Park, Won-Kyoung;Shim, Hee-Jeong;Ko, Do-Heung
    • Phonetics and Speech Sciences
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    • v.4 no.3
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    • pp.143-149
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    • 2012
  • The purpose of this study is to investigate the speech rate of an esophageal speech group that is capable of vocalization after surgery. The subjects in this experiment were 10 male esophageal speakers and 10 male laryngeal speakers. Each group read a reading passage that was recorded by a DAT recorder (Rolando, EDIROL R-09). These records were analyzed by using CSL (Computerized Speech Lab, model 4150). The results were as follows: (1) the overall speech rate of esophageal speech was 2.50 SPS (syllable per second) while the overall speech rate of laryngeal speech was 4.23 SPS. (2) The articulatory rate of esophageal speech was 3.14 SPS (syllable per second) while the articulatory rate of laryngeal speech was 4.75 SPS. Speech rates as well as articulatory rates of esophageal speech were significantly lower than laryngeal speech. These differences between the two groups may be due to reduced efficiency of airflows across the pharyngeal-esophageal segment for esophageal speakers when compared to airflow through the glottis for laryngeal speakers. These results would provide a guideline in speech rates for esophageal speakers in clinical settings.