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The Improvement of Cephalosporin C Production by Fed-batch Culture of Cephalosporium acremonium M25 Using Rice Oil  

Kim Jin Hee (Department of Chemical and Biological Engineering, Korea University)
Lim Jung Soo (Department of Chemical and Biological Engineering, Korea University)
Kim Seung Wook (Department of Chemical and Biological Engineering, Korea University)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.9, no.6, 2004 , pp. 459-464 More about this Journal
Abstract
The objective of this study is to improve cephalosporin C (CPC) production byoptimization of medium and culture conditions. A statistical method was introduced to optimize the main culture medium. The main medium for CPC production was optimized using a statistical method. Glucose and corn steep liquor (CSL) were found to be the most effective factors for CPC production. Glucose and CSL were optimized to 2.84 and $6.68\%$, respectively. CPC produc­tion was improved $50\%$ by feeding of $5\%$ rice oil at day 3rd and 5th day during the shake flask culture of C acremonium M25. The effect of agitation speeds on CPC production in a 2.5-L bio­reactor was also investigated with fed-batch mode. The maximum cell mass (54.5 g/L) was obtained at 600 rpm. However, the maximum CPC production (0.98 g/L) was obtained at 500 rpm. At this condition, the maximum CPC production was improved about $132\%$ compared to the re­sult with batch flask culture.
Keywords
cephalosporin C; Cephalosporium acremonium; optimization; statistical method; rice oil;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 11  (Related Records In Web of Science)
Times Cited By SCOPUS : 10
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